@misc{SowaGrossmannKnuetteretal., author = {Sowa, Mandy and Großmann, Kai and Kn{\"u}tter, Ilka and Hiemann, Rico and R{\"o}ber, Nadja and Anderer, Ursula and Csernok, Elena and Bogdanos, Dimitrios Petrou and Borghi, Maria Orietta and Meroni, Pier Luigi and Schierack, Peter and Reinhold, Dirk and Conrad, Karsten and Roggenbuck, Dirk}, title = {Simultaneous automated screening and confirmatory testing for vasculitis-specific ANCA}, series = {PLoS ONE}, volume = {9}, journal = {PLoS ONE}, number = {9}, issn = {1932-6203}, doi = {10.1371/journal.pone.0107743}, pages = {11}, language = {en} } @misc{WillitzkiHiemannPetersetal., author = {Willitzki, Annika and Hiemann, Rico and Peters, Vanessa and Sack, Ulrich and Schierack, Peter and R{\"o}diger, Stefan and Anderer, Ursula and Conrad, Karsten and Bogdanos, Dimitrios Petrou and Reinhold, Dirk and Roggenbuck, Dirk}, title = {New PlatformTechnology for Comprehensive Serological Diagnostics of Autoimmune Diseases}, series = {Clinical and Developmental Immunology}, journal = {Clinical and Developmental Immunology}, issn = {1740-2530}, doi = {10.1155/2012/284740}, pages = {1 -- 8}, language = {en} } @misc{KundzerVolkovaBogdanosetal., author = {Kundzer, Alena V. and Volkova, Margarita V. and Bogdanos, Dimitrios Petrou and R{\"o}diger, Stefan and Schierack, Peter and Generalov, I. and Nevinsky, G. A. and Roggenbuck, Dirk}, title = {Deoxyribonuclease activity of polyclonal IgGs: a putative serological marker in patients with spondyloarthritides}, series = {Immunologic Research}, volume = {56}, journal = {Immunologic Research}, number = {2-3}, issn = {0257-277X}, doi = {10.1007/s12026-013-8424-9}, pages = {457 -- 464}, abstract = {Antibodies executing catalytic activity are referred to as antibody enzymes or short "abzymes" and may have diagnostic relevance. Abzymes with deoxyribonuclease (DNase) activity have been demonstrated in patients with autoimmune and infectious diseases. Despite several reports on the occurrence of DNase abzymes in systemic autoimmune rheumatic diseases, conclusive data about DNase activity of antibodies in patients with spondyloarthritides (SpAs) are lacking. In recent cross-sectional studies evaluating levels of IgG DNase activity in patients with psoriatic arthritis (PsA), reactive arthritis (ReA), and ankylosing spondylitis (AS), DNase activity of IgG has been assessed by the rivanol clot method and confirmed by agarose gel electrophoresis. Remarkably, levels of IgG DNase activity were significantly higher in sera of SpA patients than those in control subjects. In patients with PsA, ReA, and AS, a positive correlation of DNase IgG activity with synovitis, disease activity, and stage of spondylitis was observed, respectively. Given the involvement of autoimmune reactions in cytolysis and connective tissue degradation in PsA, ReA, and to a lesser extent in AS, abzymes might have an impact on the pathophysiology of SpAs. Detection of IgG DNase activity in patients suffering from SpA represents an exciting new research field and may assist in the differential diagnosis of SpA.}, language = {en} } @misc{SchierackRoedigerKuhletal., author = {Schierack, Peter and R{\"o}diger, Stefan and Kuhl, Christoph and Hiemann, Rico and Roggenbuck, Dirk and Li, Ganwu and Weinreich, J{\"o}rg and Berger, Enrico and Nolan, Lisa K. and Nicholson, Bryon and R{\"o}mer, Antje and Fr{\"o}mmel, Ulrike and Wieler, Lothar H. and Schr{\"o}der, Christian}, title = {Porcine E. coli: Virulence-Associated Genes, Resistance Genes and Adhesion and Probiotic Activity Tested by a New Screening Method}, series = {PLoS One}, volume = {8}, journal = {PLoS One}, number = {4}, doi = {10.1371/journal.pone.0059242}, pages = {13}, abstract = {We established an automated screening method to characterize adhesion of Escherichia coli to intestinal porcine epithelial cells (IPEC-J2) and their probiotic activity against infection by enteropathogenic E. coli (EPEC). 