@misc{DeutschmannDinterRoedigeretal., author = {Deutschmann, Claudia and Dinter, Franziska and R{\"o}diger, Stefan and Roggenbuck, Dirk and Schierack, Peter}, title = {Comparison of Lab and Point of Care (POC) technologies - case study for CHI3L1, Potsdam Days on Bioanalysis}, pages = {1}, language = {en} } @inproceedings{RoedigerBoehmNitschkeetal., author = {R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Stavitskaya, Luba and Gruner, Melanie and Kundzer, Alena V. and Volkova, Margarita V. and Generalov, I. and Schmidt, Carsten and Schr{\"o}der, Christian and Roggenbuck, Dirk and Schierack, Peter}, title = {Development of a Method for Multiplex Real-Time Analysis of Enzymati c Activity on a Microbead-Chip.}, series = {Infections, Tumors and Autoimmunity, Report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 01. - 04., 2013}, booktitle = {Infections, Tumors and Autoimmunity, Report on the 11th Dresden Symposium on Autoantibodies held in Dresden on September 01. - 04., 2013}, publisher = {Pabst Science Publ.}, address = {Lengerich}, isbn = {978-3-89967-881-9}, pages = {280}, language = {en} } @inproceedings{RoedigerHanschmannSteidleetal., author = {R{\"o}diger, Stefan and Hanschmann, Henning and Steidle, Michael and Schr{\"o}der, Christian and Schierack, Peter and Lehmann, Werner}, title = {Detection of Borrelia, Bordetella and HPV with the LoopTag Real-Time PCR Probe System}, series = {International journal of medical microbiology}, volume = {303}, booktitle = {International journal of medical microbiology}, number = {Supplement 1}, issn = {1618-0607}, pages = {12 -- 13}, language = {en} } @misc{DinterDeutschmannSchieracketal., author = {Dinter, Franziska and Deutschmann, Claudia and Schierack, Peter and Dame, Gregory and R{\"o}diger, Stefan}, title = {Integration of Cardiovascular Disease Biomarkers in a Microfluidic Microbead Chip}, pages = {1}, language = {en} } @misc{SchiebelBoehmNitschkeetal., author = {Schiebel, Juliane and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Burdukiewicz, Michał and Weinreich, J{\"o}rg and Ali, Aamir and Roggenbuck, Dirk and R{\"o}diger, Stefan and Schierack, Peter}, title = {Genotypic and phenotypic characteristics in association with biofilm formation in different pathotypes of human clinical Escherichia coli isolates}, series = {Applied and Environmental Microbiology}, volume = {83}, journal = {Applied and Environmental Microbiology}, number = {24}, issn = {1098-5336}, doi = {10.1128/AEM.01660-17}, language = {en} } @misc{LiebschRoedigerBoehmetal., author = {Liebsch, Claudia and R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Weinreich, J{\"o}rg and Fruth, Angelika and Roggenbuck, Dirk and Lehmann, Werner and Schedler, Uwe and Juretzek, Thomas and Schierack, Peter}, title = {Solid-phase microbead array for multiplex O-serotyping of Escherichia coli}, series = {Microchimica Acta}, volume = {184}, journal = {Microchimica Acta}, number = {5}, issn = {0026-3672}, doi = {10.1007/s00604-017-2088-4}, pages = {1405 -- 1415}, language = {en} } @misc{BurdukiewiczSpiessBlagodatskikhetal., author = {Burdukiewicz, Michał and Spiess, Andrej-Nikolai and Blagodatskikh, Konstantin A. and Lehmann, Werner and Schierack, Peter and R{\"o}diger, Stefan}, title = {Algorithms for automated detection of hook effect-bearing amplification curves}, series = {Biomolecular Detection and Quantification}, volume = {16}, journal = {Biomolecular Detection and Quantification}, issn = {2214-7535}, doi = {10.1016/j.bdq.2018.08.001}, pages = {1 -- 4}, language = {en} } @misc{DeutschmannRoggenbuckSchierack, author = {Deutschmann, Claudia and Roggenbuck, Dirk and Schierack, Peter}, title = {The loss of tolerance to CHI3L1-A putative role in inflammatory bowel disease?