<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>18142</id>
    <completedYear/>
    <publishedYear>2006</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1</pageFirst>
    <pageLast>6</pageLast>
    <pageNumber/>
    <edition/>
    <issue>1</issue>
    <volume>68</volume>
    <type>articler</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>2017-01-25</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Electrocatalytic activity of DNA on electrodes as an indication of hybridization</title>
    <abstract language="eng">Electron transfer between metal electrodes and ferro/ferricyanide is completely suppressed at low ionic concentration. We describe here a new phenomenon related to this reaction: an immobilisation of thiolated single-stranded DNA on gold electrodes retains this activity at low ionic strength up to the level corresponding to the high ionic strength. In contrast, a hybridisation of the complementary DNAwith the thiolated single-stranded DNA followed by a binding onto the electrodes, attenuated the electrocatalytic effect. These effects can be used for discrimination between single-stranded DNA and double-stranded DNA and for semi-quantitative measurement of complementary DNA in a sample.</abstract>
    <parentTitle language="eng">Bioelectrochemistry</parentTitle>
    <identifier type="doi">10.1016/j.bioelechem.2005.02.006</identifier>
    <identifier type="issn">1567-5394</identifier>
    <enrichment key="BTU">nicht an der BTU erstellt / not created at BTU</enrichment>
    <author>
      <firstName>Zheng-liang</firstName>
      <lastName>Zhi</lastName>
    </author>
    <submitter>
      <firstName>Vladimir M.</firstName>
      <lastName>Mirsky</lastName>
    </submitter>
    <author>
      <firstName>Viktor</firstName>
      <lastName>Drazan</lastName>
    </author>
    <author>
      <firstName>Otto S.</firstName>
      <lastName>Wolfbeis</lastName>
    </author>
    <author>
      <firstName>Vladimir M.</firstName>
      <lastName>Mirsky</lastName>
    </author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>DNA</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>DNA-sensor</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Biosensor</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Electrocatalysi</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Hybridisation</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Oligonucleotide</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Gene analysis</value>
    </subject>
    <collection role="institutes" number="2108">FG Nanobiotechnologie</collection>
  </doc>
</export-example>
