@misc{MartinLehmannSacketal., author = {Martin, Frank and Lehmann, Mario and Sack, Ulrich and Anderer, Ursula}, title = {In vitro development of personalized cartilage microtissues uncovers an individualized differentiation capacity of human chondrocytes}, series = {Experimental Biology and Medicine}, volume = {242}, journal = {Experimental Biology and Medicine}, number = {18}, issn = {1535-3702}, doi = {10.1177/1535370217728498}, pages = {1746 -- 1756}, language = {en} } @misc{LehmannMartinMannigeletal., author = {Lehmann, Mario and Martin, Frank and Mannigel, Kerstin and Kaltschmidt, Kristin and Sack, Ulrich and Anderer, Ursula}, title = {Three-dimensional scaffold-free fusion culture: the way to enhanced chondrogenesis of in vitro propagated human articular chondrocytes}, series = {European Journal of Histochemistry}, volume = {57}, journal = {European Journal of Histochemistry}, number = {4}, issn = {2038-8306}, doi = {10.4081/ejh.2013.e31}, pages = {205 -- 215}, language = {en} } @misc{MartinLehmannSchlaegeretal., author = {Martin, Frank and Lehmann, Mario and Schl{\"a}ger, Peter and Sack, Ulrich and Anderer, Ursula}, title = {Differentiation capacity of chondrocytes in microtissues depends on TGF-ß subtyp}, series = {Journal of biochips \& tissue chips : JBTC}, volume = {S2}, journal = {Journal of biochips \& tissue chips : JBTC}, number = {002}, issn = {2153-0777}, doi = {10.4172/2153-0777.S2-002}, pages = {11}, language = {en} } @misc{WillitzkiHiemannPetersetal., author = {Willitzki, Annika and Hiemann, Rico and Peters, Vanessa and Sack, Ulrich and Schierack, Peter and R{\"o}diger, Stefan and Anderer, Ursula and Conrad, Karsten and Bogdanos, Dimitrios Petrou and Reinhold, Dirk and Roggenbuck, Dirk}, title = {New PlatformTechnology for Comprehensive Serological Diagnostics of Autoimmune Diseases}, series = {Clinical and Developmental Immunology}, journal = {Clinical and Developmental Immunology}, issn = {1740-2530}, doi = {10.1155/2012/284740}, pages = {1 -- 8}, language = {en} } @inproceedings{LehmannMartinScholkaetal., author = {Lehmann, Mario and Martin, Frank and Scholka, Jenny and Petto, Carola and Wittenburg, Gretel and Sack, Ulrich and Anderer, Ursula}, title = {Human chondrocytes and dental pulp stem cells enhance their chondrogenic differentiation potential via a two-step fusion culture technique}, series = {Tissue Engineering, Part A, Vol. 21, Suppl. 1}, booktitle = {Tissue Engineering, Part A, Vol. 21, Suppl. 1}, doi = {10.1089/ten.tea.2015.5000.abstracts}, pages = {S-268 -- S-269}, language = {en} } @misc{HiemannHilgerMicheletal., author = {Hiemann, Rico and Hilger, Nadja and Michel, J{\"o}rg and Nitschke, J{\"o}rg and B{\"o}hm, Alexander and Anderer, Ursula and Weigert, Martin and Sack, Ulrich}, title = {Automatic Analysis of Immunofluorescence Patterns of HEp-2 Cells}, series = {Annals of the New York Academy of Sciences}, volume = {1109}, journal = {Annals of the New York Academy of Sciences}, number = {1}, issn = {0077-8923}, doi = {10.1196/annals.1398.042}, pages = {358 -- 371}, abstract = {The standard screening test for the recognition of autoimmune diseases is the proof of autoantibodies in serum of patients by indirect immunofluorescence (IIF) based on HEp-2 cells. Manual evaluation of this test is very subjective, slow, and there are no objective parameters as guidelines available. Interlaboratory tests showed occasionally large deviations in the test evaluation resulting in a high variance of results. The aim of this project is fast, objective, safe, and economical automatic analysis of HEp-2 IIF patterns. Images of IIF patterns were completely and automatically captured using an inverse motorized fluorescence microscope. Thereby, device-specific parameters were controlled automatically, too. For fast analysis of IIF patterns new algorithms of image processing were developed. Artifacts were recognized and excluded from analysis by the developed software. Analysis of more than 80,000 images clearly demonstrated full automatization and fast processing of IIF patterns. Additionally serum-specific fluorescence could be easily distinguished from background. Even very weak but positive patterns can be recognized and used for diagnosis. A detailed separation into different basic patterns is possible. Objective, fast, and disease-related economical analysis of HEp-2 immunofluorescence patterns is feasible. The implemented software algorithms allowed a mathematical way of describing IIF patterns and can therefore be a useful tool for the needed standardization process.}, language = {en} } @misc{HiemannRoggenbuckSacketal., author = {Hiemann, Rico and Roggenbuck, Dirk and Sack, Ulrich and Anderer, Ursula and Conrad, Karsten}, title = {Die HEp-2-Zelle als Target f{\"u}r multiparametrische Autoantik{\"o}rperanalytik - Automatisierung und Standardisierung/The HEp-2 cell as target for multiparametric autoantibody analyses: automation and standardisation}, series = {Journal of Laboratory Medicine}, volume = {35}, journal = {Journal of Laboratory Medicine}, number = {6}, issn = {1439-0477}, doi = {10.1515/JLM.2011.053}, pages = {351 -- 361}, abstract = {Trotz Einf{\"u}hrung von Multiplex-Assays ist die Bestimmung von Autoantik{\"o}rpern (AAK) mittels indirekter Immunfluoreszenz (IIF) nach wie vor ein wesentlicher Bestandteil der Autoimmundiagnostik. Das Screening auf nichtorganspezifische AAK wird in der Routinediagnostik von autoimmunen rheumatischen Erkrankungen mittels indirekter Immunfluoreszenz an HEp-2-Zellen durchgef{\"u}hrt. Leider differieren die mit diesem Test erhaltenen Befunde z. T. recht erheblich auf Grund von objektiven (z. B. HEp-2-Zellfixierung) und subjektiven (z. B. Erfahrungswerte) Faktoren. Die Intra- und Interlaborvarianzen sind daher relativ hoch. Erste Ergebnisse von Evaluierungsstudien eines neu entwickelten Systems zur automatischen Bildanalyse (AKLIDES®) zeigen einen Weg in Richtung Standardisierung (bzw. Harmonisierung) des HEp-2-Zell-Assays auf, in dem zun{\"a}chst subjektive Fehlinterpretationen vermieden werden k{\"o}nnen. Eine weitere Harmonisierung erfordert die Weiterentwicklung der Software-Algorithmen zur Mustererkennung sowie neue Kalibrationssysteme.}, language = {en} }