}, series = {Clinical Immunology}, volume = {199}, journal = {Clinical Immunology}, issn = {1521-6616}, doi = {10/gftf2k}, pages = {12 -- 17}, language = {en} } @misc{ReddigRuebeRoedigeretal., author = {Reddig, Annika and R{\"u}be, Claudia E. and R{\"o}diger, Stefan and Schierack, Peter and Reinhold, Dirk and Roggenbuck, Dirk}, title = {DNA damage assessment and potential applications in laboratory diagnostics and precision medicine}, series = {Journal of Laboratory and Precision Medicine}, volume = {3}, journal = {Journal of Laboratory and Precision Medicine}, issn = {2519-9005}, doi = {10.21037/jlpm.2018.03.06}, pages = {15}, language = {en} } @misc{SowaReddigSchieracketal., author = {Sowa, Mandy and Reddig, Annika and Schierack, Peter and Reinhold, Dirk and Roggenbuck, Dirk}, title = {Phosphorylated histone 2AX foci determination in capillary blood mononuclear cells}, series = {Journal of Laboratory and Precision Medicine}, volume = {3}, journal = {Journal of Laboratory and Precision Medicine}, issn = {2519-9005}, doi = {10.21037/jlpm.2018.04.02}, pages = {6}, language = {en} } @misc{SchneiderWeissRuheetal., author = {Schneider, Jens and Weiss, Romano and Ruhe, Madeleine and Jung, Tobias and Roggenbuck, Dirk and Stohwasser, Ralf and Schierack, Peter and R{\"o}diger, Stefan}, title = {Open source bioimage informatic tools for the analysis of DNA damage and associated biomarkers}, series = {Journal of Laboratory and Precision Medicine}, volume = {4}, journal = {Journal of Laboratory and Precision Medicine}, issn = {2519-9005}, doi = {10.21037/jlpm.2019.04.05}, language = {en} } @misc{RuheRabeJurischkaetal., author = {Ruhe, Madeleine and Rabe, Dominik and Jurischka, Christoph and Schr{\"o}der, Julia and Schierack, Peter and Deckert, P. Markus and R{\"o}diger, Stefan}, title = {Molecular biomarkers of DNA damage in diffuse large-cell lymphoma-a review}, series = {Journal of Laboratory and Precision Medicine}, volume = {4}, journal = {Journal of Laboratory and Precision Medicine}, issn = {2519-9005}, doi = {10.21037/jlpm.2019.01.01}, pages = {20}, language = {en} } @misc{UmairMohsinAlietal., author = {Umair, Muhammad and Mohsin, Mashkoor and Ali, Qasim and Qamar, Muhammad U. and Raza, Shahbaz and Ali, Aamir and Guenther, Sebastian and Schierack, Peter}, title = {Prevalence and Genetic Relatedness of Extended Spectrum-ß-Lactamase-Producing Escherichia coli Among Humans, Cattle, and Poultry in Pakistan}, series = {Microbial drug resistance}, volume = {25}, journal = {Microbial drug resistance}, number = {9}, issn = {1931-8448}, doi = {10.1089/mdr.2018.0450}, pages = {1374 -- 1381}, language = {en} } @misc{WajidAwanSaleemietal., author = {Wajid, Muhammad and Awan, Asad Bashir and Saleemi, Muhammad Kashif and Weinreich, J{\"o}rg and Schierack, Peter and Sarwar, Yasra and Ali, Aamir}, title = {Multiple Drug Resistance and Virulence Profiling of Salmonella enterica Serovars Typhimurium and Enteritidis from Poultry Farms of Faisalabad, Pakistan}, series = {Microbial Drug Resistance}, volume = {25}, journal = {Microbial Drug Resistance}, number = {1}, issn = {1931-8448}, doi = {10.1089/mdr.2018.0121}, pages = {133 -- 142}, language = {en} } @misc{AkhtarGhauriParveenetal., author = {Akhtar, Nasrin and Ghauri, M. A. and Parveen, Sana and Farooq, M. and Ali, Aamir and Schierack, Peter}, title = {Comparative Analysis of Draft Genome Sequence of Rhodococcus sp. Eu-32 with Other Rhodococcus Species for Its Taxonomie Status and Sulfur Metabolism Potential}, series = {Current microbiology}, volume = {76}, journal = {Current microbiology}, number = {10}, issn = {1432-0991}, doi = {10.1007/s00284-019-01737-1}, pages = {1207 -- 1214}, language = {en} } @misc{OloweAdefioyeAjayeobaetal., author = {Olowe, Olugbenga Adekunle and Adefioye, Olusolabomi J. and