@misc{HerzogHansenMiethbaueretal., author = {Herzog, Natalie and Hansen, Max and Miethbauer, Sebastian and Schmidtke, Kai-Uwe and Anderer, Ursula and Lupp, Amelie and Sperling, Sebastian and Seehofer, Daniel and Damm, Georg and Scheibner, Katrin and K{\"u}pper, Jan-Heiner}, title = {Primary-like human hepatocytes genetically engineered to obtain proliferation competence display hepatic differentiation characteristics in monolayer and organotypical spheroid cultures}, series = {Cell Biology International}, volume = {40}, journal = {Cell Biology International}, number = {3}, issn = {1095-8355}, doi = {10.1002/cbin.10574}, pages = {341 -- 353}, abstract = {Primary human hepatocytes are in great demand during drug development and in hepatology. However, both scarcity of tissue supply and donor variability of primary cells create a need for the development of alternative hepatocyte systems. By using a lentivirus vector system to transfer coding sequences of Upcyte® proliferation genes, we generated non-transformed stable hepatocyte cultures from human liver tissue samples. Here, we show data on newly generated proliferation-competent HepaFH3 cells investigated as conventional two-dimensional monolayer and as organotypical three-dimensional (3D) spheroid culture. In monolayer culture, HepaFH3 cells show typical cobblestone-like hepatocyte morphology and anchorage-dependent growth for at least 20 passages. Immunofluorescence staining revealed that characteristic hepatocyte marker proteins cytokeratin 8, human serum albumin, and cytochrome P450 (CYP) 3A4 were expressed. Quantitative real-time PCR analyses showed that expression levels of analyzed phase I CYP enzymes were at similar levels compared to those of cultured primary human hepatocytes and considerably higher than in the liver carcinoma cell line HepG2. Additionally, transcripts for phase II liver enzymes and transporter proteins OATP-C, MRP2, Oct1, and BSEP were present in HepaFH3. The cells produced urea and converted model compounds such as testosterone, diclofenac, and 7-OH-coumarin into phases I and II metabolites. Interestingly, phases I and II enzymes were expressed at about the same levels in convenient monolayer cultures and complex 3D spheroids. In conclusion, HepaFH3 cells and related primary-like hepatocyte lines seem to be promising tools for in vitro research of liver functions and as test system in drug development and toxicology analysis.}, language = {en} } @misc{HerzogHansenMiethbaueretal., author = {Herzog, Natalie and Hansen, Max and Miethbauer, Sebastian and Schmidtke, Kai-Uwe and Anderer, Ursula and Lupp, Amelie and Sperling, Sebastian and Seehofer, Daniel and Damm, Georg and Scheibner, Katrin and K{\"u}pper, Jan-Heiner}, title = {Primary like human hepatocytes genetically engineered to obtain proliferation competence display liver biotransformation activity in 2D and 3D culture systems}, series = {Cell Biology International}, volume = {40}, journal = {Cell Biology International}, number = {3}, issn = {1095-8355}, pages = {341 -- 353}, language = {en} } @misc{LehmannMartinMannigeletal., author = {Lehmann, Mario and Martin, Frank and Mannigel, Kerstin and Kaltschmidt, Kristin and Sack, Ulrich and Anderer, Ursula}, title = {Three-dimensional scaffold-free fusion culture: the way to enhanced chondrogenesis of in vitro propagated human articular chondrocytes}, series = {European Journal of Histochemistry}, volume = {57}, journal = {European Journal of Histochemistry}, number = {4}, issn = {2038-8306}, doi = {10.4081/ejh.2013.e31}, pages = {205 -- 215}, language = {en} } @misc{MartinLehmannSchlaegeretal., author = {Martin, Frank and Lehmann, Mario and Schl{\"a}ger, Peter and Sack, Ulrich and Anderer, Ursula}, title = {Differentiation capacity of chondrocytes in microtissues depends on TGF-ß subtyp}, series = {Journal of biochips \& tissue chips : JBTC}, volume = {S2}, journal = {Journal of biochips \& tissue chips : JBTC}, number = {002}, issn = {2153-0777}, doi = {10.4172/2153-0777.S2-002}, pages = {11}, language = {en} } @misc{KnuetterHiemannBrummaetal., author = {Kn{\"u}tter, Ilka and Hiemann, Rico and Brumma, Therese and B{\"u}ttner, Thomas and Großmann, Kai and Cusini, Marco and Pregnolato, Francesca and Borghi, Maria Orietta and Anderer, Ursula and Conrad, Karsten and Reinhold, Dirk and Roggenbuck, Dirk and