@misc{HuebenerHoffmannKeuleretal., author = {H{\"u}bener, Heike and Hoffmann, Peter and Keuler, Klaus and Pfeifer, Susanne and Ramthun, Hans and Spekat, Arne and Steger, Christian and Warrach-Sagi, Kirsten}, title = {Deriving user-informed climate information from climate model ensemble results}, series = {Advances in Science and Research}, volume = {14}, journal = {Advances in Science and Research}, issn = {1992-0628}, doi = {10.5194/asr-14-261-2017}, pages = {261 -- 269}, language = {en} } @misc{SteinbrechtPfeiferHerzogetal., author = {Steinbrecht, Susanne and Pfeifer, Nadine and Herzog, Natalie and Katzenberger, Nadine and Schulz, Christian and Kammerer, Sarah and K{\"u}pper, Jan-Heiner}, title = {HepG2-1A2 C2 and C7: Lentivirus vector-mediated stable and functional overexpression of cytochrome P450 1A2 in human hepatoblastoma cells}, series = {Toxicology Letters}, volume = {319}, journal = {Toxicology Letters}, issn = {0378-4274}, doi = {10.1016/j.toxlet.2019.11.006}, pages = {155 -- 159}, abstract = {Novel HepG2 cell clones 1A2 C2 and 1A2 C7 were independently generated by lentiviral transduction to functionally overexpress cytochrome P450 1A2 (CYP1A2). We found similar and stable CYP1A2 transcript and protein levels in both cell clones leading to specific enzyme activities of about 370 pmol paracetamol x min-1 x mg-1 protein analyzed by phenacetin conversion. Both clones showed dramatically increased sensitivity to the hepatotoxic compound aflatoxin B1 (EC50<100 nM) when compared to parental HepG2 cells (EC50 ∼5 μM). Thus, newly established cell lines are an appropriate tool to study metabolism and toxicity of substances depending on conversion by CYP1A2.}, language = {en} } @misc{BuelowHuebenerKeuleretal., author = {B{\"u}low, Katharina and H{\"u}bener, Heike and Keuler, Klaus and Menz, Christoph and Pfeifer, Susanne and Ramthun, Hans and Spekat, Arne and Steger, Christian and Teichmann, Claas and Warrach-Sagi, Kirsten}, title = {User tailored results of a regional climate model ensemble to plan adaption to the changing climate in Germany}, series = {Advances in Science and Research}, volume = {16}, journal = {Advances in Science and Research}, issn = {1992-0636}, doi = {10.5194/asr-16-241-2019}, pages = {241 -- 249}, abstract = {In the German regional climate modeling project ReKliEs-De the existing EURO-CORDEX simulations have been systematically complemented by new simulations to derive more robust ranges of possible future climate change. The focus of the project lay on user tailored results, which are required for the planning of measures to adapt to the changing climate. Changes in temperature and precipitation indices are calculated from a multi model ensemble for the end of the 21st century. The results for the mitigation scenario RCP2.6 are compared to the results of the "business as usual" scenario RCP8.5. Averaged over Germany the increase of mean annual temperature and of the number of summer days will be around 3 times higher for RCP8.5 than for RCP2.6. In summer, the increase of dry days could be twice as high in RCP8.5 compared to RCP2.6.}, language = {en} }