Ajayeoba, T. A. and Schiebel, Juliane and Weinreich, J{\"o}rg and Ali, Aamir and Burdukiewicz, Michał and R{\"o}diger, Stefan and Schierack, Peter}, title = {Phylogenetic grouping and biofilm formation of Multidrug Resistant Escherichia coli Isolates from Humans, Animals and Food products in South-west Nigeria}, series = {Scientific African}, volume = {6}, journal = {Scientific African}, issn = {2468-2276}, doi = {10.1016/j.sciaf.2019.e00158}, pages = {11}, language = {en} } @misc{HerrmannRoedigerSchmidtetal., author = {Herrmann, Anna and R{\"o}diger, Stefan and Schmidt, Carsten and Schierack, Peter and Schedler, Uwe}, title = {Spatial Separation of Microbeads into Detection Levels by a Bioorthogonal Porous Hydrogel for Size-Selective Analysis und Increased Multiplexicity}, series = {Analytical chemistry}, volume = {91}, journal = {Analytical chemistry}, number = {13}, issn = {1520-6882}, doi = {10.1021/acs.analchem.9b01586.}, pages = {8484 -- 8491}, language = {en} } @misc{AwanSchiebelBoehmetal., author = {Awan, Asad Bashir and Schiebel, Juliane and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Sarwar, Yasra and Schierack, Peter and Ali, Aamir}, title = {Association of biofilm formation and cytotoxic potential with multidrug resistance in clinical isolates of Pseudomonas aeruginosa}, series = {EXCLI journal}, volume = {18}, journal = {EXCLI journal}, issn = {1611-2156}, pages = {79 -- 90}, language = {en} } @misc{DeutschmannRoggenbuckSchierack, author = {Deutschmann, Claudia and Roggenbuck, Dirk and Schierack, Peter}, title = {The loss of tolerance to CHI3L1-A putative role in inflammatory bowel disease?}, series = {Clinical Immunology}, volume = {199}, journal = {Clinical Immunology}, issn = {1521-6616}, doi = {10.1016/j.clim.2018.12.005}, pages = {12 -- 17}, language = {en} } @misc{DinterBurdukiewiczSchieracketal., author = {Dinter, Franziska and Burdukiewicz, Michał and Schierack, Peter and Lehmann, Werner and Nestler, J{\"o}rg and Dame, Gregory and R{\"o}diger, Stefan}, title = {Simultaneous detection and quantification of DNA and protein biomarkers in spectrum of cardiovascular diseases in a microfluidic microbead chip}, series = {Analytical and Bioanalytical Chemistry}, volume = {411}, journal = {Analytical and Bioanalytical Chemistry}, number = {29}, issn = {1618-2650}, doi = {10.1007/s00216-019-02199-x}, pages = {7725 -- 7735}, language = {en} } @misc{DeutschmannSowaMurugaiyanetal., author = {Deutschmann, Claudia and Sowa, Mandy and Murugaiyan, Jayaseelan and Roessler, Uwe and R{\"o}ber, Nadja and Conrad, Karsten and Laass, Martin W. and Bogdanos, Dimitrios Petrou and Sipeki, Nora and Papp, Maria and R{\"o}diger, Stefan and Roggenbuck, Dirk and Schierack, Peter}, title = {Identification of Chitinase-3-Like Protein 1 as a Novel Neutrophil Antigenic Target in Crohn's Disease}, series = {Journal of Crohn's and Colitis}, volume = {13}, journal = {Journal of Crohn's and Colitis}, number = {7}, issn = {1876-4479}, doi = {10.1093/ecco-jcc/jjz012}, pages = {894 -- 904}, abstract = {Background and Aims There is an increasing incidence of inflammatory bowel disease [IBD]. Autoimmune responses are involved in the pathophysiology of IBD, but their underlying pathways and target antigens have not yet been fully elucidated. Methods Autoantigenic targets in IBD were identified after separation of whole cell proteins isolated from neutrophils using two-dimensional electrophoresis and matrix assisted laser desorption ionization - time of flight mass spectrometry-based protein identification of the spots that displayed Western blotting signals with anti-neutrophil cytoplasmic antibody-positive sera. The prevalence of IgG, IgA and secretory IgA [sIgA] to chitinase 3-like protein 1 [CHI3L1] was analysed by enzyme-linked