@techreport{ZundelKrahlSchroederetal., author = {Zundel, Stefan and Krahl, Susanne and Schr{\"o}der, Wolfgang and Schuster, Helmut}, title = {Oberspreewald Lausitz Strukturentwicklungskonzept : Endbericht}, publisher = {Eigenverlag}, address = {Senftenberg}, pages = {87}, language = {de} } @techreport{FeindtWoltersBackesetal., author = {Feindt, Peter H. and Wolters, Volkmar and Backes, Gunter and Bahrs, Enno and Dempfle, Leo and Engels, Eve-Marie and Engels, Johannes and Graner, Andreas and Hamm, Ulrich and Herdegen, Matthias and Isselstein, Johannes and Konnert, Monika and Schr{\"o}der, Stefan and Schulte-Coerne, Hermann and Wagner, Sven and W{\"a}tzold, Frank and Wedekind, Helmut}, title = {Gr{\"u}ndung der Deutschen Genbank landwirtschaftlicher Nutztiere : Kurzstellungnahme des Wissenschaftlichen Beirats f{\"u}r Biodiversit{\"a}t und Genetische Ressourcen beim Bundesministerium f{\"u}r Ern{\"a}hrung und Landwirtschaft}, publisher = {BMEL}, address = {Bonn}, pages = {10}, language = {de} } @techreport{WaetzoldBahrsFeindtetal., author = {W{\"a}tzold, Frank and Bahrs, Enno and Feindt, Peter H. and Hamm, Ulrich and Isselstein, Johannes and Schr{\"o}der, Stefan and Wolters, Volkmar and Backes, Gunter and Dempfle, Leo and Engels, Eve-Marie and Engels, Johannes and Graner, Andreas and Herdegen, Matthias and Konnert, Monika and Schulte-Coerne, Hermann and Wagner, Sven and Wedekind, Helmut}, title = {Perspektiven f{\"u}r das artenreiche Gr{\"u}nland - Alternativen zur Belohnung einer {\"U}berschussproduktion bei Milch}, address = {Bonn}, pages = {9}, language = {de} } @misc{NuernbergerDrescherRottaetal., author = {N{\"u}rnberger, Stefan and Drescher, Gabor and Rotta, Randolf and Nolte, J{\"o}rg and Schr{\"o}der-Preikschat, Wolfgang}, title = {Shared Memory in the Many-Core Age}, series = {Euro-Par 2014: Parallel Processing Workshops, Porto, 2014}, journal = {Euro-Par 2014: Parallel Processing Workshops, Porto, 2014}, publisher = {Springer International Publishing}, address = {Cham}, isbn = {978-3-319-14312-5}, doi = {10.1007/978-3-319-14313-2_30}, pages = {351 -- 362}, abstract = {With the evolution toward fast networks of many-core processors, the design assumptions at the basis of software-level distributed shared memory (DSM) systems change considerably. But efficient DSMs are needed because they can significantly simplify the implementation of complex distributed algorithms. This paper discusses implications of the many-core evolution and derives a set of reusable elementary operations for future software DSMs. These elementary operations will help in exploring and evaluating new memory models and consistency protocols.}, language = {en} } @techreport{FeindtWoltersBackesetal., author = {Feindt, Peter H. and Wolters, Volkmar and Backes, Gunter and Bahrs, Enno and Brandt, Horst and Dempfle, Leo and Engels, Eve-Marie and Engels, Johannes and Graner, Andreas and Hamm, Ulrich and Herdegen, Matthias and Isselstein, Johannes and Konnert, Monika and Schr{\"o}der, Stefan and Wagner, Sven and W{\"a}tzold, Frank and Wedekind, Helmut}, title = {Verbraucher f{\"u}r die Erhaltung der biologischen Vielfalt in der Landwirtschaft aktivieren!