<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>22493</id>
    <completedYear/>
    <publishedYear>2014</publishedYear>
    <thesisYearAccepted/>
    <language>deu</language>
    <pageFirst>309</pageFirst>
    <pageLast>317</pageLast>
    <pageNumber/>
    <edition/>
    <issue>6</issue>
    <volume>38</volume>
    <type>articler</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>2018-11-08</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="deu">Der CytoBead-Assay – Eine neue Möglichkeit der multiparametrischen Autoantikörperanalytik bei systemischen Autoimmunerkrankungen</title>
    <parentTitle language="deu">Journal of Laboratory Medicine</parentTitle>
    <identifier type="doi">10.1515/labmed-2014-0041</identifier>
    <enrichment key="BTU">an der BTU erstellt / created at BTU</enrichment>
    <author>
      <firstName>Mandy</firstName>
      <lastName>Sowa</lastName>
    </author>
    <submitter>
      <firstName>Peter</firstName>
      <lastName>Schierack</lastName>
    </submitter>
    <author>
      <firstName>Kai</firstName>
      <lastName>Großmann</lastName>
    </author>
    <author>
      <firstName>Juliane</firstName>
      <lastName>Scholz</lastName>
    </author>
    <author>
      <firstName>Nadja</firstName>
      <lastName>Röber</lastName>
    </author>
    <author>
      <firstName>Stefan</firstName>
      <lastName>Rödiger</lastName>
    </author>
    <author>
      <firstName>Peter</firstName>
      <lastName>Schierack</lastName>
    </author>
    <author>
      <firstName>Karsten</firstName>
      <lastName>Conrad</lastName>
    </author>
    <author>
      <firstName>Dirk</firstName>
      <lastName>Roggenbuck</lastName>
    </author>
    <author>
      <firstName>Rico</firstName>
      <lastName>Hiemann</lastName>
    </author>
    <collection role="institutes" number="2109">FG Multiparameterdiagnostik</collection>
  </doc>
  <doc>
    <id>22507</id>
    <completedYear/>
    <publishedYear>2015</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1991</pageFirst>
    <pageLast>2002</pageLast>
    <pageNumber/>
    <edition/>
    <issue>12</issue>
    <volume>53</volume>
    <type>articler</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>2018-11-09</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Second generation analysis of antinuclear antibody (ANA) by combination of screening and confirmatory testing</title>
    <parentTitle language="eng">Clinical Chemistry and Laboratory Medicine</parentTitle>
    <identifier type="doi">10.1515/cclm-2015-0083</identifier>
    <identifier type="issn">1437-4331</identifier>
    <enrichment key="BTU">an der BTU erstellt / created at BTU</enrichment>
    <author>
      <firstName>Juliane</firstName>
      <lastName>Scholz</lastName>
    </author>
    <submitter>
      <firstName>Peter</firstName>
      <lastName>Schierack</lastName>
    </submitter>
    <author>
      <firstName>Kai</firstName>
      <lastName>Großmann</lastName>
    </author>
    <author>
      <firstName>Ilka</firstName>
      <lastName>Knütter</lastName>
    </author>
    <author>
      <firstName>Rico</firstName>
      <lastName>Hiemann</lastName>
    </author>
    <author>
      <firstName>Mandy</firstName>
      <lastName>Sowa</lastName>
    </author>
    <author>
      <firstName>Nadja</firstName>
      <lastName>Röber</lastName>
    </author>
    <author>
      <firstName>Stefan</firstName>
      <lastName>Rödiger</lastName>
    </author>
    <author>
      <firstName>Peter</firstName>
      <lastName>Schierack</lastName>
    </author>
    <author>
      <firstName>Dirk</firstName>
      <lastName>Reinhold</lastName>
    </author>
    <author>
      <firstName>Dimitrios Petrou</firstName>
      <lastName>Bogdanos</lastName>
    </author>
    <author>
      <firstName>Pier Luigi</firstName>
      <lastName>Meroni</lastName>
    </author>
    <author>
      <firstName>Antonella</firstName>
      <lastName>Radice</lastName>
    </author>
    <author>
      <firstName>Karsten</firstName>
      <lastName>Conrad</lastName>
    </author>
    <author>
      <firstName>Dirk</firstName>
      <lastName>Roggenbuck</lastName>
    </author>
    <collection role="institutes" number="2109">FG Multiparameterdiagnostik</collection>
  </doc>
  <doc>
    <id>24682</id>
    <completedYear/>
    <publishedYear>2019</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>894</pageFirst>
    <pageLast>904</pageLast>
    <pageNumber/>
    <edition/>
    <issue>7</issue>
    <volume>13</volume>
    <type>articler</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>2019-11-18</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Identification of Chitinase-3-Like Protein 1 as a Novel Neutrophil Antigenic Target in Crohn’s Disease</title>
    <abstract language="eng">Background and Aims&#13;
There is an increasing incidence of inflammatory bowel disease [IBD]. Autoimmune responses are involved in the pathophysiology of IBD, but their underlying pathways and target antigens have not yet been fully elucidated.&#13;
