@misc{LehmannMartinMannigeletal., author = {Lehmann, Mario and Martin, Frank and Mannigel, Kerstin and Kaltschmidt, Kristin and Sack, Ulrich and Anderer, Ursula}, title = {Three-dimensional scaffold-free fusion culture: the way to enhanced chondrogenesis of in vitro propagated human articular chondrocytes}, series = {European Journal of Histochemistry}, volume = {57}, journal = {European Journal of Histochemistry}, number = {4}, issn = {2038-8306}, doi = {10.4081/ejh.2013.e31}, pages = {205 -- 215}, language = {en} } @misc{MartinLehmannSchlaegeretal., author = {Martin, Frank and Lehmann, Mario and Schl{\"a}ger, Peter and Sack, Ulrich and Anderer, Ursula}, title = {Differentiation capacity of chondrocytes in microtissues depends on TGF-ß subtyp}, series = {Journal of biochips \& tissue chips : JBTC}, volume = {S2}, journal = {Journal of biochips \& tissue chips : JBTC}, number = {002}, issn = {2153-0777}, doi = {10.4172/2153-0777.S2-002}, pages = {11}, language = {en} } @misc{KnuetterHiemannBrummaetal., author = {Kn{\"u}tter, Ilka and Hiemann, Rico and Brumma, Therese and B{\"u}ttner, Thomas and Großmann, Kai and Cusini, Marco and Pregnolato, Francesca and Borghi, Maria Orietta and Anderer, Ursula and Conrad, Karsten and Reinhold, Dirk and Roggenbuck, Dirk and Csernok, Elena}, title = {Automated interpretation of ANCA patterns - a new approach in the serology of ANCA-associated vasculitis}, series = {Arthritis Research \& Therapy}, volume = {14}, journal = {Arthritis Research \& Therapy}, issn = {1465-9913}, doi = {10.1186/ar4119}, pages = {10}, language = {en} } @misc{LutterScholkaRichteretal., author = {Lutter, Anne-Helen and Scholka, Jenny and Richter, Heiko and Anderer, Ursula}, title = {Applying XTT, WST-1, and WST-8 to human chondrocytes: A comparison of membrane-impermeable tetrazolium salts in 2D and 3D cultures}, series = {Clinical Hemorheology and Microcirculation}, volume = {67}, journal = {Clinical Hemorheology and Microcirculation}, number = {3-4}, issn = {1875-8622}, doi = {10.3233/CH-179213}, pages = {327 -- 342}, language = {en} } @misc{LutterHempelAndereretal., author = {Lutter, Anne-Helen and Hempel, Ute and Anderer, Ursula and Dieter, Peter}, title = {Biphasic influence of PGE2 on the resorption activity of osteoclast-like cells derived from human peripheral blood monocytes and mouse RAW264.7}, series = {Prostaglandins, Leukotrienes and Essential Fatty Acids}, volume = {111}, journal = {Prostaglandins, Leukotrienes and Essential Fatty Acids}, issn = {1532-2823}, doi = {10.1016/j.plefa.2016.03.017}, pages = {1 -- 7}, language = {en} } @misc{MartinLehmannSacketal., author = {Martin, Frank and Lehmann, Mario and Sack, Ulrich and Anderer, Ursula}, title = {In vitro development of personalized cartilage microtissues uncovers an individualized differentiation capacity of human chondrocytes}, series = {Experimental Biology and Medicine}, volume = {242}, journal = {Experimental Biology and Medicine}, number = {18}, issn = {1535-3702}, doi = {10.1177/1535370217728498}, pages = {1746 -- 1756}, language = {en} } @incollection{AndererSavkovic, author = {Anderer, Ursula and Savkovic, Vuc}, title = {Zelltherapien in der Regenerativen Medizin}, series = {Zellul{\"a}re Diagnostik und Therapie}, booktitle = {Zellul{\"a}re Diagnostik und Therapie}, editor = {Sack, Ulrich}, edition = {1. Auflage}, publisher = {Walter de Gruyter}, address = {Berlin [u.a.]