}, publisher = {Bundesanstalt f{\"u}r Landwirtschaft und Ern{\"a}hrung (BLE) Informations- und Koordinationszentrum f{\"u}r Biologische Vielfalt (IBV)}, address = {Bonn}, pages = {12}, language = {de} } @misc{SharatJosephVogeletal., author = {Sharat, S. U. and Joseph, Merin and Vogel, Stefan and Hildebrandt, E. and Komissinskiy, P. and Kurian, J. and Schr{\"o}der, Thomas and Alff, Lambert}, title = {Impact of Oxygen stoichiometry on electroforming and multiple switching modes in TiN/TaOₓ/Pt based ReRAM}, series = {Applied Physics Letters}, volume = {109}, journal = {Applied Physics Letters}, number = {17}, issn = {1077-3118}, doi = {10.1063/1.4965872}, pages = {173503}, language = {en} } @misc{GeorgeRoedigerSchroederetal., author = {George, Sandra and R{\"o}diger, Stefan and Schr{\"o}der, Christian and Knaut, Michael and K{\"u}pper, Jan-Heiner}, title = {Development of multiplex PCR systems for expression profiling of human cardiomyocytes induced to profilerate by lentivirus transduction of upcyte genes}, series = {Journal of Cellular Biotechnology}, volume = {2}, journal = {Journal of Cellular Biotechnology}, number = {1}, issn = {2352-3697}, doi = {10.3233/JCB-15025}, pages = {35 -- 55}, language = {en} } @techreport{FeindtBahrsEngelsetal., author = {Feindt, Peter H. and Bahrs, Enno and Engels, Eve-Marie and Hamm, Ulrich and Herdegen, Matthias and Isselstein, Johannes and Schr{\"o}der, Stefan and W{\"a}tzold, Frank and Wolters, Volkmar}, title = {F{\"u}r eine Gemeinsame Agrarpolitik, die konsequent zum Erhalt der biologischen Vielfalt beitr{\"a}gt}, publisher = {Bundesanstalt f{\"u}r Landwirtschaft und Ern{\"a}hrung (BLE), Informations- und Koordinationszentrum f{\"u}r Biologische Vielfalt (IBV)}, address = {Bonn}, pages = {36}, language = {de} } @misc{SchierackRoedigerKuhletal., author = {Schierack, Peter and R{\"o}diger, Stefan and Kuhl, Christoph and Hiemann, Rico and Roggenbuck, Dirk and Li, Ganwu and Weinreich, J{\"o}rg and Berger, Enrico and Nolan, Lisa K. and Nicholson, Bryon and R{\"o}mer, Antje and Fr{\"o}mmel, Ulrike and Wieler, Lothar H. and Schr{\"o}der, Christian}, title = {Porcine E. coli: Virulence-Associated Genes, Resistance Genes and Adhesion and Probiotic Activity Tested by a New Screening Method}, series = {PLoS One}, volume = {8}, journal = {PLoS One}, number = {4}, doi = {10.1371/journal.pone.0059242}, pages = {13}, abstract = {We established an automated screening method to characterize adhesion of Escherichia coli to intestinal porcine epithelial cells (IPEC-J2) and their probiotic activity against infection by enteropathogenic E. coli (EPEC). 104 intestinal E. coli isolates from domestic pigs were tested by PCR for the occurrence of virulence-associated genes, genes coding for resistances to antimicrobial agents and metals, and for phylogenetic origin by PCR. Adhesion rates and probiotic activity were examined for correlation with the presence of these genes. Finally, data were compared with those from 93 E. coli isolates from wild boars. Isolates from domestic pigs carried a broad variety of all tested genes and showed great diversity in gene patterns. Adhesions varied with a maximum of 18.3 or 24.2 mean bacteria adherence per epithelial cell after 2 or 6 hours respectively. Most isolates from domestic pigs and wild boars showed low adherence, with no correlation between adhesion/probiotic activity and E. coli genes or gene clusters. The gene sfa/foc, encoding for a subunit of F1C fimbriae did show a positive correlative association with adherence and probiotic activity; however E. coli isolates from wild boars with the sfa/foc gene showed less adhesion and probiotic activity than E. coli with the sfa/foc gene isolated from domestic pigs after 6 hour incubation. In conclusion, screening porcine E. coli for virulence associated genes genes, adhesion to intestinal epithelial cells, and probiotic activity revealed a single important adhesion factor, several probiotic candidates, and showed important differences between E. coli of domestic pigs and wild boars.