@misc{CarlssonHerzogFelskeetal., author = {Carlsson, Max J. and Herzog, Natalie and Felske, Christina and Ackermann, Gabriel and Regier, Alexander and Wittmann, Simon and Fern{\´a}ndez Cereijo, Ra{\´u}l and Sturla, Shana J. and K{\"u}pper, Jan-Heiner and Fahrer, J{\"o}rg}, title = {The DNA repair protein MGMT protects against the genotoxicity of N-nitrosodimethylamine, but Not N-nitrosodiethanolamine and N-nitrosomethylaniline, in human HepG2 liver cells with CYP2E1 expression}, series = {Chemical research in toxicology}, volume = {38}, journal = {Chemical research in toxicology}, number = {6}, publisher = {American Chemical Society (ACS)}, address = {New York, NY}, issn = {0893-228X}, doi = {10.1021/acs.chemrestox.5c00133}, pages = {1134 -- 1146}, abstract = {N-nitrosamines are genotoxic contaminants that occur in the diet, consumer products, and the environment. More recently, N-nitrosamines were also detected as drug impurities. After uptake, N-nitrosamines undergo metabolic activation by cytochrome P450 monooxygenases (CYPs), resulting in DNA damage and tumor formation. In this study, the genotoxicity and cytotoxicity of three N-nitrosamines with structurally distinct substituents, N-nitrosodimethylamine (NDMA), N-nitrosodiethanolamine (NDELA) and N-nitrosomethylaniline (NMA), were analyzed in human HepG2 liver cell models proficient or deficient in CYP2E1 biotransformation. Furthermore, the impact of the DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT) was investigated. The novel genetically engineered HepG2-CYP2E1 cell line strongly expressed CYP2E1, which was not detectable in wildtype (WT) HepG2 cells. We then confirmed that the CYP2E1 substrate NDMA caused O6-methyldesoxyguanosine adducts and DNA strand breaks in a CYP2E1-dependent manner, leading to cytotoxicity. By the same approach, we demonstrated that NDELA induced DNA strand breaks in HepG2-CYP2E1 cells, whereas no effect was observed for NMA. However, NMA was revealed to cause DNA cross-links. Furthermore, both NDELA and NMA were cytotoxic in HepG2-CYP2E1 cells, but not in WT cells. Subsequently, the pharmacological MGMT inhibitor O6-benzylguanine was used to deplete MGMT in both HepG2 cell models. MGMT inhibition clearly increased DNA strand break levels due to NDMA exposure, whereas DNA strand break formation by NDELA and NMA were not affected by inhibiting MGMT. In line with these findings, the clastogenic effects of NDMA were potentiated in the absence of MGMT. In contrast to that, NDELA- and NMA-induced clastogenicity was not influenced by MGMT inhibition. Taken together, our study revealed that all three structurally diverse N-nitrosamines are cytotoxic and clastogenic in a CYP2E1-dependent manner, while only NDMA and NDELA caused DNA strand breaks. Furthermore, we demonstrated for the first time that DNA repair by MGMT does not confer protection against NDELA and NMA-triggered DNA strand break induction and clastogenicity.}, language = {en} } @misc{MartienssenSimonSchulze, author = {Martienssen, Marion and Simon, J. and Schulze, R.}, title = {Untersuchungen zum Einsatz von aufbereiteter Kfz-K{\"u}hlerfl{\"u}ssigkeit als organische C-Quelle bei der biologischen Sickerwasserbehandlung}, series = {Korrespondenz Abwasser}, volume = {44}, journal = {Korrespondenz Abwasser}, number = {9}, issn = {0341-1540}, pages = {1622 -- 1627}, language = {de} } @misc{MartienssenSimon, author = {Martienssen, Marion and Simon, J.}, title = {Reinigung von Abwasser aus Emulsionsspaltanlagen durch kombinierten Einsatz von biologischer Behandlung und chemischer Naßoxidation}, series = {Korespondenz Abwasser}, volume = {44}, journal = {Korespondenz Abwasser}, number = {1}, issn = {0341-1540}, pages = {110 -- 114}, language = {de} } @inproceedings{MartienssenSchulzeHeinkeetal., author = {Martienssen, Marion and Schulze, R. and Heinke, M. and Pohl, W. and Simon, J.