@misc{MirskySokolovMarkinetal., author = {Mirsky, Vladimir M. and Sokolov, Valeri S. and Markin, Vladislav S. and Chekulaeva, L. N.}, title = {Bilayer lipid membrane formation from bacteriorhodopsin suspension in lipid solution}, series = {Biological membranes}, volume = {1}, journal = {Biological membranes}, issn = {0233-4755}, pages = {1143 -- 1150}, language = {en} } @misc{MirskySokolovMarkin, author = {Mirsky, Vladimir M. and Sokolov, Valeri S. and Markin, Vladislav S.}, title = {Reconstitution of bacteriorhodopsin in bilayer lipid membrane by monolayer method}, series = {Soviet electrochemistry}, volume = {20}, journal = {Soviet electrochemistry}, pages = {876}, language = {en} } @misc{MirskySokolovMelniketal., author = {Mirsky, Vladimir M. and Sokolov, Valeri S. and Melnik, E. I. and Diukova, T. V.}, title = {A study of incorporation of bacteriorhodopsin into bimolecular lipid membranes}, series = {Biophysics}, volume = {29}, journal = {Biophysics}, issn = {0006-3029}, pages = {250 -- 253}, language = {en} } @misc{MirskySokolov, author = {Mirsky, Vladimir M. and Sokolov, Valeri S.}, title = {Criteria of bacteriorhodopsin incorporation into bimolecular lipid membrane}, series = {Biophysics}, volume = {29}, journal = {Biophysics}, issn = {0006-3029}, pages = {246 -- 249}, language = {en} } @misc{MirskySokolovDiukovaetal., author = {Mirsky, Vladimir M. and Sokolov, Valeri S. and Diukova, T. V. and Melnik, E. I.}, title = {A study of bacteriorhodopsin-containing proteoliposome incorporation into bimolecular lipid membranes}, series = {Bioelectrochemistry and Bioenergetics}, volume = {11}, journal = {Bioelectrochemistry and Bioenergetics}, number = {4-6}, issn = {0302-4598}, pages = {327 -- 346}, abstract = {The photoresponses of planar bimolecular lipid membranes (BLM) modified with liposomes containing bacteriorhodopsin (BR) were investigated in the presence of Ca 2+ cations. Short-circuit currents during switch-on and switch-off of the light were measured within a time scale exceeding the BR photocycle, and the dependence of the currents on external field and membrane conductance was studied. A comparison of the experimental data with computed values indicates that the photoresponses observed are due not only to BR contained in the proteoliposomes adhered to the BLM, as generally believed, but also to BR in BLM itself. The transfer of BR to BLM may result from the complete fusion of liposomes with the planar membrane. The existence of such a transfer is indicative of the possibility of obtaining the most convenient model system for BR studies: a planar bimolecular lipid membrane containing incorporated BR.}, language = {en} } @misc{EfremenkoMirsky, author = {Efremenko, Yulia and Mirsky, Vladimir M.}, title = {Virtual sensor array consisting of a single sensor element with variable affinity: an application for analysis of fish freshness}, series = {Sensors and Actuators B: Chemical}, volume = {241}, journal = {Sensors and Actuators B: Chemical}, issn = {0925-4005}, doi = {10.1016/j.snb.2016.10.126}, pages = {652 -- 657}, abstract = {Recently reported concept of electrical control of sensor affinity was applied for formation of a virtual sensor array based on the single sensing element. Affinity properties of this element were modulated by electrically controlled conversion of chemosensitive material between its different redox states possessing different affinity. The sensor was realized on the basis of electrochemical chemotransistor in which a low temperature ionic liquid containing chloride was used to connect the chemosensitive material to the Ag/AgCl reference electrode. The concept of virtual array was proved by its application for monitoring of fish headspace. Using three characteristics of the sensor response measured at three different redox states of the same sensor material, we have obtained signals from a virtual sensor array consisting of nine chemosensitive elements. The sensor displays systematic changes of its nine signals during fish degradation and allows us to make quantitative analysis of its freshness defined as the after the catch. The level of detection is far below the level of organoleptic detection.}, language = {en} } @misc{ShishkanovaHavlikKraletal., author = {Shishkanova, Tatiana V. and Havlik, Martin and Kr{\´a}l, Vladim{\´i}r and Kopeck{\´y}, Dušan and Matějka, Pavel and Dendisov{\´a}, Marcela and Mirsky, Vladimir M.