}, language = {en} } @misc{FroemmelLehmannRoedigeretal., author = {Fr{\"o}mmel, Ulrike and Lehmann, Werner and R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Weinreich, J{\"o}rg and Groß, Julia and Roggenbuck, Dirk and Zinke, Olaf and Ansorge, Hermann and Vogel, Steffen and Klemm, Per and Wex, Thomas and Schr{\"o}der, Christian and Wieler, Lothar H. and Schierack, Peter}, title = {Adhesion of human and animal Escherichia coli strains in association with their virulence-associated genes and phylogenetic origins}, series = {Applied and environmental microbiology}, volume = {79}, journal = {Applied and environmental microbiology}, number = {19}, issn = {1098-5336}, doi = {10.1128/AEM.01384-13}, pages = {5814 -- 5829}, abstract = {Intestinal colonization is influenced by the ability of the bacterium to inhabit a niche, which is based on the expression of colonization factors. Escherichia coli carries a broad range of virulence-associated genes (VAGs) which contribute to intestinal (inVAGs) and extraintestinal (exVAGs) infection. Moreover, initial evidence indicates that inVAGs and exVAGs support intestinal colonization. We developed new screening tools to genotypically and phenotypically characterize E. coli isolates originating in humans, domestic pigs, and 17 wild mammal and avian species. We analyzed 317 isolates for the occurrence of 44 VAGs using a novel multiplex PCR microbead assay (MPMA) and for adhesion to four epithelial cell lines using a new adhesion assay. We correlated data for the definition of new adhesion genes. inVAGs were identified only sporadically, particularly in roe deer (Capreolus capreolus) and the European hedgehog ( Erinaceus europaeus). The prevalence of exVAGs depended on isolation from a specific host. Human uropathogenic E. coli isolates carried exVAGs with the highest prevalence, followed by badger (Meles meles) and roe deer isolates. Adhesion was found to be very diverse. Adhesion was specific to cells, host, and tissue, though it was also unspecific. Occurrence of the following VAGs was associated with a higher rate of adhesion to one or more cell lines: afa-dra, daaD, tsh, vat, ibeA, fyuA, mat, sfa-foc, malX, pic, irp2, and papC. In summary, we established new screening methods which enabled us to characterize large numbers of E. coli isolates. We defined reservoirs for potential pathogenic E. coli. We also identified a very broad range of colonization strategies and defined potential new adhesion genes.}, language = {en} } @misc{FroemmelBoehmNitschkeetal., author = {Fr{\"o}mmel, Ulrike and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Weinreich, J{\"o}rg and Groß, Julia and R{\"o}diger, Stefan and Wex, Thomas and Ansorge, Hermann and Zinke, Olaf and Schr{\"o}der, Christian and Roggenbuck, Dirk and Schierack, Peter}, title = {Adhesion patterns of commensal and pathogenic Escherichia coli from humans and wild animals on human and porcine epithelial cell lines}, series = {Gut Pathogens}, volume = {5}, journal = {Gut Pathogens}, number = {31}, issn = {1757-4749}, doi = {10.1186/1757-4749-5-31}, pages = {8}, abstract = {Abstract BACKGROUND: Different strategies of colonization or infection by E. coli result in formation of certain adhesion patterns which help also in classifying intestinal E. coli into pathotypes. Little is known about adhesion patterns and host- and tissue adaption of commensal E. coli and about E. coli originating in clinically healthy hosts carrying pathotype-specific virulence-associated genes. FINDINGS: Adhesion pattern of E. coli (n = 282) from humans and from 18 animal species were verified on intestinal human Caco-2 and porcine IPEC-J2 cells and, furthermore, for comparison on human urinary bladder 5637, porcine kidney PK-15 epithelial and HEp-2 cells. The analysis was carried out on 150,000 images of adhesion assays.Adhesion patterns were very diverse; 88 isolates were completely non-adherent, whereas 