TY - GEN A1 - Scherbahn, Vitali A1 - Nizamov, Shavkat A1 - Mirsky, Vladimir M. T1 - Plasmonic detection and visualization of directed adsorption of charged single nanoparticles to patterned surfaces T2 - Microchimica Acta N2 - It has recently been shown that surface plasmon microscopy (SPM) allows single nanoparticles (NPs) on sensor surfaces to be detected and analyzed. The authors have applied this technique to study the adsorption of single metallic and plastic NPs. Binding of gold NPs (40, 60 and 100 nm in size) and of 100 nm polystyrene NPs to gold surfaces modified by differently ω-functionalized alkyl thiols was studied first. Self-assembled monolayers (SAM) with varying terminal functions including amino, carboxy, oligo(ethylene glycol), methyl, or trimethylammonium groups were deposited on gold films to form surfaces possessing different charge and hydrophobicity. The affinity of NPs to these surfaces depends strongly on the type of coating. SAMs terminated with trimethylammonium groups and carboxy group display highly different affinity and therefore were preferred when creating patterned charged surfaces. Citrate-stabilized gold NPs and sulfate-terminated polystyrene NPs were used as negatively charged NPs, while branched polyethylenimine-coated silver NPs were used as positively charged NPs. It is shown that the charged patterned areas on the gold films are capable of selectively adsorbing oppositely charged NPs that can be detected and analyzed with an ~1 ng⋅mL−1 detection limit. Y1 - 2016 U6 - https://doi.org/10.1007/s00604-016-1956-7 SN - 1436-5073 VL - 183 IS - 11 SP - 2837 EP - 2845 ER - TY - GEN A1 - Nizamov, Shavkat A1 - Kasian, Olga A1 - Mirsky, Vladimir M. T1 - Individual detection and electrochemically assisted identification of adsorbed nanoparticles using surface plasmon resonance microscopy T2 - Angewandte Chemie - International Edition N2 - The increasing production and application of nanoparticles necessitates a highly sensitive analytical method for the quantification and identification of these potentially hazardous materials. We describe here an application of surface plasmon microscopy for the individual detection of each adsorbed nanoparticle and for visualization of its electrochemical conversion. Whereas the adsorption rate characterizes the number concentration of nanoparticles, the potential at which the adsorbed nanoparticles disappear during an anodic potential sweep characterizes the type of material. All the adsorbed nanoparticles are subjected to the potential sweep simultaneously; nevertheless, each of the up to a million adsorbed nanoparticles is identified individually by its electrochemical dissolution potential. The technique has been tested with silver and copper nanoparticles, but can be extended to many other electrochemically active nanomaterials. Y1 - 2016 U6 - https://doi.org/10.1002/anie.201600853 SN - 1521-3773 VL - 55 IS - 25 SP - 7247 EP - 7251 ER - TY - GEN A1 - Sidorenko, Irina A1 - Nizamov, Shavkat A1 - Hergenröder, Roland A1 - Zybin, Alexander A1 - Kuzmichev, Alexei A1 - Kiwull, Bettina A1 - Niessner, Reinhard A1 - Mirsky, Vladimir M. T1 - Computer assisted detection and quantification of single adsorbing nanoparticles by differential surface plasmon microscopy T2 - Microchimica Acta N2 - Sensitive detection of engineered nanoparticles (NPs) in air and in liquid samples is an important task and still a major challenge in analytical chemistry. Recent work demonstrated that it can be performed using surface plasmon microscopy (SPM) where binding of single NPs to a surface leads to the formation of characteristic patterns in differential SPM images. However, these patterns have to be discriminated from a noisy background. Computer-assisted recognition of nanoparticles offers a solution but requires the development of respective tools for data analysis. Hereby a numerical method for automated detection and characterization of images of single adsorbing NPs in SPM image sequences is presented. The detection accuracy of the method was validated using computer generated images and manual counting. The method was applied for detecting and imaging of gold and silver NPs adsorbing from aqueous dispersions and for soot and NaCl NPs adsorbing from aerosols. The determined adsorption rate was in range 0.1–40 NPs per (s mm2) and linearly dependent on the concentration of nanoparticles. Depending on the type of NPs and signal to noise ratio, a probability of recognition of 90–95 % can be achieved. Y1 - 2016 U6 - https://doi.org/10.1007/s00604-015-1599-0 SN - 1436-5073 VL - 183 IS - 1 SP - 101 EP - 109 ER - TY - GEN A1 - Nizamov, Shavkat A1 - Scherbahn, Vitali A1 - Mirsky, Vladimir M. T1 - Self-referencing SPR-sensor based on integral measurements of light intensity reflected by arbitrarily distributed sensing