@misc{KarichCaiLinhardtetal., author = {Karich, Alexander and Cai, Hongtao and Linhardt, Anne and Antony, Anila and Liers, Christiane and Ullrich, Ren{\´e} and Schwaderer, Fabian and Kalmbach, Johannes and Scheibner, Katrin and Hofrichter, Martin and Synytska, Alla}, title = {Conductive hairy particles with homogeneous and Janus design as carrier materials for the efficient immobilization of unspecific peroxygenases}, series = {Biotechnology journal : systems \& synthetic biology, nanobiotech, medicine}, volume = {20}, journal = {Biotechnology journal : systems \& synthetic biology, nanobiotech, medicine}, number = {7}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1860-7314}, doi = {10.1002/biot.70078}, pages = {1 -- 12}, abstract = {Efficient immobilization of unspecific peroxygenases (UPOs) on hairy particles with homogeneous and Janus design possessingconductive core and polymeric shell was demonstrated. PDMAEMA brushes (hairs) with controlled chain lengths weresuccessfully grown from the conductive silver particles allowing further immobilization of enzymes and keeping their activity.The Janus design of the synthesized particles maintained the conductivity of the core material. Enzyme immobilization onbrush-modified particles was first carried out with two laccases as model enzymes [Trametes versicolor (TveLac) and Pycnoporuscinnabarinus (PciLac)] and then successfully extended to UPOs [two wild-type and one recombinant UPO from Marasmius rotula(MroUPO) and Agrocybe aegerita (AaeUPO, rAaeUPO)]. The most efficient immobilization was achieved for MroUPO on Janusparticles. The enzyme loading is reversible and could be successfully repeated after cleaning of the particles. Thus, the successfulimmobilization of an MroUPO on conductive hairy Janus particles was demonstrated for the first time. The major advantage ofthe proposed approach lies in the reusability of the enzyme and its carrier, as well as in the use of conductive core materials, whichcould be promising, for example, as material for electrochemical biosensors in future.}, language = {en} }