@misc{FroemmelLehmannRoedigeretal., author = {Fr{\"o}mmel, Ulrike and Lehmann, Werner and R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Weinreich, J{\"o}rg and Groß, Julia and Roggenbuck, Dirk and Zinke, Olaf and Ansorge, Hermann and Vogel, Steffen and Klemm, Per and Wex, Thomas and Schr{\"o}der, Christian and Wieler, Lothar H. and Schierack, Peter}, title = {Adhesion of human and animal Escherichia coli strains in association with their virulence-associated genes and phylogenetic origins}, series = {Applied and environmental microbiology}, volume = {79}, journal = {Applied and environmental microbiology}, number = {19}, issn = {1098-5336}, doi = {10.1128/AEM.01384-13}, pages = {5814 -- 5829}, abstract = {Intestinal colonization is influenced by the ability of the bacterium to inhabit a niche, which is based on the expression of colonization factors. Escherichia coli carries a broad range of virulence-associated genes (VAGs) which contribute to intestinal (inVAGs) and extraintestinal (exVAGs) infection. Moreover, initial evidence indicates that inVAGs and exVAGs support intestinal colonization. We developed new screening tools to genotypically and phenotypically characterize E. coli isolates originating in humans, domestic pigs, and 17 wild mammal and avian species. We analyzed 317 isolates for the occurrence of 44 VAGs using a novel multiplex PCR microbead assay (MPMA) and for adhesion to four epithelial cell lines using a new adhesion assay. We correlated data for the definition of new adhesion genes. inVAGs were identified only sporadically, particularly in roe deer (Capreolus capreolus) and the European hedgehog ( Erinaceus europaeus). The prevalence of exVAGs depended on isolation from a specific host. Human uropathogenic E. coli isolates carried exVAGs with the highest prevalence, followed by badger (Meles meles) and roe deer isolates. Adhesion was found to be very diverse. Adhesion was specific to cells, host, and tissue, though it was also unspecific. Occurrence of the following VAGs was associated with a higher rate of adhesion to one or more cell lines: afa-dra, daaD, tsh, vat, ibeA, fyuA, mat, sfa-foc, malX, pic, irp2, and papC. In summary, we established new screening methods which enabled us to characterize large numbers of E. coli isolates. We defined reservoirs for potential pathogenic E. coli. We also identified a very broad range of colonization strategies and defined potential new adhesion genes.}, language = {en} } @misc{FroemmelBoehmNitschkeetal., author = {Fr{\"o}mmel, Ulrike and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Weinreich, J{\"o}rg and Groß, Julia and R{\"o}diger, Stefan and Wex, Thomas and Ansorge, Hermann and Zinke, Olaf and Schr{\"o}der, Christian and Roggenbuck, Dirk and Schierack, Peter}, title = {Adhesion patterns of commensal and pathogenic Escherichia coli from humans and wild animals on human and porcine epithelial cell lines}, series = {Gut Pathogens}, volume = {5}, journal = {Gut Pathogens}, number = {31}, issn = {1757-4749}, doi = {10.1186/1757-4749-5-31}, pages = {8}, abstract = {Abstract BACKGROUND: Different strategies of colonization or infection by E. coli result in formation of certain adhesion patterns which help also in classifying intestinal E. coli into pathotypes. Little is known about adhesion patterns and host- and tissue adaption of commensal E. coli and about E. coli originating in clinically healthy hosts carrying pathotype-specific virulence-associated genes. FINDINGS: Adhesion pattern of E. coli (n = 282) from humans and from 18 animal species were verified on intestinal human Caco-2 and porcine IPEC-J2 cells and, furthermore, for comparison on human urinary bladder 5637, porcine kidney PK-15 epithelial and HEp-2 cells. The analysis was carried out on 150,000 images of adhesion assays.Adhesion patterns were very diverse; 88 isolates were completely non-adherent, whereas 194 adhered to at least one cell line with the dominant adhesion patterns "diffusely distributed" and "microcolony formation". Adhesion patterns "chains" and "clumps" were also visible. Chain formation was mediated by the presence of epithelial cells. Clump formation was very specific on only the 5637 cell line. All enteropathogenic (eae+) E. coli (EPEC; n = 14) were able to form microcolonies which was cell line specific for each isolate. Most EPEC formed microcolonies on intestinal IPEC-J2 and Caco-2 but several also on urinary tract cells. Shigatoxin-producing (stx+) E. coli (n = 10) showed no specific adhesion patterns. CONCLUSIONS: E. coli isolates were highly diverse. Commensal and pathogenic isolates can adhere in various forms, including diffuse distribution, microcolonies, chains and clumps. Microcolony formation seems to be a global adhesion strategy also for commensal E. coli.