TY - GEN A1 - Alipoursarbani, Mozhdeh A1 - Abendroth, Christian ED - Bockreis, Anke ED - Faulstich, Martin ED - Flamme, Sabine ED - Kranert, Martin ED - Mocker, Mario ED - Nelles, Michael ED - Quicker, Peter ED - Rettenberger, Gerhard ED - Rotter, Vera Susanne T1 - Aerobic Digestate Reactivation of Anaerobically Digested Sewage Sludge – A Micro Review T2 - Kreislauf und Ressourcenwirtschaft : 13.Wissenschaftskongress : am 15. und 16. Februar 2024 an der Technischen Universität Wien N2 - The authors of the present work summarize their recently published article “Microbiome Characterization after Aerobic Digestate Reactivation of Anaerobically Digested Sewage Sludge” from Otto et al. (2023). This article presents the outcomes of a municipal-scale demonstrator plant implementing a recently patented process for improved sludge degradation within a municipal sewage treatment system. The study focused on a 1500 m³ sewage sludge digester, where an intermediate aerobic sewage sludge reactivation stage was introduced. This reactivation resulted in a 55% increase in biogas production and a 25% reduction in residual solids. For the residual NH4 in the liquid phase of the sewage sludge, the process achieved NH4-N removal rates exceeding 90%. To gain insights into the microbial community dynamics, 16S rRNA gene amplicon high-throughput sequencing was employed on the reactivated digestate. The analysis revealed a reduced population of methane-forming archaea compared to the primary digester, along with the detection of multiple ammonium-oxidizing bacteria. Notably, genera within the Chitinophagaceae family, comprising up to 18.8% of DNA sequences, were prevalent, alongside a small presence of Candidatus nitrosoglobus (<0.3%). The study highlights an economically viable approach for efficient nitrogen removal from sewage sludge while simultaneously increasing biogas yields and reducing potential pathogens in the residual material. KW - Biogas KW - Mikrobiome KW - Sewage Sludge Y1 - 2024 SN - 978-3-99106-120-5 U6 - https://doi.org/10.15203/99106-120-5 SP - 177 EP - 180 PB - Innsbruck university press CY - Innsbruck ER - TY - GEN A1 - Otto, Pascal A1 - Alipoursarbani, Mozhdeh A1 - Torrent, Daniel A1 - Latorre-Pérez, Adriel A1 - Paust, Thomas A1 - Albert, Alfred A1 - Abendroth, Christian T1 - Microbiome Characterization after Aerobic Digestate Reactivation of Anaerobically Digested Sewage Sludge T2 - Fermentation N2 - A demonstrator plant of a recently patented process for improved sludge degradation has been implemented on a municipal scale. In a 1500 m3 sewage sludge digester, an intermediary stage with aerobic sewage sludge reactivation was implemented. This oxic activation increased the biogas yield by up to 55% with a 25% reduction of the remaining fermentation residue volume. Furthermore, this process allowed an NH4-N removal of over 90%. Additionally, 16S rRNA gene amplicon high-throughput sequencing of the reactivated digestate showed a reduced number of methane-forming archaea compared to the main digester. Multiple ammonium-oxidizing bacteria were detected. This includes multiple genera belonging to the family Chitinophagaceae (the highest values reached 18.8% of the DNA sequences) as well as a small amount of the genus Candidatus nitrosoglobus (<0.3%). In summary, the process described here provides an economically viable method to eliminate nitrogen from sewage sludge while achieving higher biogas yields and fewer potential pathogens in the residuals. KW - anaerobic digestion KW - anaerobic microbiomes KW - aerobic sludge activation KW - 16S rRNA sequencing KW - water treatment Y1 - 2023 U6 - https://doi.org/10.3390/fermentation9050471 SN - 2311-5637 VL - 9 IS - 5 ER -