}, title = {Leistungen und Leistungsgrenzen unterschiedlicher biotechnologischer Verfahren f{\"u}r die Behandlung von Deponiesickerwasser}, series = {11. Jahrestagung der Biotechnologen, N{\"u}rnberg, 24. bis 26. Mai 1993, Kurzfassungen}, booktitle = {11. Jahrestagung der Biotechnologen, N{\"u}rnberg, 24. bis 26. Mai 1993, Kurzfassungen}, publisher = {DECHEMA}, address = {Frankfurt am Main}, pages = {43 -- 44}, language = {de} } @misc{MartienssenSchulzeSimon, author = {Martienssen, Marion and Schulze, R. and Simon, J.}, title = {Capacities and limits of three different technologies for the biological treatment of leachate from solid waste landfill sites}, language = {en} } @misc{MartienssenSimon, author = {Martienssen, Marion and Simon, J.}, title = {Effect of activated carbon on the biological treatment of oil-water emulsions}, language = {en} } @misc{MuellerTorgerAllertzetal., author = {M{\"u}ller, Felix and Torger, Bernhard and Allertz, Peter J. and J{\"a}hnichen, Klaus and Keßler, Stefan and M{\"u}ller, Martin and Simon, Frank and Salchert, Katrin and M{\"a}urer, Haike and Pospiech, Doris}, title = {Multifunctional crosslinkable itaconic acid copolymers for enzyme immobilization}, series = {European Polymer Journal}, volume = {102}, journal = {European Polymer Journal}, doi = {10.1016/j.eurpolymj.2018.03.014}, pages = {47 -- 55}, abstract = {UV-Crosslinkable itaconic copolymers are developed to provide new multifunctional materials for coatings which combine crosslinkable functionalities and the possibility to immobilize enzymes. The polymer-immobilized enzymes were used for water treatment to decompose persistent organic molecules. Introduction of suitable comonomers allows tailoring the mechanical and chemical properties for special applications. Copolymers containing MMA and itaconic anhydride were chosen because of the formation of long-term stable anhydride functionalities. These anhydride functionalities are employed to attach enzymes covalently. 4-Benzoylphenyl methacrylate is used as comonomer for UV-initiated crosslinking. Terpolymers are successfully obtained by radical copolymerization in solution. The copolymers are compared to poly(ethylene-alt-maleic anhydride) [P(EMA)] often used with respect to enzyme immobilization, activity and hydrolytic stability. The hydrolysis stability of the copolymers against water is studied by ATR-FTIR spectroscopy. Thin films are prepared on glass substrates in a layer-by-layer procedure by spin-coating. The layer formation is monitored by ATR-FTIR spectroscopy. UV-crosslinking of the copolymer films is performed taking the optimal irradiation dose that avoids polymer degradation. ATR-FTIR spectroscopy verifies the coupling reaction between amino groups of the enzyme and the anhydride groups on the surface of the crosslinked polymer film. The syringaldazine (4-hydroxy-3,5-dimethoxybenzaldehyde azine) test and 2,2´-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assay demonstrate that the immobilized enzymes maintain their activities. The functional copolymers showed a significant effect in reduction of persistent organic pollutants in contaminated waste water.}, language = {de} } @misc{KrisponeitFischerEsseretal., author = {Krisponeit, Jon-Olaf and Fischer, Simon and Esser, Sven and Moshnyaga, Vasily and Schmidt, Thomas and Piper, Louis F. J. and Flege, Jan Ingo and Falta, Jens}, title = {The morphology of VO2/TiO2(001): terraces, facets, and cracks}, series = {Scientific Reports}, volume = {10}, journal = {Scientific Reports}, issn = {2045-2322}, doi = {10.1038/s41598-020-78584-9}, pages = {8}, abstract = {Vanadium dioxide (VO2) features a pronounced, thermally-driven metal-to-insulator transition at 340 K. Employing epitaxial stress on rutile TiO2(001) substrates, the transition can be tuned to occur close to room temperature. Striving for applications in oxide-electronic