@misc{RoggenbuckReinholdBaumgartetal., author = {Roggenbuck, Dirk and Reinhold, Dirk and Baumgart, Daniel C. and Schierack, Peter and Conrad, Karsten and Laass, Martin W.}, title = {Autoimmunity in Crohn's Disease-A Putative Stratification Factor of the Clinical Phenotype}, series = {Advances in Clinical Chemistry}, volume = {77}, journal = {Advances in Clinical Chemistry}, issn = {0065-2423}, doi = {10.1016/bs.acc.2016.06.002}, pages = {77 -- 101}, language = {en} } @misc{SowaTrezziHiemannetal., author = {Sowa, Mandy and Trezzi, Barbara and Hiemann, Rico and Schierack, Peter and Großmann, Kai and Scholz, Juliane and Somma, Valentina and Sinico, Renato Alberto and Roggenbuck, Dirk and Radice, Antonella}, title = {Simultaneous comprehensive multiplex autoantibody analysis for rapidly progressive glomerulonephritis}, series = {Medicine}, volume = {95}, journal = {Medicine}, number = {44}, issn = {0025-7974}, doi = {10.1097/MD.0000000000005225}, pages = {5225}, language = {en} } @misc{MohsinRazaRoschanskietal., author = {Mohsin, Mashkoor and Raza, Shahbaz and Roschanski, Nicole and Guenther, Sebastian and Ali, Aamir and Schierack, Peter}, title = {Description of the First Escherichia coli Clinical Isolate Harboring the Colistin Resistance Gene mcr-1 from the Indian Subcontinent}, series = {Antimicrobial Agents and Chemotherapy}, volume = {61}, journal = {Antimicrobial Agents and Chemotherapy}, number = {1}, issn = {1098-6596}, doi = {10.1128/AAC.01945-16}, pages = {2}, language = {en} } @misc{SowaHiemannSchieracketal., author = {Sowa, Mandy and Hiemann, Rico and Schierack, Peter and Reinhold, Dirk and Conrad, Karsten and Roggenbuck, Dirk}, title = {Next-Generation Autoantibody Testing by Combination of Screening and Confirmation-the CytoBead® Technology}, series = {Clinical Reviews in Allergy \& Immunology}, volume = {53}, journal = {Clinical Reviews in Allergy \& Immunology}, number = {1}, issn = {1559-0267}, doi = {10.1007/s12016-016-8574-3}, pages = {87 -- 104}, language = {en} } @misc{KolendaBurdukiewiczSchierack, author = {Kolenda, Rafał and Burdukiewicz, Michał and Schierack, Peter}, title = {A systematic review and meta-analysis of the epidemiology of pathogenic Escherichia coli of calves and the role of calves as reservoirs for human pathogenic E. coli}, series = {Frontiers in Cellular and Infection Microbiology}, volume = {5}, journal = {Frontiers in Cellular and Infection Microbiology}, issn = {2235-2988}, doi = {10.3389/fcimb.2015.00023}, pages = {12}, language = {en} } @misc{RoedigerKramerFroemmeletal., author = {R{\"o}diger, Stefan and Kramer, Toni and Fr{\"o}mmel, Ulrike and Weinreich, J{\"o}rg and Roggenbuck, Dirk and Guenther, Sebastian and Schaufler, Katharina and Schr{\"o}der, Christian and Schierack, Peter}, title = {Intestinal Escherichia coli colonization in a mallard duck population over four consecutive winter season}, series = {Environmental Microbiology}, volume = {17}, journal = {Environmental Microbiology}, number = {9}, issn = {1462-2920}, doi = {10.1111/1462-2920.12807}, pages = {3352 -- 3361}, language = {en} } @misc{MurugaiyanKruegerRoesleretal., author = {Murugaiyan, Jayaseelan and Kr{\"u}ger, Karolin and R{\"o}sler, Uwe and Weinreich, J{\"o}rg and Schierack, Peter}, title = {Assessment of species and antimicrobial resistance among Enterobacteriaceae isolated from mallard duck faeces}, series = {Environmental Monitoring and Assessment}, volume = {187}, journal = {Environmental Monitoring and Assessment}, number = {3}, issn = {0167-6369}, doi = {10.1007/s10661-015-4346-4}, pages = {127 -- 137}, language = {en} } @misc{LodemannStrahlendorfSchieracketal., author = {Lodemann, Ulrike and Strahlendorf, Julia and Schierack, Peter and Klingspor, Shanti and Aschenbach, J{\"o}rg R. and Martens, Holger}, title = {Effects of the Probiotic Enterococcus faecium and Pathogenic Escherichia