@misc{RoggenbuckGoihlHanacketal., author = {Roggenbuck, Dirk and Goihl, Alexander and Hanack, Katja and Holzl{\"o}hner, Pamela and Hentschel, Christian and Veiczi, Miklos and Schierack, Peter and Reinhold, Dirk and Schulz, Hans-Ulrich}, title = {Serological diagnosis and prognosis of severe acute pancreatitis by analysis of serum glycoprotein 2}, series = {Clinical Chemistry and Laboratory Medicine}, volume = {55}, journal = {Clinical Chemistry and Laboratory Medicine}, number = {6}, issn = {1437-4331}, doi = {10.1515/cclm-2016-0797}, pages = {854 -- 864}, language = {en} } @misc{JurischkaDinterEfimovaetal., author = {Jurischka, Christoph and Dinter, Franziska and Efimova, Anastasia and Weiss, Romano and Schiebel, Juliane and Schulz, Christian and Fayziev, Bekzodjon and Schierack, Peter and Fischer, Thomas and R{\"o}diger, Stefan}, title = {An explorative study of polymers for 3D printing of bioanalytical test systems}, series = {Clinical Hemorheology and Microcirculation}, volume = {75}, journal = {Clinical Hemorheology and Microcirculation}, number = {1}, issn = {1875-8622}, doi = {10.3233/CH-190713}, pages = {57 -- 84}, abstract = {Background: The 3D printing is relevant as a manufacturing technology of functional models for forensic, pharmaceutical and bioanalytical applications such as drug delivery systems, sample preparation and point-of-care tests. Objective: Melting behavior and autofluorescence of materials are decisive for optimal printing and applicability of the product which are influenced by varying unknown additives. Methods: We have produced devices for bioanalytical applications from commercially available thermoplastic polymers using a melt-layer process. We characterized them by differential scanning calorimetry, fluorescence spectroscopy and functional assays (DNA capture assay, model for cell adhesion, bacterial adhesion and biofilm formation test). Results: From 14 tested colored, transparent and black materials we found only deep black acrylonitrile-butadiene-styrene (ABS) and some black polylactic acid (PLA) useable for fluorescence-based assays, with low autofluorescence only in the short-wave range of 300-400 nm. PLA was suitable for standard bioanalytical purposes due to a glass transition temperature of approximately 60°C, resistance to common laboratory chemicals and easy print processing. For temperature-critical methods, such as hybridization reactions up to 90°C, ABS was better suited. Conclusions: Autofluorescence was not a disadvantage per se but can also be used as a reference signal in assays. The rapid development of individual protocols for sample processing and analysis required the availability of a material with consistent quality over time. For fluorescence-based assays, the use of commercial standard materials did not seem to meet this requirement.}, language = {en} } @incollection{ElmerHohbergRusselletal., author = {Elmer, Michael and Hohberg, Karin and Russell, D. and Christian, Axel and Schulz, H.- J. and Wanner, Manfred}, title = {Succession of the soil faunal community during initial ecosystem development}, series = {Initial development of the artificial catchment "Chicken Creek" : monitoring program and survey 2005-2008}, booktitle = {Initial development of the artificial catchment "Chicken Creek" : monitoring program and survey 2005-2008}, editor = {Schaaf, Wolfgang and Biemelt, Detlef and H{\"u}ttl, Reinhard F.}, publisher = {Univ. of Technology, Research Center Landscape Development and Mining Landscapes}, address = {Cottbus}, pages = {97 -- 118}, language = {en} } @misc{MahmoodScharobaSchorlemeretal., author = {Mahmood, Safdar and Scharoba, Stefan and Schorlemer, Jonas and Schulz, Christian and H{\"u}bner, Michael and Reichenbach, Marc}, title = {Detecting Improvised Land-mines using Deep Neural Networks on GPR Image Dataset targeting FPGAs }, series = {IEEE Nordic Circuits and Systems Conference (NORCAS), 25-26 October 2022, Oslo, Norway}, journal = {IEEE Nordic Circuits and Systems Conference (NORCAS), 25-26 October 2022, Oslo, Norway}, publisher = {IEEE}, address = {Piscataway, NJ}, isbn = {979-8-3503-4550-6}, doi = {10.1109/norcas57515.2022.9934735}, pages = {1 -- 7}, language = {en} } @misc{KnauerSchulzZemellaetal., author = {Knauer, Jan Felix and Schulz, Christian and Zemella, Anne and W{\"u}stenhagen, Doreen A. and Walter, Ruben Magnus and K{\"u}pper, Jan-Heiner and Kubick, Stefan}, title = {Synthesis of mono Cytochrome P450 in a modified CHO-CPR cell-free protein production platform}, series = {Scientific Reports}, volume = {14}, journal = {Scientific Reports}, issn = {2045-2322}, doi = {10.1038/s41598-024-51781-6}, pages = {14}, abstract = {AbstractCytochromes P450 (CYPs) are a group of monooxygenases that can be found in almost all kinds of organisms. For CYPs to receive electrons from co-substrate NADPH, the activity of NADPH-Cytochrome-P450-oxidoreductase (CPR) is required as well. In humans, CYPs are an integral part of liver-based phase-1 biotransformation, which is essential for the metabolization of multiple xenobiotics and drugs. Consequently, CYPs are important players during drug development and therefore these enzymes are implemented in diverse screening applications. For these applications it is usually advantageous to use mono CYP microsomes containing only the CYP of interest. The generation of mono-CYP containing mammalian cells and vesicles is difficult since endogenous CYPs are present in many cell types that contain the necessary co-factors. By obtaining translationally active lysates from a modified CHO-CPR cell line, it is now possible to generate mono CYPs in a cell-free protein synthesis process in a straightforward manner. As a proof of principle, the synthesis of active human CYPs from three different CYP450 gene families (CYP1A2, CYP2B6 and CYP3A4), which are of outstanding interest in industry and academia was demonstrated. Luciferase based activity assays confirm the activity of the produced CYPs and enable the individual adaptation of the synthesis process for efficient cell-free enzyme production. Furthermore, they allow for substrate and inhibitor screenings not only for wild-type CYPs but also for mutants and further CYP isoforms and variants. As an example, the turnover of selected CYP substrates by cell-free synthesized CYPs was demonstrated via an indirect luciferase assay-based screening setup.}, language = {en} }