@misc{WitkabelAbendroth, author = {Witkabel, Philipp and Abendroth, Christian}, title = {A systematic literature review of microbial anammox consortia in UASB/ EGSB-reactors}, series = {Chemosphere}, volume = {367}, journal = {Chemosphere}, publisher = {Elsevier BV}, issn = {0045-6535}, doi = {10.1016/j.chemosphere.2024.143630}, pages = {17}, abstract = {Anaerobic ammonium oxidation (anammox) poses an emerging research field as it can outstand previous processes of biological wastewater treatment in terms of efficiency and costs. Anammox bacteria have the ability to metabolise NH4+ and NO2- to produce N2 under anaerobic conditions. Despite numerous studies, there is a lack of research on the co-occurrence and interrelationship of the predominant microbes that inhabit anammox-related processes. This systematic literature review follows the PSALSAR approach to assess metagenomic data on anammox bacteria and functional microbes in upstream reactors. Essential information on the physiology, metabolic pathways and inhibitory effects of anammox bacteria are reviewed and functional bacteria such as ammonia-oxidising bacteria (AOB), nitrite-oxidising bacteria (NOB), ammonia-oxidising Archaea (AOA) and denitrifying bacteria are identified. Candidatus Kuenenia and Candidatus Brocadia were the most frequently sequenced genera in the observed literature. Pseudomonadota, Chloroflexota and Bacteroidota were prevalent regardless of crucial operational parameters and configurations that affect the microbial community. Interrelationship analysis revealed a positive association between the versatility of a phylum's metabolism and its presence in the observed wastewater treatment literature. Several groups, such as Calditrichota, Myxococcota and Deinococcota are highly underrepresented, a finding that should be investigated in more detail. No evidence was found to suggest that high anammox ratios are correlated with high nitrogen removal efficiencies, as some studies found high efficiency despite low anammox abundance (<1\%).}, language = {en} } @misc{OttoWitkabelBarthetal., author = {Otto, Pascal and Witkabel, Philipp and Barth, Maximilian and Ammar, Aziz Ben and Rocktaeschel, Benjamin and Torrent, Daniel and Latorre-Per{\´e}z, Adriel and Krause, Maximilian and Abendroth, Christian}, title = {Adaptation of the anaerobic microbiome for in-situ power-to-CH4 processes through fuzzy logic control of H2 input}, series = {Bioresource technology reports}, volume = {31}, journal = {Bioresource technology reports}, publisher = {Elsevier BV}, address = {Amsterdam}, issn = {2589-014X}, doi = {10.1016/j.biteb.2025.102194}, pages = {1 -- 11}, abstract = {This study presents the first application of fuzzy logic control (FLC) for regulating in-situ biological methanation via H2 injection in a fixed-bed reactor. Given the role of CH4 as a storable and infrastructure-compatible energy carrier, the aim was to improve CH4 production by dynamically adapting the H2 supply to the metabolic capacity of the microbiome. This will pave the way for the biological utilisation and storage of renewable H2. Over a 226- day operational period, H2 input was gradually increased based on real-time headspace gas measurements. Coupling the gradual increase in H2 supply with real-time gas composition data allowed the microbiome to adapt dynamically to changing process conditions. This approach led to a 49 \% increase in CH4 concentration, from 58.4 \% to 87.0 \%, and improved H2 conversion rates from 98.2 \% to 99.0 \%. Chemical analyses of COD, organic acids and nitrogen were performed, as well as taxonomic analyses using 16S rRNA sequencing, and multivariate methods were applied to confirm the adaptation of the microbiome to the FLC H2 injection. The adopted microbial community was dominated by strictly hydrogenotrophic methanogens, Methanoculleus and Methanobacterium, while increased abundances of Petrimonas, Rectinema, Syntrophomonas, and Geobacter indicate interspecies H2 transfer and syntrophic cooperation enhancing methanogenesis. These findings demonstrate that FLC-based H2 control enables dynamic adjustment of in-situ methanation, optimising the interaction between hydrogenogenic, fermentative, syntrophic acetate-oxidising, and hydrogenotrophic microorganisms. This work introduces an adaptive control strategy that supports stable and efficient bio-CH4 production and represents a significant advance in the field of power-to-gas technologies.}, language = {en} }