104 intestinal E. coli isolates from domestic pigs were tested by PCR for the occurrence of virulence-associated genes, genes coding for resistances to antimicrobial agents and metals, and for phylogenetic origin by PCR. Adhesion rates and probiotic activity were examined for correlation with the presence of these genes. Finally, data were compared with those from 93 E. coli isolates from wild boars. Isolates from domestic pigs carried a broad variety of all tested genes and showed great diversity in gene patterns. Adhesions varied with a maximum of 18.3 or 24.2 mean bacteria adherence per epithelial cell after 2 or 6 hours respectively. Most isolates from domestic pigs and wild boars showed low adherence, with no correlation between adhesion/probiotic activity and E. coli genes or gene clusters. The gene sfa/foc, encoding for a subunit of F1C fimbriae did show a positive correlative association with adherence and probiotic activity; however E. coli isolates from wild boars with the sfa/foc gene showed less adhesion and probiotic activity than E. coli with the sfa/foc gene isolated from domestic pigs after 6 hour incubation. In conclusion, screening porcine E. coli for virulence associated genes genes, adhesion to intestinal epithelial cells, and probiotic activity revealed a single important adhesion factor, several probiotic candidates, and showed important differences between E. coli of domestic pigs and wild boars.}, language = {en} } @misc{RoggenbuckReinholdWerneretal., author = {Roggenbuck, Dirk and Reinhold, Dirk and Werner, Leal and Schierack, Peter and Bogdanos, Dimitrios Petrou and Conrad, Karsten}, title = {Glycoprotein 2 antibodies in Crohn's disease.}, series = {Advances in Clinical Chemistry}, volume = {60}, journal = {Advances in Clinical Chemistry}, issn = {0065-2423}, pages = {187 -- 208}, language = {en} } @misc{BednorzOelschlaegerKinnemannetal., author = {Bednorz, Carmen and Oelschlaeger, Kathrin and Kinnemann, Bianca and Hartmann, Susanne and Neumann, Konrad and Pieper, Robert and Bethe, Astrid and Semmler, Torsten and Tedin, Karsten and Schierack, Peter and Wieler, Lothar H. and Guenther, Sebastian}, title = {The broader context of antibiotic resistance: zinc feed supplementation of piglets increases the proportion of multi-resistant Escherichia coli in vivo}, series = {International Journal of Medical Microbiology}, volume = {303}, journal = {International Journal of Medical Microbiology}, number = {6-7}, issn = {1618-0607}, doi = {10.1016/j.ijmm.2013.06.004}, pages = {396 -- 403}, language = {en} } @misc{FroemmelLehmannRoedigeretal., author = {Fr{\"o}mmel, Ulrike and Lehmann, Werner and R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Weinreich, J{\"o}rg and Groß, Julia and Roggenbuck, Dirk and Zinke, Olaf and Ansorge, Hermann and Vogel, Steffen and Klemm, Per and Wex, Thomas and Schr{\"o}der, Christian and Wieler, Lothar H. and Schierack, Peter}, title = {Adhesion of human and animal Escherichia coli strains in association with their virulence-associated genes and phylogenetic origins}, series = {Applied and environmental microbiology}, volume = {79}, journal = {Applied and environmental microbiology}, number = {19}, issn = {1098-5336}, doi = {10.1128/AEM.01384-13}, pages = {5814 -- 5829}, abstract = {Intestinal colonization is influenced by the ability of the bacterium to inhabit a niche, which is based on the expression of colonization factors. Escherichia coli carries a broad range of virulence-associated genes (VAGs) which contribute to intestinal (inVAGs) and extraintestinal (exVAGs) infection. Moreover, initial evidence indicates that inVAGs and exVAGs support intestinal colonization. We developed new screening tools to genotypically and phenotypically characterize