and referencing spots T2 - Sensors and Actuators B: Chemical N2 - A new approach for self-referencing in SPR biosensors is reported. The method is technologically simple and applicable for a wide range of existing SPR instrumentation with Kretschmann configuration. It is based on the micropatterning of the sensor area with sensing and referencing areas whose shape and distribution can be chosen arbitrarily and their characteristic sizes are larger than the plasmons propagation length. Provided that roughly a half of the area is used as the sensing area while its optical thickness is different from that of the referencing area, an integral measurement of the intensity of the reflected light over such a patterned surface near the summary resonance conditions exhibits self-referencing properties. An over ten-fold suppression of the effect caused by the variation of the bulk refractive index was observed. Y1 - 2015 U6 - https://doi.org/10.1016/j.snb.2014.10.022 SN - 0925-4005 VL - 207 IS - A SP - 740 EP - 747 ER - TY - GEN A1 - Nizamov, Shavkat A1 - Mirsky, Vladimir M. T1 - Self-referencing SPR-biosensors based on penetration difference of evanescent waves T2 - Biosensors and Bioelectronics N2 - SPR based biosensors register binding of analytes to the surface with immobilized receptors by measuring changes of the refractive index near this surface. An important task in the improvement of this measurement technology is a separation of signals, corresponding to the changes in the chemosensitive layer, from undesired contributions of bulk phase, for example, due to fluctuations of temperature, concentrations of solutes, pressure. The wavelength of the incident light influences strongly the penetration depth of the corresponding evanescent wave. This dependence was exploited here for compensation of the contribution of the bulk refractive index. It was performed using differential SPR measurements at two wavelengths with differing penetration depths. Theoretical analysis and numerical optimization of the suggested approach, named a Penetration Difference Self-Referencing SPR (PDSR-SPR), were performed. Experimental test was performed using 658 and 980 nm laser diodes. Over 20 times suppression of variations of bulk refractive index with magnitude up to 1000 μRIU was observed. Finally, PDSR-SPR approach was applied for monitoring of antibodies binding to the immobilized antigens. KW - Surface plasmon resonance KW - SPR-biosenso KW - Evanescent wave KW - Affinity senso KW - Immunosensor Y1 - 2011 U6 - https://doi.org/10.1016/j.bios.2011.07.030 SN - 0956-5663 VL - 28 IS - 1 SP - 263 EP - 269 ER - TY - GEN A1 - Nizamov, Shavkat A1 - Scherbahn, Vitali A1 - Mirsky, Vladimir M. T1 - Detection and quantification of single engineered nanoparticles in complex samples using template matching in wide-field surface plasmon microscopy T2 - Analytical Chemistry N2 - An ultrasensitive analytical method for direct detection of single nanoparticles in complex environment is described. The method relies on the wide-field surface plasmon microscopy (SPM). The suppression of matrix effects is achieved by image analysis based on the template matching. First, characteristic SPM images of nanoparticles are collected in aqueous suspensions. Then the detection of nanoparticles in complex environment is performed using template matching. Quantification and characterization of nanoparticles size was demonstrated at subppb level (∼ 100 pg/mL) in such complex media as wines, fruit juices, or cosmetic formulation. Visualization of the nanoparticles is performed in real time. The method does not require any sample pretreatment. If the minimally acceptable adsorption rate is defined as one nanoparticle to the whole sensor surface per few seconds, the working range of the method is ∼ 10 6 to 10 10 nanoparticles per mL. Y1 - 2016 U6 - https://doi.org/10.1021/acs.analchem.6b02878 SN - 1520-6882 VL - 88 IS - 20 SP - 10206 EP - 10214 ER - TY - GEN A1 - Nizamov, Shavkat A1 - Scherbahn, Vitali A1 - Mirsky, Vladimir M. T1 - Ionic referencing in surface plasmon microscopy: visualization of the difference in surface properties of patterned monomolecular layers T2 - Analytical Chemistry N2 - An approach for visualization of patterned monomolecular layers in surface plasmon microscopy (SPM) is suggested. The development of hidden image in SPM is achieved by referencing of images obtained in the presence of electrolytes with a high molar refraction of either anions or cations. A formation of diffuse layer near the charged surface areas leads to the redistribution of ions. The ratio of SPM images allows one to visualize this redistribution and to distinguish surface areas with different properties. The approach is unobtrusive and robust; it can be used with most SPR imaging instruments. Y1 - 2017 U6 - https://doi.org/10.1021/acs.analchem.7b00251 SN - 0003-2700 SN - 1520-6882 VL - 89 IS - 7 SP - 3873 EP - 3878 ER - TY - GEN A1 - Laurinavichyute, Veronika K. A1 - Nizamov, Shavkat A1 - Mirsky, Vladimir M. T1 - The Role of Anion Adsorption in the Effect of Electrode Potential on Surface Plasmon Resonance Response T2 - ChemPhysChem : a European journal of physical chemistry and chemical physics N2 - Surface plasmon resonance, being widely used in bioanalytics and biotechnology, is influenced by the electrical potential of the resonant gold layer. To evaluate the mechanism of this effect, we have studied it in solutions of various inorganic electrolytes. The magnitude of the effect decreases according to the series: KBr>KCl>KF>NaClO4. The data were treated by using different models of the interface. A quantitative description was obtained for the model, which takes into account the local dielectric function of gold being affected by the free electron charge, diffuse ionic layer near the gold/water interface, and specific adsorption of halides to the gold surface with partial charge transfer. Taking into account that most biological experiments are performed in chloride-containing solutions, detailed analysis of the model at these conditions was performed. The results indicate that the chloride adsorption is the main mechanism for the influence of potential on the surface plasmon resonance. The dependencies of surface concentration and residual charge of chloride on the applied potential were determined. Y1 - 2017 U6 - https://doi.org/10.1002/cphc.201601288 SN - 1439-7641 SN - 1439-4235 VL - 18 IS - 12 SP - 1552 EP - 1560 ER - TY - CHAP A1 - Nizamov, Shavkat A1 - Mirsky, Vladimir M. ED - Kumar, Challa S. S. R. T1 - Wide-field Surface Plasmon Resonance Microscopy for in-situ Characterization of Nanoparticle Suspensions T2 - In-situ characterization techniques for nanomaterials Y1 - 2018 SN - 978-3-662-56322-9 SN - 978-3-662-56321-2 U6 - https://doi.org/10.1007/978-3-662-56322-9 SP - 61 EP - 106 PB - Springer Verlag CY - Heidelberg ER - TY - CHAP A1 - Scherbahn, Vitali A1 - Nizamov, Shavkat A1 - Mirsky, Vladimir M. ED - Schöning, Michael J. ED - Poghossian, Arshak T1 - Toward ultrasensitive surface plasmon resonance sensors T2 - Label-free biosensing : advanced materials, devices and applications N2 - Despite the history of application of surface plasmon resonance (SPR) for chemo- and biosensing being over 30 years long, the development of this technique is still in progress. This review is focused on the technological aspects of further improvement of analytical performance of SPR transducers based on Kretschmann configuration. We describe basic measurement configurations, their improvements and optimizations, and their drawbacks and limitations. An importance of referencing in SPR sensors is highlighted. The referencing approaches are classified into the following domains: (1) macroscopic spatially separated referencing, (2) self-referencing based on micro-patterning, (3) in-place referencing, (4) spatiotemporal referencing, and (5) electrochemically assisted referencing. The underlying principles of these approaches, examples of their implementation, and resulting improvements of sensor performance are described. Finally, an analysis of SPR data and an extraction of affinity properties are discussed. KW - Self-referencing KW - Surface plasmon resonance KW - Surface plasmon resonance microscopy Y1 - 2018 SN - 978-3-319-75220-4 SN - 978-3-319-75219-8 U6 - https://doi.org/https://doi.org/10.1007/5346_2017_21 SP - 409 EP - 448 PB - Springer CY - Cham ER - TY - GEN A1 - Tonder, Karin A1 - Nizamov, Shavkat A1 - Scherbahn, Vitali A1 - Mirsky, Vladimir M. T1 - Das Unsichtbare im Blick Ultrasensitive Detektion, Quantifizierung und Identifizierung industriell erzeugter und biologischer Nanopartikel T2 - Laborpraxis Y1 - 2018 UR - https://files.vogel.de/vogelonline/vogelonline/issues/lp/8263.pdf SN - 0344-1733 VL - 42 IS - 1 SP - 24 EP - 26 ER - TY - GEN A1 - Laurinavichyute, Veronika K. A1 - Nizamov, Shavkat A1 - Mirsky, Vladimir M. T1 - Real time tracking of the early stage of electrochemical nucleation T2 - Electrochimica Acta N2 - An analysis of early stage of electrochemical nucleation is crucial in order to understand the mechanism of this process and for electrochemical synthesis of new materials. We show here an application of the wide field surface plasmon microscopy (WF-SPRM) for this purpose. It allows us in-situ monitoring of the growth of nuclei in the range from ~20 till over 1000 nm. The system registers the moment and the place of the formation of each individual nucleus for the total number of the tracked nuclei up to ~10,000 per 1 mm2. The results demonstrate that the integral information obtained from electrochemical measurements can be extracted