}, title = {Amino-substituted Tr{\"o}ger's base: electrochemical polymerization and characterization of the polymer film}, series = {Electrochimica Acta}, volume = {224}, journal = {Electrochimica Acta}, issn = {0013-4686}, doi = {10.1016/j.electacta.2016.12.061}, pages = {439 -- 445}, abstract = {The amino-substituted coumarin derivative of Tr{\"o}ger's base (CTB) was electrochemically polymerized on the surface of gold electrodes. The obtained polymer films were characterized by SEM, Raman spectroscopy, cyclic voltammetry and in-situ conductometry. Spectroscopic analysis indicates that the polymerization of CTB takes place via oxidation of the aniline fragment of CTB molecule, similar to the polymerization of aniline. The similarity of the polymer backbone to that of the polyaniline is also confirmed by the influence of potential on the film's conductivity, however the conductivity of the new material is in principle lower than that of polyaniline. SEM shows that the polymer forms a thin layer with a thickness of ~ 100 nm. Possible applications of the new material as an artificial receptor for aromatic analytes containing carboxy-group are proposed.}, language = {en} } @misc{NizamovScherbahnMirsky, author = {Nizamov, Shavkat and Scherbahn, Vitali and Mirsky, Vladimir M.}, title = {Ionic referencing in surface plasmon microscopy: visualization of the difference in surface properties of patterned monomolecular layers}, series = {Analytical Chemistry}, volume = {89}, journal = {Analytical Chemistry}, number = {7}, issn = {0003-2700}, doi = {10.1021/acs.analchem.7b00251}, pages = {3873 -- 3878}, abstract = {An approach for visualization of patterned monomolecular layers in surface plasmon microscopy (SPM) is suggested. The development of hidden image in SPM is achieved by referencing of images obtained in the presence of electrolytes with a high molar refraction of either anions or cations. A formation of diffuse layer near the charged surface areas leads to the redistribution of ions. The ratio of SPM images allows one to visualize this redistribution and to distinguish surface areas with different properties. The approach is unobtrusive and robust; it can be used with most SPR imaging instruments.}, language = {en} } @inproceedings{NizamovScherbahnMirsky, author = {Nizamov, Shavkat and Scherbahn, Vitali and Mirsky, Vladimir M.}, title = {Advanced wide-field surface plasmon microscopy of single adsorbing nanoparticles}, series = {Optical Sensors 2017, 24-27 April 2017 Prague, Czech Republic}, booktitle = {Optical Sensors 2017, 24-27 April 2017 Prague, Czech Republic}, publisher = {SPIE}, address = {Bellingham, Washington}, isbn = {978-1-5106-0963-1}, doi = {10.1117/12.2267144}, abstract = {In-situ detection and characterization of nanoparticles in biological media as well as in food or other complex samples is still a big challenge for existing analytical methods. Here we describe a label-free and cost-effective analytical method for detection of nanoparticles in the concentration range 106 -1010 NPs/ml. The proposed method is based on the surface plasmon resonance microscopy (SPRM) with a large field of view (~1.3mm2 ). It is able to detect and count adsorbing nanoparticles individually, totally up to the hundreds of thousands of NPs on the sensor surface. At constant diffusion conditions the detection rate is proportional to the number concentration of NPs, this provides an approach to determine the NPs concentration. The adsorption of nanoparticle can be manipulated by the surface functionalization, pH and electrolyte concentration of suspensions. Images of detected nanoparticles can be quantified in order to characterize them individually. The image intensity grows quasi-linearly with nanoparticle size for the given material. However, the size and material of nanoparticle cannot be resolved directly from the image. For determination of chemical composition, SPRM can be assisted by electrochemical analysis. In this case, the gold sensor surface is used both as a resonant media for plasmon microscopy and as a working electrode. Under potential sweep, the adsorbed NPs can be subjected to electrochemical dissolution, which is detected optically. The potential of this conversion characterizes the material of NPs.}, language = {en} } @inproceedings{NizamovScherbahnMirsky, author = {Nizamov, Shavkat and Scherbahn, Vitali and Mirsky, Vladimir M.