@misc{SpitzerRichterKnautetal., author = {Spitzer, Stefan G. and Richter, P. and Knaut, M. and Sch{\"u}ler, S.}, title = {Treatment of Atrial Fibrillation in Open Heart Surgery. The Potential Role of Microwave Energy}, series = {The Thoracic and Cardiovascular Surgeon}, volume = {47}, journal = {The Thoracic and Cardiovascular Surgeon}, number = {Supplement 3}, issn = {0946-4778}, pages = {374 -- 378}, language = {en} } @misc{KnautSpitzerKarolyietal., author = {Knaut, M. and Spitzer, Stefan G. and Karolyi, L. and Ebert, H.-H. and Richter, P. and Tugtekin, Sems M. and Schuler, S.}, title = {Intraoperative Microwave Ablation for Curative Treatment of Atrial Fibrillation in Open Heart Surgery - The MICRO-STAF and MICRO-PASS Pilot Trial}, series = {The thoracic and cardiovascular surgeon}, volume = {47}, journal = {The thoracic and cardiovascular surgeon}, number = {Suppl. 3}, issn = {1439-1902}, doi = {10.1055/s-2007-1013205}, pages = {379 -- 384}, abstract = {Concomitant microwave ablation for curative treatment of atrial fibrillation (AF) was performed in 18 patients with history of chronic atrial fibrillation and indication for open heart surgery, 11 patients with mitral valve replacement and 7 patients with coronary artery bypass grafting. There were no perioperative complications. During the postoperative period most of the patients had intermittent AF, they received low dose Sotalol therapy and electric cardio-versions. Up to now seven patients have reached follow-up day 90. One patient has persistent AF. Two patients had typical atrial flutter that was electrically converted to sinus rhythm (SR), isthmus ablation is planned. The other four patients have SR, one patient without cardioversions. These four patients show recovered atrial function with observed A-wave for transmitral flow. Under visual guidance the continuous atrial lesion lines could be induced effectively and safely by the intraoperative device Lynx.}, language = {en} } @misc{HenzeHuettnerKochetal., author = {Henze, Henriette and H{\"u}ttner, S{\"o}ren S. and Koch, Philipp and Sch{\"u}ler, Svenja C. and Groth, Marco and von Eyss, Bj{\"o}rn and von Maltzahn, Julia}, title = {Denervation alters the secretome of myofibers and thereby affects muscle stem cell lineage progression and functionality}, series = {npj regenerative medicine}, volume = {9}, journal = {npj regenerative medicine}, number = {1}, publisher = {Nature Publishing Group UK}, address = {London}, issn = {2057-3995}, doi = {10.1038/s41536-024-00353-3}, pages = {1 -- 14}, abstract = {Skeletal muscle function crucially depends on innervation while repair of skeletal muscle relies on resident muscle stem cells (MuSCs). However, it is poorly understood how innervation affects MuSC properties and thereby regeneration of skeletal muscle. Here, we report that loss of innervation causes precocious activation of MuSCs concomitant with the expression of markers of myogenic differentiation. This aberrant activation of MuSCs after loss of innervation is accompanied by profound alterations on the mRNA and protein level. Combination of muscle injury with loss of innervation results in impaired regeneration of skeletal muscle including shifts in myogenic populations concomitant with delayed maturation of regenerating myofibers. We further demonstrate that loss of innervation leads to alterations in myofibers and their secretome, which then affect MuSC behavior. In particular, we identify an increased secretion of Osteopontin and transforming growth factor beta 1 (Tgfb1) by myofibers isolated from mice which had undergone sciatic nerve transection. The altered secretome results in the upregulation of early activating transcription factors, such as Junb , and their target genes in MuSCs. However, the combination of different secreted factors from myofibers after loss of innervation is required to cause the alterations observed in MuSCs after loss of innervation. These data demonstrate that loss of innervation first affects myofibers causing alterations in their secretome which then affect MuSCs underscoring the importance of proper innervation for MuSC functionality and regeneration of skeletal muscle.}, language = {en} }