@misc{RezaniaKammererLietal., author = {Rezania, Simin and Kammerer, Sarah and Li, Chouyang and Steinecker-Frohnwieser, Bibiane and Gorischek, Astrid and Devaney, Trevor Thomas Joseph and Verheyen, Sarah and Passegger, Christina and Ghaffari Tabrizi-Wizsy, Nassim and Hackl, Hubert and Platzer, Dieter and Zarnani, A.H. and Malle, Ernst and Jahn, Stephan Wenzel and Bauernhofer, Thomas and Schreibmayer, Wolfgang}, title = {Overexpression of KCNJ3 gene splice variants affects vital parameters of the malignant breast cancer cell line MCF-7 in an opposing manner}, series = {BMC Cancer}, volume = {16}, journal = {BMC Cancer}, issn = {1471-2407}, doi = {10.1186/s12885-016-2664-8}, abstract = {Background Overexpression the KCNJ3, a gene that encodes subunit 1 of G-protein activated inwardly rectifying K+ channel (GIRK1) in the primary tumor has been found to be associated with reduced survival times and increased lymph node metastasis in breast cancer patients. Methods In order to survey possible tumorigenic properties of GIRK1 overexpression, a range of malignant mammary epithelial cells, based on the MCF-7 cell line that permanently overexpress different splice variants of the KCNJ3 gene (GIRK1a, GIRK1c, GIRK1d and as a control, eYFP) were produced. Subsequently, selected cardinal neoplasia associated cellular parameters were assessed and compared. ResultsAdhesion to fibronectin coated surface as well as cell proliferation remained unaffected. Other vital parameters intimately linked to malignancy, i.e. wound healing, chemoinvasion, cellular velocities / motilities and angiogenesis were massively affected by GIRK1 overexpression. Overexpression of different GIRK1 splice variants exerted differential actions. While GIRK1a and GIRK1c overexpression reinforced the affected parameters towards malignancy, overexpression of GIRK1d resulted in the opposite. Single channel recording using the patch clamp technique revealed functional GIRK channels in the plasma membrane of MCF-7 cells albeit at very low frequency. DiscussionWe conclude that GIRK1d acts as a dominant negative constituent of functional GIRK complexes present in the plasma membrane of MCF-7 cells, while overexpression of GIRK1a and GIRK1c augmented their activity. The core component responsible for the cancerogenic action of GIRK1 is apparently presented by a segment comprising aminoacids 235-402, that is present exclusively in GIRK1a and GIRK1c, but not GIRK1d (positions according to GIRK1a primary structure). Conclusions The current study provides insight into the cellular and molecular consequences of KCNJ3 overexpression in breast cancer cells and the mechanism upon clinical outcome in patients suffering from breast cancer.}, language = {en} } @inproceedings{SimonMeissnerWichmannetal., author = {Simon, Sylvio and Meißner, Thomas and Wichmann, Steffen and Hernschier, Stephan and Weist, Michael}, title = {Energieeffizienz in der Maschinenkonstruktion}, series = {Proceedings of the International Academic Forum AMO-SPITSE-NESEFF, 21 AMO Conference, Smolensk 2016}, booktitle = {Proceedings of the International Academic Forum AMO-SPITSE-NESEFF, 21 AMO Conference, Smolensk 2016}, publisher = {Izdat. Universum}, address = {Moscow [u.a.]}, isbn = {978-5-91412-313-7}, pages = {S. 125}, abstract = {Die konsequente Anwendung von Gestaltungsrichtlinien im Bezug auf die Masse der verwendeten Maschinenelemente hilft energieeffiziente Produkte zu gestalten. Maschinenelemente wie Schrauben, ber{\"u}hrungslose Dichtungen oder Radialwellendichtringe k{\"o}nnen dabei einen entsprechenden Beitrag leisten.}, language = {de} } @inproceedings{HernschierRiederSchneideretal., author = {Hernschier, Stephan and Rieder, Thomas and Schneider, Robert and Magister, Jan and Simon, Sylvio}, title = {Aktuelle Untersuchungen an Tragrollen}, series = {3. Ingenieurtag 2016 der Fakult{\"a}t Maschinenbau, Elektro- und Energiesysteme - GUS und Osteuropatag - NESEFF-Netzwerktreffen 2016, Tagungsband}, booktitle = {3. Ingenieurtag 2016 der Fakult{\"a}t Maschinenbau, Elektro- und Energiesysteme - GUS und Osteuropatag - NESEFF-Netzwerktreffen 2016, Tagungsband}, editor = {Simon, Sylvio}, publisher = {Brandenburgische Techn. Univ.