coli Strains in a Pig and Human Epithelial Intestinal Cell Model}, series = {Scientifica}, volume = {2015}, journal = {Scientifica}, number = {235184}, doi = {10.1155/2015/235184}, pages = {10}, language = {en} } @misc{VillaltaMytilinaiouElsneretal., author = {Villalta, Danilo and Mytilinaiou, Maria G. and Elsner, Martina and Hentschel, Christian and Cuccato, Juliane and Somma, Valentina and Schierack, Peter and Roggenbuck, Dirk and Bogdanos, Dimitrios Petrou}, title = {Autoantibodies to asialoglycoprotein receptor (ASGPR) in patients with autoimmune liver diseases}, series = {Clinica Chimica Acta}, volume = {450}, journal = {Clinica Chimica Acta}, issn = {0009-8981}, doi = {10.1016/j.cca.2015.07.021}, pages = {5}, language = {en} } @misc{KolendaSchierackZiebaetal., author = {Kolenda, Rafał and Schierack, Peter and Zieba, Filip and Zwijacz-Kozica, Tomasz and Bednarski, Michal}, title = {First molecular characterization of Sarcocystis tenella in Tatra chamois (Rupicapra rupicapra tatrica) in Poland}, series = {Parasitology Research}, volume = {114}, journal = {Parasitology Research}, number = {10}, issn = {0932-0113}, doi = {10.1007/s00436-015-4619-4}, pages = {3885 -- 3892}, language = {en} } @misc{RoedigerBurdukiewiczBlagodatskikhetal., author = {R{\"o}diger, Stefan and Burdukiewicz, Michał and Blagodatskikh, Konstantin A. and Jahn, Michael and Schierack, Peter}, title = {R as an Environment for the Reproducible Analysis of DNA Amplification Experiments}, series = {The R journal}, volume = {7}, journal = {The R journal}, number = {1}, issn = {2073-4859}, pages = {127 -- 150}, language = {en} } @misc{RoedigerBurdukiewiczSchierack, author = {R{\"o}diger, Stefan and Burdukiewicz, Michał and Schierack, Peter}, title = {chipPCR: an R package to pre-process raw data of amplification curves. Bioinformatics}, series = {Bioinformatics}, volume = {31}, journal = {Bioinformatics}, number = {17}, issn = {1367-4803}, doi = {10.1093/bioinformatics/btv205}, pages = {2900 -- 2902}, language = {en} } @inproceedings{RoedigerBoehmNitschkeetal., author = {R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Stavitskaya, Luba and Gruner, Melanie and Kundzer, Alena V. and Volkova, Margarita V. and Generalov, I. and Schmidt, Carsten and Schr{\"o}der, Christian and Roggenbuck, Dirk and Schierack, Peter}, title = {Development of a Method for Multiplex Real-Time Analysis of Enzymatic Activity on a Microbead-Chip}, series = {Infection, tumors and autoimmunity, report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 1-4, 2013}, booktitle = {Infection, tumors and autoimmunity, report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 1-4, 2013}, publisher = {Pabst Science Publ.}, address = {Lengerich [u.a.]}, isbn = {978-3-89967-881-9}, pages = {275 -- 280}, language = {en} } @misc{RoedigerLehmannFroemmeletal., author = {R{\"o}diger, Stefan and Lehmann, Werner and Fr{\"o}mmel, Ulrike and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Burdukiewicz, Michał and Schr{\"o}der, Christian and Dangla, R{\´e}mi and Droniou, Magali and Schierack, Peter}, title = {The Modi Operandi of the VideoScan Platform for the Detection and Analysis of Nucleic Acids. Life Science Day 2013 \& 17. Leibniz Conference of Advanced Science, Freie Universit{\"a}t Berlin, 24.10.2013}, pages = {1}, language = {en} } @misc{KramerSchiebelZelcketal., author = {Kramer, Toni and Schiebel, Juliane and Zelck, Claudia and Weinreich, J{\"o}rg and Schierack, Peter and R{\"o}diger, Stefan}, title = {Genotyping and gene expression analysis of putative reference genes in Enterobacteriaceae, 67th Annual Meeting of the German Society of Hygiene and Microbiology (DGHM), 27.