<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>17824</id>
    <completedYear/>
    <publishedYear>2008</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>145</pageFirst>
    <pageLast>149</pageLast>
    <pageNumber/>
    <edition/>
    <issue>1</issue>
    <volume>64</volume>
    <type>articler</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>2016-12-22</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Anomalous adsorptive properties of HIV protease: Indication of two-dimensional crystallization?</title>
    <abstract language="eng">Adsorption of HIV protease onto surfaces that are usually considered to be protein-resistant was studied quantitatively using surface plasmon resonance. Adsorption onto gold surfaces functionalized by OH-terminated alkyl chains was much stronger than onto oligo(ethylene glycol)-terminated surfaces. Equilibrium and kinetic adsorption constants were determined. An anomalous mutual attraction between adsorbate molecules was observed, indicating the possibility of two-dimensional crystallization of HIV protease. These results are applicable for the design of sensors/biosensors for HIV protease resistance detection and for proper manipulation of this enzyme in laboratory devices.</abstract>
    <parentTitle language="eng">Colloids and Surfaces B: Biointerfaces</parentTitle>
    <identifier type="doi">10.1016/j.colsurfb.2008.01.011</identifier>
    <identifier type="issn">0927-7765</identifier>
    <enrichment key="BTU">nicht an der BTU erstellt / not created at BTU</enrichment>
    <author>
      <firstName>Petr</firstName>
      <lastName>Cígler</lastName>
    </author>
    <submitter>
      <firstName>Vladimir M.</firstName>
      <lastName>Mirsky</lastName>
    </submitter>
    <author>
      <firstName>Vladimír</firstName>
      <lastName>Král</lastName>
    </author>
    <author>
      <firstName>Milan</firstName>
      <lastName>Kožíšek</lastName>
    </author>
    <author>
      <firstName>Jan</firstName>
      <lastName>Konvalinka</lastName>
    </author>
    <author>
      <firstName>Vladimir M.</firstName>
      <lastName>Mirsky</lastName>
    </author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>HIV protease</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Protein adsorption</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Protein-resistant surfaces</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Self-assembled monolayer</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Surface plasmon resonance (SPR)</value>
    </subject>
    <collection role="institutes" number="2108">FG Nanobiotechnologie</collection>
  </doc>
</export-example>
