@misc{SchierackHeidenKhanetal., author = {Schierack, Peter and Heiden, Stefan E. and Khan, Muhammad Moman and Nikolaus, Lena and Kolenda, Rafał and Stubbe, Michael and Lkhagvasuren, Davaa and R{\"o}diger, Stefan and Guenther, Sebastian and Schaufler, Katharina}, title = {Genomic and Phenotypic Analysis of an ESBL-Producing E. coli ST1159 Clonal Lineage From Wild Birds in Mongolia}, series = {Frontiers in Microbiology}, volume = {11}, journal = {Frontiers in Microbiology}, issn = {1664-302X}, doi = {10.3389/fmicb.2020.01699}, pages = {7}, language = {en} } @misc{ReimannRoedigerSteinetal., author = {Reimann, Ronny and R{\"o}diger, Stefan and Stein, Erhard and Kilian, Andr{\`e} and Dittrich, Corinne and Petrick, Ingolf}, title = {Die Beschreibung der Fluiddynamik und der Lichtintensit{\"a}t im neuen MULT Reaktor}, publisher = {DECHEMA}, address = {Frankfurt, M.}, pages = {S. 30}, language = {de} } @misc{GeorgeRoedigerSchroederetal., author = {George, Sandra and R{\"o}diger, Stefan and Schr{\"o}der, Christian and Knaut, Michael and K{\"u}pper, Jan-Heiner}, title = {Development of multiplex PCR systems for expression profiling of human cardiomyocytes induced to profilerate by lentivirus transduction of upcyte genes}, series = {Journal of Cellular Biotechnology}, volume = {2}, journal = {Journal of Cellular Biotechnology}, number = {1}, issn = {2352-3697}, doi = {10.3233/JCB-15025}, pages = {35 -- 55}, language = {en} } @misc{GrunerMoncsekRoedigeretal., author = {Gruner, Melanie and Moncsek, Anja and R{\"o}diger, Stefan and K{\"u}hnhardt, Dagmar and Feist, Eugen and Stohwasser, Ralf}, title = {Increased proteasome activator 28 gamma (PA28g) levels are unspecific but correlate with disease activity in rheumatoid arthritis}, series = {BMC musculoskeletal disorders}, volume = {15}, journal = {BMC musculoskeletal disorders}, issn = {1471-2474}, doi = {10.1186/1471-2474-15-414}, pages = {414 -- 424}, language = {en} } @misc{ChoiSchmidtTinnefeldetal., author = {Choi, Youngeun and Schmidt, Carsten and Tinnefeld, Philip and Bald, Ilko and R{\"o}diger, Stefan}, title = {A new reporter design based on DNA origami nanostructures for quantification of short oligonucleotides using microbeads}, series = {Scientific Reports}, journal = {Scientific Reports}, number = {9}, issn = {2045-2322}, doi = {10.1038/s41598-019-41136-x.}, language = {en} } @misc{WhaleSpiegelaereTrypsteenetal., author = {Whale, Alexandra S. and Spiegelaere, Ward De and Trypsteen, W. and Nour, Afif Abdel and Bae, Young-Kyung and Benes, Vladimir and Cleveland, Megan and Devonshire, Alison S. and Dong, Lianhua and Drandi, Daniela and Foy, Carole A. and Garson, Jeremy A. and He, Hua-Jun and Hellemans, Jan and Kubista, Mikael and Lievens, Antoon and Makrigiogos, Mike G. and M{\"u}ller, Reinhold D. and Nolan, Tania and O'Sullivan, Denise M. and Pfaffl, Michael W. and R{\"o}diger, Stefan and Romsos, Erica L. and Shipley, Gregory L. and Taly, Valerie and Untergasser, Andreas and Wittwer, Carl T. and Bustin, Stephen A. and Vandesompele, Jo and Huggett, Jim F.}, title = {The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020}, series = {Clinical Chemistry}, volume = {66}, journal = {Clinical Chemistry}, number = {8}, issn = {0009-9147}, doi = {10.1093/clinchem/hvaa125}, pages = {1012 -- 1029}, language = {en} } @misc{DinterJurischkaWeissetal., author = {Dinter, Franziska and Jurischka, Christoph and Weiss, Romano and R{\"o}diger, Stefan}, title = {Multiparametrische Antik{\"o}rperdetektion f{\"u}r die Diagnostik}, series = {BIOspektrum}, volume = {26}, journal = {BIOspektrum}, number = {3}, issn = {1868-6249}, doi = {10.1077/s12268-020-1375-5}, pages = {265 -- 267}, language = {de} } @misc{ChilimoniukGosiewskaSłowiketal., author = {Chilimoniuk, Jarosław and Gosiewska, Alicja and Słowik, Jadwiga and Weiss, Romano and Deckert, P. Markus and R{\"o}diger, Stefan and Burdukiewicz, Michał}, title = {coutfitteR: efficient selection of count distributions to assess DNA damage}, series = {Annals of Translational Medicine}, volume = {9}, journal = {Annals of Translational Medicine}, number = {7}, issn = {2305-5847}, doi = {10.21037/atm-20-6363}, language = {en} } @misc{FischerMarchettiDeschmannAssisetal., author = {Fischer, Thomas and Marchetti-Deschmann, Martina and Assis, Ana Cristina and Elad, Michal Levin and Algarra, Manuel and Barac, Marko and Bogdanovic Radovic, Iva and Cicconi, Flavio and Claes, Britt and Frascione, Nunzianda and George, Sony and Guedes, Alexandra and Heaton, Cameron and Heeren, Ron and Lasic, Violeta and Lerma, Jos{\´e} Luis and del Valle Martinez de Yuso Garcia, Maria and Nosko, Martin and O'Hara, John and Oshina, Ilze and Palucci, Antonio and Pawlaczyk, Aleksandra and Posp{\´i}škov{\´a}, Krist{\´y}na and de Puit, Marcel and Radodic, Ksenija and Rēpele, Māra and Ristova, Mimoza and Romolo, Francesco Saverio and Šafař{\´i}k, Ivo and Siketic, Zdravko and Spigulis, Janis and Szynkowska-Jozwik, Malgorzata Iwona and Tsiatsiuyeu, Andrei and Vella, Joanna and Dawson, Lorna and R{\"o}diger, Stefan and Francese, Simona}, title = {Profiling and imaging of forensic evidence - A pan-European forensic round robin study part 1: Document forgery}, series = {Science \& Justice}, volume = {62}, journal = {Science \& Justice}, number = {4}, issn = {1876-4452}, doi = {10.1016/j.scijus.2022.06.001}, pages = {433 -- 447}, language = {en} } @inproceedings{RoedigerBurdukiewicz, author = {R{\"o}diger, Stefan and Burdukiewicz, Michał}, title = {Reproducible Analysis of DNA Amplification Experiments with an Open Source Software Environment}, series = {Abstractbook, 67th Annual Meeting of the German Society of Hygiene and Microbiology (DGHM), 27.-30.09.2015, M{\"u}nster}, booktitle = {Abstractbook, 67th Annual Meeting of the German Society of Hygiene and Microbiology (DGHM), 27.-30.09.2015, M{\"u}nster}, language = {en} } @inproceedings{Roediger, author = {R{\"o}diger, Stefan}, title = {Applications of a Multipurpose Platf orm for the Detection and Analysis of Biomolecules}, series = {BioSensor, 9. Deutsches BioSensor Symposium, 11.-13. M{\"a}rz 2015}, booktitle = {BioSensor, 9. Deutsches BioSensor Symposium, 11.-13. M{\"a}rz 2015}, publisher = {M{\"u}nchen}, language = {en} } @misc{SpiessRoedigerBurdukiewiczetal., author = {Spiess, Andrej-Nikolai and R{\"o}diger, Stefan and Burdukiewicz, Michał and Volksdorf, Thomas and Tellinghuisen, Joel}, title = {System-specific periodicity in quantitative real-time polymerase chain reaction data questions threshold-based quantitation}, series = {Scientific Reports}, volume = {6}, journal = {Scientific Reports}, issn = {2045-2322}, doi = {10.1038/srep38951}, pages = {10}, language = {en} } @misc{BurdukiewiczSobczykRoedigeretal., author = {Burdukiewicz, Michał and Sobczyk, Piotr and R{\"o}diger, Stefan and Duda-Madej, Anna and Mackiewicz, Pawel and Kotulska, Malgorzata}, title = {Amyloidogenic motifs revealed by n-gram analysis}, series = {Scientific Reports}, volume = {7}, journal = {Scientific Reports}, issn = {2045-2322}, doi = {10.1038/s41598-017-13210-9}, pages = {12}, language = {en} } @misc{RoedigerLiefoldRuheetal., author = {R{\"o}diger, Stefan and Liefold, Marius and Ruhe, Madeleine and Reinwald, Mark and Beck, Eberhard and Deckert, P. Markus}, title = {Quantification of DNA double-strand breaks in peripheral blood mononuclear cells from healthy donors exposed to bendamustine by an automated H2AX assay- an exploratory study}, series = {Journal of Laboratory and Precision Medicine}, volume = {3}, journal = {Journal of Laboratory and Precision Medicine}, number = {47}, issn = {2519-9005}, doi = {10.21037/jlpm.2018.04.10}, pages = {12}, language = {en} } @misc{SchmidtRoedigerGruneretal., author = {Schmidt, Carsten and R{\"o}diger, Stefan and Gruner, Melanie and Moncsek, Anja and Stohwasser, Ralf and Hanack, Katja and Schierack, Peter and Schr{\"o}der, Christian}, title = {Multiplex localization of sequential peptide epitopes by use of a planar microbead chip}, series = {Analytica Chimica Acta}, volume = {908}, journal = {Analytica Chimica Acta}, issn = {0003-2670}, doi = {10.1016/j.aca.2015.12.030}, pages = {150 -- 160}, language = {en} } @misc{RuheRabeJurischkaetal., author = {Ruhe, Madeleine and Rabe, Dominik and Jurischka, Christoph and Schr{\"o}der, Julia and Schierack, Peter and Deckert, P. Markus and R{\"o}diger, Stefan}, title = {Molecular biomarkers of DNA damage in diffuse large-cell lymphoma-a review}, series = {Journal of Laboratory and Precision Medicine}, volume = {4}, journal = {Journal of Laboratory and Precision Medicine}, issn = {2519-9005}, doi = {10.21037/jlpm.2019.01.01}, pages = {20}, language = {en} } @misc{SchmidtBerghausBlessingetal., author = {Schmidt, Jonas and Berghaus, Sandro and Blessing, Frithjof and Wenzel, Folker and Herbeck, Holger and Blessing, Josef and Schierack, Peter and R{\"o}diger, Stefan and Roggenbuck, Dirk}, title = {A semi-automated, isolation-free, high-throughput SARS-CoV-2 reverse transcriptase (RT) loop-mediated isothermal amplification (LAMP) test}, series = {Scientific reports}, volume = {11}, journal = {Scientific reports}, number = {1}, issn = {2045-2322}, doi = {10.1038/s41598-021-00827-0}, language = {en} } @misc{FroemmelLehmannRoedigeretal., author = {Fr{\"o}mmel, Ulrike and Lehmann, Werner and R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Weinreich, J{\"o}rg and Groß, Julia and Roggenbuck, Dirk and Zinke, Olaf and Ansorge, Hermann and Vogel, Steffen and Klemm, Per and Wex, Thomas and Schr{\"o}der, Christian and Wieler, Lothar H. and Schierack, Peter}, title = {Adhesion of human and animal Escherichia coli strains in association with their virulence-associated genes and phylogenetic origins}, series = {Applied and environmental microbiology}, volume = {79}, journal = {Applied and environmental microbiology}, number = {19}, issn = {1098-5336}, doi = {10.1128/AEM.01384-13}, pages = {5814 -- 5829}, abstract = {Intestinal colonization is influenced by the ability of the bacterium to inhabit a niche, which is based on the expression of colonization factors. Escherichia coli carries a broad range of virulence-associated genes (VAGs) which contribute to intestinal (inVAGs) and extraintestinal (exVAGs) infection. Moreover, initial evidence indicates that inVAGs and exVAGs support intestinal colonization. We developed new screening tools to genotypically and phenotypically characterize E. coli isolates originating in humans, domestic pigs, and 17 wild mammal and avian species. We analyzed 317 isolates for the occurrence of 44 VAGs using a novel multiplex PCR microbead assay (MPMA) and for adhesion to four epithelial cell lines using a new adhesion assay. We correlated data for the definition of new adhesion genes. inVAGs were identified only sporadically, particularly in roe deer (Capreolus capreolus) and the European hedgehog ( Erinaceus europaeus). The