E. coli isolates originating in humans, domestic pigs, and 17 wild mammal and avian species. We analyzed 317 isolates for the occurrence of 44 VAGs using a novel multiplex PCR microbead assay (MPMA) and for adhesion to four epithelial cell lines using a new adhesion assay. We correlated data for the definition of new adhesion genes. inVAGs were identified only sporadically, particularly in roe deer (Capreolus capreolus) and the European hedgehog ( Erinaceus europaeus). The prevalence of exVAGs depended on isolation from a specific host. Human uropathogenic E. coli isolates carried exVAGs with the highest prevalence, followed by badger (Meles meles) and roe deer isolates. Adhesion was found to be very diverse. Adhesion was specific to cells, host, and tissue, though it was also unspecific. Occurrence of the following VAGs was associated with a higher rate of adhesion to one or more cell lines: afa-dra, daaD, tsh, vat, ibeA, fyuA, mat, sfa-foc, malX, pic, irp2, and papC. In summary, we established new screening methods which enabled us to characterize large numbers of E. coli isolates. We defined reservoirs for potential pathogenic E. coli. We also identified a very broad range of colonization strategies and defined potential new adhesion genes.}, language = {en} } @misc{RoggenbuckHiemannSchieracketal., author = {Roggenbuck, Dirk and Hiemann, Rico and Schierack, Peter and Reinhold, Dirk and Conrad, Karsten}, title = {Digital immunofluorescence enables automated detection of antinuclear antibody endpoint titers avoiding serial dilution}, series = {Clinical Chemistry and Laboratory Medicine}, volume = {52}, journal = {Clinical Chemistry and Laboratory Medicine}, number = {2}, issn = {1437-4331}, doi = {10.1515/cclm-2013-0543}, pages = {e9 -- e11}, language = {en} } @misc{WillitzkiLorenzHiemannetal., author = {Willitzki, Annika and Lorenz, Sebastian and Hiemann, Rico and Guttek, Karina and Goihl, Alexander and Hartig, Roland and Conrad, Karsten and Feist, Eugen and Sack, Ulrich and Schierack, Peter and Heiserich, Lisa and Eberle, Caroline and Peters, Vanessa and Roggenbuck, Dirk and Reinhold, Dirk}, title = {Fully automated analysis of chemically induced γH2AX foci in human peripheral blood mononuclear cells by indirect immunofluorescence}, series = {Cytometry Part A}, volume = {83}, journal = {Cytometry Part A}, number = {11}, issn = {1552-4930}, doi = {10.1002/cyto.a.22350}, pages = {1017 -- 1026}, language = {en} } @misc{BednorzGuentherOelschlaegeretal., author = {Bednorz, Carmen and Guenther, Sebastian and Oelschlaeger, Kathrin and Pieper, Robert and Hartmann, Susanne and Tedin, Karsten and Semmler, Torsten and Neumann, Konrad and Schierack, Peter and Bethe, Astrid and Wieler, Lothar H. and Kinnemann, Bianca}, title = {Feeding the Probiotic Enterococcus faecium Strain NCIMB 10415 to Piglets Specifically Reduces the Number of Escherichia coli Pathotypes That Adhere to the Gut Mucosa}, series = {Applied and Environmental Microbiology}, volume = {79}, journal = {Applied and Environmental Microbiology}, number = {24}, issn = {0099-2240}, doi = {10.1128/AEM.03138-13}, pages = {7896 -- 7904}, language = {en} } @misc{FroemmelBoehmNitschkeetal., author = {Fr{\"o}mmel, Ulrike and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Weinreich, J{\"o}rg and Groß, Julia and R{\"o}diger, Stefan and Wex, Thomas and Ansorge, Hermann and Zinke, Olaf and Schr{\"o}der, Christian and Roggenbuck, Dirk and Schierack, Peter}, title = {Adhesion patterns of commensal and pathogenic Escherichia coli from humans and wild animals on human and porcine epithelial cell lines}, series = {Gut Pathogens}, volume = {5}, journal = {Gut Pathogens}, number = {31}, issn = {1757-4749}, doi = {10.1186/1757-4749-5-31}, pages = {8}, abstract = {Abstract BACKGROUND: Different strategies of colonization or infection by E. coli