<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>18539</id>
    <completedYear/>
    <publishedYear>1998</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>423</pageFirst>
    <pageLast>426</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>287-288</volume>
    <type>articler</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>2017-02-09</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Thin film electrodes for capacitive chemo- and biosensors: an optimization of the electrodes geometry</title>
    <parentTitle language="eng">Materials Science Forum</parentTitle>
    <identifier type="doi">10.4028/www.scientific.net/MSF.287-288.423</identifier>
    <identifier type="issn">1662-9752</identifier>
    <enrichment key="BTU">nicht an der BTU erstellt / not created at BTU</enrichment>
    <author>
      <firstName>Vladimir M.</firstName>
      <lastName>Mirsky</lastName>
    </author>
    <submitter>
      <firstName>Vladimir M.</firstName>
      <lastName>Mirsky</lastName>
    </submitter>
    <author>
      <firstName>Michael</firstName>
      <lastName>Riepl</lastName>
    </author>
    <author>
      <firstName>Christian</firstName>
      <lastName>Krause</lastName>
    </author>
    <author>
      <firstName>Ivan</firstName>
      <lastName>Novotny</lastName>
    </author>
    <author>
      <firstName>Markus</firstName>
      <lastName>Splonskowski</lastName>
    </author>
    <author>
      <firstName>Vlastimil</firstName>
      <lastName>Rehacek</lastName>
    </author>
    <author>
      <firstName>Vladimir</firstName>
      <lastName>Tvarozek</lastName>
    </author>
    <author>
      <firstName>Helmut</firstName>
      <lastName>Hummel</lastName>
    </author>
    <author>
      <firstName>Otto S.</firstName>
      <lastName>Wolfbeis</lastName>
    </author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Biosensor</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Capacitance</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Chemical Sensors</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Electrode</value>
    </subject>
    <collection role="institutes" number="2108">FG Nanobiotechnologie</collection>
  </doc>
  <doc>
    <id>18546</id>
    <completedYear/>
    <publishedYear>1998</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>3674</pageFirst>
    <pageLast>3678</pageLast>
    <pageNumber/>
    <edition/>
    <issue>17</issue>
    <volume>70</volume>
    <type>articler</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>2017-02-09</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Capacitive approach to determine phospholipase A2 activity towards artificial and natural substrates</title>
    <abstract language="eng">A capacitive approach has been employed to develop a novel method to determine phospholipase activity. The sensing electrodes have a structure like Au/S(CH₂)₁₇CH₃/substrate/electrolyte. Hydrolysis of the substrate, mediated by phospholipase A₂, leads to the formation of water-soluble products from the insoluble substrate. This results in desorption of these products into aqueous phase and corresponding increase of the electrode capacitance. The requirement of high water solubility of the reaction products can be achieved in two ways. In the first, short-chain phospholipids are used as the substrate, in which case, water-soluble products are formed and no additional reagents are required to promote desorption of these products. The sensors prepared by this strategy provide sensitive qualitative detection of phospholipases. The second way is based on the use of a water-soluble acceptor (for example, β-cyclodextrin) to solubilize the products of hydrolysis. It allows semiquantitative detection of phospholipase activity toward long-chain natural substrates. The reaction kinetics for this case was found to be monoexponential and linearly dependent on the phospholipase concentration. The detection limit of this method, as tested with phospholipase A₂ from bee venom and soy bean lecithin as the substrate, is ∼0.5 ng/mL (500 μunits/mL).</abstract>
    <parentTitle language="eng">Analytical Chemistry</parentTitle>
    <identifier type="doi">10.1021/ac980102w</identifier>
    <identifier type="issn">0003-2700</identifier>
    <identifier type="issn">1520-6882</identifier>
    <enrichment key="BTU">nicht an der BTU erstellt / not created at BTU</enrichment>
    <author>
      <firstName>Vladimir M.</firstName>
      <lastName>Mirsky</lastName>
    </author>
    <submitter>
      <firstName>Vladimir M.</firstName>
      <lastName>Mirsky</lastName>
    </submitter>
    <author>
      <firstName>Markus</firstName>
      <lastName>Mass</lastName>
    </author>
    <author>
      <firstName>Christian</firstName>
      <lastName>Krause</lastName>
    </author>
    <author>
      <firstName>Otto S.</firstName>
      <lastName>Wolfbeis</lastName>
    </author>
    <collection role="institutes" number="2108">FG Nanobiotechnologie</collection>
  </doc>
  <doc>
    <id>18549</id>
    <completedYear/>
    <publishedYear>1996</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>179</pageFirst>
    <pageLast>194</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>25</volume>
    <type>articler</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>2017-02-09</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Capacitive detection of adsorption/desorption on hydrophobized gold electrodes: an application for surfactants adsorption and phospholipase assay</title>