Csernok, Elena}, title = {Automated interpretation of ANCA patterns - a new approach in the serology of ANCA-associated vasculitis}, series = {Arthritis Research \& Therapy}, volume = {14}, journal = {Arthritis Research \& Therapy}, issn = {1465-9913}, doi = {10.1186/ar4119}, pages = {10}, language = {en} } @misc{LutterScholkaRichteretal., author = {Lutter, Anne-Helen and Scholka, Jenny and Richter, Heiko and Anderer, Ursula}, title = {Applying XTT, WST-1, and WST-8 to human chondrocytes: A comparison of membrane-impermeable tetrazolium salts in 2D and 3D cultures}, series = {Clinical Hemorheology and Microcirculation}, volume = {67}, journal = {Clinical Hemorheology and Microcirculation}, number = {3-4}, issn = {1875-8622}, doi = {10.3233/CH-179213}, pages = {327 -- 342}, language = {en} } @misc{LutterHempelAndereretal., author = {Lutter, Anne-Helen and Hempel, Ute and Anderer, Ursula and Dieter, Peter}, title = {Biphasic influence of PGE2 on the resorption activity of osteoclast-like cells derived from human peripheral blood monocytes and mouse RAW264.7}, series = {Prostaglandins, Leukotrienes and Essential Fatty Acids}, volume = {111}, journal = {Prostaglandins, Leukotrienes and Essential Fatty Acids}, issn = {1532-2823}, doi = {10.1016/j.plefa.2016.03.017}, pages = {1 -- 7}, language = {en} } @misc{MartinLehmannSacketal., author = {Martin, Frank and Lehmann, Mario and Sack, Ulrich and Anderer, Ursula}, title = {In vitro development of personalized cartilage microtissues uncovers an individualized differentiation capacity of human chondrocytes}, series = {Experimental Biology and Medicine}, volume = {242}, journal = {Experimental Biology and Medicine}, number = {18}, issn = {1535-3702}, doi = {10.1177/1535370217728498}, pages = {1746 -- 1756}, language = {en} } @incollection{AndererSavkovic, author = {Anderer, Ursula and Savkovic, Vuc}, title = {Zelltherapien in der Regenerativen Medizin}, series = {Zellul{\"a}re Diagnostik und Therapie}, booktitle = {Zellul{\"a}re Diagnostik und Therapie}, editor = {Sack, Ulrich}, edition = {1. Auflage}, publisher = {Walter de Gruyter}, address = {Berlin [u.a.]}, isbn = {978-3-11-034406-6}, pages = {291 -- 341}, language = {de} } @incollection{MartinLehmannAnderer, author = {Martin, Frank and Lehmann, Mario and Anderer, Ursula}, title = {Generation of scaffold free 3-D cartilage-like microtissues from human chondrocytes}, series = {Medical Advancements in Aging and Regenerative Technologies: Clinical Tools and Applications}, booktitle = {Medical Advancements in Aging and Regenerative Technologies: Clinical Tools and Applications}, editor = {Daskalaki, Adriani}, publisher = {IGI Global}, address = {Hershey, PA, USA}, isbn = {978-1-4666-2506-8}, pages = {169 -- 194}, language = {en} } @misc{LutterLiedtkeEckeetal., author = {Lutter, Anne-Helen and Liedtke, Victoria and Ecke, Annemarie and Scholka, Jenny and Muschter, Antje and Hentschel, Erik and Becker, Roland and Anderer, Ursula}, title = {Direct co-culture of human chondrogenic microtissues with osteoblast-like Saos-2 cells positively influence the differentiation potential of cartilage cells in 3D culture}, doi = {10.1007\%2Fs10354-018-0664-9.pdf}, pages = {S. 51}, language = {en} } @misc{LutterMuschterAnderer, author = {Lutter, Anne-Helen and Muschter, Antje and Anderer, Ursula}, title = {Diagnostic Test System to detect resorption activity of osteoclasts in vitro just using small blood samples}, series = {Osteologie}, volume = {28}, journal = {Osteologie}, number = {4}, publisher = {Georg Thieme Verlag KG}, address = {Stuttgart ; New York}, issn = {2567-5818}, doi = {10.1055/s-0039-1680038}, pages = {72}, language = {en} } @misc{EckeScholkaHanschetal., author = {Ecke, Annemarie and Scholka, Jenny and Hansch, Anna and Becker, Roland and Anderer, Ursula}, title = {Agar overlay technique vs. v-bottom plate: differentiation of human cartilage-like microtissues depend on plate format.