-30.09.2015, M{\"u}nster}, doi = {10.7490/f1000research.1111354.1}, language = {en} } @inproceedings{RoedigerBurdukiewiczHeiserichetal., author = {R{\"o}diger, Stefan and Burdukiewicz, Michał and Heiserich, Lisa and Schierack, Peter and Roggenbuck, Dirk}, title = {Digital Enumeration of Double Strand Breaks via γH2AX and Aassociated biomarkers in a Computing Environment for Reproducible Research}, series = {From autoantibody research to standardized diagnostic assays in the management of human diseases, report on the 12th Dresden Symposium on Autoantibodies, September 23-26, 2015}, booktitle = {From autoantibody research to standardized diagnostic assays in the management of human diseases, report on the 12th Dresden Symposium on Autoantibodies, September 23-26, 2015}, publisher = {Pabst Science Publishers}, address = {Lengerich}, isbn = {978-3-95853-104-8}, pages = {S. 413}, language = {en} } @misc{RoedigerSchneiderSaueretal., author = {R{\"o}diger, Stefan and Schneider, Jens and Sauer, Lysann and Reddig, Annika and Schierack, Peter and Roggenbuck, Dirk and Schr{\"o}der, Christian}, title = {A Multiparameter Platform for The Analysis of DNA Double-strand Breaks and Associated Biomarkers after genome Editing Austrian Biomarker Symposium (ASBD) 2016, 10.-11.03.2016, Wien, {\"O}sterreich}, language = {en} } @misc{SauerRoedigerSchneideretal., author = {Sauer, Lysann and R{\"o}diger, Stefan and Schneider, Jens and Schierack, Peter and Roggenbuck, Dirk and Schr{\"o}der, Christian}, title = {Functional Multiparameter Analy sis of Double-Strand Breaks and Associate Biomarkers during Genome Editing, Genome Editing and Gene Modulation Congress 2016, 6.-8.04.2016, Oxford, United Kingdom}, language = {en} } @inproceedings{KolendaBurdukiewiczSzaboetal., author = {Kolenda, Rafał and Burdukiewicz, Michał and Szabo, M. and Orlowska, Aleksandra and Nitschke, J{\"o}rg and B{\"o}hm, Alexander and Schierack, Peter}, title = {Influence of Salmonella FimH sequence variation on binding to pancreatic secretory granule membrane major glycoprotein GP2 of human and porcine origin}, series = {5th ASM Conference on Salmonella, Potsdam, 29.08-01.09.2016}, booktitle = {5th ASM Conference on Salmonella, Potsdam, 29.08-01.09.2016}, publisher = {American Society for Microbiology}, address = {Washington, DC}, pages = {S. 125}, language = {en} } @inproceedings{BurdukiewiczMikolajczykSzaboetal., author = {Burdukiewicz, Michał and Mikolajczyk, A. and Szabo, Istvan and Nitschke, J{\"o}rg and B{\"o}hm, Alexander and Schierack, Peter and Kolenda, Rafał}, title = {Automated enumeration of Salmonella spp. in cell line infection assays and screening of cell line infectivity among various Salmonella isolates}, series = {5th ASM Conference on Salmonella, Potsdam, 29.08-01.09.2016}, booktitle = {5th ASM Conference on Salmonella, Potsdam, 29.08-01.09.2016}, publisher = {American Society for Microbiology}, address = {Washington, DC}, pages = {S. 127}, language = {en} } @inproceedings{KolendaBurdukiewiczSchiebeletal., author = {Kolenda, Rafał and Burdukiewicz, Michał and Schiebel, Juliane and Szabo, Istvan and Orlowska, Aleksandra and Nitschke, J{\"o}rg and B{\"o}hm, Alexander and Schierack, Peter}, title = {Adhesion of Salmonella to pancreatic secretory granule membrane major glycoprotein GP2 depends on FimH sequence variation}, series = {Abstractbook, Microbiology and Infection, 5th Joint Conference of the DGHM \& VAAM, VAAM Annual Meeting 2017, 69th Annual Meeting of the DGHM}, booktitle = {Abstractbook, Microbiology and Infection, 5th Joint Conference of the DGHM \& VAAM, VAAM Annual Meeting 2017, 69th Annual Meeting of the DGHM}, publisher = {Spektrumverlag}, address = {Heidelberg}, pages = {S. 118}, language = {en} } @misc{DeutschmannDinterRoedigeretal., author = {Deutschmann, Claudia and Dinter, Franziska and R{\"o}diger, Stefan and Roggenbuck, Dirk and Schierack, Peter}, title = {Comparison of Lab and Point of Care (POC) technologies - case study for CHI3L1, Potsdam Days on Bioanalysis}, pages = {1}, language = {en} } @inproceedings{RoedigerBoehmNitschkeetal., author = {R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Stavitskaya, Luba and Gruner, Melanie and Kundzer, Alena V. and Volkova, Margarita V. and Generalov, I. and Schmidt, Carsten and Schr{\"o}der, Christian and Roggenbuck, Dirk and Schierack, Peter}, title = {Development of a Method for Multiplex Real-Time Analysis of Enzymati c Activity on a Microbead-Chip.