}, isbn = {978-3-11-034406-6}, pages = {291 -- 341}, language = {de} } @incollection{MartinLehmannAnderer, author = {Martin, Frank and Lehmann, Mario and Anderer, Ursula}, title = {Generation of scaffold free 3-D cartilage-like microtissues from human chondrocytes}, series = {Medical Advancements in Aging and Regenerative Technologies: Clinical Tools and Applications}, booktitle = {Medical Advancements in Aging and Regenerative Technologies: Clinical Tools and Applications}, editor = {Daskalaki, Adriani}, publisher = {IGI Global}, address = {Hershey, PA, USA}, isbn = {978-1-4666-2506-8}, pages = {169 -- 194}, language = {en} } @misc{LutterLiedtkeEckeetal., author = {Lutter, Anne-Helen and Liedtke, Victoria and Ecke, Annemarie and Scholka, Jenny and Muschter, Antje and Hentschel, Erik and Becker, Roland and Anderer, Ursula}, title = {Direct co-culture of human chondrogenic microtissues with osteoblast-like Saos-2 cells positively influence the differentiation potential of cartilage cells in 3D culture}, doi = {10.1007\%2Fs10354-018-0664-9.pdf}, pages = {S. 51}, language = {en} } @misc{LutterMuschterAnderer, author = {Lutter, Anne-Helen and Muschter, Antje and Anderer, Ursula}, title = {Diagnostic Test System to detect resorption activity of osteoclasts in vitro just using small blood samples}, series = {Osteologie}, volume = {28}, journal = {Osteologie}, number = {4}, publisher = {Georg Thieme Verlag KG}, address = {Stuttgart ; New York}, issn = {2567-5818}, doi = {10.1055/s-0039-1680038}, pages = {72}, language = {en} } @misc{EckeScholkaHanschetal., author = {Ecke, Annemarie and Scholka, Jenny and Hansch, Anna and Becker, Roland and Anderer, Ursula}, title = {Agar overlay technique vs. v-bottom plate: differentiation of human cartilage-like microtissues depend on plate format.}, series = {Osteologie}, volume = {28}, journal = {Osteologie}, number = {4}, publisher = {Georg Thieme Verlag KG}, address = {Stuttgart ; New York}, issn = {2567-5818}, doi = {10.1055/s-0039-1680058}, pages = {81}, language = {en} } @misc{LutterEckeScholkaetal., author = {Lutter, Anne-Helen and Ecke, Annemarie and Scholka, Jenny and Waldeck, Peter and Tretter, Tim and Anderer, Ursula}, title = {Differentiation of human chondrocytes under the influence of microalgae-based exopolysaccharides}, series = {38. Jahrestagung der Deutschen Gesellschaft f{\"u}r Klinische Mikrozirkulation und H{\"a}morheologie : Programm, 22. und 23. November 2019, Braunschweig}, journal = {38. Jahrestagung der Deutschen Gesellschaft f{\"u}r Klinische Mikrozirkulation und H{\"a}morheologie : Programm, 22. und 23. November 2019, Braunschweig}, abstract = {BACKGROUND: Exopolysaccharides are formed by microalgae in response to adverse conditions and are found mainly as a coating around the cells. The protective function of EPS plays an important role in the survival of microalgae in polluted habitats. So far, the particular biological and physico-chemical properties of EPS have not been adequately investigated and their effect on cells such as human chondrocytes has not been tested. Microalgae in particular offer an ideal platform for the production of dietary supplements or drugs that enable new therapeutic approaches. METHODS: To investigate the influence of EPS on human chondrocytes, cytotoxicity and differentiation potential were analyzed in 3D cultures. RESULTS: We were able to demonstrate a positive, but donor-specific influence of EPS on the differentiation of human chondrocytes. Furthermore, the EPS showed no cytotoxic effects in a range of up to 250 µg EPS per ml medium. CONCLUSION: EPS have the potential to positively influence the differentiation of human chondrocytes. Further experiments are necessary to analyze the donor-specific effect.