@misc{KuhnBenndorfRappetal., author = {Kuhn, Ramona and Benndorf, Dirk and Rapp, Erdmann and Reichl, Udo and Palese, Luigi Leonardo and Pollice, Alfieri}, title = {Metaproteome analysis of sewage sludge from membrane bioreactors}, series = {Proteomics}, volume = {11}, journal = {Proteomics}, number = {13}, issn = {1615-9861}, doi = {10.1002/pmic.201000590}, pages = {2738 -- 2744}, abstract = {Microbial dynamics and enzymatic activities of activated sludge processes are not completely understood yet. A better understanding about the biology is indispensable for further process optimization. Since proteins play a key role as catalysts in sludge processes, a protocol for protein extraction and analysis by 2-D PAGE was established. It is based on phenol extraction of alkaline extracts and on a subsequent precipitation with ammonium sulphate. 2-D protein patterns obtained from different sludges collected from membrane bioreactors showed - besides common spots - significant differences. Selected proteins were identified with nano- HPLC-ESI-MS/MS. All membrane biological reactor (MBR) sludge samples investigated in this study contained elastase 3A, which implies that this human serine protease is a significant constituent of municipal wastewater. Although the identification of proteins from ammonia-oxidizing bacterium Nitrosomonas europaea was expected, the detection of a protein with homology to the marine bacterium Saprospira grandis in MBR1 was surprising.}, language = {en} }