result in formation of certain adhesion patterns which help also in classifying intestinal E. coli into pathotypes. Little is known about adhesion patterns and host- and tissue adaption of commensal E. coli and about E. coli originating in clinically healthy hosts carrying pathotype-specific virulence-associated genes. FINDINGS: Adhesion pattern of E. coli (n = 282) from humans and from 18 animal species were verified on intestinal human Caco-2 and porcine IPEC-J2 cells and, furthermore, for comparison on human urinary bladder 5637, porcine kidney PK-15 epithelial and HEp-2 cells. The analysis was carried out on 150,000 images of adhesion assays.Adhesion patterns were very diverse; 88 isolates were completely non-adherent, whereas 194 adhered to at least one cell line with the dominant adhesion patterns "diffusely distributed" and "microcolony formation". Adhesion patterns "chains" and "clumps" were also visible. Chain formation was mediated by the presence of epithelial cells. Clump formation was very specific on only the 5637 cell line. All enteropathogenic (eae+) E. coli (EPEC; n = 14) were able to form microcolonies which was cell line specific for each isolate. Most EPEC formed microcolonies on intestinal IPEC-J2 and Caco-2 but several also on urinary tract cells. Shigatoxin-producing (stx+) E. coli (n = 10) showed no specific adhesion patterns. CONCLUSIONS: E. coli isolates were highly diverse. Commensal and pathogenic isolates can adhere in various forms, including diffuse distribution, microcolonies, chains and clumps. Microcolony formation seems to be a global adhesion strategy also for commensal E. coli.}, language = {en} } @misc{GrossmannRoggenbuckSchroederetal., author = {Großmann, Kai and Roggenbuck, Dirk and Schr{\"o}der, Christian and Conrad, Karsten and Schierack, Peter and Sack, Ulrich}, title = {Multiplex assessment of non-organ-specific autoantibodies with a novel microbead-based immunoassay}, series = {Cytometry Part A}, volume = {79}, journal = {Cytometry Part A}, number = {2}, issn = {1552-4922}, doi = {10.1002/cyto.a.21009}, pages = {118 -- 125}, language = {en} } @misc{SchierackKletaTedinetal., author = {Schierack, Peter and Kleta, Sylvia and Tedin, Karsten and Babila, T. B. and Oswald, S. and Oelschlaeger, T. A. and Hiemann, Rico and Paetzold, S. and Wieler, Lothar H.}, title = {E. coli Nissle 1917 affects Salmonella adhesion to porcine intestinal epithelial cells}, series = {PLoS One}, volume = {6}, journal = {PLoS One}, number = {2}, doi = {10.1371/journal.pone.0014712}, pages = {10}, language = {en} } @misc{RoedigerRuhlandSchroederetal., author = {R{\"o}diger, Stefan and Ruhland, Mirko and Schr{\"o}der, Christian and Großmann, Kai and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Berger, Ingo and Schimke, Ingolf and Schierack, Peter}, title = {Fluorescence dye adsorption assay to quantify carboxyl groups on the surface of poly(methyl methacrylate) microbeads}, series = {Analytical Chemistry}, volume = {83}, journal = {Analytical Chemistry}, number = {9}, issn = {0003-2700}, doi = {10.1021/ac103277s}, pages = {3379 -- 3385}, language = {en} } @misc{SchierackWeinreichEwersetal., author = {Schierack, Peter and Weinreich, J{\"o}rg and Ewers, Christa and Tachu, Babila and Nicholson, Bryon and Barth, Stefanie}, title = {Hemolytic porcine intestinal E. coli without virulence-associated genes typical for intestinal pathogenic E. coli}, series = {Applied and environmental microbiology}, volume = {77}, journal = {Applied and environmental microbiology}, number = {23}, issn = {1098-5336}, doi = {10.1128/AEM.05289-11}, pages = {8451 -- 8455}, language = {en} } @misc{RoemerWielerSchierack, author = {R{\"o}mer, Antje and Wieler, Lothar H. and Schierack, Peter}, title = {Analyses of intestinal commensal Escherichia coli strains from wild boars suggest adaptation to