@misc{DinterBurdukiewiczSchieracketal., author = {Dinter, Franziska and Burdukiewicz, Michał and Schierack, Peter and Lehmann, Werner and Nestler, J{\"o}rg and Dame, Gregory and R{\"o}diger, Stefan}, title = {Simultaneous detection and quantification of DNA and protein biomarkers in spectrum of cardiovascular diseases in a microfluidic microbead chip}, series = {Analytical and Bioanalytical Chemistry}, volume = {411}, journal = {Analytical and Bioanalytical Chemistry}, number = {29}, issn = {1618-2650}, doi = {10.1007/s00216-019-02199-x}, pages = {7725 -- 7735}, language = {en} } @misc{DeutschmannSowaMurugaiyanetal., author = {Deutschmann, Claudia and Sowa, Mandy and Murugaiyan, Jayaseelan and Roessler, Uwe and R{\"o}ber, Nadja and Conrad, Karsten and Laass, Martin W. and Bogdanos, Dimitrios Petrou and Sipeki, Nora and Papp, Maria and R{\"o}diger, Stefan and Roggenbuck, Dirk and Schierack, Peter}, title = {Identification of Chitinase-3-Like Protein 1 as a Novel Neutrophil Antigenic Target in Crohn's Disease}, series = {Journal of Crohn's and Colitis}, volume = {13}, journal = {Journal of Crohn's and Colitis}, number = {7}, issn = {1876-4479}, doi = {10.1093/ecco-jcc/jjz012}, pages = {894 -- 904}, abstract = {Background and Aims There is an increasing incidence of inflammatory bowel disease [IBD]. Autoimmune responses are involved in the pathophysiology of IBD, but their underlying pathways and target antigens have not yet been fully elucidated. Methods Autoantigenic targets in IBD were identified after separation of whole cell proteins isolated from neutrophils using two-dimensional electrophoresis and matrix assisted laser desorption ionization - time of flight mass spectrometry-based protein identification of the spots that displayed Western blotting signals with anti-neutrophil cytoplasmic antibody-positive sera. The prevalence of IgG, IgA and secretory IgA [sIgA] to chitinase 3-like protein 1 [CHI3L1] was analysed by enzyme-linked immunosorbent assays using recombinant CHI3L1 in 110 patients with Crohn's disease [CD], 95 with ulcerative colitis [UC], 126 with coeliac disease [CeD] and 86 healthy controls [HCs]. Results The 18-glycosylhydrolase family member CHI3L1 was identified as a neutrophil autoantigenic target. CD patients displayed significantly higher levels of IgG to CHI3L1 than patients with UC and CeD (p < 0.0001, respectively). IgA and sIgA to CHI3L1 was significantly higher in CD than in UC, CeD and HCs [p < 0.0001, respectively]. IgA and sIgA to CHI3L1 demonstrated the highest prevalence in CD [25.5\%, 28/110; and 41.8\%\%, 46/110] compared to HCs [2.3\%, 2/86; and 4.7\%\%, 4/86; p = 0.0015 and p < 0.0001] and are associated with a more complicated progression of CD. Conclusion CHI3L1 is a novel neutrophil autoantigenic target in CD. IgA and sIgA to CHI3L1 may serve as novel markers for CD and may facilitate the serological diagnosis of IBD.}, language = {en} } @misc{SowaMurugaiyanConradetal., author = {Sowa, Mandy and Murugaiyan, Jayaseelan and Conrad, Karsten and Laass, Martin W. and Bogdanos, Dimitrios Petrou and Papp, Maria and R{\"o}diger, Stefan and Roggenbuck, Dirk and Schierack, Peter}, title = {A novel neutrophil autoantigenic target in inflammatory bowel disease}, language = {en} } @misc{JurischkaDinterEfimovaetal., author = {Jurischka, Christoph and Dinter, Franziska and Efimova, Anastasia and Weiss, Romano and Schiebel, Juliane and Schulz, Christian and Fayziev, Bekzodjon and Schierack, Peter and Fischer, Thomas and R{\"o}diger, Stefan}, title = {An explorative study of polymers for 3D printing of bioanalytical test systems}, series = {Clinical Hemorheology and Microcirculation}, volume = {75}, journal = {Clinical Hemorheology and Microcirculation}, number = {1}, issn = {1875-8622}, doi = {10.3233/CH-190713}, pages = {57 -- 84}, abstract = {Background: The 3D printing is relevant as a manufacturing technology of functional models for forensic, pharmaceutical and bioanalytical applications such as drug delivery systems, sample