}, language = {en} } @misc{RoedigerRuhlandSchroederetal., author = {R{\"o}diger, Stefan and Ruhland, Mirko and Schr{\"o}der, Christian and Großmann, Kai and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Berger, Ingo and Schimke, Ingolf and Schierack, Peter}, title = {Fluorescence dye adsorption assay to quantify carboxyl groups on the surface of poly(methyl methacrylate) microbeads}, series = {Analytical Chemistry}, volume = {83}, journal = {Analytical Chemistry}, number = {9}, issn = {0003-2700}, doi = {10.1021/ac103277s}, pages = {3379 -- 3385}, language = {en} } @incollection{RoedigerSchierackBoehmetal., author = {R{\"o}diger, Stefan and Schierack, Peter and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Berger, Ingo and Fr{\"o}mmel, Ulrike and Schmidt, Carsten and Ruhland, Mirko and Schimke, Ingolf and Roggenbuck, Dirk and Lehmann, Werner and Schr{\"o}der, Christian}, title = {A highly versatile microscope imaging technology platform for the multiplex real-time detection of biomolecules and autoimmune antibodies}, series = {Molecular diagnostics}, booktitle = {Molecular diagnostics}, editor = {Seitz, Harald and Berger, Ingo}, publisher = {Springer}, address = {Berlin [u.a.]}, isbn = {978-3-642-37690-0}, pages = {35 -- 74}, language = {en} } @misc{SchmidtRoedigerGruneretal., author = {Schmidt, Carsten and R{\"o}diger, Stefan and Gruner, Melanie and Moncsek, Anja and Stohwasser, Ralf and Hanack, Katja and Schierack, Peter and Schr{\"o}der, Christian}, title = {Multiplex localization of sequential peptide epitopes by use of a planar microbead chip}, series = {Analytica Chimica Acta}, volume = {908}, journal = {Analytica Chimica Acta}, issn = {0003-2670}, doi = {10.1016/j.aca.2015.12.030}, pages = {150 -- 160}, language = {en} } @misc{RoedigerKramerFroemmeletal., author = {R{\"o}diger, Stefan and Kramer, Toni and Fr{\"o}mmel, Ulrike and Weinreich, J{\"o}rg and Roggenbuck, Dirk and Guenther, Sebastian and Schaufler, Katharina and Schr{\"o}der, Christian and Schierack, Peter}, title = {Intestinal Escherichia coli colonization in a mallard duck population over four consecutive winter season}, series = {Environmental Microbiology}, volume = {17}, journal = {Environmental Microbiology}, number = {9}, issn = {1462-2920}, doi = {10.1111/1462-2920.12807}, pages = {3352 -- 3361}, language = {en} } @inproceedings{RoedigerBoehmNitschkeetal., author = {R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Stavitskaya, Luba and Gruner, Melanie and Kundzer, Alena V. and Volkova, Margarita V. and Generalov, I. and Schmidt, Carsten and Schr{\"o}der, Christian and Roggenbuck, Dirk and Schierack, Peter}, title = {Development of a Method for Multiplex Real-Time Analysis of Enzymatic Activity on a Microbead-Chip}, series = {Infection, tumors and autoimmunity, report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 1-4, 2013}, booktitle = {Infection, tumors and autoimmunity, report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 1-4, 2013}, publisher = {Pabst Science Publ.}, address = {Lengerich [u.a.]}, isbn = {978-3-89967-881-9}, pages = {275 -- 280}, language = {en} } @misc{RoedigerLehmannFroemmeletal., author = {R{\"o}diger, Stefan and Lehmann, Werner and Fr{\"o}mmel, Ulrike and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Burdukiewicz, Michał and Schr{\"o}der, Christian and Dangla, R{\´e}mi and Droniou, Magali and Schierack, Peter}, title = {The Modi Operandi of the VideoScan Platform for the Detection and Analysis of Nucleic Acids. Life Science Day 2013 \& 17. Leibniz Conference of Advanced Science, Freie Universit{\"a}t Berlin, 24.10.2013}, pages = {1}, language = {en} } @misc{RoedigerSchneiderSaueretal., author = {R{\"o}diger, Stefan and Schneider, Jens and Sauer, Lysann and Reddig, Annika and Schierack, Peter and Roggenbuck, Dirk and Schr{\"o}der, Christian}, title = {A Multiparameter Platform for The Analysis of DNA Double-strand Breaks and Associated Biomarkers after genome Editing Austrian Biomarker Symposium (ASBD) 2016, 10.-11.03.2016, Wien, {\"O}sterreich}, language = {en} } @misc{SauerRoedigerSchneideretal., author = {Sauer, Lysann and R{\"o}diger, Stefan and Schneider, Jens and Schierack, Peter and Roggenbuck, Dirk and Schr{\"o}der, Christian}, title = {Functional Multiparameter Analy sis of Double-Strand Breaks and Associate Biomarkers during Genome Editing, Genome Editing and Gene Modulation Congress 2016, 6.