conventional pig production conditions}, series = {Veterinary Microbiology}, volume = {16}, journal = {Veterinary Microbiology}, number = {1-2}, issn = {0378-1135}, doi = {10.1016/j.vetmic.2012.07.009}, pages = {122 -- 129}, language = {en} } @incollection{RoedigerSchierackBoehmetal., author = {R{\"o}diger, Stefan and Schierack, Peter and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Berger, Ingo and Fr{\"o}mmel, Ulrike and Schmidt, Carsten and Ruhland, Mirko and Schimke, Ingolf and Roggenbuck, Dirk and Lehmann, Werner and Schr{\"o}der, Christian}, title = {A highly versatile microscope imaging technology platform for the multiplex real-time detection of biomolecules and autoimmune antibodies}, series = {Molecular diagnostics}, booktitle = {Molecular diagnostics}, editor = {Seitz, Harald and Berger, Ingo}, publisher = {Springer}, address = {Berlin [u.a.]}, isbn = {978-3-642-37690-0}, pages = {35 -- 74}, language = {en} } @misc{RoggenbuckGoihlHanacketal., author = {Roggenbuck, Dirk and Goihl, Alexander and Hanack, Katja and Holzl{\"o}hner, Pamela and Hentschel, Christian and Veiczi, Miklos and Schierack, Peter and Reinhold, Dirk and Schulz, Hans-Ulrich}, title = {Serological diagnosis and prognosis of severe acute pancreatitis by analysis of serum glycoprotein 2}, series = {Clinical Chemistry and Laboratory Medicine}, volume = {55}, journal = {Clinical Chemistry and Laboratory Medicine}, number = {6}, issn = {1437-4331}, doi = {10.1515/cclm-2016-0797}, pages = {854 -- 864}, language = {en} } @misc{DurkalecKolendaOwczareketal., author = {Durkalec, Maciej and Kolenda, Rafał and Owczarek, Tomasz and Szkoda, J{\´o}zef and Nawrocka, Agnieszka and Grzegrzolka, Jędrzej and Dziegiel, Piotr and Socha, Piotr and Kolacz, Roman and Schierack, Peter and Zmudzki, Jan and Posyniak, Andrzej}, title = {Expression of metallothionein in the liver and kidneys of the red deer (Cervus elaphus L.) from an industrial metal smelting area of Poland}, series = {Ecotoxicology and Environmental Safety}, volume = {137}, journal = {Ecotoxicology and Environmental Safety}, issn = {1090-2414}, doi = {10.1016/j.ecoenv.2016.11.018}, pages = {121 -- 129}, language = {en} } @misc{GrzymajloUgorskiSuchanskietal., author = {Grzymajlo, Krzysztof and Ugorski, Maciej and Suchanski, Jaroslaw and Kedzierska, Anna E. and Kolenda, Rafał and Jarzab, Anna and Biernatowska, Agnieszka and Schierack, Peter}, title = {The Novel Type 1 Fimbriae FimH Receptor Calreticulin Plays a Role in Salmonella Host Specificity}, series = {Frontiers in Cellular and Infection Microbiology}, volume = {7}, journal = {Frontiers in Cellular and Infection Microbiology}, issn = {2235-2988}, doi = {10.3389/fcimb.2017.00326}, pages = {15}, language = {en} } @misc{MohsinRazaSchaufleretal., author = {Mohsin, Mashkoor and Raza, Shahbaz and Schaufler, Katharina and Roschanski, Nicole and Sarwar, Fatima and Semmler, Torsten and Schierack, Peter and Guenther, Sebastian}, title = {High Prevalence of CTX-M-15-Type ESBL-Producing E. coli from Migratory Avian Species in Pakistan}, series = {Frontiers in microbiology}, volume = {8}, journal = {Frontiers in microbiology}, number = {2476}, issn = {1664-302X}, doi = {10.3389/fmicb.2017.02476}, pages = {9}, language = {en} } @misc{ZhangLuoWuetal., author = {Zhang, Shulan and Luo, Jing and Wu, Ziyan and Roggenbuck, Dirk and Schierack, Peter and Reinhold, Dirk and Li, Ji and Zeng, Xiaofeng and Zhang, Fengchun and Qian, Jiaming and Li, Yongzhe}, title = {Antibodies against glycoprotein 2 display diagnostic advantages over ASCA in distinguishing CD from intestinal tuberculosis and intestinal Beh{\c{c}}et's disease}, series = {Clinical and Translational Gastroenterology}, volume = {9}, journal = {Clinical and Translational Gastroenterology}, number = {2}, issn = {2155-384X}, doi = {10.1038/ctg.2018.1}, pages = {133}, language = {en} }