@incollection{StoellnerBreitenlechnerEibneretal., author = {St{\"o}llner, Thomas and Breitenlechner, Elisabeth and Eibner, Clemens and Herd, Rainer and Kienlin, Tobias and Lutz, Joachim and Maass, Alexander and Nicolussi, Kurt and Pichler, Thomas and Pils, Robert and R{\"o}ttger, Klaus and Song, Baoquan and Taube, Nadine and Thomas, Peter and Thurner, Andrea}, title = {Der Mitterberg - der Großproduzent f{\"u}r Kupfer im {\"o}stlichen Alpenraum w{\"a}hrend der Bronzezeit}, series = {Forschungsprogramm HiMAT - Neues zur Bergbaugeschichte der Ostalpen}, booktitle = {Forschungsprogramm HiMAT - Neues zur Bergbaugeschichte der Ostalpen}, editor = {Goldenberg, Gert and T{\"o}chterle, Ulrike and Oeggl, Klaus and Krenn-Leeb, Alexandra}, publisher = {{\"O}sterreichische Gesellschaft f{\"u}r Ur- und Fr{\"u}hgeschichte}, address = {Wien}, isbn = {978-3-902572-03-5}, pages = {113 -- 144}, language = {de} } @misc{KammererJahnWinteretal., author = {Kammerer, Sarah and Jahn, Stephan Wenzel and Winter, Elke and Eidenhammer, Sylvia and Rezania, Simin and Regitnig, Peter and Pichler, Martin and Schreibmayer, Wolfgang and Bauernhofer, Thomas}, title = {Critical evaluation of KCNJ3 gene product detection in human breast cancer: MRNA in situ hybridisation is superior to immunohistochemistry}, series = {Journal of Clinical Pathology}, volume = {69}, journal = {Journal of Clinical Pathology}, number = {12}, issn = {1472-4146}, doi = {10.1136/jclinpath-2016-203798}, pages = {1116 -- 1121}, abstract = {Increased expression levels of KCNJ3 have been correlated with lymph node metastases and poor prognosis in patients with breast cancer, suggesting a prognostic role of KCNJ3. We aimed to establish protocols for the detection of KCNJ3 in formalin-fixed, paraffin-embedded (FFPE) breast cancer tissue. Several antibodies were tested for sensitivity and specificity by western blot, followed by optimisation of the immunohistochemistry (IHC) procedure and establishment of KCNJ3 mRNA in situ hybridisation (ISH). Methods were validated by processing 15 FFPE breast cancer samples for which microarray data were available. Spearman's rank correlation analysis resulted in borderline significant correlation for IHC versus ISH (rS: 0.625; p<0.05) and IHC versus microarray (rS: 0.668; p<0.01), but in significant correlation for ISH versus microarray (rS: 0.861; p<0.001). The ISH method was superior to IHC, regarding robustness, sensitivity and specificity and will aid to further study expression levels of KCNJ3 in both malignant and physiological conditions.}, language = {en} } @misc{KammererSokolowskiHackletal., author = {Kammerer, Sarah and Sokolowski, Armin Andreas and Hackl, Hubert and Platzer, Dieter and Jahn, Stephan Wenzel and El-Heliebi, Amin and Schwarzenbacher, Daniela and Stiegelbauer, Verena and Pichler, Martin and Rezania, Simin and Fiegl, Heidelinde and Peintinger, Florentia and Regitnig, Peter and H{\"o}fler, Gerald and Schreibmayer, Wolfgang and Bauernhofer, Thomas}, title = {KCNJ3 is a new independent prognostic marker for estrogen receptor positive breast cancer patients}, series = {OncoTarget : open access impact journal}, volume = {7}, journal = {OncoTarget : open access impact journal}, number = {51}, doi = {10.18632/oncotarget.13224}, pages = {84705 -- 84717}, abstract = {Numerous studies showed abnormal expression of ion channels in different cancer types. Amongst these, the potassium channel gene KCNJ3 (encoding for GIRK1 proteins) has been reported to be upregulated in tumors of patients with breast cancer and to correlate with positive lymph node status. We aimed to study KCNJ3 levels in different breast cancer subtypes using gene expression data from the TCGA, to validate our findings using RNA in situ hybridization in a validation cohort (GEO ID GSE17705), and to study the prognostic value of KCNJ3using survival analysis. In a total of > 1000 breast cancer patients of two independent data sets we showed a) that KCNJ3 expression is upregulated in tumor tissue compared to corresponding normal tissue (p < 0.001), b) that KCNJ3 expression is associated with estrogen receptor (ER) positive tumors (p < 0.001), but that KCNJ3 expression is variable within this group, and c) that ER positive patients with high KCNJ3 levels have worse overall (p < 0.05) and disease free survival probabilities (p < 0.01), whereby KCNJ3 is an independent prognostic factor (p <0.05). In conclusion, our data suggest that patients with ER positive breast cancer might be stratified into high risk and low risk groups based on the KCNJ3 levels in the tumor.}, language = {en} }