@inproceedings{PohlertMartienssenWeissetal., author = {Pohlert, M. and Martienssen, Marion and Weiß, H. and Trabitzsch, R. and Geistlinger, Helmut}, title = {SF6-Gastracerversuche zur Absch{\"a}tzung der Raumwirkung bei Direktgasinjektionen zur Grundwassersanierung}, series = {Behandlungstechnologien f{\"u}r bergbaubeeinflusste W{\"a}sser, Proceedingsband zu den Workshops am Geologischen Institut der TU Bergakademie Freiberg am 12. und 13. Juni 2008}, booktitle = {Behandlungstechnologien f{\"u}r bergbaubeeinflusste W{\"a}sser, Proceedingsband zu den Workshops am Geologischen Institut der TU Bergakademie Freiberg am 12. und 13. Juni 2008}, publisher = {Institut f{\"u}r Geologie}, address = {Freiberg}, pages = {1141 -- 1148}, language = {de} } @misc{KochStraubMangoldetal., author = {Koch, Roland and Straub, Andrea and Mangold, Sylke and Sch{\"o}pke, Ralph and Martienssen, Marion}, title = {Tausalz, Temperatur und chemische P-F{\"a}llung}, series = {Wasserwirtschaft, Wassertechnik : wwt}, journal = {Wasserwirtschaft, Wassertechnik : wwt}, number = {11/12}, issn = {1438-5716}, pages = {41 -- 46}, language = {de} } @misc{LeschikMusolffMartienssenetal., author = {Leschik, Sebastian and Musolff, Andreas and Martienssen, Marion and Krieg, Ronald and Bayer-Raich, Marti and Reinstorf, Frido and Strauch, Gerhard and Schirmer, Mario}, title = {Investigation of sewer exfiltration using integral pumping tests and wastewater indicators}, doi = {10.1016/j.jconhyd.2009.10.001}, language = {de} } @misc{LeschikMusolffKriegetal., author = {Leschik, Sebastian and Musolff, Andreas and Krieg, Ronald and Martienssen, Marion and Bayer-Raich, Marti and Strauch, Gerhard and Schirmer, Mario and Reinstorf, Frido}, title = {Application of integral pumping tests to investigate the influence of a losing stream on groundwater quality}, doi = {10.5194/hess-13-1765-2009}, language = {de} } @misc{SchmidtKalbusMartienssenetal., author = {Schmidt, C. and Kalbus, Edda and Martienssen, Marion and Schirmer, M.}, title = {The influence of heterogeneous groundwater discharge an the timescales of contaminat mass flux from streambed sediments field evidence and long-term predictions}, series = {Hydrology and Earth Systems Sciences Discussion}, volume = {5}, journal = {Hydrology and Earth Systems Sciences Discussion}, number = {2}, pages = {971 -- 1001}, language = {en} } @misc{MartienssenKuklaBalckeetal., author = {Martienssen, Marion and Kukla, S. and Balcke, G. U. and Hasselwander, E. and Schirmer, M.}, title = {Evidence of natural occurring MTBE biodegradation by analysing metabolites and biodegradation products}, language = {en} } @misc{BeckmannGerhardtZittwitzetal., author = {Beckmann, Annika and Gerhardt, Matthias and Zittwitz, Mark and Martienssen, Marion and Krieg, Ronald and Geistlinger, Helmut and Schirmer, Mario}, title = {Das Oxywall-Projekt: Anwendung eines Verfahrens zur Direktgasinjektion von Sauerstoff zur in situ Sanierung von organisch kontaminierten Grundw{\"a}ssern}, series = {Altlastenspektrum}, volume = {16}, journal = {Altlastenspektrum}, number = {8}, issn = {0942-3818}, pages = {153 -- 159}, language = {de} } @misc{RingpfeilGerhardtZittwitzetal., author = {Ringpfeil, Manfred and Gerhardt, Matthias and Zittwitz, Mark and Beckmann, Annika and Geistlinger, Helmut and Schirmer, Mario and Martienssen, Marion}, title = {Verfahren und Anordnung zur Direktgasinjektion f{\"u}r die Sanierung von kontaminierten B{\"o}rden und/oder Grundwasserleitern sowie ein entsprechendes Computerprogramm und ein entsprechendes computerlesbares Speichermedium}, language = {de} } @inproceedings{Martienssen, author = {Martienssen, Marion}, title = {Erste Ergebnisse aus dem F+E-Thema "Methanoxidation in Rekultivierungs-/Wasserhaushaltsschichten in Sachsen-Anhalt}, series = {3. Leipziger Deponiefachtagung, 22/23.02.2007, Leipzig, Conference proceedings}, booktitle = {3. Leipziger Deponiefachtagung, 22/23.02.2007, Leipzig, Conference proceedings}, publisher = {HTWK}, address = {Leipzig}, language = {de} } @misc{BoeckelmannJankeKuhnetal., author = {B{\"o}ckelmann, Uta and Janke, Andrea and Kuhn, Ramona and Neu, Thomas R. and Wecke, J{\"o}rg and Lawrence, John R. and Szewzyk, Ulrich}, title = {Bacterial