<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>24835</id>
    <completedYear/>
    <publishedYear>2018</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>81</pageFirst>
    <pageLast>83</pageLast>
    <pageNumber/>
    <edition/>
    <issue>2</issue>
    <volume>3</volume>
    <type>articler</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>2019-12-06</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Actin type and distribution in erythrocytes</title>
    <abstract language="eng">Erythrocytes transport oxygen from the lungs to the tissues. The excess surface area together with the elasticity of the erythrocyte cell membrane provides the flexibility needed to pass through the microvasculature where the oxygen exchange occurs. Although the architecture of the red cell and its membrane-associated cytoskeletal network is known in general, the factors that control the characteristic shape change during echinocyte formation are poorly understood. In this short report we show that in echinocytes a completely reorganized membrane cytoskeleton with a box-like structure of actin filaments prevailed indicating the importance of the actin cytoskeleton during echinocyte formation.</abstract>
    <parentTitle language="eng">Journal of Cellular Biotechnology</parentTitle>
    <identifier type="doi">10.3233/JCB-179014</identifier>
    <identifier type="url">https://content.iospress.com/articles/journal-of-cellular-biotechnology/jcb179014</identifier>
    <identifier type="issn">2352-3697</identifier>
    <identifier type="issn">2352-3689</identifier>
    <enrichment key="BTU">an der BTU erstellt / created at BTU</enrichment>
    <author>
      <firstName>Anne</firstName>
      <lastName>Krüger-Genge</lastName>
    </author>
    <submitter>
      <firstName>Christian</firstName>
      <lastName>Schulz</lastName>
    </submitter>
    <author>
      <firstName>Friedrich</firstName>
      <lastName>Jung</lastName>
    </author>
    <author>
      <firstName>Jan-Heiner</firstName>
      <lastName>Küpper</lastName>
    </author>
    <author>
      <firstName>Christian</firstName>
      <lastName>Lehmann</lastName>
    </author>
    <author>
      <firstName>Ralf-Peter</firstName>
      <lastName>Franke</lastName>
    </author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Erythrocytes</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>echinocytes</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>submembraneous cytoskeleton</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>actin</value>
    </subject>
    <collection role="institutes" number="2110">FG Molekulare Zellbiologie</collection>
  </doc>
  <doc>
    <id>27536</id>
    <completedYear/>
    <publishedYear>2021</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber>15</pageNumber>
    <edition/>
    <issue>3</issue>
    <volume>22</volume>
    <type>articler</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>2021-06-01</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Potential Effects of Nonadherent on Adherent Human Umbilical Venous Endothelial Cells in Cell Culture</title>
    <abstract language="eng">The adherence and shear-resistance of human umbilical venous endothelial cells (HUVEC) on polymers is determined in vitro in order to qualify cardiovascular implant materials. In these tests, variable fractions of HUVEC do not adhere to the material but remain suspended in the culture medium. Nonadherent HUVEC usually stop growing, rapidly lose their viability and can release mediators able to influence the growth and function of the adherent HUVEC. The aim of this study was the investigation of the time dependent behaviour of HUVEC under controlled nonadherent conditions, in order to gain insights into potential influences of these cells on their surrounding environment in particular adherent HUVEC in the context of in vitro biofunctionality assessment of cardiovascular implant materials. Data from adherent or nonadherent HUVEC growing on polystyrene-based cell adhesive tissue culture plates (TCP) or nonadhesive low attachment plates (LAP) allow to calculate the number of mediators released into the culture medium either from adherent or nonadherent cells. Thus, the source of the inflammatory mediators can be identified. For nonadherent HUVEC, a time-dependent aggregation without further proliferation was observed. The rate of apoptotic/dead HUVEC progressively increased over 90% within two days. Concomitant with distinct blebbing and loss of membrane integrity over time, augmented releases of prostacyclin (PGI2, up to 2.91 ± 0.62 fg/cell) and platelet-derived growth factor BB (PDGF-BB, up to 1.46 ± 0.42 fg/cell) were detected. The study revealed that nonadherent, dying HUVEC released mediators, which can influence the surrounding microenvironment and thereby the results of in vitro biofunctionality assessment of cardiovascular implant materials. Neglecting nonadherent HUVEC bears the risk for under- or overestimation of the materials endothelialization potential, which could lead to the loss of relevant candidates or to uncertainty with regard to their suitability for cardiac applications. One approach to minimize the influence from nonadherent endothelial cells could be their removal shortly after observing initial cell adhesion. However, this would require an individual adaptation of the study design, depending on the properties of the biomaterial used.</abstract>
    <parentTitle language="eng">International Journal of Molecular Science</parentTitle>
    <identifier type="url">https://www.mdpi.com/1422-0067/22/3/1493</identifier>
    <identifier type="doi">10.3390/ijms22031493</identifier>
    <enrichment key="BTU">an der BTU erstellt / created at BTU</enrichment>
    <enrichment key="opus.source">publish</enrichment>