immunosorbent assays using recombinant CHI3L1 in 110 patients with Crohn's disease [CD], 95 with ulcerative colitis [UC], 126 with coeliac disease [CeD] and 86 healthy controls [HCs]. Results The 18-glycosylhydrolase family member CHI3L1 was identified as a neutrophil autoantigenic target. CD patients displayed significantly higher levels of IgG to CHI3L1 than patients with UC and CeD (p < 0.0001, respectively). IgA and sIgA to CHI3L1 was significantly higher in CD than in UC, CeD and HCs [p < 0.0001, respectively]. IgA and sIgA to CHI3L1 demonstrated the highest prevalence in CD [25.5\%, 28/110; and 41.8\%\%, 46/110] compared to HCs [2.3\%, 2/86; and 4.7\%\%, 4/86; p = 0.0015 and p < 0.0001] and are associated with a more complicated progression of CD. Conclusion CHI3L1 is a novel neutrophil autoantigenic target in CD. IgA and sIgA to CHI3L1 may serve as novel markers for CD and may facilitate the serological diagnosis of IBD.}, language = {en} } @misc{SowaMurugaiyanConradetal., author = {Sowa, Mandy and Murugaiyan, Jayaseelan and Conrad, Karsten and Laass, Martin W. and Bogdanos, Dimitrios Petrou and Papp, Maria and R{\"o}diger, Stefan and Roggenbuck, Dirk and Schierack, Peter}, title = {A novel neutrophil autoantigenic target in inflammatory bowel disease}, language = {en} } @misc{JurischkaDinterEfimovaetal., author = {Jurischka, Christoph and Dinter, Franziska and Efimova, Anastasia and Weiss, Romano and Schiebel, Juliane and Schulz, Christian and Fayziev, Bekzodjon and Schierack, Peter and Fischer, Thomas and R{\"o}diger, Stefan}, title = {An explorative study of polymers for 3D printing of bioanalytical test systems}, series = {Clinical Hemorheology and Microcirculation}, volume = {75}, journal = {Clinical Hemorheology and Microcirculation}, number = {1}, issn = {1875-8622}, doi = {10.3233/CH-190713}, pages = {57 -- 84}, abstract = {Background: The 3D printing is relevant as a manufacturing technology of functional models for forensic, pharmaceutical and bioanalytical applications such as drug delivery systems, sample preparation and point-of-care tests. Objective: Melting behavior and autofluorescence of materials are decisive for optimal printing and applicability of the product which are influenced by varying unknown additives. Methods: We have produced devices for bioanalytical applications from commercially available thermoplastic polymers using a melt-layer process. We characterized them by differential scanning calorimetry, fluorescence spectroscopy and functional assays (DNA capture assay, model for cell adhesion, bacterial adhesion and biofilm formation test). Results: From 14 tested colored, transparent and black materials we found only deep black acrylonitrile-butadiene-styrene (ABS) and some black polylactic acid (PLA) useable for fluorescence-based assays, with low autofluorescence only in the short-wave range of 300-400 nm. PLA was suitable for standard bioanalytical purposes due to a glass transition temperature of approximately 60°C, resistance to common laboratory chemicals and easy print processing. For temperature-critical methods, such as hybridization reactions up to 90°C, ABS was better suited. Conclusions: Autofluorescence was not a disadvantage per se but can also be used as a reference signal in assays. The rapid development of individual protocols for sample processing and analysis required the availability of a material with consistent quality over time. For fluorescence-based assays, the use of commercial standard materials did not seem to meet this requirement.