extracellular DNA forming a defined network-like structure}, language = {en} } @misc{GuiotKuhnLevesqueetal., author = {Guiot, Serge R. and Kuhn, Ramona and L{\´e}vesque, M. J. and Cimpoia, Ruxandra}, title = {Ultrastructure of a bioelectrolytic methanogenic/methanotrophic granular biofilm for the complete degradation of tetrachloroethylene in contaminated groundwater}, language = {en} } @misc{GuiotCimpoiaKuhnetal., author = {Guiot, Serge R. and Cimpoia, Ruxandra and Kuhn, Ramona and Alaplantive, Aude}, title = {Electrolytic Methanogenic-methanotropic coupling for Tetrachloroethylene Bioremediation: A Proof of Concept}, series = {Environmental science \& technology}, volume = {42}, journal = {Environmental science \& technology}, number = {8}, issn = {0013-936X}, pages = {3011 -- 3017}, language = {en} } @inproceedings{NixdorfBeckerGruenebergetal., author = {Nixdorf, Brigitte and Becker, Katja and Gr{\"u}neberg, Bj{\"o}rn and B{\"o}llmann, J{\"o}rg and Martienssen, Marion}, title = {Stickstoffums{\"a}tze in unterschiedlichen Gew{\"a}ssertypen Berlin/Brandenburgs, Teil 2: Erste Ergebnisse zu eintr{\"a}gen und Ums{\"a}tzen}, series = {Erweiterte Zusammenfassungen der Jahrestagung 2011 der Deutschen Gesellschaft f{\"u}r Limnologie (DGL) und der deutschen und {\"o}sterreichischen Sektion der Societas Internationalis Limnologiae (SIL), Weihenstephan, 12. - 16. Sept. 2011}, booktitle = {Erweiterte Zusammenfassungen der Jahrestagung 2011 der Deutschen Gesellschaft f{\"u}r Limnologie (DGL) und der deutschen und {\"o}sterreichischen Sektion der Societas Internationalis Limnologiae (SIL), Weihenstephan, 12. - 16. Sept. 2011}, publisher = {Eigenverl. der DGL}, address = {Hardegsen}, isbn = {978-3-9813095-2-2}, pages = {239 -- 244}, language = {de} } @inproceedings{BoellmannMartienssenGrueneberg, author = {B{\"o}llmann, J{\"o}rg and Martienssen, Marion and Gr{\"u}neberg, Bj{\"o}rn}, title = {Vergleich der mikrobiellen Nitratreduktion in Sediment und Pelagial eines eutrophen Flusssees und eines mesotrophen geschichteten Sees}, series = {Erweiterte Zusammenfassungen der Jahrestagungder Deutschen Gesellschaft f{\"u}r Limnologie (DGL), Potsdam - Berlin, 09. - 13. September 2013}, booktitle = {Erweiterte Zusammenfassungen der Jahrestagungder Deutschen Gesellschaft f{\"u}r Limnologie (DGL), Potsdam - Berlin, 09. - 13. September 2013}, publisher = {Hardegsen}, address = {Eigenverlag der DGL}, isbn = {978-3-9813095-5-3}, pages = {35 -- 39}, language = {de} } @misc{GoeringMartienssen, author = {G{\"o}ring, Daniel and Martienssen, Marion}, title = {Elimination von Epichlorhydrin bei der Behandlung industrieller Abw{\"a}sser}, series = {KA - Korrespondenz Abwasser, Abfall}, volume = {61}, journal = {KA - Korrespondenz Abwasser, Abfall}, number = {5}, issn = {1866-0029}, pages = {447 -- 455}, language = {de} } @inproceedings{BoellmannScholler, author = {B{\"o}llmann, J{\"o}rg and Scholler, Markus}, title = {Preliminary studies on the spread of the rust fungus Puccinia glechomatis in North America}, series = {Joint Meeting of the American Phytopathological Society, the Mycological Society of America, and the Society of Nematologists, Salt Lake City, Utah, USA, August 25-29, 2001}, booktitle = {Joint Meeting of the American Phytopathological Society, the Mycological Society of America, and the Society of Nematologists, Salt Lake City, Utah, USA, August 25-29, 2001}, pages = {S. 104}, language = {en} } @inproceedings{BoellmannScholler, author = {B{\"o}llmann, J{\"o}rg and Scholler, Markus}, title = {The Life Cycle of Puccinia glechomatis, a microcyclic rust on Glechoma hederacea}, series = {2002 MSA Annual Meeting Corvallis, OREGON}, booktitle = {2002 MSA Annual Meeting Corvallis, OREGON}, pages = {S. 21}, language = {en} } @misc{RathsackBoellmannMartienssen, author = {Rathsack, Kristina and B{\"o}llmann, J{\"o}rg and Martienssen, Marion}, title = {Comparative Study of Different Methods for Analyzing Denitrifying Bacteria in Fresh Water Ecosystems}, series = {Journal of Water Resource and Protection}, volume = {6}, journal = {Journal of Water Resource and Protection}, number = {6}, issn = {1945-3094}, pages = {609 -- 617}, abstract = {Bacteria capable of denitrification play