-8.04.2016, Oxford, United Kingdom}, language = {en} } @inproceedings{RoedigerBoehmNitschkeetal., author = {R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Stavitskaya, Luba and Gruner, Melanie and Kundzer, Alena V. and Volkova, Margarita V. and Generalov, I. and Schmidt, Carsten and Schr{\"o}der, Christian and Roggenbuck, Dirk and Schierack, Peter}, title = {Development of a Method for Multiplex Real-Time Analysis of Enzymati c Activity on a Microbead-Chip.}, series = {Infections, Tumors and Autoimmunity, Report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 01. - 04., 2013}, booktitle = {Infections, Tumors and Autoimmunity, Report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 01. - 04., 2013}, publisher = {Pabst Science Publ.}, address = {Lengerich}, isbn = {978-3-89967-881-9}, pages = {280}, language = {en} } @inproceedings{RoedigerHanschmannSteidleetal., author = {R{\"o}diger, Stefan and Hanschmann, Henning and Steidle, Michael and Schr{\"o}der, Christian and Schierack, Peter and Lehmann, Werner}, title = {Detection of Borrelia, Bordetella and HPV with the LoopTag Real-Time PCR Probe System}, series = {International journal of medical microbiology}, volume = {303}, booktitle = {International journal of medical microbiology}, number = {Supplement 1}, issn = {1618-0607}, pages = {12 -- 13}, language = {en} } @techreport{FeindtWoltersBackesetal., author = {Feindt, Peter H. and Wolters, Volkmar and Backes, Gunter and Bahrs, Enno and Brandt, Horst and Engels, Eve-Marie and Engels, Johannes and Graner, Andreas and Hamm, Ulrich and Herdegen, Matthias and Isselstein, Johannes and Schr{\"o}der, Stefan and Tholen, Ernst and Wagner, Sven and W{\"a}tzold, Frank and Wolf, Heino}, title = {Schutz tiergenetischer Ressourcen im Tierseuchenfall - Vorschl{\"a}ge zum Schutz von Tierbest{\"a}nden in Nukleus- bzw. Basiszuchtbetrieben und Besamungsstationen bei Auftreten der Afrikanischen Schweinepest}, publisher = {Bundesanstalt f{\"u}r Landwirtschaft und Ern{\"a}hrung (BLE), Informations- und Koordinationszentrum f{\"u}r Biologische Vielfalt (IBV)}, address = {Bonn}, pages = {9}, language = {de} } @misc{RuheRabeJurischkaetal., author = {Ruhe, Madeleine and Rabe, Dominik and Jurischka, Christoph and Schr{\"o}der, Julia and Schierack, Peter and Deckert, P. Markus and R{\"o}diger, Stefan}, title = {Molecular biomarkers of DNA damage in diffuse large-cell lymphoma-a review}, series = {Journal of Laboratory and Precision Medicine}, volume = {4}, journal = {Journal of Laboratory and Precision Medicine}, issn = {2519-9005}, doi = {10.21037/jlpm.2019.01.01}, pages = {20}, language = {en} } @misc{LehnenSchroederHenningsenetal., author = {Lehnen, Nadine and Schr{\"o}der, Lena and Henningsen, Peter and Glasauer, Stefan and Ramaioli, Cecilia}, title = {Deficient head motor control in functional dizziness: Experimental evidence of central sensory-motor dysfunction in persistent physical symptoms}, series = {Progress in Brain Research}, volume = {249}, journal = {Progress in Brain Research}, issn = {1875-7855}, doi = {10.1016/bs.pbr.2019.02.006}, pages = {385 -- 400}, abstract = {Understanding the mechanisms of symptoms that are insufficiently explained by organic dysfunction remains challenging. Recently, it has been proposed that such "functional symptoms" are based on erroneous sensory processing in the central nervous system (CNS), with internal expectations dominating sensory inputs. In a pilot study, we used a head motor control set-up to assess the interplay between sensory input and expectation on the example of patients with functional dizziness. Eight patients and 11 age-matched healthy controls performed large active eye-head gaze shifts towards visual targets in the natural situation and with the head moment of inertia 3.3-fold increased. The latter induces head oscillations and the expected sensory outcome of the movement, estimated in the CNS, does not match the actual sensory input. Head oscillations were assessed in patients and in healthy subjects and compared to prior results from patients with organic disease (vestibular loss and cerebellar ataxia). Head oscillations in patients with functional dizziness were different from those of healthy subjects (F(1,17) = 27.26, P < 0.001, partial η2 = 0.62), and similar to those of patients with cerebellar ataxia, and with vestibular loss (F(2,19) = 0.56, P = 0.58). Even in the natural, unweighted, condition, head oscillations were higher in functional dizziness patients than in healthy subjects (P = 0.001). Since an extensive work-up failed to demonstrate any explanatory peripheral vestibular, motor, or cerebellar organic dysfunction, these motor control deficits are a first indication of erroneous interplay between expectations and sensory input in the CNS that could account for persistent physical symptoms.}, language = {en} }