}, series = {Osteologie}, volume = {28}, journal = {Osteologie}, number = {4}, publisher = {Georg Thieme Verlag KG}, address = {Stuttgart ; New York}, issn = {2567-5818}, doi = {10.1055/s-0039-1680058}, pages = {81}, language = {en} } @misc{LutterEckeScholkaetal., author = {Lutter, Anne-Helen and Ecke, Annemarie and Scholka, Jenny and Waldeck, Peter and Tretter, Tim and Anderer, Ursula}, title = {Differentiation of human chondrocytes under the influence of microalgae-based exopolysaccharides}, series = {38. Jahrestagung der Deutschen Gesellschaft f{\"u}r Klinische Mikrozirkulation und H{\"a}morheologie : Programm, 22. und 23. November 2019, Braunschweig}, journal = {38. Jahrestagung der Deutschen Gesellschaft f{\"u}r Klinische Mikrozirkulation und H{\"a}morheologie : Programm, 22. und 23. November 2019, Braunschweig}, abstract = {BACKGROUND: Exopolysaccharides are formed by microalgae in response to adverse conditions and are found mainly as a coating around the cells. The protective function of EPS plays an important role in the survival of microalgae in polluted habitats. So far, the particular biological and physico-chemical properties of EPS have not been adequately investigated and their effect on cells such as human chondrocytes has not been tested. Microalgae in particular offer an ideal platform for the production of dietary supplements or drugs that enable new therapeutic approaches. METHODS: To investigate the influence of EPS on human chondrocytes, cytotoxicity and differentiation potential were analyzed in 3D cultures. RESULTS: We were able to demonstrate a positive, but donor-specific influence of EPS on the differentiation of human chondrocytes. Furthermore, the EPS showed no cytotoxic effects in a range of up to 250 µg EPS per ml medium. CONCLUSION: EPS have the potential to positively influence the differentiation of human chondrocytes. Further experiments are necessary to analyze the donor-specific effect.}, language = {en} } @misc{EckeScholkaHanschetal., author = {Ecke, Annemarie and Scholka, Jenny and Hansch, Anna and Becker, Roland and Anderer, Ursula}, title = {Differentiation of human articular chondrocytes in microtissues depends on the cell aggregation technique}, pages = {1}, language = {en} } @inproceedings{LehmannMartinScholkaetal., author = {Lehmann, Mario and Martin, Frank and Scholka, Jenny and Petto, Carola and Wittenburg, Gretel and Sack, Ulrich and Anderer, Ursula}, title = {Human chondrocytes and dental pulp stem cells enhance their chondrogenic differentiation potential via a two-step fusion culture technique}, series = {Tissue Engineering, Part A, Vol. 21, Suppl. 1}, booktitle = {Tissue Engineering, Part A, Vol. 21, Suppl. 1}, doi = {10.1089/ten.tea.2015.5000.abstracts}, pages = {S-268 -- S-269}, language = {en} } @inproceedings{LutterHempelDieteretal., author = {Lutter, Anne-Helen and Hempel, Ute and Dieter, Peter and Anderer, Ursula}, title = {Cost-efficient diagnostic platform to measure osteoclast activity}, series = {35. Jahrestagung der Deutschen Gesellschaft f{\"u}r Klinische Mikrozirkulation und H{\"a}morheologie, Mainz, 2016}, booktitle = {35. Jahrestagung der Deutschen Gesellschaft f{\"u}r Klinische Mikrozirkulation und H{\"a}morheologie, Mainz, 2016}, doi = {10.13140/RG.2.2.10550.19528}, language = {en} } @inproceedings{LehmannVossMartinetal., author = {Lehmann, Mario and Voß, Fr{\"a}nzi and Martin, Frank and Schl{\"a}ger, Peter and Anderer, Ursula}, title = {Chondrogenic microtissues in the lab \& for the clinic}, series = {3D cell culture 2016, book of abstracts, DECHEMA Biotechnologie}, booktitle = {3D cell culture 2016, book of abstracts, DECHEMA Biotechnologie}, publisher = {DECHEMA e.V.}, address = {Frankfurt am Main}, isbn = {978-3-89746-182-6}, pages = {52 -- 53}, language = {en} } @inproceedings{LehmannVossMartinetal., author = {Lehmann, Mario and Voß, Fr{\"a}nzi and Martin, Frank and Richter, Heiko and Anderer, Ursula}, title = {Chondrogenic microtissues with a lower differentiation degree used as in vitro transplants show improved integration in the tissue repair process in an in vitro therapy culture system}, series = {Regenerative Medicine}, volume = {10}, booktitle = {Regenerative Medicine}, number = {7S}, issn = {1746-0751}, pages = {S. 53}, language = {en} } @inproceedings{LehmannScholkaWittenburgetal., author = {Lehmann, Mario and Scholka, Jenny and Wittenburg, Gretel and Petto, Carola and Geppert, Philipp and Muschter, Antje and Anderer, Ursula}, title = {An easily accessible adult stem cell pool may be suitable for tissue engineering-based regeneration of articular cartilage defects}, series = {eCM Conferences Open Access online periodical}, booktitle = {eCM Conferences Open Access online periodical}, isbn = {2522-235X}, pages = {P190}, language = {en} }