}, series = {Infections, Tumors and Autoimmunity, Report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 01. - 04., 2013}, booktitle = {Infections, Tumors and Autoimmunity, Report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 01. - 04., 2013}, publisher = {Pabst Science Publ.}, address = {Lengerich}, isbn = {978-3-89967-881-9}, pages = {280}, language = {en} } @inproceedings{RoedigerHanschmannSteidleetal., author = {R{\"o}diger, Stefan and Hanschmann, Henning and Steidle, Michael and Schr{\"o}der, Christian and Schierack, Peter and Lehmann, Werner}, title = {Detection of Borrelia, Bordetella and HPV with the LoopTag Real-Time PCR Probe System}, series = {International journal of medical microbiology}, volume = {303}, booktitle = {International journal of medical microbiology}, number = {Supplement 1}, issn = {1618-0607}, pages = {12 -- 13}, language = {en} } @misc{DinterDeutschmannSchieracketal., author = {Dinter, Franziska and Deutschmann, Claudia and Schierack, Peter and Dame, Gregory and R{\"o}diger, Stefan}, title = {Integration of Cardiovascular Disease Biomarkers in a Microfluidic Microbead Chip}, pages = {1}, language = {en} } @misc{SchiebelBoehmNitschkeetal., author = {Schiebel, Juliane and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Burdukiewicz, Michał and Weinreich, J{\"o}rg and Ali, Aamir and Roggenbuck, Dirk and R{\"o}diger, Stefan and Schierack, Peter}, title = {Genotypic and phenotypic characteristics in association with biofilm formation in different pathotypes of human clinical Escherichia coli isolates}, series = {Applied and Environmental Microbiology}, volume = {83}, journal = {Applied and Environmental Microbiology}, number = {24}, issn = {1098-5336}, doi = {10.1128/AEM.01660-17}, language = {en} } @misc{LiebschRoedigerBoehmetal., author = {Liebsch, Claudia and R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Weinreich, J{\"o}rg and Fruth, Angelika and Roggenbuck, Dirk and Lehmann, Werner and Schedler, Uwe and Juretzek, Thomas and Schierack, Peter}, title = {Solid-phase microbead array for multiplex O-serotyping of Escherichia coli}, series = {Microchimica Acta}, volume = {184}, journal = {Microchimica Acta}, number = {5}, issn = {0026-3672}, doi = {10.1007/s00604-017-2088-4}, pages = {1405 -- 1415}, language = {en} } @misc{BurdukiewiczSpiessBlagodatskikhetal., author = {Burdukiewicz, Michał and Spiess, Andrej-Nikolai and Blagodatskikh, Konstantin A. and Lehmann, Werner and Schierack, Peter and R{\"o}diger, Stefan}, title = {Algorithms for automated detection of hook effect-bearing amplification curves}, series = {Biomolecular Detection and Quantification}, volume = {16}, journal = {Biomolecular Detection and Quantification}, issn = {2214-7535}, doi = {10.1016/j.bdq.2018.08.001}, pages = {1 -- 4}, language = {en} } @misc{DeutschmannRoggenbuckSchierack, author = {Deutschmann, Claudia and Roggenbuck, Dirk and Schierack, Peter}, title = {The loss of tolerance to CHI3L1-A putative role in inflammatory bowel disease?