a significant role in the nitrogen cycle of freshwater ecosystems. By metabolizing nitrogen compounds they e.g. counteract the eutrophication of natural waters. To get detailed insights into the in situ turnover rates of nitrogen a reliable tool of quantification for active microorganisms is essential. In the present investigation, quantification capabilities of a molecular tool (Polymerase Chain Reaction—PCR) and a cultivation based tool (Most probable number—MPN) were investigated and compared. The total bacterial concentration yielded by the molecular PCR approach was up to 6-fold higher compared to the results of the MPN approach. However, the portion of culturable denitrifying bacteria compared to the number of specific gene copies (nirS) was much lower. Depending on the environmental conditions, the difference between the PCR and the MPN approach was up to three orders of magnitude. From lab scale experiments with a pure P. aeroginosa strain it can be concludes, that these differences are not the result of inappropriate culture conditions but rather reflect the portion of so called viable but not culturable bacteria (VBNC). Low nitrate concentrations as found in many fresh water ecosystems induced a significant increase in the portion of non culturable denitrifying bacteria. Referred to the investigation of dynamic populations, the number of metabolic active bacteria is represented by the MPN rather than by the PCR approach.}, language = {en} } @misc{Rathsack, author = {Rathsack, Kristina}, title = {Unerw{\"u}nschte Bewohner : Wasserasseln und andere Makroorganismen in Trinkwasserversorgungsnetzen}, series = {IKZ Haustechnik : Magazin f{\"u}r Geb{\"a}ude- und Energietechnik, Sonderheft Trinkwasserhygiene 2013}, journal = {IKZ Haustechnik : Magazin f{\"u}r Geb{\"a}ude- und Energietechnik, Sonderheft Trinkwasserhygiene 2013}, number = {Sonderheft}, language = {de} } @misc{BoellmannSchoeller, author = {B{\"o}llmann, J{\"o}rg and Sch{\"o}ller, Markus}, title = {Life cycle and life strategy features of Puccinia glechomatis (Uredinales) favorable for extending the natural range of distribution}, series = {Mycoscience}, volume = {47}, journal = {Mycoscience}, number = {3}, issn = {1340-3540}, doi = {10.1007/s10267-006-0282-z}, pages = {152 -- 158}, language = {en} } @misc{RathsackRathsack, author = {Rathsack, Kristina and Rathsack, Udo}, title = {Besiedlung von Wasserversorgungssystemen durch Asseln und andere Invertebraten : Weiterentwickeltes Modell und Messsystem biologischer Prozesse}, series = {GWF : Wasser, Abwasser}, volume = {151}, journal = {GWF : Wasser, Abwasser}, number = {2}, issn = {0016-3651}, pages = {200 -- 207}, language = {de} } @misc{RathsackRathsack, author = {Rathsack, Kristina and Rathsack, Udo}, title = {Besiedlung von Wasserversorgungssystemen durch Asseln und andere Invertebraten}, series = {GWF : Wasser, Abwasser}, volume = {150}, journal = {GWF : Wasser, Abwasser}, number = {6}, issn = {0016-3651}, pages = {451 -- 458}, language = {de} } @misc{RathsackStackebrandtReitneretal., author = {Rathsack, Kristina and Stackebrandt, E. and Reitner, Joachim and Schumann, G.}, title = {Microorganisms Isolated from Deep Sea Low-temperature Influenced Oceanic Crust Basalts and Sediment Samples Collected along the Mid-Atlantic Ridge}, series = {Geomicrobiology journal}, volume = {26}, journal = {Geomicrobiology journal}, number = {4}, issn = {0149-0451}, pages = {264 -- 274}, language = {en} } @misc{NayerniaNolteMichelmannetal., author = {Nayernia, Karim and Nolte, Jessica and Michelmann, Hans W. and Lee, Jae Ho and Rathsack, Kristina and Drusenheimer, Nadja and Dev, Arvind and Wulf, Gerald and Ehrmann, Ingrid E. and Elliott, David J. and Okpanyi, Vera and Zechner, Ulrich and Haaf, Thomas and Meinhardt, Andreas and Engel, Wolfgang}, title = {In Vitro-Differentiated Embryonic Stem Cells Give Rise to Male Gametes that Can Generate Offspring Mice}, series = {Developmental Cell}, volume = {11}, journal = {Developmental Cell}, number = {1}, issn = {1534-5807}, pages = {125 -- 132}, language = {en} } @phdthesis{Rathsack, author = {Rathsack, Kristina}, title = {Microbial diversity within the low-temperature influenced deep marine biosphere along