@article{RaulienFriedrichStrobeletal., author = {Raulien, Nora and Friedrich, Kathleen and Strobel, Sarah and Raps, Stefanie and Hecker, Friederike and Pierer, Matthias and Schilling, Erik and Lainka, Elke and Kallinich, Tilmann and Baumann, Sven and Fritz-Wallace, Katarina and Rolle-Kampczyk, Ulrike and Bergen, Martin von and Aigner, Achim and Ewe, Alexander and Schett, Georg and Cross, Michael and Rossol, Manuela and Wagner, Ulf}, title = {Glucose-oxygen deprivation constrains HMGCR function and Rac1 prenylation and activates the NLRP3 inflammasome in human monocytes}, series = {Science Signaling}, volume = {17}, journal = {Science Signaling}, number = {845}, publisher = {American Association for the Advancement of Science (AAAS)}, issn = {1945-0877}, doi = {10.1126/scisignal.add8913}, pages = {19}, abstract = {Hypoxia and low glucose abundance often occur simultaneously at sites of inflammation. In monocytes and macrophages, glucose-oxygen deprivation stimulates the assembly of the NLRP3 inflammasome to generate the proinflammatory cytokine IL-1β. We found that concomitant glucose deprivation and hypoxia activated the NLRP3 inflammasome by constraining the function of HMG-CoA reductase (HMGCR), the rate-limiting enzyme of the mevalonate kinase pathway. HMGCR is involved in the synthesis of geranylgeranyl pyrophosphate (GGPP), which is required for the prenylation and lipid membrane integration of proteins. Under glucose-oxygen deprivation, GGPP synthesis was decreased, leading to reduced prenylation of the small GTPase Rac1, increased binding of nonprenylated Rac1 to the scaffolding protein IQGAP1, and enhanced activation of the NLRP3 inflammasome. In response to restricted oxygen and glucose supply, patient monocytes with a compromised mevalonate pathway due to mevalonate kinase deficiency or Muckle-Wells syndrome released more IL-1β than did control monocytes. Thus, reduced GGPP synthesis due to inhibition of HMGCR under glucose-oxygen deprivation results in proinflammatory innate responses, which are normally kept in check by the prenylation of Rac1. We suggest that this mechanism is also active in inflammatory autoimmune conditions.}, language = {en} } @misc{JahnkeCruysenPrilletal., author = {Jahnke, Sonja and Cruysen, Caren and Prill, Robert and Kittmann, Fabian and Pflug, Nicola and Albert, Justin Amadeus and Camargo, Tibor de and Arnrich, Bert and Krolikowska, Aleksandra and Kolcz, Anna and Reichert, Pawel and Oleksy, Lukasz and Michel, Sven and Kopf, Sebastian and Wagner, Michael and Scheffler, Sven and Becker, Roland}, title = {Protocol for a Randomized Crossover Trial to Evaluate the Effect of Soft Brace and Rigid Orthosis on Performance and Readiness to Return to Sport Six Months Post‑ACL‑Reconstruction}, series = {Healthcare}, volume = {11}, journal = {Healthcare}, number = {4}, issn = {2227-9032}, doi = {10.3390/healthcare11040513}, abstract = {A randomized crossover trial was designed to investigate the influence of muscle activation and strength on functional stability/control of the knee joint, to determine whether bilateral imbalances still occur six months after successful anterior cruciate ligament reconstruction (ACLR), and to analyze whether the use of orthotic devices changes the activity onset of these muscles. Furthermore, conclusions on the feedforward and feedback mechanisms are highlighted. Therefore, twenty‑eight patients will take part in a modified Back in Action (BIA) test battery at an average of six months after a primary unilateral ACLR, which used an autologous ipsilateral semitendinosus tendon graft. This includes double‑leg and single‑leg stability tests, double‑leg and single‑leg countermovement jumps, double‑leg and single‑leg drop jumps, a speedy jump test, and a quick feet test. During the tests, gluteus medius and semitendinosus muscle activity are analyzed using surface electromyography (sEMG). Motion analysis is conducted using Microsoft Azure DK and 3D force plates. The tests are performed while wearing knee rigid orthosis, soft brace, and with no aid, in random order. Additionally, the range of hip and knee motion and hip abductor muscle strength under isometric conditions are measured. Furthermore, patient‑rated outcomes will be assessed.}, language = {en} } @misc{DropcoKaiserWagneretal., author = {Dropco, Ivor and Kaiser, Ulrich and Wagner, Lola and Brunner, Stefan M. and Schlitt, Hans J{\"u}rgen and Stroszczynski, Christian and Jung, Friedrich and Yi, Dong and Herr, Wolfgang and Jung, Ernst Michael}, title = {Color mapping using ultrasound system-integrated perfusion software for evaluation of focal liver lesions: a possible first step for more independent reading}, series = {Journal of Gastrointestinal and Liver Diseases}, volume = {32}, journal = {Journal of Gastrointestinal and Liver Diseases}, number = {4}, issn = {1842-1121}, doi = {10.15403/jgld-4997}, pages = {479 -- 487}, abstract = {Aims To assess the value of using integrated parametric ultrasound software for contrast-enhanced ultrasonography (CEUS) of liver tumors. Methods 107 patients with liver tumors were studied. CEUS were performed to detect focal lesions. Parametric images were based on continuous CINE LOOPs, from the early-arterial phase (15 s) to the portal-venous phase (1 min) generated by perfusion software. The evaluations of the parametric images and their dignity for liver lesions were performed independently by an experienced and a less-experienced investigator. Computed tomography, magnetic resonance imaging scans or histological analysis were used as references. Results High parametric image quality were obtained in all patients. Among the patients, 44\% lesions were benign, 56\% were malignant. The experienced investigator correctly classified 46 of 47 (98\%) as benign, and 60 of 60 (100\%) as malignant tumors based on the parametric images. The less-experienced investigator correctly classified 39 of 47 (83\%) as benign, and 49 of 60 (82\%) malignant tumors, acheaving a high statistical accuracy of 98\% with this type of diagnostic. Conclusion Parametric imaging for grading the malignant degree of tumor may be a good complement to existing ultrasound techniques and was particularly helpful for improving the assessments of the less-experienced examiner.}, language = {en} } @misc{LuetznerLangeSchmittetal., author = {L{\"u}tzner, J{\"o}rg and Lange, Toni and Schmitt, Jochen and Kopkow, Christian and Dreinh{\"o}fer, Karsten E and Friederich, Niklaus F. and Gravius, Sascha and Heller, Karl-Dieter and Hube, Robert and Gromnica-Ihle, Erika and Kirschner, Stephan and Kladny, Bernd and Kremer, Michael and Linke, Maike and Malzahn, J{\"u}rgen and Sabatowski, Rainer and Scharf, Hanns-Peter and St{\"o}ve, Johannes and Wagner, Richard and G{\"u}nther, Klaus-Peter}, title = {[The S2k guideline: Indications for knee endoprosthesis : Evidence and consent-based indications for total knee arthroplasty]}, series = {Der Orthop{\"a}de}, volume = {47}, journal = {Der Orthop{\"a}de}, number = {9}, issn = {1433-0431}, doi = {10.1007/s00132-018-3612-x}, pages = {777 -- 781}, language = {en} }