}, series = {Clinical Immunology}, volume = {199}, journal = {Clinical Immunology}, issn = {1521-6616}, doi = {10/gftf2k}, pages = {12 -- 17}, language = {en} } @misc{ReddigRuebeRoedigeretal., author = {Reddig, Annika and R{\"u}be, Claudia E. and R{\"o}diger, Stefan and Schierack, Peter and Reinhold, Dirk and Roggenbuck, Dirk}, title = {DNA damage assessment and potential applications in laboratory diagnostics and precision medicine}, series = {Journal of Laboratory and Precision Medicine}, volume = {3}, journal = {Journal of Laboratory and Precision Medicine}, issn = {2519-9005}, doi = {10.21037/jlpm.2018.03.06}, pages = {15}, language = {en} } @misc{SowaReddigSchieracketal., author = {Sowa, Mandy and Reddig, Annika and Schierack, Peter and Reinhold, Dirk and Roggenbuck, Dirk}, title = {Phosphorylated histone 2AX foci determination in capillary blood mononuclear cells}, series = {Journal of Laboratory and Precision Medicine}, volume = {3}, journal = {Journal of Laboratory and Precision Medicine}, issn = {2519-9005}, doi = {10.21037/jlpm.2018.04.02}, pages = {6}, language = {en} } @misc{SchneiderWeissRuheetal., author = {Schneider, Jens and Weiss, Romano and Ruhe, Madeleine and Jung, Tobias and Roggenbuck, Dirk and Stohwasser, Ralf and Schierack, Peter and R{\"o}diger, Stefan}, title = {Open source bioimage informatic tools for the analysis of DNA damage and associated biomarkers}, series = {Journal of Laboratory and Precision Medicine}, volume = {4}, journal = {Journal of Laboratory and Precision Medicine}, issn = {2519-9005}, doi = {10.21037/jlpm.2019.04.05}, language = {en} } @misc{RuheRabeJurischkaetal., author = {Ruhe, Madeleine and Rabe, Dominik and Jurischka, Christoph and Schr{\"o}der, Julia and Schierack, Peter and Deckert, P. Markus and R{\"o}diger, Stefan}, title = {Molecular biomarkers of DNA damage in diffuse large-cell lymphoma-a review}, series = {Journal of Laboratory and Precision Medicine}, volume = {4}, journal = {Journal of Laboratory and Precision Medicine}, issn = {2519-9005}, doi = {10.21037/jlpm.2019.01.01}, pages = {20}, language = {en} } @misc{UmairMohsinAlietal., author = {Umair, Muhammad and Mohsin, Mashkoor and Ali, Qasim and Qamar, Muhammad U. and Raza, Shahbaz and Ali, Aamir and Guenther, Sebastian and Schierack, Peter}, title = {Prevalence and Genetic Relatedness of Extended Spectrum-ß-Lactamase-Producing Escherichia coli Among Humans, Cattle, and Poultry in Pakistan}, series = {Microbial drug resistance}, volume = {25}, journal = {Microbial drug resistance}, number = {9}, issn = {1931-8448}, doi = {10.1089/mdr.2018.0450}, pages = {1374 -- 1381}, language = {en} } @misc{WajidAwanSaleemietal., author = {Wajid, Muhammad and Awan, Asad Bashir and Saleemi, Muhammad Kashif and Weinreich, J{\"o}rg and Schierack, Peter and Sarwar, Yasra and Ali, Aamir}, title = {Multiple Drug Resistance and Virulence Profiling of Salmonella enterica Serovars Typhimurium and Enteritidis from Poultry Farms of Faisalabad, Pakistan}, series = {Microbial Drug Resistance}, volume = {25}, journal = {Microbial Drug Resistance}, number = {1}, issn = {1931-8448}, doi = {10.1089/mdr.2018.0121}, pages = {133 -- 142}, language = {en} } @misc{AkhtarGhauriParveenetal., author = {Akhtar, Nasrin and Ghauri, M. A. and Parveen, Sana and Farooq, M. and Ali, Aamir and Schierack, Peter}, title = {Comparative Analysis of Draft Genome Sequence of Rhodococcus sp. Eu-32 with Other Rhodococcus Species for Its Taxonomie Status and Sulfur Metabolism Potential}, series = {Current microbiology}, volume = {76}, journal = {Current microbiology}, number = {10}, issn = {1432-0991}, doi = {10.1007/s00284-019-01737-1}, pages = {1207 -- 1214}, language = {en} } @misc{OloweAdefioyeAjayeobaetal., author = {Olowe, Olugbenga Adekunle and Adefioye, Olusolabomi J. and Ajayeoba, T. A. and Schiebel, Juliane and Weinreich, J{\"o}rg and Ali, Aamir and Burdukiewicz, Michał and R{\"o}diger, Stefan and Schierack, Peter}, title = {Phylogenetic grouping and biofilm formation of Multidrug Resistant Escherichia coli Isolates from Humans, Animals and Food products in South-west Nigeria}, series = {Scientific African}, volume = {6}, journal = {Scientific African}, issn = {2468-2276}, doi = {10.1016/j.sciaf.2019.e00158}, pages = {11}, language = {en} } @misc{HerrmannRoedigerSchmidtetal., author = {Herrmann, Anna and R{\"o}diger, Stefan and Schmidt, Carsten and Schierack, Peter and Schedler, Uwe}, title = {Spatial Separation of Microbeads into Detection Levels by a Bioorthogonal Porous Hydrogel for Size-Selective Analysis und Increased Multiplexicity}, series = {Analytical chemistry}, volume = {91}, journal = {Analytical chemistry}, number = {13}, issn = {1520-6882}, doi = {10.1021/acs.analchem.9b01586.