devices, the lateral homogeneity of such samples must be considered as an important prerequisite for efforts towards miniaturization. Moreover, the preparation of smooth surfaces is crucial for vertically stacked devices and, hence, the design of functional interfaces. Here, the surface morphology of VO2/TiO2(001) films was analyzed by low-energy electron microscopy and diffraction as well as scanning probe microscopy. The formation of large terraces could be achieved under temperature-induced annealing, but also the occurrence of facets was observed and characterized. Further, we report on quasi-periodic arrangements of crack defects which evolve due to thermal stress under cooling. While these might impair some applicational endeavours, they may also present crystallographically well-oriented nano-templates of bulk-like properties for advanced approaches.}, language = {en} } @misc{LyuFuWilczoketal., author = {Lyu, Yu-Xuan and Fu, Qiang and Wilczok, Dominika and Ying, Kejun and King, Aaron and Antebi, Adam and Vojta, Aleksandar and Stolzing, Alexandra and Moskalev, Alexey and Georgievskaya, Anastasia and Maier, Andrea B. and Olsen, Andrea and Groth, Anja and Simon, Anna Katharina and Brunet, Anne and Jamil, Aisyah and Kulaga, Anton and Bhatti, Asif and Yaden, Benjamin and Pedersen, Bente Klarlund and Schumacher, Bj{\"o}rn and Djordjevic, Boris and Kennedy, Brian and Chen, Chieh and Huang, Christine Yuan and Correll, Christoph U. and Murphy, Coleen T. and Ewald, Collin Y. and Chen, Danica and Valenzano, Dario Riccardo and Sołdacki, Dariusz and Erritzoe, David and Meyer, David and Sinclair, David A. and Chini, Eduardo Nunes and Teeling, Emma C. and Morgen, Eric and Verdin, Eric and Vernet, Erik and Pinilla, Estefano and Fang, Evandro F. and Bischof, Evelyne and Mercken, Evi M. and Finger, Fabian and Kuipers, Folkert and Pun, Frank W. and Gy{\"u}lveszi, Gabor and Civiletto, Gabriele and Zmudze, Garri and Blander, Gil and Pincus, Harold A. and McClure, Joshua and Kirkland, James L. and Peyer, James and Justice, Jamie N. and Vijg, Jan and Gruhn, Jennifer R. and McLaughlin, Jerry and Mannick, Joan and Passos, Jo{\~a}o and Baur, Joseph A. and Betts-LaCroix, Joe and Sedivy, John M. and Speakman, John R. and Shlain, Jordan and Maltzahn, Julia von and Andreasson, Katrin I. and Moody, Kelsey and Palikaras, Konstantinos and Fortney, Kristen and Niedernhofer, Laura J. and Rasmussen, Lene Juel and Veenhoff, Liesbeth M. and Melton, Lisa and Ferrucci, Luigi and Quarta, Marco and Koval, Maria and Marinova, Maria and Hamalainen, Mark and Unfried, Maximilian and Ringel, Michael S. and Filipovic, Milos and Topors, Mourad and Mitin, Natalia and Roy, Nawal and Pintar, Nika and Barzilai, Nir and Binetti, Paolo and Singh, Parminder and Kohlhaas, Paul and Robbins, Paul D. and Rubin, Paul and Fedichev, Peter O. and Kamya, Petrina and Mu{\~n}oz-Canoves, Pura and de Cabo, Rafael and Faragher, Richard G. A. and Konrad, Rob and Ripa, Roberto and Mansukhani, Robin and B{\"u}ttner, Sabrina and Wickstr{\"o}m, Sara A. and Brunemeier, Sebastian and Jakimov, Sergey and Luo, Shan and Rosenzweig-Lipson, Sharon and Tsai, Shih-Yin and Dimmeler, Stefanie and Rando, Thomas A. and Peterson, Tim R. and Woods, Tina and Wyss-Coray, Tony and Finkel, Toren and Strauss, Tzipora and Gladyshev, Vadim N. and Longo, Valter D. and Dwaraka, Varun B. and Gorbunova, Vera and Acosta-Rodr{\´i}guez, Victoria A. and Sorrentino, Vincenzo and Sebastiano, Vittorio and Li, Wenbin and Suh, Yousin and Zhavoronkov, Alex and Scheibye-Knudsen, Morten and Bakula, Daniela}, title = {Longevity biotechnology: bridging AI, biomarkers, geroscience and clinical applications for healthy longevity}, series = {Aging}, volume = {16}, journal = {Aging}, number = {20}, publisher = {Impact Journals, LLC}, issn = {1945-4589}, doi = {10.18632/aging.206135}, pages = {12955 -- 12976}, language = {en} }