preparation and point-of-care tests. Objective: Melting behavior and autofluorescence of materials are decisive for optimal printing and applicability of the product which are influenced by varying unknown additives. Methods: We have produced devices for bioanalytical applications from commercially available thermoplastic polymers using a melt-layer process. We characterized them by differential scanning calorimetry, fluorescence spectroscopy and functional assays (DNA capture assay, model for cell adhesion, bacterial adhesion and biofilm formation test). Results: From 14 tested colored, transparent and black materials we found only deep black acrylonitrile-butadiene-styrene (ABS) and some black polylactic acid (PLA) useable for fluorescence-based assays, with low autofluorescence only in the short-wave range of 300-400 nm. PLA was suitable for standard bioanalytical purposes due to a glass transition temperature of approximately 60°C, resistance to common laboratory chemicals and easy print processing. For temperature-critical methods, such as hybridization reactions up to 90°C, ABS was better suited. Conclusions: Autofluorescence was not a disadvantage per se but can also be used as a reference signal in assays. The rapid development of individual protocols for sample processing and analysis required the availability of a material with consistent quality over time. For fluorescence-based assays, the use of commercial standard materials did not seem to meet this requirement.}, language = {en} } @misc{LopensKrawczykPappetal., author = {Lopens, Steffi and Krawczyk, Marcin and Papp, Maria and Milkiewicz, Piotr and Schierack, Peter and Liu, Yudong and Wunsch, Ewa and Conrad, Karsten and Roggenbuck, Dirk}, title = {The search for the Holy Grail: autoantigenic targets in primary sclerosing cholangitis asscociated with diesease phenotype an neoplasia}, series = {Autoimmunity Highlights}, volume = {11}, journal = {Autoimmunity Highlights}, issn = {2038-3274}, doi = {10.1186/s13317-020-00129-x}, pages = {14}, language = {en} } @misc{SchmidtSchierackGerberetal., author = {Schmidt, Carsten and Schierack, Peter and Gerber, Ulrike and Schr{\"o}der, Christian and Choi, Youngeun and Bald, Ilko and Lehmann, Werner and R{\"o}diger, Stefan}, title = {Streptavidin Homologues for Applications on Solid Surfaces at High Temperatures}, series = {Langmuir}, volume = {36}, journal = {Langmuir}, number = {2}, issn = {1520-5827}, doi = {10.1021/acs.langmuir.9b02339}, pages = {628 -- 636}, language = {en} } @misc{VolkovSeguroLeionetal., author = {Volkov, Ilan and Seguro, Luciana and Leion, Elaine P. and Kov{\´a}cs, L{\´a}szl{\´o} and Roggenbuck, Dirk and Schierack, Peter and Gilburd, Boris and Doria, Andrea and Tektonidou, Maria G. and Agmon-Levin, Nancy}, title = {Profiles of criteria and non-criteria anti-phospholipid autoantibodies are associated with clinical phenotypes of the antiphospholipid syndrome}, series = {Autoimmunity Highlights}, volume = {11}, journal = {Autoimmunity Highlights}, issn = {2038-3274}, doi = {10.1186/s13317-020-00131-3}, pages = {1 -- 8}, language = {en} } @misc{AliKolendaKhanetal., author = {Ali, Aamir and Kolenda, Rafał and Khan, Muhammad Moman and Weinreich, J{\"o}rg and Li, Ganwu and Wieler, Lothar H. and Tedin, Karsten and Roggenbuck, Dirk and Schierack, Peter}, title = {Novel Avian Pathogenic Escherichia coli Genes Responsible for Adhesion to Chicken and Human Cell Lines}, series = {Applied and Environmental Microbiology}, volume = {86}, journal = {Applied and Environmental Microbiology}, number = {20}, issn = {1098-5336}, doi = {10.1128/AEM.01068-20}, language = {en} } @misc{SchierackHeidenKhanetal., author = {Schierack, Peter and Heiden, Stefan E. and Khan, Muhammad Moman and Nikolaus, Lena and Kolenda, Rafał and Stubbe, Michael and Lkhagvasuren, Davaa and R{\"o}diger, Stefan and Guenther, Sebastian and Schaufler, Katharina}, title = {Genomic and Phenotypic Analysis of an ESBL-Producing E. coli ST1159 Clonal Lineage From Wild Birds in