@misc{MartinLehmannSacketal., author = {Martin, Frank and Lehmann, Mario and Sack, Ulrich and Anderer, Ursula}, title = {In vitro development of personalized cartilage microtissues uncovers an individualized differentiation capacity of human chondrocytes}, series = {Experimental Biology and Medicine}, volume = {242}, journal = {Experimental Biology and Medicine}, number = {18}, issn = {1535-3702}, doi = {10.1177/1535370217728498}, pages = {1746 -- 1756}, language = {en} } @incollection{AndererSavkovic, author = {Anderer, Ursula and Savkovic, Vuc}, title = {Zelltherapien in der Regenerativen Medizin}, series = {Zellul{\"a}re Diagnostik und Therapie}, booktitle = {Zellul{\"a}re Diagnostik und Therapie}, editor = {Sack, Ulrich}, edition = {1. Auflage}, publisher = {Walter de Gruyter}, address = {Berlin [u.a.]}, isbn = {978-3-11-034406-6}, pages = {291 -- 341}, language = {de} } @incollection{MartinLehmannAnderer, author = {Martin, Frank and Lehmann, Mario and Anderer, Ursula}, title = {Generation of scaffold free 3-D cartilage-like microtissues from human chondrocytes}, series = {Medical Advancements in Aging and Regenerative Technologies: Clinical Tools and Applications}, booktitle = {Medical Advancements in Aging and Regenerative Technologies: Clinical Tools and Applications}, editor = {Daskalaki, Adriani}, publisher = {IGI Global}, address = {Hershey, PA, USA}, isbn = {978-1-4666-2506-8}, pages = {169 -- 194}, language = {en} } @inproceedings{LehmannScholkaWittenburgetal., author = {Lehmann, Mario and Scholka, Jenny and Wittenburg, Gretel and Petto, Carola and Geppert, Philipp and Muschter, Antje and Anderer, Ursula}, title = {An easily accessible adult stem cell pool may be suitable for tissue engineering-based regeneration of articular cartilage defects}, series = {eCM Conferences Open Access online periodical}, booktitle = {eCM Conferences Open Access online periodical}, isbn = {2522-235X}, pages = {P190}, language = {en} } @misc{SowaGrossmannKnuetteretal., author = {Sowa, Mandy and Großmann, Kai and Kn{\"u}tter, Ilka and Hiemann, Rico and R{\"o}ber, Nadja and Anderer, Ursula and Csernok, Elena and Bogdanos, Dimitrios Petrou and Borghi, Maria Orietta and Meroni, Pier Luigi and Schierack, Peter and Reinhold, Dirk and Conrad, Karsten and Roggenbuck, Dirk}, title = {Simultaneous automated screening and confirmatory testing for vasculitis-specific ANCA}, series = {PLoS ONE}, volume = {9}, journal = {PLoS ONE}, number = {9}, issn = {1932-6203}, doi = {10.1371/journal.pone.0107743}, pages = {11}, language = {en} } @misc{LehmannMartinMannigeletal., author = {Lehmann, Mario and Martin, Frank and Mannigel, Kerstin and Kaltschmidt, Kristin and Sack, Ulrich and Anderer, Ursula}, title = {Three-dimensional scaffold-free fusion culture: the way to enhanced chondrogenesis of in vitro propagated human articular chondrocytes}, series = {European Journal of Histochemistry}, volume = {57}, journal = {European Journal of Histochemistry}, number = {4}, issn = {2038-8306}, doi = {10.4081/ejh.2013.e31}, pages = {205 -- 215}, language = {en} } @misc{MartinLehmannSchlaegeretal., author = {Martin, Frank and Lehmann, Mario and Schl{\"a}ger, Peter and Sack, Ulrich and Anderer, Ursula}, title = {Differentiation capacity of chondrocytes in microtissues depends on TGF-ß subtyp}, series = {Journal of biochips \& tissue chips : JBTC}, volume = {S2}, journal = {Journal of biochips \& tissue chips : JBTC}, number = {002}, issn = {2153-0777}, doi = {10.4172/2153-0777.S2-002}, pages = {11}, language = {en} } @misc{KnuetterHiemannBrummaetal., author = {Kn{\"u}tter, Ilka and Hiemann, Rico and Brumma, Therese and B{\"u}ttner, Thomas and Großmann, Kai and Cusini, Marco and Pregnolato, Francesca and Borghi, Maria Orietta and Anderer, Ursula and Conrad, Karsten