TY - GEN A1 - Olowe, Olugbenga Adekunle A1 - Choudhary, S. A1 - Schierack, Peter A1 - Wieler, Lothar H. A1 - Makanjuola, O. B. A1 - Olayemi, A. B. A1 - Anjum, M. T1 - Pathotyping bla CTX-M Escherichia coli from Nigeria T2 - European Journal of Microbiology and Immunology N2 - Background: Escherichia coli have become the enterobacteriaceae species most affected by extended-spectrum β-lactamases (ESBLs) in view of the emergence of CTX-M-type ESBLs. These CTX-M-positive E. coli have been reported in numerous regions worldwide. Virulence determinants of already reported CTX-M-positive E. coli were investigated. Methodology: To gain insights into the mechanism underlying this phenomenon, we assessed serogroup, susceptibility pattern and diversity of virulence profiles within a collection of nine blaCTX-M-positive E. coli strains and their virulent determinant using miniaturized DNA microarray techniques. The nine ESBL-positive E. coli isolates were from eight male and one female patient(s) selected for study based on previous work. Virulence potential was inferred by detection of 63 virulence factor (VF) genes. Results: Four (44.4%) of the 9 E. coli isolates exhibited the same set of core characteristics: serotype O8:Hnt, while all were positive for OXA-1, ciprofloxacin resistance. Five of the isolates exhibited highly similar (91% to 100%) VF profiles. Conclusion: The findings describe a broadly disseminated, blaCTX-M-positive and virulent E. coli serogroup with highly homogeneous virulence genotypes, suggesting recent emergence in this zone. Understanding how this clone has emerged and successfully disseminated within the hospital and community, including across national boundaries, should be a public health priority. Y1 - 2013 U6 - https://doi.org/10.1556/EuJMI.3.2013.2.5 SN - 2062-8633 VL - 3 IS - 2 SP - 120 EP - 125 ER -