}, title = {Wide-field surface plasmon microscopy of nano- and microparticles: features, benchmarking, limitations, and bioanalytical applications}, series = {Optical Sensors 2017, 24-27 April 2017, Prague, Czech Republic}, booktitle = {Optical Sensors 2017, 24-27 April 2017, Prague, Czech Republic}, publisher = {SPIE}, address = {Bellingham, Washington, USA}, isbn = {978-1-5106-0963-1}, doi = {10.1117/12.2267158}, abstract = {Detection of nano- and micro-particles is an important task for chemical analytics, food industry, biotechnology, environmental monitoring and many other fields of science and industry. For this purpose, a method based on the detection and analysis of minute signals in surface plasmon resonance images due to adsorption of single nanopartciles was developed. This new technology allows one a real-time detection of interaction of single nano- and micro-particles with sensor surface. Adsorption of each nanoparticle leads to characteristic diffraction image whose intensity depends on the size and chemical composition of the particle. The adsorption rate characterizes volume concentration of nano- and micro-particles. Large monitored surface area of sensor enables a high dynamic range of counting and to a correspondingly high dynamic range in concentration scale. Depending on the type of particles and experimental conditions, the detection limit for aqueous samples can be below 1000 particles per microliter. For application of method in complex media, nanoparticle images are discriminated from image perturbations due to matrix components. First, the characteristic SPRM images of nanoparticles (templates) are collected in aqueous suspensions or spiked real samples. Then, the detection of nanoparticles in complex media using template matching is performed. The detection of various NPs in consumer products like cosmetics, mineral water, juices, and wines was shown at sub-ppb level. The method can be applied for ultrasensitive detection and analysis of nano- and micro-particles of biological (bacteria, viruses, endosomes), biotechnological (liposomes, protein nanoparticles for drug delivery) or technical origin.}, language = {en} } @misc{LaurinavichyuteNizamovMirsky, author = {Laurinavichyute, Veronika K. and Nizamov, Shavkat and Mirsky, Vladimir M.}, title = {The Role of Anion Adsorption in the Effect of Electrode Potential on Surface Plasmon Resonance Response}, series = {ChemPhysChem : a European journal of physical chemistry and chemical physics}, volume = {18}, journal = {ChemPhysChem : a European journal of physical chemistry and chemical physics}, number = {12}, issn = {1439-7641}, doi = {10.1002/cphc.201601288}, pages = {1552 -- 1560}, abstract = {Surface plasmon resonance, being widely used in bioanalytics and biotechnology, is influenced by the electrical potential of the resonant gold layer. To evaluate the mechanism of this effect, we have studied it in solutions of various inorganic electrolytes. The magnitude of the effect decreases according to the series: KBr>KCl>KF>NaClO4. The data were treated by using different models of the interface. A quantitative description was obtained for the model, which takes into account the local dielectric function of gold being affected by the free electron charge, diffuse ionic layer near the gold/water interface, and specific adsorption of halides to the gold surface with partial charge transfer. Taking into account that most biological experiments are performed in chloride-containing solutions, detailed analysis of the model at these conditions was performed. The results indicate that the chloride adsorption is the main mechanism for the influence of potential on the surface plasmon resonance. The dependencies of surface concentration and residual charge of chloride on the applied potential were determined.}, language = {en} } @periodical{OPUS4-21635, title = {Microchimica Acta}, editor = {Mirsky, Vladimir M.}, publisher = {Springer}, address = {Heidelberg [u.a.]}, issn = {1436-5073}, abstract = {181.2014-184.2017}, language = {en} } @periodical{OPUS4-21639, title = {Russian Journal of Electrochemistry}, editor = {Mirsky, Vladimir M.}, publisher = {Springer}, address = {Heidelberg}, issn = {1023-1935}, abstract = {52.2016-53.2017}, language = {en} } @periodical{OPUS4-21641, title = {Sensors}, editor = {Mirsky, Vladimir M.}, publisher = {MDPI}, address = {Basel}, issn = {1424-8220}, abstract = {9.2009-17.2017}, language = {en} } @periodical{OPUS4-21666, title = {Journal of Biological Physics and Chemistry}, editor = {Mirsky, Vladimir M.}, publisher = {JBPC}, address = {Basel}, issn = {1512-0856}, abstract = {8.2008-17.2017}, language = {en} } @periodical{OPUS4-21669, title = {Journal of Functional Biomaterials}, editor = {Mirsky, Vladimir M.}, publisher = {MDPI}, address = {Basel}, issn = {2079-4983}, abstract = {5.2014-8.2017}, language = {en} } @misc{MirskyRoedigerSchieracketal., author = {Mirsky, Vladimir M. and R{\"o}diger, Stefan and Schierack, Peter and Roggenbuck, Dirk}, title = {Preface to the Special Issue on PCR on chip and related technologies}, series = {Microchimica Acta}, volume = {181}, journal = {Microchimica Acta}, number = {13/14}, doi = {10.1007/s00604-014-1246-1}, pages = {1609 -- 1610}, language = {en} } @incollection{ScherbahnNizamovMirsky, author = {Scherbahn, Vitali and Nizamov, Shavkat and Mirsky, Vladimir M.