and Reinhold, Dirk and Roggenbuck, Dirk and Csernok, Elena}, title = {Automated interpretation of ANCA patterns - a new approach in the serology of ANCA-associated vasculitis}, series = {Arthritis Research \& Therapy}, volume = {14}, journal = {Arthritis Research \& Therapy}, issn = {1465-9913}, doi = {10.1186/ar4119}, pages = {10}, language = {en} } @misc{WillitzkiHiemannPetersetal., author = {Willitzki, Annika and Hiemann, Rico and Peters, Vanessa and Sack, Ulrich and Schierack, Peter and R{\"o}diger, Stefan and Anderer, Ursula and Conrad, Karsten and Bogdanos, Dimitrios Petrou and Reinhold, Dirk and Roggenbuck, Dirk}, title = {New PlatformTechnology for Comprehensive Serological Diagnostics of Autoimmune Diseases}, series = {Clinical and Developmental Immunology}, journal = {Clinical and Developmental Immunology}, issn = {1740-2530}, doi = {10.1155/2012/284740}, pages = {1 -- 8}, language = {en} } @misc{LutterScholkaRichteretal., author = {Lutter, Anne-Helen and Scholka, Jenny and Richter, Heiko and Anderer, Ursula}, title = {Applying XTT, WST-1, and WST-8 to human chondrocytes: A comparison of membrane-impermeable tetrazolium salts in 2D and 3D cultures}, series = {Clinical Hemorheology and Microcirculation}, volume = {67}, journal = {Clinical Hemorheology and Microcirculation}, number = {3-4}, issn = {1875-8622}, doi = {10.3233/CH-179213}, pages = {327 -- 342}, language = {en} } @misc{LutterHempelAndereretal., author = {Lutter, Anne-Helen and Hempel, Ute and Anderer, Ursula and Dieter, Peter}, title = {Biphasic influence of PGE2 on the resorption activity of osteoclast-like cells derived from human peripheral blood monocytes and mouse RAW264.7}, series = {Prostaglandins, Leukotrienes and Essential Fatty Acids}, volume = {111}, journal = {Prostaglandins, Leukotrienes and Essential Fatty Acids}, issn = {1532-2823}, doi = {10.1016/j.plefa.2016.03.017}, pages = {1 -- 7}, language = {en} } @misc{LutterHempelDieteretal., author = {Lutter, Anne-Helen and Hempel, Ute and Dieter, Peter and Anderer, Ursula}, title = {Multinuclearity is not mandatory for Bone Resorption Mononuclear Osteoclasts are able to resorbe Extracellular Bone Matrix in vitro}, series = {Bone-Tec, Munich, 2017}, journal = {Bone-Tec, Munich, 2017}, language = {en} } @misc{LutterScholkaRichteretal., author = {Lutter, Anne-Helen and Scholka, Jenny and Richter, Heiko and Anderer, Ursula}, title = {Comparison of membrane-impermeable tetrazolium salt assays in 2D and 3D cultures of human chondrocytes}, doi = {10.13140/RG.2.2.10682.49600}, language = {en} } @misc{LutterHempelDieteretal., author = {Lutter, Anne-Helen and Hempel, Ute and Dieter, Peter and Anderer, Ursula}, title = {The biomarker-based ODEM-assay affects cellular characteristics of osteoclasts in vitro Tissue culture polystyrene vs Osteoblast-Derived Extracellular Matrix}, doi = {10.13140/RG.2.2.20195.96806}, pages = {1}, language = {en} } @inproceedings{LutterHempelDieteretal., author = {Lutter, Anne-Helen and Hempel, Ute and Dieter, Peter and Anderer, Ursula}, title = {Cost-efficient diagnostic platform to measure osteoclast activity}, series = {35. Jahrestagung der Deutschen Gesellschaft f{\"u}r Klinische Mikrozirkulation und H{\"a}morheologie, Mainz, 2016}, booktitle = {35. Jahrestagung der Deutschen Gesellschaft f{\"u}r Klinische Mikrozirkulation und H{\"a}morheologie, Mainz, 2016}, doi = {10.13140/RG.2.2.10550.19528}, language = {en} } @inproceedings{LehmannVossMartinetal., author = {Lehmann, Mario and Voß, Fr{\"a}nzi and Martin, Frank and Schl{\"a}ger, Peter and Anderer, Ursula}, title = {Chondrogenic microtissues in the lab \& for the clinic}, series = {3D cell culture 2016, book of abstracts, DECHEMA Biotechnologie}, booktitle = {3D cell culture 2016, book of abstracts, DECHEMA Biotechnologie}, publisher = {DECHEMA e.V.