@misc{SydowEgerSchwabeetal., author = {Sydow, Katharina and Eger, Elias and Schwabe, Michael and Heiden, Stefan E. and Bohnert, J{\"u}rgen A. and Franzenburg, S{\"o}ren and Jurischka, Christoph and Schierack, Peter and Schaufler, Katharina}, title = {Geno- and Phenotypic Characteristics of a Klebsiella pneumoniae ST20 Isolate with Unusual Colony Morphology}, series = {Microorganisms}, volume = {10}, journal = {Microorganisms}, number = {10}, issn = {2076-2607}, doi = {10.3390/microorganisms10102063}, abstract = {Klebsiella pneumoniae is a common member of the intestinal flora of vertebrates. In addition to opportunistic representatives, hypervirulent (hvKp) and antibiotic-resistant K. pneumoniae (ABR-Kp) occur. While ABR-Kp isolates often cause difficult-to-treat diseases due to limited therapeutic options, hvKp is a pathotype that can infect healthy individuals often leading to recurrent infection. Here, we investigated the clinical K. pneumoniae isolate PBIO3459 obtained from a blood sample, which showed an unusual colony morphology. By combining whole-genome and RNA sequencing with multiple in vitro and in vivo virulence-associated assays, we aimed to define the respective Klebsiella subtype and explore the unusual phenotypic appearance. We demonstrate that PBIO3459 belongs to sequence type (ST)20 and carries no acquired resistance genes, consistent with phenotypic susceptibility tests. In addition, the isolate showed low-level virulence, both at genetic and phenotypic levels. We thus suggest that PBIO3459 is an opportunistic (commensal) K. pneumoniae isolate. Genomic comparison of PBIO3459 with closely related ABR-Kp ST20 isolates revealed that they differed only in resistance genes. Finally, the unusual colony morphology was mainly associated with carbohydrate and amino acid transport and metabolism. In conclusion, our study reveals the characteristics of a Klebsiella sepsis isolate and suggests that opportunistic representatives likely acquire and accumulate antibiotic resistances that subsequently enable their emergence as ABR-Kp pathogens.}, language = {en} } @misc{RanjanScholzSemmleretal., author = {Ranjan, Amit and Scholz, Julia and Semmler, Torsten and Wieler, Lothar H. and Ewers, Christa and M{\"u}ller, Stefanie and Pickard, Derek J. and Schierack, Peter and Tedin, Karsten and Ahmed, Niyaz and Schaufler, Katharina and Guenther, Sebastian}, title = {ESBL-plasmid carriage in E. coli enhances in vitro bacterial competition fitness and serum resistance in some strains of pandemic sequence types without overall fitness cost}, series = {Gut pathogens}, volume = {10}, journal = {Gut pathogens}, number = {24}, issn = {1757-4749}, doi = {10.1186/s13099-018-0243-z}, pages = {9}, language = {en} } @misc{KhanKolendaSchieracketal., author = {Khan, Muhammad Moman and Kolenda, Rafał and Schierack, Peter and Weinreich, J{\"o}rg and R{\"o}diger, Stefan and Schierack, Jakob and Stubbe, Michael and Lkhagvasuren, Davaa and Guenther, Sebastian and Schaufler, Katharina}, title = {Investigation of Commensal Escherichia coli Populations of Cormorant Hatchlings in the Absence of Anthropogenic Impacts in Remote Areas of West Mongolia}, series = {Microorganisms}, volume = {9}, journal = {Microorganisms}, number = {2}, issn = {2076-2607}, doi = {10.3390/microorganisms9020372}, language = {en} } @misc{MohsinRazaSchaufleretal., author = {Mohsin, Mashkoor and Raza, Shahbaz and Schaufler, Katharina and Roschanski, Nicole and Sarwar, Fatima and Semmler, Torsten and Schierack, Peter and Guenther, Sebastian}, title = {High Prevalence of CTX-M-15-Type ESBL-Producing E. coli from Migratory Avian Species in Pakistan}, series = {Frontiers in microbiology}, volume = {8}, journal = {Frontiers in microbiology}, number = {2476}, issn = {1664-302X}, doi = {10.3389/fmicb.2017.02476}, pages = {9}, language = {en} } @misc{SchierackHeidenKhanetal., author = {Schierack, Peter and Heiden, Stefan E. and Khan, Muhammad Moman and Nikolaus, Lena and Kolenda, Rafał and Stubbe, Michael and Lkhagvasuren, Davaa and R{\"o}diger, Stefan and Guenther, Sebastian and Schaufler, Katharina}, title = {Genomic and Phenotypic Analysis of an ESBL-Producing E. coli ST1159 Clonal Lineage From Wild Birds in Mongolia}, series = {Frontiers in Microbiology}, volume = {11}, journal = {Frontiers in Microbiology}, issn = {1664-302X}, doi = {10.3389/fmicb.2020.01699}, pages = {7}, language = {en} } @misc{RoedigerKramerFroemmeletal., author = {R{\"o}diger, Stefan and Kramer, Toni and Fr{\"o}mmel, Ulrike and Weinreich, J{\"o}rg and Roggenbuck, Dirk and Guenther, Sebastian and Schaufler, Katharina and Schr{\"o}der, Christian and Schierack, Peter}, title = {Intestinal Escherichia coli colonization in a mallard duck population over four consecutive winter season}, series = {Environmental Microbiology}, volume = {17}, journal = {Environmental Microbiology}, number = {9}, issn = {1462-2920}, doi = {10.1111/1462-2920.12807}, pages = {3352 -- 3361}, language = {en} }