}, address = {Cottbus-Senftenberg}, isbn = {978-3-940471-28-4}, pages = {157 -- 161}, abstract = {Die Minderung der Ger{\"a}uschemission von Hochleistungsgurtf{\"o}rderanlagen in Tagebauen stellt in Verbindung mit h{\"o}heren Anforderungen des Umweltschutzes und der Akzeptanz in der Bev{\"o}lkerung ein Problem dar. Zum einen gilt es Mensch und Umwelt vor L{\"a}rm zu sch{\"u}tzen und zum anderen ist es f{\"u}r den Betreiber schwierig, die gesetzlichen Auflagen f{\"u}r den Betrieb solcher Anlagen zu erf{\"u}llen. Untersuchungen haben gezeigt, dass Tragrollen die Hauptschallquellen dieser Gurtf{\"o}rderanlagen sind. Im Rahmen einer Baugruppenpr{\"u}fung werden in Kooperation mit der Lausitz Energie Bergbau AG (LEAG) akustische und mechanische Kenngr{\"o}ßen von Tragrollen auf einem Pr{\"u}fstand (TPS) an der Brandenburgischen Technischen Universit{\"a}t Cottbus-Senftenberg untersucht.}, language = {de} } @inproceedings{RoesslerRoggeHentschel, author = {R{\"o}ßler, Thomas and Rogge, Stephan and Hentschel, Christian}, title = {Optimized light output for passive stereoscopic projection in VR-systems}, series = {IEEE 6th International Conference on Consumer Electronics - Berlin (ICCE-Berlin), 5-7 Sept. 2016}, booktitle = {IEEE 6th International Conference on Consumer Electronics - Berlin (ICCE-Berlin), 5-7 Sept. 2016}, publisher = {IEEE}, address = {Piscataway, NJ}, isbn = {978-1-5090-2096-6}, doi = {10.1109/ICCE-Berlin.2016.7684769}, pages = {5}, language = {en} } @inproceedings{RepinsMansfieldKanevceetal., author = {Repins, Ingrid and Mansfield, Lorelle M. and Kanevce, Ana and Jensen, Soren A. and Kuciauskas, Darius and Glynn, Stephen and Barnes, Teresa and Metzger, Wyatt and Burst, James and Jiang, Chun-Sheng and Harvey, Steve and Dippo, Patricia and Teeter, Glenn and Perkins, C. and Egaas, Brian and Zakutayev, Andriy and Alsmeier, Jan Hendrik and Lußky, Thomas and Korte, Lars and Wilks, Regan G. and B{\"a}r, Marcus and Yan, Yanfa and Lany, Stephan and Zawadzki, Pawel and Zawadzki, Pawel and Park, Ji-Sang and Wei, Suhuai}, title = {Wild band edges: The role of bandgap grading and band-edge fluctuations in high-efficiency chalcogenide devices}, series = {2016 IEEE 43rd Photovoltaic Specialists Conference (PVSC), Portland, OR, USA, June 5-10, 2016}, booktitle = {2016 IEEE 43rd Photovoltaic Specialists Conference (PVSC), Portland, OR, USA, June 5-10, 2016}, publisher = {IEEE}, address = {Piscataway, NJ}, isbn = {978-1-5090-2724-8}, pages = {309 -- 314}, language = {en} } @inproceedings{HandickReinhardWilksetal., author = {Handick, Evelyn and Reinhard, Patrick and Wilks, Regan G. and Pianezzi, Fabian and Duarte, Roberto Felix and Gorgoi, Mihaela and Kunze, Thomas and B{\"u}cheler, Stephan and Tiwari, Ayodhya N. and B{\"a}r, Marcus}, title = {NaF/KF post-deposition treatments and their influence on the structure of Cu(In,Ga)Se₂ absorber surfaces}, series = {IEEE 43rd Photovoltaic Specialists Conference (PVSC), 5-10 June 2016}, booktitle = {IEEE 43rd Photovoltaic Specialists Conference (PVSC), 5-10 June 2016}, publisher = {IEEE}, address = {Piscataway, NJ}, isbn = {978-1-5090-2724-8}, doi = {10.1109/PVSC.2016.7749400}, pages = {17 -- 21}, language = {en} } @inproceedings{BergerSchaeferSchottkeetal., author = {Berger, Ulrich and Sch{\"a}fer, Stephan and Schottke, Dirk and Kampfe, Thomas and Tauber, Bernd and Kr{\"o}nke, Dietrich}, title = {Increasing the flexibility of manufacturing: A service-oriented approach in automation}, series = {21st International Conference on Emerging Technologies and Factory Automation (ETFA), 6-9 Sept. 2016, Berlin}, booktitle = {21st International Conference on Emerging Technologies and Factory Automation (ETFA), 6-9 Sept. 2016, Berlin}, publisher = {IEEE}, address = {Piscataway, NJ}, isbn = {978-1-5090-1314-2}, doi = {10.1109/ETFA.2016.7733673}, pages = {4}, abstract = {In addition to the already required functionality, future production systems should consider the requirements of flexible and demand-oriented resource utilization. Those are not only related on fields of energy, but also material-efficient and time-efficient use of available means of production. Modular system architectures and modular solutions facilitate the planning and implementation of these requirements. Using service-oriented architectures in automation allow an approach for new or modified system components that can be integrated in the available plant environment without a high additional investment of project engineering. This aspect is interesting for companies with manageable machinery, which are able to react immediately and flexible to changes. Service-oriented solutions include among other things the identification, classification and synchronization of possible services. The paper will show general prerequisites for an implementation of a service-oriented architecture in a module-based test field.