}, address = {Frankfurt am Main}, isbn = {978-3-89746-182-6}, pages = {52 -- 53}, language = {en} } @inproceedings{LehmannMartinScholkaetal., author = {Lehmann, Mario and Martin, Frank and Scholka, Jenny and Petto, Carola and Wittenburg, Gretel and Sack, Ulrich and Anderer, Ursula}, title = {Human chondrocytes and dental pulp stem cells enhance their chondrogenic differentiation potential via a two-step fusion culture technique}, series = {Tissue Engineering, Part A, Vol. 21, Suppl. 1}, booktitle = {Tissue Engineering, Part A, Vol. 21, Suppl. 1}, doi = {10.1089/ten.tea.2015.5000.abstracts}, pages = {S-268 -- S-269}, language = {en} } @inproceedings{LehmannVossMartinetal., author = {Lehmann, Mario and Voß, Fr{\"a}nzi and Martin, Frank and Richter, Heiko and Anderer, Ursula}, title = {Chondrogenic microtissues with a lower differentiation degree used as in vitro transplants show improved integration in the tissue repair process in an in vitro therapy culture system}, series = {Regenerative Medicine}, volume = {10}, booktitle = {Regenerative Medicine}, number = {7S}, issn = {1746-0751}, pages = {S. 53}, language = {en} } @misc{LutterLiedtkeEckeetal., author = {Lutter, Anne-Helen and Liedtke, Victoria and Ecke, Annemarie and Scholka, Jenny and Muschter, Antje and Hentschel, Erik and Becker, Roland and Anderer, Ursula}, title = {Direct co-culture of human chondrogenic microtissues with osteoblast-like Saos-2 cells positively influence the differentiation potential of cartilage cells in 3D culture}, doi = {10.1007\%2Fs10354-018-0664-9.pdf}, pages = {S. 51}, language = {en} } @misc{LutterLiedtkeScholkaetal., author = {Lutter, Anne-Helen and Liedtke, Victoria and Scholka, Jenny and Muschter, Antje and Becker, Roland and Anderer, Ursula}, title = {Co-culture of human chondrogenic microtissues with osteoblast-like Saos-2 cells or HFF-1 fibroblasts influence the differentiation potential of spheroids}, series = {Journal of Cellular Biotechnology}, volume = {4}, journal = {Journal of Cellular Biotechnology}, number = {1/2}, issn = {2352-3697}, doi = {10.3233/JCB-189005}, pages = {31 -- 42}, abstract = {BACKGROUND: Tissue engineering has become a major field of research in biotechnology and biomedicine. As a consequence, cell-based therapeutic approaches are entering the hospitals, especially for skeletal regeneration. Traumatic injuries of cartilage are treated with autologous cell suspensions or in vitro generated cartilage tissues, but there is actually no therapy available for degenerative cartilage defects. However, Osteoarthritis (OA) is a major public health problem in the world affecting 240 million people globally. OBJECTIVE: To develop suitable in vitro tissues, the properties of chondrogenic spheroids should be optimized via coculture with cells naturally occurring as joint neighbours. METHODS: Human chondrocytes were isolated from condyles and propagated in monolayer culture. Scaffold-free spheroids were generated and co-cultured with joint-specific partner cells (osteoblast-like osteosarcoma cells, fibroblasts). Morphology and differentiation was analyzed using histochemistry (Alcian blue, Safranin O) and immunohistochemistry for cartilage markers (collagen type II, Sox9, proteoglycan), proliferation-associated protein (Ki67) and markers of connective tissue (collagen type I and actin). RESULTS: The provision of a more natural microenvironment in vitro via co-culture of chondrocyte-based aggregates with osteoblast-like Saos-2 cells enhanced the differentiation potential of chondrogenic spheroids towards hyaline cartilage. CONCLUSIONS: The study showed the positive influence of Saos-2 cells on the differentiation potential of human chondrocytes in co-culture.}, language = {en} }