Mongolia}, series = {Frontiers in Microbiology}, volume = {11}, journal = {Frontiers in Microbiology}, issn = {1664-302X}, doi = {10.3389/fmicb.2020.01699}, pages = {7}, language = {en} } @misc{ReimannZengJakopecetal., author = {Reimann, Ronny and Zeng, Bo and Jakopec, Martin and Burdukiewicz, Michał and Petrick, Ingolf and Schierack, Peter and R{\"o}diger, Stefan}, title = {Classification of dead and living microalgae Chlorella vulgaris by bioimage informatics an machine learning}, series = {Algal Research}, volume = {48}, journal = {Algal Research}, issn = {2211-9264}, doi = {10.1016/j.algal.2020.101908}, pages = {11}, language = {en} } @misc{RoggenbuckElmontReinholdetal., author = {Roggenbuck, Dirk and Elmont, Emilien and Reinhold, Dirk and Schierack, Peter and Conrad, Karsten and Boucraut, Joseph}, title = {Autoimmune Perpheral Neuropathies and Contribution of Antiganglioside/Sulphatide Autoantibody Testing}, series = {Mediterranean Jounal of Rheumatology}, volume = {31}, journal = {Mediterranean Jounal of Rheumatology}, number = {1}, issn = {2529-198X}, doi = {10.31138/mjr.31.1.10}, pages = {10 -- 18}, language = {en} } @misc{DeutschmannRoggenbuckSchieracketal., author = {Deutschmann, Claudia and Roggenbuck, Dirk and Schierack, Peter and R{\"o}diger, Stefan}, title = {Autoantibody testing by enzyme-linked immunosorbent assay-a case in which the solid phase decides on success and failure}, series = {Heliyon}, volume = {6}, journal = {Heliyon}, number = {1}, issn = {2405-8440}, doi = {10.1016/j.heliyon.2020.e03270}, pages = {6}, language = {en} } @misc{SchiebelNoackRoedigeretal., author = {Schiebel, Juliane and Noack, Jonas and R{\"o}diger, Stefan and Kammel, Anne and Menzel, Friederike and Schwibbert, Karin and Weise, Matthias and Weiss, Romano and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Elimport, Alexey and Roggenbuck, Dirk and Schierack, Peter}, title = {Analysis of three-dimensional biofilms on different material surfaces}, series = {Biomaterials Science}, volume = {8}, journal = {Biomaterials Science}, number = {12}, issn = {2047-4849}, doi = {10.1039/D0BM00455C}, pages = {3500 -- 3510}, language = {en} } @misc{LiedtkeSchroederRoggenbucketal., author = {Liedtke, Victoria and Schr{\"o}der, Christian and Roggenbuck, Dirk and Weiss, Romano and Stohwasser, Ralf and Schierack, Peter and R{\"o}diger, Stefan and Schenk, Lysann}, title = {LEDGF/p75 is required for an efficient DNA damage response}, series = {International Journal of Molecular Sciences}, volume = {22}, journal = {International Journal of Molecular Sciences}, number = {11}, doi = {10.3390/ijms22115866}, pages = {1 -- 16}, language = {en} } @misc{KolendaBurdukiewiczWimonćetal., author = {Kolenda, Rafał and Burdukiewicz, Michał and Wimonć, Marcjanna and Aleksandrowicz, Adrianna and Ali, Aamir and Szabo, Istvan and Tedin, Karsten and Bartholdson, Scott J. and Pickhard, Derek and Schierack, Peter}, title = {Identification of Natural Mutations Responsible for Altered Infection Phenotypes of Salmonella enterica Clinical Isolates by Using Cell Line Infection Screens}, series = {Applied and Environmental Microbiology}, volume = {87}, journal = {Applied and Environmental Microbiology}, number = {2}, issn = {1098-5336}, doi = {10.1128/AEM.02177-20}, language = {en} } @misc{ComabellaDeutschmannMidagliaetal., author = {Comabella, Manual and Deutschmann, Claudia and Midaglia, Luciana and Schierack, Peter and Mart{\´i}nez, J{\´u}lia and Roggenbuck, Dirk and Montalban, Xavier}, title = {Chitinase 3-like 1 is not a target antigen in patients with multiple sclerosis}, series = {Multiple Sclerosis Journal}, volume = {27}, journal = {Multiple Sclerosis Journal}, number = {9}, issn = {1477-0970}, doi = {10.1177/1352458520980141}, pages = {1455 -- 1457}, language = {en} } @misc{SchmidtBerghausBlessingetal., author = {Schmidt, Jonas and Berghaus, Sandro and Blessing, Frithjof and Wenzel, Folker and Herbeck, Holger and Blessing, Josef and Schierack, Peter and R{\"o}diger, Stefan and Roggenbuck, Dirk}, title = {Serological and viral genetic features of patients with COVID-19 in a selected German patient cohort-correlation with disease characteristics}, series = {GeroScience}, volume = {43}, journal = {GeroScience}, number = {5}, issn = {2509-2723}, doi = {10.1007/s11357-021-00443-w}, pages = {2249 -- 2264}, language = {en} } @misc{AdefioyeWeinreichRoedigeretal., author = {Adefioye, Olusolabomi J. and Weinreich, J{\"o}rg and R{\"o}diger, Stefan and Schierack, Peter and Olowe, Olugbenga Adekunle}, title = {Phylogenetic Characterization and Multilocus Sequence Typing of Extended-Spectrum Beta Lactamase-Producing Escherichia coli from Food-Producing Animals, Beef, and Humans in Southwest Nigeria}, series = {Microbial Drug Resistance}, volume = {27}, journal = {Microbial Drug Resistance}, number = {1}, issn = {1931-8448}, doi = {10.1089/mdr.2019.0397}, pages = {111 -- 120}, language = {en} } @misc{HanschmannRoedigerKrameretal., author = {Hanschmann, Henning and R{\"o}diger, Stefan and Kramer, Toni and Hanschmann, Katrin and Steidle, Michael and Fingerle, Volker and Schmidt, Carsten and Lehmann, Werner and Schierack, Peter}, title = {LoopTag FRET Probe System for Multiplex qPCR Detection of Borrelia Species}, series = {Life}, volume = {11}, journal = {Life}, number = {11}, issn = {2075-1729}, doi = {10.3390/life11111163}, language = {en} } @misc{FotangBroeringRoosetal., author = {Fotang, Chefor and Br{\"o}ring, Udo and Roos, Christian and Enoguanbhor, Evidence Chinedu and E. Abwe, Ekwoge and Dutton, Paul and Schierack, Peter and Angwafo, Tsi Evaristus and Birkhofer, Klaus}, title = {Human activity and forest fragmentation threaten populations of the Nigeria-Cameroon Chimpanzee (Pan troglodytes ellioti) in Western Cameroon}, series = {International Journal of Primatology}, volume = {42}, journal = {International Journal of Primatology}, number = {1}, issn = {1573-8604}, doi = {10.1007/s10764-020-00191-2}, pages = {105 -- 129}, abstract = {Increased human activities such as commodity-led deforestation, extension of agriculture, urbanization, and wildfires are major drivers of forest loss worldwide. In Cameroon, these activities cause a loss of suitable primate habitat and could ultimately threaten the survival of chimpanzees (Pan troglodytes). We derived independent estimates of the population size of the Endangered Nigeria-Cameroon chimpanzee (Pan troglodytes ellioti) in Kom-Wum Forest Reserve, Cameroon, and surrounding unprotected forest areas through 1) direct observations, 2) camera trapping, 3) distance sampling, 4) marked nest counts, and 5) standing crop nest counts. In addition, we georeferenced signs of chimpanzee and human activity along line transects. We used a generalized linear mixed model to predict the occurrence of chimpanzees in response to edge length (measured as the perimeter of core forest patches), core area of forest patches (measured as area of forest patches beyond an edge width of 100 m), habitat perforation (measured as the perimeter of nonforested landscape within core forest patches), patch size(measured as area of forest patches), and forest cover. Chimpanzee density estimates ranged from 0.1 (direct observation) to 0.9 (distance sampling) individuals km-2 depending on estimation method with a mean nest group size of 7 ± 5.4 (SD). The mean encounter rate for signs of chimpanzee activity was significantly higher in mature forests (2.3 signs km-1) than in secondary forests (0.3 signs km-1) and above 1000 m elevation (4.0 signs km-1) than below 1000 m (1.0 signs km-1). The mean encounter rate for signs of human activity was significantly higher in secondary (8.0 signs km-1) than in mature forests (0.9 signs km-1). Secondary forests, habitat perforation, and edge length had a significant negative effect on the occurrence of chimpanzee signs. Overall, human activity and forest degradation affected the number of observed chimpanzee signs negatively. Regular antipoaching patrols and reforestation programs in degraded areas could potentially reduce threats to populations of endangered species and may increase suitable habitat area.