}, language = {en} } @inproceedings{BergerSchaeferSchoettkeetal., author = {Berger, Ulrich and Sch{\"a}fer, Stephan and Sch{\"o}ttke, Dirk and K{\"a}mpfe, Thomas and Kr{\"o}nke, Dietrich}, title = {Fertigungsanlagen flexibel gestalten - Konzept einer plattform- und serviceorientierten L{\"o}sung}, series = {Entwurf komplexer Automatisierungssysteme, EKA 2016, 24.-25.05.2016, 14. Fachtagung mit Tutorium}, booktitle = {Entwurf komplexer Automatisierungssysteme, EKA 2016, 24.-25.05.2016, 14. Fachtagung mit Tutorium}, publisher = {Institut f{\"u}r Automation und Kommunikation e.V.}, address = {Magdeburg}, isbn = {978-3-944722-35-1}, pages = {1 -- 12}, language = {de} } @misc{BergerSchaeferSchoettkeetal., author = {Berger, Ulrich and Sch{\"a}fer, Stephan and Sch{\"o}ttke, Dirk and K{\"a}mpfe, Thomas and Kr{\"o}nke, Dietrich and Tauber, Bernd}, title = {Flexible Erweiterung von Arbeitsr{\"a}umen}, series = {atp edition}, volume = {58}, journal = {atp edition}, number = {7-8}, issn = {2364-3137}, pages = {58 -- 67}, language = {de} } @misc{KammererJahnWinteretal., author = {Kammerer, Sarah and Jahn, Stephan Wenzel and Winter, Elke and Eidenhammer, Sylvia and Rezania, Simin and Regitnig, Peter and Pichler, Martin and Schreibmayer, Wolfgang and Bauernhofer, Thomas}, title = {Critical evaluation of KCNJ3 gene product detection in human breast cancer: MRNA in situ hybridisation is superior to immunohistochemistry}, series = {Journal of Clinical Pathology}, volume = {69}, journal = {Journal of Clinical Pathology}, number = {12}, issn = {1472-4146}, doi = {10.1136/jclinpath-2016-203798}, pages = {1116 -- 1121}, abstract = {Increased expression levels of KCNJ3 have been correlated with lymph node metastases and poor prognosis in patients with breast cancer, suggesting a prognostic role of KCNJ3. We aimed to establish protocols for the detection of KCNJ3 in formalin-fixed, paraffin-embedded (FFPE) breast cancer tissue. Several antibodies were tested for sensitivity and specificity by western blot, followed by optimisation of the immunohistochemistry (IHC) procedure and establishment of KCNJ3 mRNA in situ hybridisation (ISH). Methods were validated by processing 15 FFPE breast cancer samples for which microarray data were available. Spearman's rank correlation analysis resulted in borderline significant correlation for IHC versus ISH (rS: 0.625; p<0.05) and IHC versus microarray (rS: 0.668; p<0.01), but in significant correlation for ISH versus microarray (rS: 0.861; p<0.001). The ISH method was superior to IHC, regarding robustness, sensitivity and specificity and will aid to further study expression levels of KCNJ3 in both malignant and physiological conditions.}, language = {en} } @misc{KammererSokolowskiHackletal., author = {Kammerer, Sarah and Sokolowski, Armin Andreas and Hackl, Hubert and Platzer, Dieter and Jahn, Stephan Wenzel and El-Heliebi, Amin and Schwarzenbacher, Daniela and Stiegelbauer, Verena and Pichler, Martin and Rezania, Simin and Fiegl, Heidelinde and Peintinger, Florentia and Regitnig, Peter and H{\"o}fler, Gerald and Schreibmayer, Wolfgang and Bauernhofer, Thomas}, title = {KCNJ3 is a new independent prognostic marker for estrogen receptor positive breast cancer patients}, series = {OncoTarget : open access impact journal}, volume = {7}, journal = {OncoTarget : open access impact journal}, number = {51}, doi = {10.18632/oncotarget.13224}, pages = {84705 -- 84717}, abstract = {Numerous studies showed abnormal expression of ion channels in different cancer types. Amongst these, the potassium channel gene KCNJ3 (encoding for GIRK1 proteins) has been reported to be upregulated in tumors of patients with breast cancer and to correlate with positive lymph node status. We aimed to study KCNJ3 levels in different breast cancer subtypes using gene expression data from the TCGA, to validate our findings using RNA in situ hybridization in a validation cohort (GEO ID GSE17705), and to study the prognostic value of KCNJ3using survival analysis. In a total of > 1000 breast cancer patients of two independent data sets we showed a) that KCNJ3 expression is upregulated in tumor tissue compared to corresponding normal tissue (p < 0.001), b) that KCNJ3 expression is associated with estrogen receptor (ER) positive tumors (p < 0.001), but that KCNJ3 expression is variable within this group, and c) that ER positive patients with high KCNJ3 levels have worse overall (p < 0.05) and disease free survival probabilities (p < 0.01), whereby KCNJ3 is an independent prognostic factor (p <0.05). In conclusion, our data suggest that patients with ER positive breast cancer might be stratified into high risk and low risk groups based on the KCNJ3 levels in the tumor.}, language = {en} }