the Mid-Atlantic-Ridge}, pages = {85}, language = {en} } @misc{NolteRathsackDrusenheimeretal., author = {Nolte, Jessica and Rathsack, Kristina and Drusenheimer, Nadja and Dev, Arvind and Zechner, Ulrich and Haaf, Thomas and Meinhardt, Andreas and Michelmann, Hans W. and Nayernia, Karim and Engel, Wolfgang}, title = {M{\"a}nnliche Keimzellen aus embryonalen Stammzellen}, series = {Medizinische Genetik}, volume = {19}, journal = {Medizinische Genetik}, number = {1}, issn = {0936-5931}, pages = {10 -- 17}, language = {de} } @inproceedings{Boellmann, author = {B{\"o}llmann, J{\"o}rg}, title = {Die Bedeutung von Anthropochorie und die Verbreitung von infiziertem Wirtsgewebe am Beispiel des Rostpilzes Puccinia glechomatis}, series = {Treffpunkt biologische Vielfalt, aktuelle Forschung im Rahmen des {\"U}bereinkommens {\"u}ber die biologische Vielfalt vorgestellt auf einer wissenschaftlichen Expertentagung an der Internationalen Naturschutzakademie Insel Vilm vom 21. - 25. August 2006}, booktitle = {Treffpunkt biologische Vielfalt, aktuelle Forschung im Rahmen des {\"U}bereinkommens {\"u}ber die biologische Vielfalt vorgestellt auf einer wissenschaftlichen Expertentagung an der Internationalen Naturschutzakademie Insel Vilm vom 21. - 25. August 2006}, publisher = {Bundesamt f{\"u}r Naturschutz}, address = {Bonn [u.a.]}, pages = {107 -- 112}, language = {de} } @inproceedings{SchollerBoellmann, author = {Scholler, Markus and B{\"o}llmann, J{\"o}rg}, title = {Puccinia glechomatis and P. lagenophorae in North America: invasion patterns, life cycle and life strategies}, series = {Mycological Society of America (MSA) annual meeting, Hilo (Hawaii), 2006}, booktitle = {Mycological Society of America (MSA) annual meeting, Hilo (Hawaii), 2006}, pages = {S. 52}, language = {en} } @inproceedings{GeistlingerBeckmannGerhardtetal., author = {Geistlinger, Helmut and Beckmann, Annika and Gerhardt, Matthias and Martienssen, Marion and Schirmer, Mario}, title = {Das Feldexperiment OXYWALL: Direktgasinjektion von Sauerstoff zur in situ Sanierung von organisch kontaminierten Grundw{\"a}ssern}, series = {Behandlungstechnologien f{\"u}r bergbaubeeinflusste W{\"a}sser, GIS - Geowissenschaftliche Anwendungen und Entwicklungen, Proceedingsband zu den Workshops am Geologischen Institut der TU Bergakademie Freiberg am 14. und 15. Juni 2007}, booktitle = {Behandlungstechnologien f{\"u}r bergbaubeeinflusste W{\"a}sser, GIS - Geowissenschaftliche Anwendungen und Entwicklungen, Proceedingsband zu den Workshops am Geologischen Institut der TU Bergakademie Freiberg am 14. und 15. Juni 2007}, publisher = {Inst. f{\"u}r Geologie}, address = {Freiberg}, pages = {99 -- 106}, language = {de} } @phdthesis{Fichtner, author = {Fichtner, Nicole}, title = {Untersuchung zur Leistungsf{\"a}higkeit von Biofilmverfahren mit frei beweglichen Aufwuchsk{\"o}rpern bei der kommunalen Abwasserbehandlung}, address = {Cottbus}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:co1-opus4-34765}, language = {de} } @inproceedings{GeistlingerLazikEisemannetal., author = {Geistlinger, Helmut and Lazik, D. and Eisemann, D. and Beckmann, Annika and Martienssen, Marion and Schirmer, Mario and Zittwitz, Mark and Gerhardt, Matthias}, title = {Reaktive Gasw{\"a}nde mittels Direktgasinjektion: Vom Bench-Scale zum Field-Scale (OXYWALL)}, series = {Behandlungstechnologien f{\"u}r bergbaubeeinflusste W{\"a}sser, GIS - geowissenschaftliche Anwendungen und Entwicklungen, Proceedingsband zu den Workshops am Geologischen Institut der TU Bergakademie Freiberg am 22. und 23. Juni 2006}, booktitle = {Behandlungstechnologien f{\"u}r bergbaubeeinflusste W{\"a}sser, GIS - geowissenschaftliche Anwendungen und Entwicklungen, Proceedingsband zu den Workshops am Geologischen Institut der TU Bergakademie Freiberg am 22. und 23. Juni 2006}, publisher = {Inst. f{\"u}r Geologie}, address = {Freiberg}, pages = {159 -- 163}, language = {de} } @misc{RingpfeilGerhardtZittwitzetal., author = {Ringpfeil, Manfred and Gerhardt, Matthias and Zittwitz, Mark and Beckmann, Annika and Geistlinger, Helmut and Schirmer, Mario and Martienssen, Marion}, title = {Verfahren und Anordnung zur Direktgasinjektion f{\"u}r die Sanierung von kontaminierten B{\"o}den und /oder Grundwasserleitern sowie ein entsprechendes Computerprogramm und ein entsprechendes computerlesbares Speichermedium}, language = {de} } @inproceedings{MartienssenKoenig, author = {Martienssen, Marion and K{\"o}nig, W.