quantitatively from optical measurements while optical data provide also information on the size of each individual nucleus, individual nucleation time, localization and growth kinetics, and allows one to distinguish the rate limiting stage for each individual nucleus. In this work WF-SPRM was applied for investigation of electrochemical deposition of copper on gold, but this technique can be extended easily to other materials or other nucleation types. KW - Surface plasmon resonance microscopy KW - Nucleation and growth KW - Copper deposition KW - Growth kinetics KW - Diffusion zones Y1 - 2021 U6 - https://doi.org/10.1016/j.electacta.2021.138278 SN - 0013-4686 VL - 382 ER - TY - CHAP A1 - Nizamov, Shavkat A1 - Scherbahn, Vitali A1 - Mirsky, Vladimir M. T1 - Advanced wide-field surface plasmon microscopy of single adsorbing nanoparticles T2 - Optical Sensors 2017, 24–27 April 2017 Prague, Czech Republic N2 - In-situ detection and characterization of nanoparticles in biological media as well as in food or other complex samples is still a big challenge for existing analytical methods. Here we describe a label-free and cost-effective analytical method for detection of nanoparticles in the concentration range 106 -1010 NPs/ml. The proposed method is based on the surface plasmon resonance microscopy (SPRM) with a large field of view (~1.3mm2 ). It is able to detect and count adsorbing nanoparticles individually, totally up to the hundreds of thousands of NPs on the sensor surface. At constant diffusion conditions the detection rate is proportional to the number concentration of NPs, this provides an approach to determine the NPs concentration. The adsorption of nanoparticle can be manipulated by the surface functionalization, pH and electrolyte concentration of suspensions. Images of detected nanoparticles can be quantified in order to characterize them individually. The image intensity grows quasi-linearly with nanoparticle size for the given material. However, the size and material of nanoparticle cannot be resolved directly from the image. For determination of chemical composition, SPRM can be assisted by electrochemical analysis. In this case, the gold sensor surface is used both as a resonant media for plasmon microscopy and as a working electrode. Under potential sweep, the adsorbed NPs can be subjected to electrochemical dissolution, which is detected optically. The potential of this conversion characterizes the material of NPs. Y1 - 2017 SN - 978-1-5106-0963-1 SN - 978-1-5106-0964-8 U6 - https://doi.org/10.1117/12.2267144 PB - SPIE CY - Bellingham, Washington ER - TY - CHAP A1 - Nizamov, Shavkat A1 - Scherbahn, Vitali A1 - Mirsky, Vladimir M. T1 - Wide-field surface plasmon microscopy of nano- and microparticles: features, benchmarking, limitations, and bioanalytical applications T2 - Optical Sensors 2017, 24-27 April 2017, Prague, Czech Republic N2 - Detection of nano- and micro-particles is an important task for chemical analytics, food industry, biotechnology, environmental monitoring and many other fields of science and industry. For this purpose, a method based on the detection and analysis of minute signals in surface plasmon resonance images due to adsorption of single nanopartciles was developed. This new technology allows one a real-time detection of interaction of single nano- and micro-particles with sensor surface. Adsorption of each nanoparticle leads to characteristic diffraction image whose intensity depends on the size and chemical composition of the particle. The adsorption rate characterizes volume concentration of nano- and micro-particles. Large monitored surface area of sensor enables a high dynamic range of counting and to a correspondingly high dynamic range in concentration scale. Depending on the type of particles and experimental conditions, the detection limit for aqueous samples can be below 1000 particles per microliter. For application of method in complex media, nanoparticle images are discriminated from image perturbations due to matrix components. First, the characteristic SPRM images of nanoparticles (templates) are collected in aqueous suspensions or spiked real samples. Then, the detection of nanoparticles in complex media using template matching is performed. The detection of various NPs in consumer products like cosmetics, mineral water, juices, and wines was shown at sub-ppb level. The method can be applied for ultrasensitive detection and analysis of nano- and micro-particles of biological (bacteria, viruses, endosomes), biotechnological (liposomes, protein nanoparticles for drug delivery) or technical origin. Y1 - 2017 SN - 978-1-5106-0963-1 SN - 978-1-5106-0964-8 U6 - https://doi.org/10.1117/12.2267158 PB - SPIE CY - Bellingham, Washington, USA ER - TY - GEN A1 - Nizamov, Shavkat A1 - Dimchevska Sazdovska, Simona