}, language = {en} } @misc{EckeScholkaHanschetal., author = {Ecke, Annemarie and Scholka, Jenny and Hansch, Anna and Becker, Roland and Anderer, Ursula}, title = {Differentiation of human articular chondrocytes in microtissues depends on the cell aggregation technique}, pages = {1}, language = {en} } @inproceedings{LehmannMartinScholkaetal., author = {Lehmann, Mario and Martin, Frank and Scholka, Jenny and Petto, Carola and Wittenburg, Gretel and Sack, Ulrich and Anderer, Ursula}, title = {Human chondrocytes and dental pulp stem cells enhance their chondrogenic differentiation potential via a two-step fusion culture technique}, series = {Tissue Engineering, Part A, Vol. 21, Suppl. 1}, booktitle = {Tissue Engineering, Part A, Vol. 21, Suppl. 1}, doi = {10.1089/ten.tea.2015.5000.abstracts}, pages = {S-268 -- S-269}, language = {en} } @inproceedings{LutterHempelDieteretal., author = {Lutter, Anne-Helen and Hempel, Ute and Dieter, Peter and Anderer, Ursula}, title = {Cost-efficient diagnostic platform to measure osteoclast activity}, series = {35. Jahrestagung der Deutschen Gesellschaft f{\"u}r Klinische Mikrozirkulation und H{\"a}morheologie, Mainz, 2016}, booktitle = {35. Jahrestagung der Deutschen Gesellschaft f{\"u}r Klinische Mikrozirkulation und H{\"a}morheologie, Mainz, 2016}, doi = {10.13140/RG.2.2.10550.19528}, language = {en} } @inproceedings{LehmannVossMartinetal., author = {Lehmann, Mario and Voß, Fr{\"a}nzi and Martin, Frank and Schl{\"a}ger, Peter and Anderer, Ursula}, title = {Chondrogenic microtissues in the lab \& for the clinic}, series = {3D cell culture 2016, book of abstracts, DECHEMA Biotechnologie}, booktitle = {3D cell culture 2016, book of abstracts, DECHEMA Biotechnologie}, publisher = {DECHEMA e.V.}, address = {Frankfurt am Main}, isbn = {978-3-89746-182-6}, pages = {52 -- 53}, language = {en} } @inproceedings{LehmannVossMartinetal., author = {Lehmann, Mario and Voß, Fr{\"a}nzi and Martin, Frank and Richter, Heiko and Anderer, Ursula}, title = {Chondrogenic microtissues with a lower differentiation degree used as in vitro transplants show improved integration in the tissue repair process in an in vitro therapy culture system}, series = {Regenerative Medicine}, volume = {10}, booktitle = {Regenerative Medicine}, number = {7S}, issn = {1746-0751}, pages = {S. 53}, language = {en} } @inproceedings{LehmannScholkaWittenburgetal., author = {Lehmann, Mario and Scholka, Jenny and Wittenburg, Gretel and Petto, Carola and Geppert, Philipp and Muschter, Antje and Anderer, Ursula}, title = {An easily accessible adult stem cell pool may be suitable for tissue engineering-based regeneration of articular cartilage defects}, series = {eCM Conferences Open Access online periodical}, booktitle = {eCM Conferences Open Access online periodical}, isbn = {2522-235X}, pages = {P190}, language = {en} } @misc{HuettnerHenzeElsteretal., author = {H{\"u}ttner, S{\"o}ren and Henze, Henriette and Elster, Dana and Koch, Philipp and Anderer, Ursula and Eyss, Bj{\"o}rn von and Maltzahn, Julia von}, title = {A dysfunctional miR-1-TRPS1-MYOG axis drives ERMS by suppressing terminal myogenic differentiation}, series = {Molecular Therapy}, volume = {31}, journal = {Molecular Therapy}, number = {9}, issn = {1525-0024}, doi = {10.1016/j.ymthe.2023.07.003}, pages = {2612 -- 2632}, abstract = {Rhabdomyosarcoma is the most common pediatric soft tissue tumor, comprising two major subtypes: the PAX3/7-FOXO1 fusion-negative embryonal and the PAX3/7-FOXO1 fusion-positive alveolar subtype. Here, we demonstrate that the expression levels of the transcriptional repressor TRPS1 are specifically enhanced in