&#13;
Methods&#13;
Autoantigenic targets in IBD were identified after separation of whole cell proteins isolated from neutrophils using two-dimensional electrophoresis and matrix assisted laser desorption ionization – time of flight mass spectrometry-based protein identification of the spots that displayed Western blotting signals with anti-neutrophil cytoplasmic antibody-positive sera. The prevalence of IgG, IgA and secretory IgA [sIgA] to chitinase 3-like protein 1 [CHI3L1] was analysed by enzyme-linked immunosorbent assays using recombinant CHI3L1 in 110 patients with Crohn’s disease [CD], 95 with ulcerative colitis [UC], 126 with coeliac disease [CeD] and 86 healthy controls [HCs].&#13;
&#13;
Results&#13;
The 18-glycosylhydrolase family member CHI3L1 was identified as a neutrophil autoantigenic target. CD patients displayed significantly higher levels of IgG to CHI3L1 than patients with UC and CeD (p &lt; 0.0001, respectively). IgA and sIgA to CHI3L1 was significantly higher in CD than in UC, CeD and HCs [p &lt; 0.0001, respectively]. IgA and sIgA to CHI3L1 demonstrated the highest prevalence in CD [25.5%, 28/110; and 41.8%%, 46/110] compared to HCs [2.3%, 2/86; and 4.7%%, 4/86; p = 0.0015 and p &lt; 0.0001] and are associated with a more complicated progression of CD.&#13;
&#13;
Conclusion&#13;
CHI3L1 is a novel neutrophil autoantigenic target in CD. IgA and sIgA to CHI3L1 may serve as novel markers for CD and may facilitate the serological diagnosis of IBD.</abstract>
    <parentTitle language="eng">Journal of Crohn's and Colitis</parentTitle>
    <identifier type="doi">10.1093/ecco-jcc/jjz012</identifier>
    <identifier type="issn">1876-4479</identifier>
    <identifier type="issn">1873-9946</identifier>
    <enrichment key="BTU">an der BTU erstellt / created at BTU</enrichment>
    <author>
      <firstName>Claudia</firstName>
      <lastName>Deutschmann</lastName>
    </author>
    <submitter>
      <firstName>Claudia</firstName>
      <lastName>Deutschmann</lastName>
    </submitter>
    <author>
      <firstName>Mandy</firstName>
      <lastName>Sowa</lastName>
    </author>
    <author>
      <firstName>Jayaseelan</firstName>
      <lastName>Murugaiyan</lastName>
    </author>
    <author>
      <firstName>Uwe</firstName>
      <lastName>Roessler</lastName>
    </author>
    <author>
      <firstName>Nadja</firstName>
      <lastName>Röber</lastName>
    </author>
    <author>
      <firstName>Karsten</firstName>
      <lastName>Conrad</lastName>
    </author>
    <author>
      <firstName>Martin W.</firstName>
      <lastName>Laass</lastName>
    </author>
    <author>
      <firstName>Dimitrios Petrou</firstName>
      <lastName>Bogdanos</lastName>
    </author>
    <author>
      <firstName>Nora</firstName>
      <lastName>Sipeki</lastName>
    </author>
    <author>
      <firstName>Maria</firstName>
      <lastName>Papp</lastName>
    </author>
    <author>
      <firstName>Stefan</firstName>
      <lastName>Rödiger</lastName>
    </author>
    <author>
      <firstName>Dirk</firstName>
      <lastName>Roggenbuck</lastName>
    </author>
    <author>
      <firstName>Peter</firstName>
      <lastName>Schierack</lastName>
    </author>
    <collection role="institutes" number="2109">FG Multiparameterdiagnostik</collection>
  </doc>
  <doc>
    <id>24684</id>
    <completedYear/>
    <publishedYear>2018</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume/>
    <type>image</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>2019-11-18</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">A novel neutrophil autoantigenic target in inflammatory bowel disease</title>
    <identifier type="url">https://www.researchgate.net/publication/335889899_A_novel_neutrophil_autoantigenic_target_in_inflammatory_bowel_disease</identifier>
    <enrichment key="BTU">an der BTU erstellt / created at BTU</enrichment>
    <enrichment key="opus.doi.autoCreate">false</enrichment>
    <enrichment key="opus.urn.autoCreate">false</enrichment>
    <submitter>
      <firstName>Claudia</firstName>
      <lastName>Deutschmann</lastName>
    </submitter>
    <author>
      <firstName>Mandy</firstName>
      <lastName>Sowa</lastName>
    </author>
    <author>
      <firstName>Jayaseelan</firstName>
      <lastName>Murugaiyan</lastName>
    </author>
    <author>
      <firstName>Karsten</firstName>
      <lastName>Conrad</lastName>
    </author>
    <author>
      <firstName>Martin W.</firstName>
      <lastName>Laass</lastName>
    </author>
    <author>
      <firstName>Dimitrios Petrou</firstName>
      <lastName>Bogdanos</lastName>
    </author>
    <author>
      <firstName>Maria</firstName>
      <lastName>Papp</lastName>
    </author>
    <author>
      <firstName>Stefan</firstName>
      <lastName>Rödiger</lastName>
    </author>
    <author>
      <firstName>Dirk</firstName>
      <lastName>Roggenbuck</lastName>
    </author>
    <author>
