@misc{FriedrichHollensteinerSchneideretal., author = {Friedrich, Ines and Hollensteiner, Jacqueline and Schneider, Dominik and Poehlein, Anja and Hertel, Robert and Daniel, Rolf}, title = {First Complete Genome Sequences of Janthinobacterium lividum EIF1 and EIF2 and their Comparative Genome Analysis}, series = {Genome Biology and Evolution}, volume = {12}, journal = {Genome Biology and Evolution}, number = {10}, issn = {1759-6653}, doi = {10.1093/gbe/evaa148}, pages = {1782 -- 1788}, abstract = {We present the first two complete genomes of the Janthinobacterium lividum species, namely strains EIF1 and EIF2, which both possess the ability to synthesize violacein. The violet pigment violacein is a secondary metabolite with antibacterial, antifungal, antiviral, and antitumoral properties. Both strains were isolated from environmental oligotrophic water ponds in G{\"o}ttingen. The strains were phylogenetically classified by ANI analysis and showed a species assignment to Janthinobacterium lividum with 97.72\% (EIF1) and 97.66\% (EIF2) identity. These are the first complete genome sequences of strains belonging to the species Janthinobacterium lividum. The genome of strain EIF1 consists of one circular chromosome (6,373,589 bp) with a GC-content of 61.98\%. The genome contains 5,551 coding sequences, 122 rRNAs, 93 tRNAs, and 1 tm-RNA. The genome of EIF2 comprises one circular chromosome (6,399,352 bp) with a GC-content of 61.63\% and a circular plasmid p356839 (356,839 bp) with a GC-content of 57.21\%. The chromosome encodes 5,691 coding sequences, 122 rRNAs, 93 tRNAs, and 1 tm-RNA and the plasmid harbors 245 coding sequences. In addition to the highly conserved chromosomally encoded violacein operon, the plasmid comprises a non-ribosomal peptide synthetase cluster (NRPS) with similarity to xenoamicin, which is a bioactive compound effective against protozoan parasites.}, language = {en} } @misc{HertelSchoeneMittelstaedtetal., author = {Hertel, Robert and Sch{\"o}ne, Kerstin and Mittelst{\"a}dt, Carolin and Meißner, Janek and Zschoche, Nick and Collignon, Madeline and Kohler, Christian and Friedrich, Ines and Schneider, Dominik and Hoppert, Michael and Kuhn, Ramona and Schwedt, Inge and Scholz, Patricia and Poehlein, Anja and Martienssen, Marion and Ischebeck, Till and Daniel, Rolf and Commichau, Fabian M.}, title = {Characterization of glyphosate-resistant Burkholderia anthina and Burkholderia cenocepacia isolates from a commercial Roundup® solution}, series = {Environmental Microbiology Reports}, volume = {14}, journal = {Environmental Microbiology Reports}, number = {1}, issn = {1758-2229}, doi = {10.1111/1758-2229.13022}, pages = {70 -- 84}, abstract = {Roundup® is the brand name for herbicide solutions containing glyphosate, which specifically inhibits the 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase of the shikimate pathway. The inhibition of the EPSP synthase causes plant death because EPSP is required for biosynthesis of aromatic amino acids. Glyphosate also inhibits the growth of archaea, bacteria, Apicomplexa, algae and fungi possessing an EPSP synthase. Here, we have characterized two glyphosate-resistant bacteria from a Roundup solution. Taxonomic classification revealed that the isolates 1CH1 and 2CH1 are Burkholderia anthina and Burkholderia cenocepacia strains respectively. Both isolates cannot utilize glyphosate as a source of phosphorus and synthesize glyphosate-sensitive EPSP synthase variants. Burkholderia. anthina 1CH1 and B. cenocepacia 2CH1 tolerate high levels of glyphosate because the herbicide is not taken up by the bacteria. Previously, it has been observed that the exposure of soil bacteria to herbicides like glyphosate promotes the development of antibiotic resistances. Antibiotic sensitivity testing revealed that the only the B. cenocepacia 2CH1 isolate showed increased resistance to a variety of antibiotics. Thus, the adaptation of B. anthina 1CH1 and B. cenocepacia 2CH1 to glyphosate did not generally increase the antibiotic resistance of both bacteria. However, our study confirms the genomic adaptability of bacteria belonging to the genus Burkholderia.}, language = {en} } @misc{FriedrichKlassenNeubaueretal., author = {Friedrich, Ines and Klassen, Anna and Neubauer, Hannes and Schneider, Dominik and Hertel, Robert and Daniel, Rolf}, title = {Living in a Puddle of Mud: Isolation and Characterization of Two Novel Caulobacteraceae Strains Brevundimonas pondensis sp. nov. and Brevundimonas goettingensis sp. nov.}, series = {Applied Microbiology}, volume = {1}, journal = {Applied Microbiology}, number = {1}, issn = {2673-8007}, doi = {10.3390/applmicrobiol1010005}, pages = {38 -- 59}, abstract = {Brevundimonas is a genus of freshwater bacteria belonging to the family Caulobacteraceae. The present study describes two novel species of the genus Brevundimonas (LVF1 T and LVF2 T ). Both were genomically, morphologically, and physiologically characterized. Average nucleotide identity analysis revealed both are unique among known Brevundimonas strains. In silico and additional ProphageSeq analyses resulted in two prophages in the LVF1 T genome and a remnant prophage in the LVF2 T genome. Bacterial LVF1 T cells form an elliptical morphotype, in average 1 µ m in length and 0.46 µ m in width, with a single flagellum. LVF2 T revealed motile cells approximately 1.6 µ m in length and 0.6 µ m in width with a single flagellum, and sessile cell types 1.3 µ m in length and 0.6 µ m in width. Both are Gram-negative, aerobic, have optimal growth at 30 ◦ C (up to 0.5 to 1\% NaCl). Both are resistant towards erythromycin, meropenem, streptomycin, tetracycline and vancomycin. Anaerobic growth was observed after 14 days for LVF1 T only. For LVF1 T the name Brevundimonas pondensis sp. nov. and for LVF2 T the name Brevundimonas goettingensis sp. nov. are proposed. Type strains are LVF1 T (=DSM 112304 T = CCUG 74982 T = LMG 32096 T ) and LVF2 T (=DSM 112305 T = CCUG 74983 T = LMG 32097 T ).}, language = {en} }