the embryonal subtype, resulting in impaired terminal myogenic differentiation and tumor growth. During normal myogenesis, expression levels of TRPS1 have to decrease to allow myogenic progression, as demonstrated by overexpression of TRPS1 in myoblasts impairing myotube formation. Consequentially, myogenic differentiation in embryonal rhabdomyosarcoma in vitro as well as in vivo can be achieved by reducing TRPS1 levels. Furthermore, we show that TRPS1 levels in RD cells, the bona fide model cell line for embryonal rhabdomyosarcoma, are regulated by miR-1 and that TRPS1 and MYOD1 share common genomic binding sites. The myogenin (MYOG) promoter is one of the critical targets of TRPS1 and MYOD1; we demonstrate that TRPS1 restricts MYOG expression and thereby inhibits terminal myogenic differentiation. Therefore, reduction of TRPS1 levels in embryonal rhabdomyosarcoma might be a therapeutic approach to drive embryonal rhabdomyosarcoma cells into myogenic differentiation, thereby generating postmitotic myotubes.}, language = {en} } @misc{HiemannRoggenbuckSacketal., author = {Hiemann, Rico and Roggenbuck, Dirk and Sack, Ulrich and Anderer, Ursula and Conrad, Karsten}, title = {Die HEp-2-Zelle als Target f{\"u}r multiparametrische Autoantik{\"o}rperanalytik - Automatisierung und Standardisierung/The HEp-2 cell as target for multiparametric autoantibody analyses: automation and standardisation}, series = {Journal of Laboratory Medicine}, volume = {35}, journal = {Journal of Laboratory Medicine}, number = {6}, issn = {1439-0477}, doi = {10.1515/JLM.2011.053}, pages = {351 -- 361}, abstract = {Trotz Einf{\"u}hrung von Multiplex-Assays ist die Bestimmung von Autoantik{\"o}rpern (AAK) mittels indirekter Immunfluoreszenz (IIF) nach wie vor ein wesentlicher Bestandteil der Autoimmundiagnostik. Das Screening auf nichtorganspezifische AAK wird in der Routinediagnostik von autoimmunen rheumatischen Erkrankungen mittels indirekter Immunfluoreszenz an HEp-2-Zellen durchgef{\"u}hrt. Leider differieren die mit diesem Test erhaltenen Befunde z. T. recht erheblich auf Grund von objektiven (z. B. HEp-2-Zellfixierung) und subjektiven (z. B. Erfahrungswerte) Faktoren. Die Intra- und Interlaborvarianzen sind daher relativ hoch. Erste Ergebnisse von Evaluierungsstudien eines neu entwickelten Systems zur automatischen Bildanalyse (AKLIDES®) zeigen einen Weg in Richtung Standardisierung (bzw. Harmonisierung) des HEp-2-Zell-Assays auf, in dem zun{\"a}chst subjektive Fehlinterpretationen vermieden werden k{\"o}nnen. Eine weitere Harmonisierung erfordert die Weiterentwicklung der Software-Algorithmen zur Mustererkennung sowie neue Kalibrationssysteme.}, language = {en} } @misc{HahmAnderer, author = {Hahm, Daniel and Anderer, Ursula}, title = {Establishment of HEp-2 cell preparation for automated analysis of ANA fluorescence pattern}, series = {Cytometry. Part A}, volume = {69A}, journal = {Cytometry. Part A}, number = {3}, issn = {1552-4922}, doi = {10.1002/cyto.a.20223}, pages = {178 -- 181}, abstract = {AbstractBackgroundIdentification of antinuclear antibodies (ANAs) has large clinical importance for the assessment of autoimmune diseases. HEp-2 cell preparations on microscopic slides are commonly used as antigenic substrate. Methods used for cell preparation are important for ANA pattern analysis; however, these methods differ widely and are mostly not specified.MethodsHEp-2 cells were fixed using acetic acid-ethanol, methanol-acetone, acetone, formaldehyde, paraformaldehyde, or glutaraldehyde. Morphological analysis was done after haematoxylin-eosin staining and