}, pages = {8484 -- 8491}, language = {en} } @misc{AwanSchiebelBoehmetal., author = {Awan, Asad Bashir and Schiebel, Juliane and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Sarwar, Yasra and Schierack, Peter and Ali, Aamir}, title = {Association of biofilm formation and cytotoxic potential with multidrug resistance in clinical isolates of Pseudomonas aeruginosa}, series = {EXCLI journal}, volume = {18}, journal = {EXCLI journal}, issn = {1611-2156}, pages = {79 -- 90}, language = {en} } @misc{DeutschmannRoggenbuckSchierack, author = {Deutschmann, Claudia and Roggenbuck, Dirk and Schierack, Peter}, title = {The loss of tolerance to CHI3L1-A putative role in inflammatory bowel disease?}, series = {Clinical Immunology}, volume = {199}, journal = {Clinical Immunology}, issn = {1521-6616}, doi = {10.1016/j.clim.2018.12.005}, pages = {12 -- 17}, language = {en} } @misc{DinterBurdukiewiczSchieracketal., author = {Dinter, Franziska and Burdukiewicz, Michał and Schierack, Peter and Lehmann, Werner and Nestler, J{\"o}rg and Dame, Gregory and R{\"o}diger, Stefan}, title = {Simultaneous detection and quantification of DNA and protein biomarkers in spectrum of cardiovascular diseases in a microfluidic microbead chip}, series = {Analytical and Bioanalytical Chemistry}, volume = {411}, journal = {Analytical and Bioanalytical Chemistry}, number = {29}, issn = {1618-2650}, doi = {10.1007/s00216-019-02199-x}, pages = {7725 -- 7735}, language = {en} } @misc{DeutschmannSowaMurugaiyanetal., author = {Deutschmann, Claudia and Sowa, Mandy and Murugaiyan, Jayaseelan and Roessler, Uwe and R{\"o}ber, Nadja and Conrad, Karsten and Laass, Martin W. and Bogdanos, Dimitrios Petrou and Sipeki, Nora and Papp, Maria and R{\"o}diger, Stefan and Roggenbuck, Dirk and Schierack, Peter}, title = {Identification of Chitinase-3-Like Protein 1 as a Novel Neutrophil Antigenic Target in Crohn's Disease}, series = {Journal of Crohn's and Colitis}, volume = {13}, journal = {Journal of Crohn's and Colitis}, number = {7}, issn = {1876-4479}, doi = {10.1093/ecco-jcc/jjz012}, pages = {894 -- 904}, abstract = {Background and Aims There is an increasing incidence of inflammatory bowel disease [IBD]. Autoimmune responses are involved in the pathophysiology of IBD, but their underlying pathways and target antigens have not yet been fully elucidated. Methods Autoantigenic targets in IBD were identified after separation of whole cell proteins isolated from neutrophils using two-dimensional electrophoresis and matrix assisted laser desorption ionization - time of flight mass spectrometry-based protein identification of the spots that displayed Western blotting signals with anti-neutrophil cytoplasmic antibody-positive sera. The prevalence of IgG, IgA and secretory IgA [sIgA] to chitinase 3-like protein 1 [CHI3L1] was analysed by enzyme-linked immunosorbent assays using recombinant CHI3L1 in 110 patients with Crohn's disease [CD], 95 with ulcerative colitis [UC], 126 with coeliac disease [CeD] and 86 healthy controls [HCs]. Results The 18-glycosylhydrolase family member CHI3L1 was identified as a neutrophil autoantigenic target. CD patients displayed significantly higher levels of IgG to CHI3L1 than patients with UC and CeD (p < 0.0001, respectively). IgA and sIgA to CHI3L1 was significantly higher in CD than in UC, CeD and HCs [p < 0.0001, respectively]. IgA and sIgA to CHI3L1 demonstrated the highest prevalence in CD [25.5\%, 28/110; and 41.8\%\%, 46/110] compared to HCs [2.3\%, 2/86; and 4.7\%\%, 4/86; p = 0.0015 and p < 0.0001] and are associated with a more complicated progression of CD. Conclusion CHI3L1 is a novel neutrophil autoantigenic target in CD. IgA and sIgA to CHI3L1 may serve as novel markers for CD and may facilitate the serological diagnosis of IBD.