@misc{KuhnJenschFischeretal., author = {Kuhn, Ramona and Jensch, Robert and Fischer, Thomas and Keuler, Klaus and Bryant, Isaac Mbir and Martienssen, Marion}, title = {Sunlight Degradation of the Aminophosphonate Diethylenetriamine Penta-(Methylenephosphonic Acid)}, series = {Solar}, volume = {2}, journal = {Solar}, number = {2}, issn = {2673-9941}, doi = {10.3390/solar2020009}, pages = {141 -- 157}, abstract = {Aminophosphonate diethylenetriamine penta(methylenephosphonic acid) (DTPMP) is a scale inhibitor commonly used in several industries. DTPMP is suspected to cause anthropogenic pollution through discharge into the aquatic environment. DTPMP is assumed to be degraded by sunlight radiation. We recently predicted a preliminary degradation pathway of DTPMP applyingUV treatment. Currently, we have not yet evidenced that DTPMP shows the same degradation pattern with natural sunlight. One major reason leads to the fact that the light spectrum emitted by UV lamps does not completely represent the natural sunlight spectrum, and the emitted UVB and UVA irradiation flux is much higher than for solar light. For that reason, the degradation pattern and kinetics might be different between artificial UV treatment and natural sunlight treatment. Here, we investigated whether DTPMP is degradable under natural sunlight radiation, and whether the degradation mechanisms determined through UV treatment are transferable to sunlight. We investigated five different treatment conditions, i.e., DTPMP degradation in direct or diffuse sunlight, in diffuse sunlight with addition of Ca2+ or Mg2+, and in diffuse sunlight with local TW. Our experiment was carried out from March 2021 to October 2021. We performed LC/MS analyses and measured the release of o-PO4 3 . DTPMP was degraded with all five treatment conditions. The fastest DTPMP degradation occurred in direct and diffuse sunlight without addition of bivalent cations. The addition of Ca2+ and Mg2+ resulted in inhibited degradation. Similar effects occurred for sunlight treatment with local TW. We evidenced different degradation mechanisms for DTPMP depending on the presence of alkaline earth metals as we previously proposed for UV-treated DTPMP. However, both degradation mechanisms of DTPMP belong to the same degradation pathway determined with UV treatment. Therefore, we conclude that DTPMP undergoes a similar degradation pathway in sunlight as compared to UV light.}, language = {en} } @misc{OkofoBeduAddoMartienssen, author = {Okofo, Louis Boansi and Bedu-Addo, Kenneth and Martienssen, Marion}, title = {Characterization of groundwater in the "Tamnean" Plutonic Suiteaquifers unsing hydrogeochemical and multivariate statistical evidence:a study in the Garu-Tempane District, Upper East Region of Ghana}, series = {Applied Water Science}, volume = {12}, journal = {Applied Water Science}, number = {2}, issn = {2190-5495}, doi = {10.1007/s13201-021-01559-2}, language = {en} } @misc{Rathsack, author = {Rathsack, Kristina}, title = {Unerw{\"u}nschte Bewohner : Wasserasseln und andere Makroorganismen in Trinkwasserversorgungsnetzen}, series = {IKZ-Fachplaner : Magazin f{\"u}r Planer, Berater und Entscheider der Geb{\"a}udetechnik}, volume = {7}, journal = {IKZ-Fachplaner : Magazin f{\"u}r Planer, Berater und Entscheider der Geb{\"a}udetechnik}, number = {11}, pages = {8 -- 12}, language = {de} } @inproceedings{BoellmannMartienssenNixdorf, author = {B{\"o}llmann, J{\"o}rg and Martienssen, Marion and Nixdorf, Brigitte}, title = {Stickstoffums{\"a}tze in unterschiedlichen Gew{\"a}ssertypen Berlin/Brandenburgs, Teil 1: Zellzahlen von Nitrifikanten und Denitrifikanten}, series = {Erweiterte Zusammenfassung der Jahrestagung der DGL 2011 in Weihenstephan}, booktitle = {Erweiterte Zusammenfassung der Jahrestagung der