    <enrichment key="Fprofil">2 Gesundheit und Lifes Sciences / Health and Life Sciences</enrichment>
    <enrichment key="Fprofil">3 Globaler Wandel und Transformationsprozesse / Global Change and Transformation Processes</enrichment>
    <author>
      <firstName>Christian</firstName>
      <lastName>Schulz</lastName>
    </author>
    <submitter>
      <firstName>Christian</firstName>
      <lastName>Schulz</lastName>
    </submitter>
    <author>
      <firstName>Anne</firstName>
      <lastName>Krüger-Genge</lastName>
    </author>
    <author>
      <firstName>Andreas</firstName>
      <lastName>Lendlein</lastName>
    </author>
    <author>
      <firstName>Jan-Heiner</firstName>
      <lastName>Küpper</lastName>
    </author>
    <author>
      <firstName>Friedrich</firstName>
      <lastName>Jung</lastName>
    </author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>human venous endothelial cells</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>adherent</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>non-adherent</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>viability</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>mediator release</value>
    </subject>
    <collection role="institutes" number="2110">FG Molekulare Zellbiologie</collection>
  </doc>
  <doc>
    <id>32265</id>
    <completedYear/>
    <publishedYear>2023</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue>15</issue>
    <volume>12</volume>
    <type>articler</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>2024-01-04</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Anti-Cancer Prodrug Cyclophosphamide Exerts Thrombogenic Effects on Human Venous Endothelial Cells Independent of CYP450 Activation—Relevance to Thrombosis</title>
    <abstract language="eng">Cancer patients are at a very high risk of serious thrombotic events, often fatal. The causes discussed include the detachment of thrombogenic particles from tumor cells or the adverse effects of chemotherapeutic agents. Cytostatic agents can either act directly on their targets or, in the case of a prodrug approach, require metabolization for their action. Cyclophosphamide (CPA) is a widely used cytostatic drug that requires prodrug activation by cytochrome P450 enzymes (CYP) in the liver. We hypothesize that CPA could induce thrombosis in one of the following ways: (1) damage to endothelial cells (EC) after intra-endothelial metabolization; or (2) direct damage to EC without prior metabolization. In order to investigate this hypothesis, endothelial cells (HUVEC) were treated with CPA in clinically relevant concentrations for up to 8 days. HUVECs were chosen as a model representing the first place of action after intravenous CPA administration. No expression of CYP2B6, CYP3A4, CYP2C9 and CYP2C19 was found in HUVEC, but a weak expression of CYP2C18 was observed. CPA treatment of HUVEC induced DNA damage and a reduced formation of an EC monolayer and caused an increased release of prostacyclin (PGI2) and thromboxane (TXA) associated with a shift of the PGI2/TXA balance to a prothrombotic state. In an in vivo scenario, such processes would promote the risk of thrombus formation.</abstract>
    <parentTitle language="eng">Cells</parentTitle>
    <identifier type="url">https://www.mdpi.com/2073-4409/12/15/1965</identifier>
    <identifier type="doi">10.3390/cells12151965</identifier>
    <identifier type="issn">2073-4409</identifier>
    <enrichment key="BTU">an der BTU erstellt / created at BTU</enrichment>
    <enrichment key="Artikelnummer">1965</enrichment>
    <enrichment key="Publikationsweg">Open Access</enrichment>
    <enrichment key="opus.source">publish</enrichment>
    <enrichment key="Fprofil">2 Gesundheit und Lifes Sciences / Health and Life Sciences</enrichment>
    <enrichment key="Fprofil">3 Globaler Wandel und Transformationsprozesse / Global Change and Transformation Processes</enrichment>
    <author>
      <firstName>Anne</firstName>
      <lastName>Krüger-Genge</lastName>
    </author>
    <submitter>
      <firstName>Christian</firstName>
      <lastName>Schulz</lastName>
    </submitter>
    <author>
      <firstName>Susanne</firstName>
      <lastName>Köhler</lastName>
    </author>
    <author>
      <firstName>Markus</firstName>
      <lastName>Laube</lastName>
    </author>
    <author>
      <firstName>Vanessa</firstName>
      <lastName>Haileka</lastName>
    </author>
    <author>
      <firstName>Sandy</firstName>
      <lastName>Lemm</lastName>
    </author>
    <author>
      <firstName>Karolina</firstName>
      <lastName>Majchrzak</lastName>
    </author>
    <author>
      <firstName>Sarah</firstName>
      <lastName>Kammerer</lastName>
    </author>
    <author>
      <firstName>Christian</firstName>
      <lastName>Schulz</lastName>
    </author>
    <author>
      <firstName>Joachim</firstName>
      <lastName>Storsberg</lastName>
    </author>
    <author>
      <firstName>Jens</firstName>
      <lastName>Pietzsch</lastName>
    </author>
    <author>
      <firstName>Jan-Heiner</firstName>
      <lastName>Küpper</lastName>
    </author>
    <author>
      <firstName>Friedrich</firstName>
      <lastName>Jung</lastName>
    </author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>cancer</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>cyclophosphamide</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>human umbilical vein endothelial cells</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>HUVEC</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>liver</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>cytochrome P450 enzymes (CYP)</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>thrombosis</value>
    </subject>
    <collection role="institutes" number="2110">FG Molekulare Zellbiologie</collection>
  </doc>
</export-example>