DAPI-staining of cell nuclei.ResultsThe results demonstrate a high variability of cell and nuclear morphology depending on the used fixatives. Aldehyde fixatives conserved the cell structures best, acetone fixatives revealed remarkable changes.ConclusionsAfter selecting appropriate fixation procedures to preserve nuclear structures further experiments are necessary to find out which fixation procedure preserves the disease-linked antigens the best way and are, therefore, suitable to be used in ANA-testing of AABs. © 2006 International Society for Analytical Cytology}, language = {en} } @misc{HiemannHilgerMicheletal., author = {Hiemann, Rico and Hilger, Nadja and Michel, J{\"o}rg and Nitschke, J{\"o}rg and B{\"o}hm, Alexander and Anderer, Ursula and Weigert, Martin and Sack, Ulrich}, title = {Automatic Analysis of Immunofluorescence Patterns of HEp-2 Cells}, series = {Annals of the New York Academy of Sciences}, volume = {1109}, journal = {Annals of the New York Academy of Sciences}, number = {1}, issn = {0077-8923}, doi = {10.1196/annals.1398.042}, pages = {358 -- 371}, abstract = {The standard screening test for the recognition of autoimmune diseases is the proof of autoantibodies in serum of patients by indirect immunofluorescence (IIF) based on HEp-2 cells. Manual evaluation of this test is very subjective, slow, and there are no objective parameters as guidelines available. Interlaboratory tests showed occasionally large deviations in the test evaluation resulting in a high variance of results. The aim of this project is fast, objective, safe, and economical automatic analysis of HEp-2 IIF patterns. Images of IIF patterns were completely and automatically captured using an inverse motorized fluorescence microscope. Thereby, device-specific parameters were controlled automatically, too. For fast analysis of IIF patterns new algorithms of image processing were developed. Artifacts were recognized and excluded from analysis by the developed software. Analysis of more than 80,000 images clearly demonstrated full automatization and fast processing of IIF patterns. Additionally serum-specific fluorescence could be easily distinguished from background. Even very weak but positive patterns can be recognized and used for diagnosis. A detailed separation into different basic patterns is possible. Objective, fast, and disease-related economical analysis of HEp-2 immunofluorescence patterns is feasible. The implemented software algorithms allowed a mathematical way of describing IIF patterns and can therefore be a useful tool for the needed standardization process.}, language = {en} } @misc{AndererScholkaHanschetal., author = {Anderer, Ursula and Scholka, Jenny and Hansch, Anna and Becker, Roland and Ecke, Annemarie}, title = {Influence of the cell aggregation technique on the differentiation of human articular chondrocytes in microtissues}, series = {Osteologie / Osteology}, volume = {31}, journal = {Osteologie / Osteology}, number = {03}, publisher = {Georg Thieme Verlag}, address = {Stuttgart}, issn = {1019-1291}, doi = {10.1055/s-0042-1755855}, pages = {201 -- 202}, language = {en} } @misc{EckeLutterScholkaetal., author = {Ecke, Annemarie and Lutter, Anne-Helen and Scholka, Jenny and Hansch, Anna and Becker, Roland and Anderer, Ursula}, title = {Tissue Specific Differentiation of Human Chondrocytes Depends on Cell Microenvironment and Serum Selection}, series = {Cells}, volume = {8}, journal = {Cells}, number = {8}, issn = {2073-4409}, doi = {10.3390/cells8080934}, pages = {15}, language = {en} } @misc{EckeScholkaHanschetal., author = {Ecke, Annemarie