A1 - Mirsky, Vladimir M. T1 - A review of optical methods for ultrasensitive detection and characterization of nanoparticles in liquid media with a focus on the wide field surface plasmon microscopy T2 - Analytica Chimica Acta N2 - Development of nanotechnology and corresponding industries during the last decade resulted in a new challenge for analytical science. This includes an ultrasensitive detection and characterization of nanoparticles of different origin and other nanomaterials in various media, including so complex ones as food, biological or environmental samples. The goal of this review is a systematic analysis of possible approaches and description of physical principles behind these methods. The main attention is paid to optical methods which are considered by authors to be mostly effective for the formulated task. Different approaches for detection and analysis of nanoparticles in a volume as well as of those adsorbed on a surface are discussed. While the technologies based on direct analysis of nanoparticle suspensions belong to the established approaches whose development potential has been in large extent exhausted, the novel technologies based on the surface sensing of adsorbed nanoparticles demonstrate intensive development. Therefore, the final part of the review is focused on the wide-field surface plasmon resonance microscopy. It allows one an ultrasensitive detection and characterization of individual nanoparticles of different origin in complex media and provides numerous possibilities for subsequent chemical identification of the detected particles using a hyphenation with other analytical technologies. KW - Nanoparticles KW - Detection of nanoparticles KW - Characterization of nanoparticles KW - Optical techniques KW - Surface plasmon resonance microscopy KW - Nanoparticle tracking analysis Y1 - 2022 U6 - https://doi.org/10.1016/j.aca.2022.339633 SN - 1873-4324 VL - 1204 ER - TY - GEN A1 - Nizamov, Shavkat A1 - Scherbahn, Vitali A1 - Mirsky, Vladimir M. T1 - Detection of Single Sub-Micrometer Objects of Biological or Technical Origin Using Wide Field Surface Plasmon Microscopy T2 - MDPI Proceedings (Journal), Proceedings of the 5th International Symposium on Sensor Science (I3S 2017)) N2 - Detection of nano- and microparticles is an important task for chemical analytics, medical diagnostics, food industry, biotechnology, environmental monitoring and many other fields of science and industry. For this purpose, a method based on the detection and analysis of minute signals of surface plasmon resonance images due to adsorption of single particles was developed. The new technology allows one a real-time detection of interaction of single nano- and microparticles of different origin with sensor surface. Adsorption of each nanoparticle leads to a characteristic diffraction image whose intensity depends on the size and chemical composition of the particle. The number of the nanoparticle - surface binding events per time and surface area characterizes volume concentration. A large monitored surface area of the sensor surface allows one to detect many hundreds events in each frame or totally up to a million particles on the sensor surface, this leads to a very high dynamic range of counting and to a correspondingly high dynamic range in the concentration scale. Depending on the type of particles and experimental conditions, the detection limit for aqueous samples can be below 1000 nanoparticles per microliter. Stable analysis of nanoparticles in very complex environment (fruit juices, wines, cosmetic formulations) was demonstrated. For determination of chemical composition of single nanoparticles separately, the wide field surface plasmon microscopy can be used as a tandem technique. For example, for analysis of technical nanoparticles it can be assisted by electrochemical analysis. In this case, the gold sensor surface is used both for plasmon microscopy and as a working electrode of electrochemical cell. Applying a linear potential sweep to this electrode, adsorbed nanoparticles can be subjected to an electrochemical conversion leading to the change of their refractive index; the value of electrical potential of this conversion characterizes material of the particular nanoparticle. Notably, such analysis is performed simultaneously but independently for each adsorbed particle. The method of wide filed surface plasmon microscopy can be applied for ultrasensitive detection and analysis of nano- and microparticles of biological (bacteria, viruses, exosomes), biotechnological, (liposomes, protein nanoparticles for drug delivery) or technical (metallic, oxides, plastic, etc.) origin. Y1 - 2017 UR - https://www.mdpi.com/2504-3900/1/8/788 U6 - https://doi.org/10.3390/proceedings1080788 VL - 1 IS - 8 PB - MDPI CY - Basel, Switzerland ER -