DGL 2011 in Weihenstephan}, publisher = {Eigenverlag der DGL}, address = {Hardegsen}, isbn = {978-3-9813095-2-2}, pages = {234 -- 238}, language = {de} } @misc{MartienssenSchulze, author = {Martienssen, Marion and Schulze, Rolf}, title = {Langzeituntersuchungen zum Einsatz von Rekultivierungs-/Methanoxidationsschichten auf Hausm{\"u}lldeponien}, series = {M{\"u}ll und Abfall}, volume = {44}, journal = {M{\"u}ll und Abfall}, number = {4}, issn = {1863-9763}, pages = {202 -- 209}, language = {de} } @phdthesis{Ebert, author = {Ebert, Sebastian}, title = {Potentiale und Grenzen eines neuen Verfahrens zum linienhaften Gasmonitoring in B{\"o}den}, address = {Cottbus}, pages = {114}, language = {de} } @misc{SarpongAmankwaaMartienssenetal., author = {Sarpong, Daniella and Amankwaa, Gordon and Martienssen, Marion and Burkhardt, Marko}, title = {A novel biomethane (BMP) and somposting (CMP) potential framework for determining biogas and composting potential of urban organic waste}, series = {Environmental Technology}, volume = {45(2024)}, journal = {Environmental Technology}, number = {8}, issn = {1479-487X}, doi = {10.1080/09593330.2022.2145241}, pages = {1471 -- 1482}, language = {en} } @misc{RiedelCommichauBenndorfetal., author = {Riedel, Ramona and Commichau, Fabian M. and Benndorf, Dirk and Hertel, Robert and Holzer, Katharina and Mardoukhi, Mohammad Saba Yousef and Noack, Laura and Martienssen, Marion}, title = {Biodegradation of selected aminophosphonates by the bacterial isolate Ochrobactrum sp. BTU1}, series = {Microbial Research}, volume = {280}, journal = {Microbial Research}, issn = {0944-5013}, doi = {10.1016/j.micres.2024.127600}, pages = {1 -- 12}, abstract = {Aminophosphonates, like glyphosate (GS) or metal chelators such as ethylenediaminetetra(methylenephosphonic acid) (EDTMP), are released on a large scale worldwide. Here, we have characterized a bacterial strain capable of degrading synthetic aminophosphonates. The strain was isolated from LC/MS standard solution. Genome sequencing indicated that the strain belongs to the genus Ochrobactrum. Whole-genome classification using pyANI software to compute a pairwise ANI and other metrics between Brucella assemblies and Ochrobactrum contigs revealed that the bacterial strain is designated as Ochrobactrum sp. BTU1. Degradation batch tests with Ochrobactrum sp. BTU1 and the selected aminophosphonates GS, EDTMP, aminomethylphosphonic acid (AMPA), iminodi(methylene-phosphonic) (IDMP) and ethylaminobis(methylenephosphonic) acid (EABMP) showed that the strain can use all phosphonates as sole phosphorus source during phosphorus starvation. The highest growth rate was achieved with AMPA, while EDTMP and GS were least supportive for growth. Proteome analysis revealed that GS degradation is promoted by C-P lyase via the sarcosine pathway, i.e., initial cleavage at the C-P bond. We also identified C-P lyase to be responsible for degradation of EDTMP, EABMP, IDMP and AMPA. However, the identification of the metabolite ethylenediaminetri(methylenephosphonic acid) via LC/MS analysis in the test medium during EDTMP degradation indicates a different initial cleavage step as compared to GS. For EDTMP, it is evident that the initial cleavage occurs at the C-N bond. The detection of different key enzymes at regulated levels, form the bacterial proteoms during EDTMP exposure, further supports this finding.}, language = {en} } @misc{HertelSchoeneMittelstaedtetal., author = {Hertel, Robert and Sch{\"o}ne, Kerstin and Mittelst{\"a}dt, Carolin and Meißner, Janek and Zschoche, Nick and Collignon, Madeline and Kohler, Christian and Friedrich, Ines and Schneider, Dominik and Hoppert, Michael and Kuhn, Ramona and Schwedt, Inge and Scholz, Patricia and Poehlein, Anja and Martienssen, Marion and Ischebeck, Till and Daniel, Rolf and Commichau, Fabian M.