}, language = {en} } @misc{LopensKrawczykPappetal., author = {Lopens, Steffi and Krawczyk, Marcin and Papp, Maria and Milkiewicz, Piotr and Schierack, Peter and Liu, Yudong and Wunsch, Ewa and Conrad, Karsten and Roggenbuck, Dirk}, title = {The search for the Holy Grail: autoantigenic targets in primary sclerosing cholangitis asscociated with diesease phenotype an neoplasia}, series = {Autoimmunity Highlights}, volume = {11}, journal = {Autoimmunity Highlights}, issn = {2038-3274}, doi = {10.1186/s13317-020-00129-x}, pages = {14}, language = {en} } @misc{SchmidtSchierackGerberetal., author = {Schmidt, Carsten and Schierack, Peter and Gerber, Ulrike and Schr{\"o}der, Christian and Choi, Youngeun and Bald, Ilko and Lehmann, Werner and R{\"o}diger, Stefan}, title = {Streptavidin Homologues for Applications on Solid Surfaces at High Temperatures}, series = {Langmuir}, volume = {36}, journal = {Langmuir}, number = {2}, issn = {1520-5827}, doi = {10.1021/acs.langmuir.9b02339}, pages = {628 -- 636}, language = {en} } @misc{VolkovSeguroLeionetal., author = {Volkov, Ilan and Seguro, Luciana and Leion, Elaine P. and Kov{\´a}cs, L{\´a}szl{\´o} and Roggenbuck, Dirk and Schierack, Peter and Gilburd, Boris and Doria, Andrea and Tektonidou, Maria G. and Agmon-Levin, Nancy}, title = {Profiles of criteria and non-criteria anti-phospholipid autoantibodies are associated with clinical phenotypes of the antiphospholipid syndrome}, series = {Autoimmunity Highlights}, volume = {11}, journal = {Autoimmunity Highlights}, issn = {2038-3274}, doi = {10.1186/s13317-020-00131-3}, pages = {1 -- 8}, language = {en} } @misc{AliKolendaKhanetal., author = {Ali, Aamir and Kolenda, Rafał and Khan, Muhammad Moman and Weinreich, J{\"o}rg and Li, Ganwu and Wieler, Lothar H. and Tedin, Karsten and Roggenbuck, Dirk and Schierack, Peter}, title = {Novel Avian Pathogenic Escherichia coli Genes Responsible for Adhesion to Chicken and Human Cell Lines}, series = {Applied and Environmental Microbiology}, volume = {86}, journal = {Applied and Environmental Microbiology}, number = {20}, issn = {1098-5336}, doi = {10.1128/AEM.01068-20}, language = {en} } @misc{SchierackHeidenKhanetal., author = {Schierack, Peter and Heiden, Stefan E. and Khan, Muhammad Moman and Nikolaus, Lena and Kolenda, Rafał and Stubbe, Michael and Lkhagvasuren, Davaa and R{\"o}diger, Stefan and Guenther, Sebastian and Schaufler, Katharina}, title = {Genomic and Phenotypic Analysis of an ESBL-Producing E. coli ST1159 Clonal Lineage From Wild Birds in Mongolia}, series = {Frontiers in Microbiology}, volume = {11}, journal = {Frontiers in Microbiology}, issn = {1664-302X}, doi = {10.3389/fmicb.2020.01699}, pages = {7}, language = {en} } @misc{ReimannZengJakopecetal., author = {Reimann, Ronny and Zeng, Bo and Jakopec, Martin and Burdukiewicz, Michał and Petrick, Ingolf and Schierack, Peter and R{\"o}diger, Stefan}, title = {Classification of dead and living microalgae Chlorella vulgaris by bioimage informatics an machine learning}, series = {Algal Research}, volume = {48}, journal = {Algal Research}, issn = {2211-9264}, doi = {10.1016/j.algal.2020.101908}, pages = {11}, language = {en} } @misc{RoggenbuckElmontReinholdetal., author = {Roggenbuck, Dirk and Elmont, Emilien and Reinhold, Dirk and Schierack, Peter and Conrad, Karsten and Boucraut, Joseph}, title = {Autoimmune Perpheral Neuropathies and Contribution of Antiganglioside/Sulphatide Autoantibody Testing}, series = {Mediterranean Jounal of Rheumatology}, volume = {31}, journal = {Mediterranean Jounal of Rheumatology}, number = {1}, issn = {2529-198X}, doi = {10.31138/mjr.31.1.10}, pages = {10 -- 18}, language = {en} } @misc{DeutschmannRoggenbuckSchieracketal., author = {Deutschmann, Claudia and Roggenbuck, Dirk and Schierack, Peter and R{\"o}diger, Stefan}, title = {Autoantibody testing by enzyme-linked immunosorbent assay-a case in which the solid phase decides on success and failure}, series = {Heliyon}, volume = {6}, journal = {Heliyon}, number = {1}, issn = {2405-8440}, doi = {10.1016/j.heliyon.2020.e03270}, pages = {6}, language = {en} } @misc{SchiebelNoackRoedigeretal., author = {Schiebel, Juliane and Noack, Jonas and R{\"o}diger, Stefan and Kammel, Anne and Menzel, Friederike and Schwibbert, Karin and Weise, Matthias and Weiss, Romano and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Elimport, Alexey and Roggenbuck, Dirk and Schierack, Peter}, title = {Analysis of three-dimensional biofilms on different material surfaces}, series = {Biomaterials Science}, volume = {8}, journal = {Biomaterials Science}, number = {12}, issn = {2047-4849}, doi = {10.1039/D0BM00455C}, pages = {3500 -- 3510}, language = {en} } @misc{LiedtkeSchroederRoggenbucketal., author = {Liedtke, Victoria and Schr{\"o}der, Christian and Roggenbuck, Dirk and Weiss, Romano and Stohwasser, Ralf and Schierack, Peter and R{\"o}diger, Stefan and Schenk, Lysann}, title = {LEDGF/p75 is required for an efficient DNA damage response}, series = {International Journal of Molecular Sciences}, volume = {22}, journal = {International Journal of Molecular Sciences}, number = {11}, doi = {10.3390/ijms22115866}, pages = {1 -- 16}, language = {en} } @misc{KolendaBurdukiewiczWimonćetal., author = {Kolenda, Rafał and Burdukiewicz, Michał and Wimonć, Marcjanna and Aleksandrowicz, Adrianna and Ali, Aamir and Szabo, Istvan and Tedin, Karsten and Bartholdson, Scott J. and Pickhard, Derek and Schierack, Peter}, title = {Identification of Natural Mutations Responsible for Altered Infection Phenotypes of Salmonella enterica Clinical Isolates by Using Cell Line Infection Screens}, series = {Applied and Environmental Microbiology}, volume = {87}, journal = {Applied and Environmental Microbiology}, number = {2}, issn = {1098-5336}, doi = {10.1128/AEM.02177-20}, language = {en} } @misc{ComabellaDeutschmannMidagliaetal., author = {Comabella, Manual and Deutschmann, Claudia and Midaglia, Luciana and Schierack, Peter and Mart{\´i}nez, J{\´u}lia and Roggenbuck, Dirk and Montalban, Xavier}, title = {Chitinase 3-like 1 is not a target antigen in patients with multiple sclerosis}, series = {Multiple Sclerosis Journal}, volume = {27}, journal = {Multiple Sclerosis Journal}, number = {9}, issn = {1477-0970}, doi = {10.1177/1352458520980141}, pages = {1455 -- 1457}, language = {en} } @misc{SchmidtBerghausBlessingetal., author = {Schmidt, Jonas and Berghaus, Sandro and Blessing, Frithjof and Wenzel, Folker and Herbeck, Holger and Blessing, Josef and Schierack, Peter and R{\"o}diger, Stefan and Roggenbuck, Dirk}, title = {Serological and viral genetic features of patients with COVID-19 in a selected German patient cohort-correlation with disease characteristics}, series = {GeroScience}, volume = {43}, journal = {GeroScience}, number = {5}, issn = {2509-2723}, doi = {10.1007/s11357-021-00443-w}, pages = {2249 -- 2264}, language = {en} } @misc{AdefioyeWeinreichRoedigeretal., author = {Adefioye, Olusolabomi J. and Weinreich, J{\"o}rg and R{\"o}diger, Stefan and Schierack, Peter and Olowe, Olugbenga Adekunle}, title = {Phylogenetic Characterization and Multilocus Sequence Typing of Extended-Spectrum Beta Lactamase-Producing Escherichia coli from Food-Producing Animals, Beef, and Humans in Southwest Nigeria}, series = {Microbial Drug Resistance}, volume = {27}, journal = {Microbial Drug Resistance}, number = {1}, issn = {1931-8448}, doi = {10.1089/mdr.2019.0397}, pages = {111 -- 120}, language = {en} } @misc{HanschmannRoedigerKrameretal., author = {Hanschmann, Henning and R{\"o}diger, Stefan and Kramer, Toni and Hanschmann, Katrin and Steidle, Michael and Fingerle, Volker and Schmidt, Carsten and Lehmann, Werner and Schierack, Peter}, title = {LoopTag FRET Probe System for Multiplex qPCR Detection of Borrelia Species}, series = {Life}, volume = {11}, journal = {Life}, number = {11}, issn = {2075-1729}, doi = {10.3390/life11111163}, language = {en} } @misc{FotangBroeringRoosetal., author = {Fotang, Chefor and Br{\"o}ring, Udo and Roos, Christian and Enoguanbhor, Evidence Chinedu and E. Abwe, Ekwoge and Dutton, Paul and Schierack, Peter and Angwafo, Tsi Evaristus and Birkhofer, Klaus}, title = {Human activity and forest fragmentation threaten populations of the Nigeria-Cameroon Chimpanzee (Pan troglodytes ellioti) in Western Cameroon}, series = {International Journal of Primatology}, volume = {42}, journal = {International Journal of Primatology}, number = {1}, issn = {1573-8604}, doi = {10.1007/s10764-020-00191-2}, pages = {105 -- 129}, abstract = {Increased human activities such as commodity-led deforestation, extension of agriculture, urbanization, and wildfires are major drivers of forest loss worldwide. In Cameroon, these activities cause a loss of suitable primate habitat and could ultimately threaten the survival of chimpanzees (Pan troglodytes). We derived independent estimates of the population size of the Endangered Nigeria-Cameroon chimpanzee (Pan troglodytes ellioti) in Kom-Wum Forest Reserve, Cameroon, and surrounding unprotected forest areas through 1) direct observations, 2) camera trapping, 3) distance sampling, 4) marked nest counts, and 5) standing crop nest counts. In addition, we georeferenced signs of chimpanzee and human activity along line transects. We used a generalized linear mixed model to predict the occurrence of chimpanzees in response to edge length (measured as the perimeter of core forest patches), core area of forest patches (measured as area of forest patches beyond an edge width of 100 m), habitat perforation (measured as the perimeter of nonforested landscape within core forest patches), patch size(measured as area of forest patches), and forest cover. Chimpanzee density estimates ranged from 0.1 (direct observation) to 0.9 (distance sampling) individuals km-2 depending on estimation method with a mean nest group size of 7 ± 5.4 (SD). The mean encounter rate for signs of chimpanzee activity was significantly higher in mature forests (2.3 signs km-1) than in secondary forests (0.3 signs km-1) and above 1000 m elevation (4.0 signs km-1) than below 1000 m (1.0 signs km-1). The mean encounter rate for signs of human activity was significantly higher in secondary (8.0 signs km-1) than in mature forests (0.9 signs km-1). Secondary forests, habitat perforation, and edge length had a significant negative effect on the occurrence of chimpanzee signs. Overall, human activity and forest degradation affected the number of observed chimpanzee signs negatively. Regular antipoaching patrols and reforestation programs in degraded areas could potentially reduce threats to populations of endangered species and may increase suitable habitat area.}, language = {en} } @misc{SchmidtBorcherdingThieleetal., author = {Schmidt, Carsten and Borcherding, Heike and Thiele, Thomas and Schedler, Uwe and Werner, Franziska and R{\"o}diger, Stefan and Roggenbuck, Dirk and Schierack, Peter}, title = {Fluorescence-encoded poly (methyl metharcylate) nanoparticles for a lateral flow assay detecting IgM autoantibodies in rheumatoid arthritis}, series = {Analytical biochemistry}, volume = {Vol. 633}, journal = {Analytical biochemistry}, issn = {1096-0309}, doi = {10.1016/journal.ppat.1010118}, language = {en} } @misc{EmmeneggerKumarEmmeneggeretal., author = {Emmenegger, Marc and Kumar, Sreedhar Saseendran and Emmenegger, Vishalini and Malinauskas, Tomas and B{\"u}ttner, Thomas and Rose, Laura and Schierack, Peter and Sprinzl, Martin F. and Sommer, Clemens J. and Lackner, Karl J. and Aguzzi, Adriano and Roggenbuck, Dirk and Frauenknecht, Katrin B. M.