}, title = {Toward ultrasensitive surface plasmon resonance sensors}, series = {Label-free biosensing : advanced materials, devices and applications}, booktitle = {Label-free biosensing : advanced materials, devices and applications}, editor = {Sch{\"o}ning, Michael J. and Poghossian, Arshak}, publisher = {Springer}, address = {Cham}, isbn = {978-3-319-75220-4}, doi = {https://doi.org/10.1007/5346_2017_21}, pages = {409 -- 448}, abstract = {Despite the history of application of surface plasmon resonance (SPR) for chemo- and biosensing being over 30 years long, the development of this technique is still in progress. This review is focused on the technological aspects of further improvement of analytical performance of SPR transducers based on Kretschmann configuration. We describe basic measurement configurations, their improvements and optimizations, and their drawbacks and limitations. An importance of referencing in SPR sensors is highlighted. The referencing approaches are classified into the following domains: (1) macroscopic spatially separated referencing, (2) self-referencing based on micro-patterning, (3) in-place referencing, (4) spatiotemporal referencing, and (5) electrochemically assisted referencing. The underlying principles of these approaches, examples of their implementation, and resulting improvements of sensor performance are described. Finally, an analysis of SPR data and an extraction of affinity properties are discussed.}, language = {en} } @misc{GuticKozlicaBajukBogdanovicetal., author = {Gutic, Sanjin J. and Kozlica, Dzevad K. and Bajuk-Bogdanovic, Danica and Mitric, Miodrag and Mirsky, Vladimir M. and Mentus, Slavko V. and Pasti, Igor A.}, title = {Electrochemical tuning of capacitive response of graphene oxide}, series = {Physical Chemistry, Chemical Physics}, volume = {35}, journal = {Physical Chemistry, Chemical Physics}, number = {20}, issn = {1463-9084}, doi = {10.1039/C8CP03631D}, pages = {22698 -- 22709}, abstract = {The increasing energy demands of modern society require a deep understanding of the properties of energy storage materials, as well as the tuning of their performance. We show that the capacitance of graphene oxide (GO) can be precisely tuned using a simple electrochemical reduction route. In situ resistance measurements, in combination with cyclic voltammetry measurements and Raman spectroscopy, have shown that upon reduction GO is irreversibly deoxygenated, which is further accompanied by structural ordering and an increase in electrical conductivity. The capacitance is maximized when the concentration of oxygen functional groups is properly balanced with the conductivity. Any further reduction and deoxygenation leads to a gradual loss of capacitance. The observed trend is independent of the preparation route and the exact chemical and structural properties of GO. It is proposed that an improvement in the capacitive properties of any GO can be achieved by optimization of its reduction conditions.}, language = {en} } @misc{HammamiRaouafiMirsky, author = {Hammami, Asma and Raouafi, Noureddine and Mirsky, Vladimir M.}, title = {Electrically controlled Michael addition: Addressing of covalent immobilization of biological receptors}, series = {Biosensors and Bioelectronics}, volume = {121}, journal = {Biosensors and Bioelectronics}, issn = {1873-4235}, doi = {10.1016/j.bios.2018.08.044}, pages = {72 -- 79}, abstract = {Electrically addressed covalent immobilization of biomolecules to the defined electrodes of an electrode array is described. It is based on Michael addition of the thiol group of biomolecules to α,β-unsaturated carbonyl groups of benzoquinone. This "click" reaction was tested by immobilization of a number of thiolated compounds on the simplest array consisting of two gold electrodes coated by a self-assembled monolayer of benzoquinone-terminated hexanethiol. Electrically controlled binding of hexanethiol, ferrocenylhexanethiol, human serum albumin and thiol-terminated single-stranded DNA (ssDNA) was investigated. The binding was studied using cyclic voltammetry, X-ray photoelectron spectroscopy and surface plasmon resonance. The reaction requires the oxidized state of the benzoquinone moiety; this can be reached by applying of a moderate anodic potential to the electrode. Surface plasmon resonance measurements demonstrated that the thiol-modified ssDNA immobilized by this technique binds complementary synthetic oligonucleotides or PCR-amplified DNA fragments. The developed technology of electrical addressing of covalent immobilization can be applied for fabrication of sensor arrays.}, language = {en} }