prevalence of exVAGs depended on isolation from a specific host. Human uropathogenic E. coli isolates carried exVAGs with the highest prevalence, followed by badger (Meles meles) and roe deer isolates. Adhesion was found to be very diverse. Adhesion was specific to cells, host, and tissue, though it was also unspecific. Occurrence of the following VAGs was associated with a higher rate of adhesion to one or more cell lines: afa-dra, daaD, tsh, vat, ibeA, fyuA, mat, sfa-foc, malX, pic, irp2, and papC. In summary, we established new screening methods which enabled us to characterize large numbers of E. coli isolates. We defined reservoirs for potential pathogenic E. coli. We also identified a very broad range of colonization strategies and defined potential new adhesion genes.}, language = {en} } @misc{SchierackRoedigerKuhletal., author = {Schierack, Peter and R{\"o}diger, Stefan and Kuhl, Christoph and Hiemann, Rico and Roggenbuck, Dirk and Li, Ganwu and Weinreich, J{\"o}rg and Berger, Enrico and Nolan, Lisa K. and Nicholson, Bryon and R{\"o}mer, Antje and Fr{\"o}mmel, Ulrike and Wieler, Lothar H. and Schr{\"o}der, Christian}, title = {Porcine E. coli: Virulence-Associated Genes, Resistance Genes and Adhesion and Probiotic Activity Tested by a New Screening Method}, series = {PLoS One}, volume = {8}, journal = {PLoS One}, number = {4}, doi = {10.1371/journal.pone.0059242}, pages = {13}, abstract = {We established an automated screening method to characterize adhesion of Escherichia coli to intestinal porcine epithelial cells (IPEC-J2) and their probiotic activity against infection by enteropathogenic E. coli (EPEC). 104 intestinal E. coli isolates from domestic pigs were tested by PCR for the occurrence of virulence-associated genes, genes coding for resistances to antimicrobial agents and metals, and for phylogenetic origin by PCR. Adhesion rates and probiotic activity were examined for correlation with the presence of these genes. Finally, data were compared with those from 93 E. coli isolates from wild boars. Isolates from domestic pigs carried a broad variety of all tested genes and showed great diversity in gene patterns. Adhesions varied with a maximum of 18.3 or 24.2 mean bacteria adherence per epithelial cell after 2 or 6 hours respectively. Most isolates from domestic pigs and wild boars showed low adherence, with no correlation between adhesion/probiotic activity and E. coli genes or gene clusters. The gene sfa/foc, encoding for a subunit of F1C fimbriae did show a positive correlative association with adherence and probiotic activity; however E. coli isolates from wild boars with the sfa/foc gene showed less adhesion and probiotic activity than E. coli with the sfa/foc gene isolated from domestic pigs after 6 hour incubation. In conclusion, screening porcine E. coli for virulence associated genes genes, adhesion to intestinal epithelial cells, and probiotic activity revealed a single important adhesion factor, several probiotic candidates, and showed important differences between E. coli of domestic pigs and wild boars.}, language = {en} } @misc{RoedigerLehmannFroemmeletal., author = {R{\"o}diger, Stefan and Lehmann, Werner and Fr{\"o}mmel, Ulrike and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Burdukiewicz, Michał and Schr{\"o}der, Christian and Dangla, R{\´e}mi and Droniou, Magali and Schierack, Peter}, title = {The Modi Operandi of the VideoScan Platform for the Detection and Analysis of Nucleic Acids. Life Science Day 2013 \& 17. Leibniz Conference of Advanced Science, Freie Universit{\"a}t Berlin, 24.10.2013}, pages = {1}, language = {en} }