194 adhered to at least one cell line with the dominant adhesion patterns "diffusely distributed" and "microcolony formation". Adhesion patterns "chains" and "clumps" were also visible. Chain formation was mediated by the presence of epithelial cells. Clump formation was very specific on only the 5637 cell line. All enteropathogenic (eae+) E. coli (EPEC; n = 14) were able to form microcolonies which was cell line specific for each isolate. Most EPEC formed microcolonies on intestinal IPEC-J2 and Caco-2 but several also on urinary tract cells. Shigatoxin-producing (stx+) E. coli (n = 10) showed no specific adhesion patterns. CONCLUSIONS: E. coli isolates were highly diverse. Commensal and pathogenic isolates can adhere in various forms, including diffuse distribution, microcolonies, chains and clumps. Microcolony formation seems to be a global adhesion strategy also for commensal E. coli.}, language = {en} } @misc{RoedigerRuhlandSchroederetal., author = {R{\"o}diger, Stefan and Ruhland, Mirko and Schr{\"o}der, Christian and Großmann, Kai and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Berger, Ingo and Schimke, Ingolf and Schierack, Peter}, title = {Fluorescence dye adsorption assay to quantify carboxyl groups on the surface of poly(methyl methacrylate) microbeads}, series = {Analytical Chemistry}, volume = {83}, journal = {Analytical Chemistry}, number = {9}, issn = {0003-2700}, doi = {10.1021/ac103277s}, pages = {3379 -- 3385}, language = {en} } @incollection{RoedigerSchierackBoehmetal., author = {R{\"o}diger, Stefan and Schierack, Peter and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Berger, Ingo and Fr{\"o}mmel, Ulrike and Schmidt, Carsten and Ruhland, Mirko and Schimke, Ingolf and Roggenbuck, Dirk and Lehmann, Werner and Schr{\"o}der, Christian}, title = {A highly versatile microscope imaging technology platform for the multiplex real-time detection of biomolecules and autoimmune antibodies}, series = {Molecular diagnostics}, booktitle = {Molecular diagnostics}, editor = {Seitz, Harald and Berger, Ingo}, publisher = {Springer}, address = {Berlin [u.a.]}, isbn = {978-3-642-37690-0}, pages = {35 -- 74}, language = {en} } @misc{SchmidtRoedigerGruneretal., author = {Schmidt, Carsten and R{\"o}diger, Stefan and Gruner, Melanie and Moncsek, Anja and Stohwasser, Ralf and Hanack, Katja and Schierack, Peter and Schr{\"o}der, Christian}, title = {Multiplex localization of sequential peptide epitopes by use of a planar microbead chip}, series = {Analytica Chimica Acta}, volume = {908}, journal = {Analytica Chimica Acta}, issn = {0003-2670}, doi = {10.1016/j.aca.2015.12.030}, pages = {150 -- 160}, language = {en} } @misc{RoedigerKramerFroemmeletal., author = {R{\"o}diger, Stefan and Kramer, Toni and Fr{\"o}mmel, Ulrike and Weinreich, J{\"o}rg and Roggenbuck, Dirk and Guenther, Sebastian and Schaufler, Katharina and Schr{\"o}der, Christian and Schierack, Peter}, title = {Intestinal Escherichia coli colonization in a mallard duck population over four consecutive winter season}, series = {Environmental Microbiology}, volume = {17}, journal = {Environmental Microbiology}, number = {9}, issn = {1462-2920}, doi = {10.1111/1462-2920.12807}, pages = {3352 -- 3361}, language = {en} } @inproceedings{RoedigerBoehmNitschkeetal., author = {R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Stavitskaya, Luba and Gruner, Melanie and Kundzer, Alena V. and Volkova, Margarita V. and Generalov, I. and Schmidt, Carsten and Schr{\"o}der, Christian and Roggenbuck, Dirk and Schierack, Peter}, title = {Development of a Method for Multiplex Real-Time Analysis of Enzymatic Activity on a Microbead-Chip}, series = {Infection, tumors and autoimmunity, report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 1-4, 2013}, booktitle = {Infection, tumors and autoimmunity, report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 1-4, 2013}, publisher = {Pabst Science Publ.