}, title = {Characterization of glyphosate-resistant Burkholderia anthina and Burkholderia cenocepacia isolates from a commercial Roundup® solution}, series = {Environmental Microbiology Reports}, volume = {14}, journal = {Environmental Microbiology Reports}, number = {1}, issn = {1758-2229}, doi = {10.1111/1758-2229.13022}, pages = {70 -- 84}, abstract = {Roundup® is the brand name for herbicide solutions containing glyphosate, which specifically inhibits the 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase of the shikimate pathway. The inhibition of the EPSP synthase causes plant death because EPSP is required for biosynthesis of aromatic amino acids. Glyphosate also inhibits the growth of archaea, bacteria, Apicomplexa, algae and fungi possessing an EPSP synthase. Here, we have characterized two glyphosate-resistant bacteria from a Roundup solution. Taxonomic classification revealed that the isolates 1CH1 and 2CH1 are Burkholderia anthina and Burkholderia cenocepacia strains respectively. Both isolates cannot utilize glyphosate as a source of phosphorus and synthesize glyphosate-sensitive EPSP synthase variants. Burkholderia. anthina 1CH1 and B. cenocepacia 2CH1 tolerate high levels of glyphosate because the herbicide is not taken up by the bacteria. Previously, it has been observed that the exposure of soil bacteria to herbicides like glyphosate promotes the development of antibiotic resistances. Antibiotic sensitivity testing revealed that the only the B. cenocepacia 2CH1 isolate showed increased resistance to a variety of antibiotics. Thus, the adaptation of B. anthina 1CH1 and B. cenocepacia 2CH1 to glyphosate did not generally increase the antibiotic resistance of both bacteria. However, our study confirms the genomic adaptability of bacteria belonging to the genus Burkholderia.}, language = {en} } @misc{HertelGibhardtMartienssenetal., author = {Hertel, Robert and Gibhardt, Johannes and Martienssen, Marion and Kuhn, Ramona and Commichau, Fabian M.}, title = {Molecular mechanisms underlying glyphosate resistance in bacteria}, series = {Environmental Microbiology}, volume = {23}, journal = {Environmental Microbiology}, number = {6}, issn = {1462-2920}, doi = {10.1111/1462-2920.15534}, pages = {2891 -- 2905}, abstract = {Glyphosate is a nonselective herbicide that kills weeds and other plants competing with crops. Glyphosate specifically inhibits the 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase, thereby depleting the cell of EPSP serving as a precursor for biosynthesis of aromatic amino acids. Glyphosate is considered to be toxicologically safe for animals and humans. Therefore, it became the most-important herbicide in agriculture. However, its intensive application in agriculture is a serious environmental issue because it may negatively affect the biodiversity. A few years after the discovery of the mode of action of glyphosate, it has been observed that bacteria evolve glyphosate resistance by acquiring mutations in the EPSP synthase gene, rendering the encoded enzyme less sensitive to the herbicide. The identification of glyphosate-resistant EPSP synthase variants paved the way for engineering crops tolerating increased amounts of the herbicide. This review intends to summarize the molecular mechanisms underlying glyphosate resistance in bacteria. Bacteria can evolve glyphosate resistance by (i) reducing glyphosate sensitivity or elevating production of the EPSP synthase, by (ii) degrading or (iii) detoxifying glyphosate and by (iv) decreasing the uptake or increasing the export of the herbicide. The variety of glyphosate resistance mechanisms illustrates the adaptability of bacteria to anthropogenic substances due to genomic alterations.}, language = {en} }