@misc{ZhiDrazanWolfbeisetal., author = {Zhi, Zheng-liang and Drazan, Viktor and Wolfbeis, Otto S. and Mirsky, Vladimir M.}, title = {Electrocatalytic activity of DNA on electrodes as an indication of hybridization}, series = {Bioelectrochemistry}, volume = {68}, journal = {Bioelectrochemistry}, number = {1}, issn = {1567-5394}, doi = {10.1016/j.bioelechem.2005.02.006}, pages = {1 -- 6}, abstract = {Electron transfer between metal electrodes and ferro/ferricyanide is completely suppressed at low ionic concentration. We describe here a new phenomenon related to this reaction: an immobilisation of thiolated single-stranded DNA on gold electrodes retains this activity at low ionic strength up to the level corresponding to the high ionic strength. In contrast, a hybridisation of the complementary DNAwith the thiolated single-stranded DNA followed by a binding onto the electrodes, attenuated the electrocatalytic effect. These effects can be used for discrimination between single-stranded DNA and double-stranded DNA and for semi-quantitative measurement of complementary DNA in a sample.}, language = {en} }