}, title = {Online-Bestimmung von Nitrifikationsinhibitoren in biologischen Kl{\"a}ranlagen}, series = {Technische Systeme f{\"u}r Biotechnologie und Umwelt, 11. Heiligenst{\"a}dter Kolloquium, Heilbad Heiligenstadt, 30.09. - 02.10.2002}, booktitle = {Technische Systeme f{\"u}r Biotechnologie und Umwelt, 11. Heiligenst{\"a}dter Kolloquium, Heilbad Heiligenstadt, 30.09. - 02.10.2002}, publisher = {Institut f{\"u}r Bioprozess- und Analysenmesstechnik}, address = {Heiligenstadt}, isbn = {3-00-011287-1}, pages = {205 -- 210}, language = {de} } @misc{MartienssenKaiserSchirmeretal., author = {Martienssen, Marion and Kaiser, A. and Schirmer, M. and Graf, W. and Kutschke, S. and Schulze, R. and Thiede, K.}, title = {Untersuchungen zum Einsatz von Rekultivierungs-/Methanoxidationsschichten auf Deponien des Landes Sachsen-Anhalt}, series = {M{\"u}ll und Abfall}, volume = {40}, journal = {M{\"u}ll und Abfall}, number = {5}, pages = {247 -- 253}, language = {de} } @inproceedings{MartienssenKuklaBalckeetal., author = {Martienssen, Marion and Kukla, S. and Balcke, G. U. and Rohwerder, Thore and Haase, K. and Schirmer, M.}, title = {Enhanced Natural Attenuation of MTBE: Comparison of different technologies in field experiments at the Leuna site (Germany)}, series = {Second European Conference on MTBE, Barcelona, Spain, November 4 - 5, 2004}, booktitle = {Second European Conference on MTBE, Barcelona, Spain, November 4 - 5, 2004}, pages = {60 -- 64}, language = {en} } @inproceedings{RohwerderCeniniHeldetal., author = {Rohwerder, Thore and Cenini, V. and Held, C. and Martienssen, Marion and Lechner, U. and M{\"u}ller, R. H.}, title = {Novel MTBE-degrading bacterial isolate from Leuna groundwater (Germany): characterization of the degradation pathway with focus on HIBA oxidation}, series = {Second European Conference on MTBE, Barcelona, Spain, November 4 - 5, 2004}, booktitle = {Second European Conference on MTBE, Barcelona, Spain, November 4 - 5, 2004}, pages = {47 -- 49}, language = {en} } @inproceedings{MartienssenSchirmer, author = {Martienssen, Marion and Schirmer, M.}, title = {Comparison of different technologies to enhance the natural Attenuation of MTBE}, series = {9th International In Situ and On-Site Bioremediation Symposium, May 7 - 10, 2007, Baltimore, Maryland, USA}, booktitle = {9th International In Situ and On-Site Bioremediation Symposium, May 7 - 10, 2007, Baltimore, Maryland, USA}, publisher = {Curran}, address = {Redhook, NY}, isbn = {978-1-57477-161-9}, language = {en} } @misc{SchmidtKalbusKriegetal., author = {Schmidt, Christian and Kalbus, Edda and Krieg, Ronald and Bayer-Raich, Marti and Leschik, Sebastian and Reinstorf, Frido and Martienssen, Marion and Schirmer, Mario}, title = {Contaminant mass flow rates between groundwater, strembed sediments and surface water at the regionally contaminated site Bitterfeld}, series = {Grundwasser}, volume = {13}, journal = {Grundwasser}, number = {3}, issn = {1430-483X}, pages = {133 -- 146}, language = {en} } @misc{SchmidtKalbusMartienssenetal., author = {Schmidt, C. and Kalbus, Edda and Martienssen, Marion and Schirmer, Mario}, title = {The influence of heterogeneous groundwater discharge on the timescales of contaminant mass flux from streambed sediments field evidence and long-term predictions}, series = {Hydrology and Earth System Sciences Discussions}, volume = {5}, journal = {Hydrology and Earth System Sciences Discussions}, number = {2}, issn = {1812-2116}, pages = {971 -- 1001}, language = {en} } @misc{MuhondwaMartienssenBurkhardt, author = {Muhondwa, Jacob Paul and Martienssen, Marion and Burkhardt, Marko}, title = {Feasibility of Anaerobic Digestion of Potato peels for Biogas as Mitigation of Greenhouse gases Emission Potential}, series = {International journal of environmental research}, volume = {9}, journal = {International journal of environmental research}, number = {2}, issn = {1735-6865}, pages = {481 -- 488}, language = {en} } @misc{MuhondwaMartienssenBurkhardt, author = {Muhondwa, Jacob Paul and Martienssen, Marion and Burkhardt, Marko}, title = {Effect of high biowaste moisturie content on phatogens hygienization potential of in-vessel composting technology during compost production}, series = {International Journal of Current Research}, volume = {7}, journal = {International Journal of Current Research}, number = {4}, issn = {0975-833X}, pages = {15042 -- 15048}, language = {en} } @misc{BoellmannRathsackMartienssen, author = {B{\"o}llmann, J{\"o}rg and Rathsack, Kristina and Martienssen, Marion}, title = {The precision of bacterial quantification techniques on different kinds of environmental samples and the effect of ultrasonic treatment}, series = {Journal of Microbiological Methods}, volume = {126}, journal = {Journal of Microbiological Methods}, issn = {0167-7012}, doi = {10.1016/j.mimet.2016.05.006}, pages = {42 -- 47}, abstract = {The precision of cell number quantification in environmental samples depends on the complexity of the sample and on the applied technique. We compared fluorescence microscopy after filtration, quantification of gene copies and the cultivation based most probable number technique for their precision. We further analyzed the effect of increasing complexity of the sample material on the precision of the different methods by using pure cultures of Pseudomonas aeruginosa, fresh water samples and sediment slurries with and without ultrasonic treatment for analyses. Microscopy reached the highest precision, which was similar between pure cultures and water samples, but lower for sediment samples due to a higher percentage of cells in clusters and flocks. The PCR based quantification was most precise for pure cultures. Water and sediment samples were similar but less precise, which might be caused by the applied DNA extraction techniques. MPN measurements were equally precise for pure cultures and water samples. For sediment slurries the precision was slightly lower. The applied ultrasonic treatment of the slurries dispersed the cell clusters and flocks, increased the precision of microscopical and MPN measurements and also increased the number of potential colony forming units. However, the culturable cell number decreased by half. For MPN quantification of viable cells in samples with a high proportion of clustered cells we therefore recommend an optimization of ultrasonic treatment and a confirmation by microscopy and cultivation to reach highest possible dispersion of the cells with a minimum of inactivation. As a result of these observations we suggest a correction factor for MPN measurements to consider the effect of sonication on complex samples. The results are most likely applicable to other complex samples such as soil or biofilms.}, language = {en} } @misc{BryantMartienssenBurkhardt, author = {Bryant, Isaac Mbir and Martienssen, Marion and Burkhardt, Marko}, title = {Millennium development goal on sustainable sanitation in Ghana: A reality or a mirage?}, series = {African Journal of science and research}, volume = {5}, journal = {African Journal of science and research}, number = {3}, issn = {2306-5877}, pages = {01 -- 06}, language = {en} } @misc{EngelbrechtFondengcapMartienssenetal., author = {Engelbrecht, Steffen and Fondengcap, Mbengamina Terence and Martienssen, Marion and Rathsack, Kristina}, title = {Highly efficient long-term storage of carrier-bound anammox biomass}, series = {Water Science \& Technology}, volume = {74}, journal = {Water Science \& Technology}, number = {8}, issn = {0273-1223}, doi = {10.2166/wst.2016.364}, pages = {1911 -- 1918}, abstract = {The anammox process is a potential alternative to the conventional nitrogen removal from wastewater. However, due to large generation times of anammox bacteria, the start-up of treatment reactors may be impeded. An efficient storage technique can handle this drawback and may be also suitable for seasonally operated treatment plants like in touristic areas. In the current study, several storage techniques were investigated with respect to its suitability for the preservation of the specific anammox activity after long-term storage. Storing conditions differed in terms of temperature, redox buffer and nutrient