    <parentTitle language="eng">Chimica Chronica, New Series</parentTitle>
    <enrichment key="BTU">nicht an der BTU erstellt / not created at BTU</enrichment>
    <author>
      <firstName>Vladimir M.</firstName>
      <lastName>Mirsky</lastName>
    </author>
    <submitter>
      <firstName>Vladimir M.</firstName>
      <lastName>Mirsky</lastName>
    </submitter>
    <author>
      <firstName>Christian</firstName>
      <lastName>Krause</lastName>
    </author>
    <author>
      <firstName>Klaus Dietrich</firstName>
      <lastName>Heckmann</lastName>
    </author>
    <collection role="institutes" number="2108">FG Nanobiotechnologie</collection>
  </doc>
  <doc>
    <id>18550</id>
    <completedYear/>
    <publishedYear>1996</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>6059</pageFirst>
    <pageLast>6064</pageLast>
    <pageNumber/>
    <edition/>
    <issue>25</issue>
    <volume>12</volume>
    <type>articler</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>2017-02-09</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Capacitive detection of surfactant adsorption on hydrophobized gold electrodes</title>
    <abstract language="eng">Nonspecific adsorption of surfactants on polycrystalline gold electrodes, covered by a chemically adsorbed monomolecular layer of octadecanethiol, was studied by means of capacitive measurements. Homologous series of fatty acids (from decane to tetradecane acids) and dodecanepyridinium chloride were used as surfactants. The behavior of the electrode was completely different in the first and subsequent cycles of adsorption/desorption. In the first adsorption cycle, an initial capacitance jump at low concentrations (&lt;10 μM) occurs, while later the curves show the usual saturation behavior. The first surfactant layer could not be completely desorbed; only the electrode capacitance shown after the jump was reached. Subsequent cycles do not reveal any capacitance jumps and were completely reversible. Adsorption curves in the series of monoalkyl acids are shifted according to the Traube rule. A comparison with the surfactant adsorption at the air/water interface has been done.</abstract>
    <parentTitle language="eng">Langmuir</parentTitle>
    <identifier type="doi">10.1021/la9601869</identifier>
    <identifier type="issn">0743-7463</identifier>
    <enrichment key="BTU">nicht an der BTU erstellt / not created at BTU</enrichment>
    <author>
      <firstName>Christian</firstName>
      <lastName>Krause</lastName>
    </author>
    <submitter>
      <firstName>Vladimir M.</firstName>
      <lastName>Mirsky</lastName>
    </submitter>
    <author>
      <firstName>Vladimir M.</firstName>
      <lastName>Mirsky</lastName>
    </author>
    <author>
      <firstName>Klaus Dietrich</firstName>
      <lastName>Heckmann</lastName>
    </author>
    <collection role="institutes" number="2108">FG Nanobiotechnologie</collection>
  </doc>
  <doc>
    <id>18551</id>
    <completedYear/>
    <publishedYear>1996</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>939</pageFirst>
    <pageLast>941</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>284-285</volume>
    <type>articler</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>2017-02-09</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Capacitive sensor for lipolytic enzymes</title>
    <abstract language="eng">The preparation of the capacitive biosensor for monitoring the activity of enzymes (lipases, phospholipases, etc.) hydrolysing waterinsoluble substrates into water-soluble products is described. The sensor is based on a sandwich-like structure: Au/S(CH₂)₁₇CH₁/substrate/electrolyte. Hydrolysis of the substrate leads to the formation of water-soluble products and desorption of these compounds from the electrode. When the product formation is the rate-limiting step of this process, the desorption rate is determined by the enzyme activity. The desorption can be easily monitored as an increase of the electrode capacitance. By following this principle it was possible to develop a sensor monitoring phospholipase A₂ activity even at the 50 pg ml-1 level.</abstract>
    <parentTitle language="eng">Thin Solid Films</parentTitle>
    <identifier type="doi">10.1016/S0040-6090(95)08485-1</identifier>
    <identifier type="issn">0040-6090</identifier>
    <enrichment key="BTU">nicht an der BTU erstellt / not created at BTU</enrichment>
    <author>
      <firstName>Vladimir M.</firstName>
      <lastName>Mirsky</lastName>
    </author>
    <submitter>
      <firstName>Vladimir M.</firstName>
      <lastName>Mirsky</lastName>
    </submitter>
    <author>
      <firstName>Christian</firstName>
      <lastName>Krause</lastName>
    </author>
    <author>
      <firstName>Klaus Dietrich</firstName>
      <lastName>Heckmann</lastName>
    </author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Phospholipase AZ</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Enzyme assay</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Capacitive sensor</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Self-assembly layer</value>
    </subject>
    <collection role="institutes" number="2108">FG Nanobiotechnologie</collection>
  </doc>
</export-example>