}, address = {Lengerich [u.a.]}, isbn = {978-3-89967-881-9}, pages = {275 -- 280}, language = {en} } @misc{RoedigerLehmannFroemmeletal., author = {R{\"o}diger, Stefan and Lehmann, Werner and Fr{\"o}mmel, Ulrike and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Burdukiewicz, Michał and Schr{\"o}der, Christian and Dangla, R{\´e}mi and Droniou, Magali and Schierack, Peter}, title = {The Modi Operandi of the VideoScan Platform for the Detection and Analysis of Nucleic Acids. Life Science Day 2013 \& 17. Leibniz Conference of Advanced Science, Freie Universit{\"a}t Berlin, 24.10.2013}, pages = {1}, language = {en} } @misc{RoedigerSchneiderSaueretal., author = {R{\"o}diger, Stefan and Schneider, Jens and Sauer, Lysann and Reddig, Annika and Schierack, Peter and Roggenbuck, Dirk and Schr{\"o}der, Christian}, title = {A Multiparameter Platform for The Analysis of DNA Double-strand Breaks and Associated Biomarkers after genome Editing Austrian Biomarker Symposium (ASBD) 2016, 10.-11.03.2016, Wien, {\"O}sterreich}, language = {en} } @misc{SauerRoedigerSchneideretal., author = {Sauer, Lysann and R{\"o}diger, Stefan and Schneider, Jens and Schierack, Peter and Roggenbuck, Dirk and Schr{\"o}der, Christian}, title = {Functional Multiparameter Analy sis of Double-Strand Breaks and Associate Biomarkers during Genome Editing, Genome Editing and Gene Modulation Congress 2016, 6.-8.04.2016, Oxford, United Kingdom}, language = {en} } @inproceedings{RoedigerBoehmNitschkeetal., author = {R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Stavitskaya, Luba and Gruner, Melanie and Kundzer, Alena V. and Volkova, Margarita V. and Generalov, I. and Schmidt, Carsten and Schr{\"o}der, Christian and Roggenbuck, Dirk and Schierack, Peter}, title = {Development of a Method for Multiplex Real-Time Analysis of Enzymati c Activity on a Microbead-Chip.}, series = {Infections, Tumors and Autoimmunity, Report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 01. - 04., 2013}, booktitle = {Infections, Tumors and Autoimmunity, Report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 01. - 04., 2013}, publisher = {Pabst Science Publ.}, address = {Lengerich}, isbn = {978-3-89967-881-9}, pages = {280}, language = {en} } @inproceedings{RoedigerHanschmannSteidleetal., author = {R{\"o}diger, Stefan and Hanschmann, Henning and Steidle, Michael and Schr{\"o}der, Christian and Schierack, Peter and Lehmann, Werner}, title = {Detection of Borrelia, Bordetella and HPV with the LoopTag Real-Time PCR Probe System}, series = {International journal of medical microbiology}, volume = {303}, booktitle = {International journal of medical microbiology}, number = {Supplement 1}, issn = {1618-0607}, pages = {12 -- 13}, language = {en} } @techreport{FeindtWoltersBackesetal., author = {Feindt, Peter H. and Wolters, Volkmar and Backes, Gunter and Bahrs, Enno and Brandt, Horst and Engels, Eve-Marie and Engels, Johannes and Graner, Andreas and Hamm, Ulrich and Herdegen, Matthias and Isselstein, Johannes and Schr{\"o}der, Stefan and Tholen, Ernst and Wagner, Sven and W{\"a}tzold, Frank and Wolf, Heino}, title = {Schutz tiergenetischer Ressourcen im Tierseuchenfall - Vorschl{\"a}ge zum Schutz von Tierbest{\"a}nden in Nukleus- bzw. Basiszuchtbetrieben und Besamungsstationen bei Auftreten der Afrikanischen Schweinepest}, publisher = {Bundesanstalt f{\"u}r Landwirtschaft und Ern{\"a}hrung (BLE), Informations- und Koordinationszentrum f{\"u}r Biologische Vielfalt (IBV)}, address = {Bonn}, pages = {9}, language = {de} }