supplementation. The specific activity of immobilized anammox bacteria (Candidatus Kuenenia stuttgartiensis) was determined three times during a long-term preservation of 78 days and 106 days, respectively. The highest activity was ensured at a storing temperature of 4 WC, providing nitrate as redox buffer and a nutrient supplement every 23 days. Thus, 91.4\% of the initial anammox activity could be preserved after a storage of 106 days. Superiority of the presented treatment condition was confirmed by a calculated nitrate-ammonium consumption rate close to the optimal ratio of 1.32. This technique provided an economical and simple method suitable for long-term storage of immobilized anammox biomass.}, language = {en} } @misc{KuhnTothGeppertetal., author = {Kuhn, Ramona and T{\´o}th, Elisabeth and Geppert, Helmut and Fischer, Thomas and Martienssen, Marion}, title = {Identification of the Complete Photodegradation Pathway of Ethylenediaminetetra(methylenephosphonic acid) in Aqueous Solution}, series = {Clean - soil, air, water : a journal of sustainability and environmental safety}, volume = {45}, journal = {Clean - soil, air, water : a journal of sustainability and environmental safety}, number = {5}, issn = {1863-0669}, doi = {10.1002/clen.201500774}, pages = {1 -- 8}, abstract = {This study aims at investigating the abiotic degradation pathway of ethylenediaminetetra(methylenephosphonic acid) (EDTMP) simulated applying UV irradiation. The degradation of EDTMP and formation of degradation products was determined using LC-MS and ³¹P-NMR. In the laboratory scale experiments, EDTMP was degraded within 30 min and the degradation products, iminodi(methylenephosphonic acid) (IDMP), ethylaminobis(methylenephosphonic acid) (EABMP), and amino- (methylenephosphonic acid) (AMPA), were simultaneously released. IDMP was the main degradation product of EDTMP. Therefore, we conclude that the initial cleavage of EDTMP is a heterolytically driven process, which starts the degradation process at the intramolecular C-N bond. In contrast, the main product of a possible homolytic C-C cleavage of methylaminobis(methylenephosphonic acid) could not be confirmed with either LC-MS or ³¹P-NMR. Additionally, there was no evidence for a primary attack on the C-P bond. All identified degradation products of EDTMP have been mineralized to carbon dioxide (CO₂). Three additional degradation products (M1, M2, and M3) have been found using the ³¹P-NMR analysis but have not yet been quantified using LC-MS. We assume that the unidentified degradation product M1 is related to m/z 312, M2 to m/z 341, and M3 to m/z 409. Thus we concluded that EDTMP undergoes photochemical conversion to IDMP, the main degradation product. EABMP and AMPA also accumulate, but in smaller amounts. All intermediates are further mineralized to CO₂.}, language = {en} } @misc{KuhnBoellmannKrahletal., author = {Kuhn, Ramona and B{\"o}llmann, J{\"o}rg and Krahl, Kathrin and Bryant, Isaac Mbir and Martienssen, Marion}, title = {Comparison of ten different DNA extraction procedures with respect to their suitability for environmental samples}, series = {Journal of Microbiological Methods}, volume = {143}, journal = {Journal of Microbiological Methods}, issn = {0167-7012}, doi = {10.1016/j.mimet.2017.10.007}, pages = {78 -- 86}, abstract = {DNA extraction for molecular biological applications usually requires target optimized extraction procedures depending on the origin of the samples. For environmental samples, a range of different procedures has been developed. We compared the applicability and efficiency of ten selected DNA extraction methods published in recent literature using four different environmental samples namely: activated sludge from a domestic wastewater treatment plant, river sediment, anaerobic digestion sludge and nitrifying enrichment culture. We assessed the suitability of the extraction procedures based on both DNA yield and quality. DNA quantification was performed by both ultra violet (UV) spectrophotometry and fluorescence spectrophotometry after staining with PicoGreen. In our study, DNA yields based on UV measurement were overestimated in most cases while DNA yields from fluorescence measurements