      <firstName>Peter</firstName>
      <lastName>Schierack</lastName>
    </author>
    <collection role="institutes" number="2109">FG Multiparameterdiagnostik</collection>
  </doc>
  <doc>
    <id>30677</id>
    <completedYear/>
    <publishedYear>2023</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue>7</issue>
    <volume>24</volume>
    <type>articler</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>2023-03-24</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Over-Expression of LEDGF/p75 in HEp-2 Cells Enhances Autoimmune IgG Response in Patients with Benign Prostatic Hyperplasia—A Novel Diagnostic Approach with Therapeutic Consequence?</title>
    <abstract language="eng">Lens epithelium-derived growth factor splice variant of 75 kDa (LEDGF/p75) is an autoantigen over-expressed in solid tumors and acts as a stress-related transcriptional co-activator. Participation of autoimmune responses in the pathophysiology of benign prostatic hyperplasia (PBH) and a corresponding immunosuppressive therapy by TNFalpha antagonists has been recently suggested. Thus, autoAb testing could aid in the diagnosis of BPH patients profiting from such therapy. We generated CRISPR/Cas9 modified HEp-2 LEDGF knock-out (KO) and HEp-2 LEDGF/p75 over-expressing (OE) cells and examined IgG autoantibody reactivity to LEDGF/p75 in patients with prostate cancer (PCa, n = 89), bladder cancer (BCa, n = 116), benign prostatic hyperplasia (BPH, n = 103), and blood donors (BD, n = 60) by indirect immunofluorescence assay (IFA). Surprisingly, we could not detect elevated binding of autoAbs against LEDGF/p75 in cancer patients, but autoAb reactivity to LEDGF/p75 OE cells in about 50% of patients with BPH was unexpectedly significantly increased. Furthermore, a line immunoassay enabling the detection of 18 different autoAbs revealed a significantly increased occurrence of anti-dsDNA autoAbs in 34% of BPH patients in contrast to tumor patients and BD. This finding was confirmed by anti-mitochondrial (mDNA) autoAb detection with the Crithidia luciliae immunofluorescence test, which also showed a significantly higher prevalence (34%) of anti-mDNA autoAbs in BPH. In summary, our study provided further evidence for the occurrence of autoimmune responses in BPH. Furthermore, LEDGF/p75 over-expression renders HEp-2 cells more autoantigenic and an ideal target for autoAb analysis in BPH with a potential therapy consequence.</abstract>
    <parentTitle language="eng">International Journal of Molecular Sciences</parentTitle>
    <identifier type="issn">1422-0067</identifier>
    <identifier type="doi">10.3390/ijms24076166</identifier>
    <enrichment key="opus.import.date">2023-04-05T16:47:30+00:00</enrichment>
    <enrichment key="opus.source">sword</enrichment>
    <enrichment key="opus.import.user">deepgreen</enrichment>
    <enrichment key="opus.import.file">attachment; filename=deposit.zip</enrichment>
    <enrichment key="opus.import.checksum">8d4acfbddc06b7c30b87da3ba5e692d9</enrichment>
    <enrichment key="BTU">an der BTU erstellt / created at BTU</enrichment>
    <enrichment key="Artikelnummer">6166</enrichment>
    <enrichment key="Publikationsweg">Open Access</enrichment>
    <enrichment key="Fprofil">2 Gesundheit und Lifes Sciences / Health and Life Sciences</enrichment>
    <author>
      <firstName>Victoria</firstName>
      <lastName>Liedtke</lastName>
    </author>
    <author>
      <firstName>Laura</firstName>
      <lastName>Rose</lastName>
    </author>
    <author>
      <firstName>Rico</firstName>
      <lastName>Hiemann</lastName>
    </author>
    <author>
      <firstName>Abdullah</firstName>
      <lastName>Nasser</lastName>
    </author>
    <author>
      <firstName>Stefan</firstName>
      <lastName>Rödiger</lastName>
    </author>
    <author>
      <firstName>Alena</firstName>
      <lastName>Bonaventura</lastName>
    </author>
    <author>
      <firstName>Laura</firstName>
      <lastName>Winkler</lastName>
    </author>
    <author>
      <firstName>Mandy</firstName>
      <lastName>Sowa</lastName>
    </author>
    <author>
      <firstName>Michael</firstName>
      <lastName>Stöckle</lastName>
    </author>
    <author>
      <firstName>Peter</firstName>
      <lastName>Schierack</lastName>
    </author>
    <author>
      <firstName>Kerstin</firstName>
      <lastName>Junker</lastName>
    </author>
    <author>
      <firstName>Dirk</firstName>
      <lastName>Roggenbuck</lastName>
    </author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>LEDGF/p75</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>autoimmunity</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>CRISPR/Cas9</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>dsDNA</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>mDNA</value>
    </subject>
    <collection role="institutes" number="2109">FG Multiparameterdiagnostik</collection>
    <collection role="Import" number="import">Import</collection>
  </doc>
</export-example>