}, language = {en} } @misc{SowaMurugaiyanConradetal., author = {Sowa, Mandy and Murugaiyan, Jayaseelan and Conrad, Karsten and Laass, Martin W. and Bogdanos, Dimitrios Petrou and Papp, Maria and R{\"o}diger, Stefan and Roggenbuck, Dirk and Schierack, Peter}, title = {A novel neutrophil autoantigenic target in inflammatory bowel disease}, language = {en} } @misc{JurischkaDinterEfimovaetal., author = {Jurischka, Christoph and Dinter, Franziska and Efimova, Anastasia and Weiss, Romano and Schiebel, Juliane and Schulz, Christian and Fayziev, Bekzodjon and Schierack, Peter and Fischer, Thomas and R{\"o}diger, Stefan}, title = {An explorative study of polymers for 3D printing of bioanalytical test systems}, series = {Clinical Hemorheology and Microcirculation}, volume = {75}, journal = {Clinical Hemorheology and Microcirculation}, number = {1}, issn = {1875-8622}, doi = {10.3233/CH-190713}, pages = {57 -- 84}, abstract = {Background: The 3D printing is relevant as a manufacturing technology of functional models for forensic, pharmaceutical and bioanalytical applications such as drug delivery systems, sample preparation and point-of-care tests. Objective: Melting behavior and autofluorescence of materials are decisive for optimal printing and applicability of the product which are influenced by varying unknown additives. Methods: We have produced devices for bioanalytical applications from commercially available thermoplastic polymers using a melt-layer process. We characterized them by differential scanning calorimetry, fluorescence spectroscopy and functional assays (DNA capture assay, model for cell adhesion, bacterial adhesion and biofilm formation test). Results: From 14 tested colored, transparent and black materials we found only deep black acrylonitrile-butadiene-styrene (ABS) and some black polylactic acid (PLA) useable for fluorescence-based assays, with low autofluorescence only in the short-wave range of 300-400 nm. PLA was suitable for standard bioanalytical purposes due to a glass transition temperature of approximately 60°C, resistance to common laboratory chemicals and easy print processing. For temperature-critical methods, such as hybridization reactions up to 90°C, ABS was better suited. Conclusions: Autofluorescence was not a disadvantage per se but can also be used as a reference signal in assays. The rapid development of individual protocols for sample processing and analysis required the availability of a material with consistent quality over time. For fluorescence-based assays, the use of commercial standard materials did not seem to meet this requirement.}, language = {en} } @misc{LopensKrawczykPappetal., author = {Lopens, Steffi and Krawczyk, Marcin and Papp, Maria and Milkiewicz, Piotr and Schierack, Peter and Liu, Yudong and Wunsch, Ewa and Conrad, Karsten and Roggenbuck, Dirk}, title = {The search for the Holy Grail: autoantigenic targets in primary sclerosing cholangitis asscociated with diesease phenotype an neoplasia}, series = {Autoimmunity Highlights}, volume = {11}, journal = {Autoimmunity Highlights}, issn = {2038-3274}, doi = {10.1186/s13317-020-00129-x}, pages = {14}, language = {en} } @misc{SchmidtSchierackGerberetal., author = {Schmidt, Carsten and Schierack, Peter and Gerber, Ulrike and Schr{\"o}der, Christian and Choi, Youngeun and Bald, Ilko and Lehmann, Werner and R{\"o}diger, Stefan}, title = {Streptavidin Homologues for Applications on Solid Surfaces at High Temperatures}, series = {Langmuir}, volume = 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