and Scholka, Jenny and Hansch, Anna and Becker, Roland and Anderer, Ursula}, title = {Human serum or FCS: Differentiation of human cartilage-like microtissues depends on serum selection}, series = {DGZ 2018 International Meeting of the German Society for Cell Biology: "Concepts of Cell Organisation and Dynamics"}, journal = {DGZ 2018 International Meeting of the German Society for Cell Biology: "Concepts of Cell Organisation and Dynamics"}, abstract = {Introduction: Regeneration of articular cartilage defects is limited. Hence, cell-based therapies for the treatment were developed using e.g. chondrocytes isolated from patient biopsies. For cell culture fetal calf serum (FCS) is often used as media supplement to establish engineering processes for transplants in regenerative medicine. However, the usage of xenogeneic serum could mask the intrinsic behavior of human cells in vitro. This could be particularly important for the dedifferentiation process during cell expansion and redifferentiation in 3D-culture. The aim of this study was to compare the development of microtissues derived from chondrocytes cultivated in presence of FCS with those cultivated in human serum (HS). Material and Methods: Human articular chondrocytes isolated from knee joints were expanded in medium with HS or FCS. Scaffold-free microtissues were generated using the agar overlay technique. After four and eight weeks the macroscopic appearance (reflected-light microscopy) was documented and the size of the microtissues was determined. The differentiation degree was evaluated via histology to visualize typical glycosamino¬glycans (Safranin O, Alcian blue) and immunohisto¬chemistry (IH) to detect cartilage-specific markers (collagen type II, proteoglycans, COMP, Sox9) on cryosections. Results and Conclusion: Cells from all donors in both medium compositions generated microtissues - but with distinct differences in the differentiation levels. Microtissues cultured in medium with FCS showed a higher differentiation level compared to microtissues in HS. This is evidenced by a higher amount of matrix resulting in bigger microtissues (diameter in FCS is 42\% larger compared to HS) and a more cartilage-like composition of the matrix (not any/little colouring in HS versus intense staining in FCS). IH analysis showed the same pattern. Not any/less positivity for collagen type II, proteoglycan, COMP, and Sox9 in HS versus moderate-to-high positivity in FCS-cultured microtissues. Absolute values vary from donor to donor whereas an individualized expression profile only became visible in microtissues cultured in HS medium. HS should be preferred for the engineering of cartilage-like microtissues, as it rather enables a "human-based" situation in vitro. Supported by „Gesundheitscampus Brandenburg" and „Ministerium f{\"u}r Wissenschaft, Forschung und Kultur", state of Brandenburg, Germany.}, language = {en} } @misc{LutterLiedtkeScholkaetal., author = {Lutter, Anne-Helen and Liedtke, Victoria and Scholka, Jenny and Muschter, Antje and Becker, Roland and Anderer, Ursula}, title = {Co-culture of human chondrogenic microtissues with osteoblast-like Saos-2 cells or HFF-1 fibroblasts influence the differentiation potential of spheroids}, series = {Journal of Cellular Biotechnology}, volume = {4}, journal = {Journal of Cellular Biotechnology}, number = {1/2}, issn = {2352-3697}, doi = {10.3233/JCB-189005}, pages = {31 -- 42}, abstract = {BACKGROUND: Tissue engineering has become a major field of research in biotechnology and biomedicine. As a consequence, cell-based therapeutic approaches are