}, language = {en} } @misc{SchmidtBorcherdingThieleetal., author = {Schmidt, Carsten and Borcherding, Heike and Thiele, Thomas and Schedler, Uwe and Werner, Franziska and R{\"o}diger, Stefan and Roggenbuck, Dirk and Schierack, Peter}, title = {Fluorescence-encoded poly (methyl metharcylate) nanoparticles for a lateral flow assay detecting IgM autoantibodies in rheumatoid arthritis}, series = {Analytical biochemistry}, volume = {Vol. 633}, journal = {Analytical biochemistry}, issn = {1096-0309}, doi = {10.1016/journal.ppat.1010118}, language = {en} } @misc{EmmeneggerKumarEmmeneggeretal., author = {Emmenegger, Marc and Kumar, Sreedhar Saseendran and Emmenegger, Vishalini and Malinauskas, Tomas and B{\"u}ttner, Thomas and Rose, Laura and Schierack, Peter and Sprinzl, Martin F. and Sommer, Clemens J. and Lackner, Karl J. and Aguzzi, Adriano and Roggenbuck, Dirk and Frauenknecht, Katrin B. M.}, title = {Anti-prothrombin autoantibodies enriched after infection with SARS-CoV-2 and influenced by strength of antibody response against SARS-CoV-2 proteins}, series = {PLoS pathogens}, volume = {17}, journal = {PLoS pathogens}, number = {12}, issn = {1553-7374}, doi = {10.1371/journal.ppat.1010118}, language = {en} } @misc{AwanYanSarwaretal., author = {Awan, Asad Bashir and Yan, Aixin and Sarwar, Yasra and Schierack, Peter and Ali, Aamir}, title = {Detection of synergistic antimicrobial resistance mechanisms in clinical isolates of Pseudomonas aeruginosa from post-operative wound infections}, series = {Applied Microbiology and Biotechnology}, volume = {105}, journal = {Applied Microbiology and Biotechnology}, number = {2}, issn = {1432-0614}, doi = {10.1007/s00253-021-11680-6}, pages = {9321 -- 9332}, language = {en} } @misc{RoggenbuckZarskeSchieracketal., author = {Roggenbuck, Johannes J. and Zarske, Grit and Schierack, Peter and Wunderlich, Gerd and Conrad, Karsten and Kotzerke, Joerg and Roggenbuck, Dirk and Z{\"o}phel, Klaus}, title = {Third generation radioimmunoassay (RIA) for TSH receptor autoantibodies (TRAb) - one step less, similar results?}, series = {Nuklearmedizin}, volume = {60}, journal = {Nuklearmedizin}, number = {1}, issn = {0029-5566}, doi = {10.1055/a-1277-5972}, pages = {38 -- 46}, language = {en} } @misc{SchmidtBerghausBlessingetal., author = {Schmidt, Jonas and Berghaus, Sandro and Blessing, Frithjof and Wenzel, Folker and Herbeck, Holger and Blessing, Josef and Schierack, Peter and R{\"o}diger, Stefan and Roggenbuck, Dirk}, title = {A semi-automated, isolation-free, high-throughput SARS-CoV-2 reverse transcriptase (RT) loop-mediated isothermal amplification (LAMP) test}, series = {Scientific reports}, volume = {11}, journal = {Scientific reports}, number = {1}, issn = {2045-2322}, doi = {10.1038/s41598-021-00827-0}, language = {en} } @misc{BartlitzKolendaChilimoniuketal., author = {Bartlitz, Christin and Kolenda, Rafał and Chilimoniuk, Jarosław and Grzymajlo, Krzysztof and R{\"o}diger, Stefan and Bauerfeind, Rolf and Ali, Aamir and Tchesnokovag, Veronika and Roggenbuck, Dirk and Schierack, Peter}, title = {Adhesion of Enteropathogenic, Enterotoxigenic, and Commensal Escherichia coli to the Major Zymogen Granule Membrane Glyoprotein 2}, series = {Applied abd Environmental Microbiology}, volume = {88}, journal = {Applied abd Environmental Microbiology}, number = {5}, issn = {1098-5536}, doi = {10.1128/aem.02279-21}, language = {en} } @misc{SchmidtKammelTanneretal., author = {Schmidt, Carsten and Kammel, Anne and Tanner, Julian A. and Kinghorn, Andrew B. and Khan, Muhammad Moman and Lehmann, Werner and Menger, Marcus and Schedler, Uwe and Schierack, Peter and R{\"o}diger, Stefan}, title = {A Multiparametic Fluorescence Assay for Screening Aptamer-Protein Interactions Based on Microbeads}, series = {Scientific Reports}, volume = {12}, journal = {Scientific Reports}, issn = {2045-2322}, doi = {10.1038/s41598-022-06817-0}, pages = {10}, language = {en} }