correlated well with the sample load on agarose gels of crude DNA. The quality of the DNA extracts was determined by gel electrophoresis of crude DNA and PCR products from 16S rDNA with the universal primer set 27f/1525r. It was observed that gel electrophoresis of crude DNA was not always suitable to evaluate DNA integrity and purity since interfering background substances (e.g. humic substances) were not visible. Therefore, we strongly recommend examining the DNA quality of both crude DNA and 16S rDNA PCR products by gel electrophoresis when a new extraction method is established. Summarizing, we found four out of ten extraction procedures being applicable to all tested samples without noticeable restrictions. The procedure G (according to the standard method 432_10401 of the Lower Saxony State Office for Consumer Protection and Food Safety) had the broadest application range over procedure J (published by Wilson, 2001). These were followed by procedures F (Singka et al., 2012) and A (Bourrain et al., 1999). All four extraction procedures delivered reliable and reproducible crude DNA and PCR products. From an economical point of view, all procedures tested during this study were cheaper compared to commercial DNA extraction kits.}, language = {en} } @misc{KuhnBoellmannKrahletal., author = {Kuhn, Ramona and B{\"o}llmann, J{\"o}rg and Krahl, Kathrin and Bryant, Isaac Mbir and Martienssen, Marion}, title = {Data on DNA gel sample load, gel electrophoresis, PCR and cost analysis}, series = {Data in Brief}, volume = {Vol. 16}, journal = {Data in Brief}, issn = {2352-3409}, doi = {10.1016/j.dib.2017.11.082}, pages = {732 -- 751}, abstract = {The data presented in this article provide supporting information to the related research article "Comparison of ten different DNA extraction procedures with respect to their suitability for environmental samples" (revised manuscript submitted to J. Microbiol. Methods). In that article, we compared the suitability of ten selected DNA extraction methods based on DNA quality, purity, quantity and applicability to universal PCR. Here we provide the data on the specific DNA gel sample load, all unreported gel images of crude DNA and PCR results, and the complete cost analysis for all tested extraction procedures and in addition two commercial DNA extraction kits for soil and water.}, language = {en} } @misc{DomańskaKuhnŁomotowskietal., author = {Domańska, Magdalena and Kuhn, Ramona and Łomotowski, Janusz and Stańczyk, Ewa}, title = {FISH method for identification of microbes in wastewater distribution systems}, series = {Environmental Protection Engineering}, volume = {40}, journal = {Environmental Protection Engineering}, number = {3}, issn = {0324-8828}, pages = {151 -- 160}, abstract = {The FISH (fluorescence in situ hybridization) method is widely used to identify various types of cells. In comparison to cultivation-dependent methods, identification of microbes by FISH is easier and generally takes several hours. This paper presents a review of improvements to the FISH method, its advantages and disadvantages, as well as examples of applications. Particular consideration was given to the efficiency of microbes identification in samples taken from sewerage. The effectiveness of the method was confirmed by the results obtained in samples from the membrane bioreactor (MBR).}, language = {en} } @misc{AdonadagaMartienssen, author = {Adonadaga, Melvin-Guy and Martienssen, Marion}, title = {In situ identification of filamentous beacteria from activated studge wastewater treatment plant in Ghana}, series = {Journal of Applied \& Environmental Microbiology}, volume = {3}, journal = {Journal of Applied \& Environmental Microbiology}, number = {3}, issn = {2373-6712}, pages = {75 -- 81}, language = {en} } @misc{AdonadagaMartienssen, author = {Adonadaga, Melvin-Guy and Martienssen, Marion}, title = {Reliability of morphological approach for bulking filamentous bacteria identification in activated sludge}, series = {International Journal of Applied Science and Technology}, volume = {6}, journal = {International Journal of Applied Science and Technology}, number = {2}, issn = {2221-0997}, pages = {30 -- 37}, language = {en} }