entering the hospitals, especially for skeletal regeneration. Traumatic injuries of cartilage are treated with autologous cell suspensions or in vitro generated cartilage tissues, but there is actually no therapy available for degenerative cartilage defects. However, Osteoarthritis (OA) is a major public health problem in the world affecting 240 million people globally. OBJECTIVE: To develop suitable in vitro tissues, the properties of chondrogenic spheroids should be optimized via coculture with cells naturally occurring as joint neighbours. METHODS: Human chondrocytes were isolated from condyles and propagated in monolayer culture. Scaffold-free spheroids were generated and co-cultured with joint-specific partner cells (osteoblast-like osteosarcoma cells, fibroblasts). Morphology and differentiation was analyzed using histochemistry (Alcian blue, Safranin O) and immunohistochemistry for cartilage markers (collagen type II, Sox9, proteoglycan), proliferation-associated protein (Ki67) and markers of connective tissue (collagen type I and actin). RESULTS: The provision of a more natural microenvironment in vitro via co-culture of chondrocyte-based aggregates with osteoblast-like Saos-2 cells enhanced the differentiation potential of chondrogenic spheroids towards hyaline cartilage. CONCLUSIONS: The study showed the positive influence of Saos-2 cells on the differentiation potential of human chondrocytes in co-culture.}, language = {en} } @misc{LutterHempelDieteretal., author = {Lutter, Anne-Helen and Hempel, Ute and Dieter, Peter and Anderer, Ursula}, title = {Multinuclearity is not mandatory for Bone Resorption Mononuclear Osteoclasts are able to resorbe Extracellular Bone Matrix in vitro}, series = {Bone-Tec, Munich, 2017}, journal = {Bone-Tec, Munich, 2017}, language = {en} } @misc{LutterHempelDieteretal., author = {Lutter, Anne-Helen and Hempel, Ute and Dieter, Peter and Anderer, Ursula}, title = {The biomarker-based ODEM-assay affects cellular characteristics of osteoclasts in vitro Tissue culture polystyrene vs Osteoblast-Derived Extracellular Matrix}, doi = {10.13140/RG.2.2.20195.96806}, pages = {1}, language = {en} } @misc{LutterScholkaRichteretal., author = {Lutter, Anne-Helen and Scholka, Jenny and Richter, Heiko and Anderer, Ursula}, title = {Comparison of membrane-impermeable tetrazolium salt assays in 2D and 3D cultures of human chondrocytes}, doi = {10.13140/RG.2.2.10682.49600}, language = {en} } @misc{SowaGrossmannKnuetteretal., author = {Sowa, Mandy and Großmann, Kai and Kn{\"u}tter, Ilka and Hiemann, Rico and R{\"o}ber, Nadja and Anderer, Ursula and Csernok, Elena and Bogdanos, Dimitrios Petrou and Borghi, Maria Orietta and Meroni, Pier Luigi and Schierack, Peter and Reinhold, Dirk and Conrad, Karsten and Roggenbuck, Dirk}, title = {Simultaneous automated screening and confirmatory testing for vasculitis-specific ANCA}, series = {PLoS ONE}, volume = {9}, journal = {PLoS ONE}, number = {9}, issn = {1932-6203}, doi = {10.1371/journal.pone.0107743}, pages = {11}, language = {en} } @misc{WillitzkiHiemannPetersetal., author = {Willitzki, Annika and Hiemann, Rico and Peters, Vanessa and Sack, Ulrich and Schierack, Peter and R{\"o}diger, Stefan and Anderer, Ursula and Conrad, Karsten and Bogdanos, Dimitrios Petrou and Reinhold, Dirk and Roggenbuck, Dirk}, title = {New PlatformTechnology for Comprehensive Serological Diagnostics of Autoimmune Diseases}, series = {Clinical and Developmental Immunology}, journal = {Clinical and Developmental Immunology}, issn = {1740-2530}, doi = {10.1155/2012/284740}, pages = {1 -- 8}, language = {en} }