}, title = {Anti-prothrombin autoantibodies enriched after infection with SARS-CoV-2 and influenced by strength of antibody response against SARS-CoV-2 proteins}, series = {PLoS pathogens}, volume = {17}, journal = {PLoS pathogens}, number = {12}, issn = {1553-7374}, doi = {10.1371/journal.ppat.1010118}, language = {en} } @misc{AwanYanSarwaretal., author = {Awan, Asad Bashir and Yan, Aixin and Sarwar, Yasra and Schierack, Peter and Ali, Aamir}, title = {Detection of synergistic antimicrobial resistance mechanisms in clinical isolates of Pseudomonas aeruginosa from post-operative wound infections}, series = {Applied Microbiology and Biotechnology}, volume = {105}, journal = {Applied Microbiology and Biotechnology}, number = {2}, issn = {1432-0614}, doi = {10.1007/s00253-021-11680-6}, pages = {9321 -- 9332}, language = {en} } @misc{RoggenbuckZarskeSchieracketal., author = {Roggenbuck, Johannes J. and Zarske, Grit and Schierack, Peter and Wunderlich, Gerd and Conrad, Karsten and Kotzerke, Joerg and Roggenbuck, Dirk and Z{\"o}phel, Klaus}, title = {Third generation radioimmunoassay (RIA) for TSH receptor autoantibodies (TRAb) - one step less, similar results?}, series = {Nuklearmedizin}, volume = {60}, journal = {Nuklearmedizin}, number = {1}, issn = {0029-5566}, doi = {10.1055/a-1277-5972}, pages = {38 -- 46}, language = {en} } @misc{SchmidtBerghausBlessingetal., author = {Schmidt, Jonas and Berghaus, Sandro and Blessing, Frithjof and Wenzel, Folker and Herbeck, Holger and Blessing, Josef and Schierack, Peter and R{\"o}diger, Stefan and Roggenbuck, Dirk}, title = {A semi-automated, isolation-free, high-throughput SARS-CoV-2 reverse transcriptase (RT) loop-mediated isothermal amplification (LAMP) test}, series = {Scientific reports}, volume = {11}, journal = {Scientific reports}, number = {1}, issn = {2045-2322}, doi = {10.1038/s41598-021-00827-0}, language = {en} } @misc{BartlitzKolendaChilimoniuketal., author = {Bartlitz, Christin and Kolenda, Rafał and Chilimoniuk, Jarosław and Grzymajlo, Krzysztof and R{\"o}diger, Stefan and Bauerfeind, Rolf and Ali, Aamir and Tchesnokovag, Veronika and Roggenbuck, Dirk and Schierack, Peter}, title = {Adhesion of Enteropathogenic, Enterotoxigenic, and Commensal Escherichia coli to the Major Zymogen Granule Membrane Glyoprotein 2}, series = {Applied abd Environmental Microbiology}, volume = {88}, journal = {Applied abd Environmental Microbiology}, number = {5}, issn = {1098-5536}, doi = {10.1128/aem.02279-21}, language = {en} } @misc{SchmidtKammelTanneretal., author = {Schmidt, Carsten and Kammel, Anne and Tanner, Julian A. and Kinghorn, Andrew B. and Khan, Muhammad Moman and Lehmann, Werner and Menger, Marcus and Schedler, Uwe and Schierack, Peter and R{\"o}diger, Stefan}, title = {A Multiparametic Fluorescence Assay for Screening Aptamer-Protein Interactions Based on Microbeads}, series = {Scientific Reports}, volume = {12}, journal = {Scientific Reports}, issn = {2045-2322}, doi = {10.1038/s41598-022-06817-0}, pages = {10}, language = {en} } @misc{SydowEgerSchwabeetal., author = {Sydow, Katharina and Eger, Elias and Schwabe, Michael and Heiden, Stefan E. and Bohnert, J{\"u}rgen A. and Franzenburg, S{\"o}ren and Jurischka, Christoph and Schierack, Peter and Schaufler, Katharina}, title = {Geno- and Phenotypic Characteristics of a Klebsiella pneumoniae ST20 Isolate with Unusual Colony Morphology}, series = {Microorganisms}, volume = {10}, journal = {Microorganisms}, number = {10}, issn = {2076-2607}, doi = {10.3390/microorganisms10102063}, abstract = {Klebsiella pneumoniae is a common member of the intestinal flora of vertebrates. In addition to opportunistic representatives, hypervirulent (hvKp) and antibiotic-resistant K. pneumoniae (ABR-Kp) occur. While ABR-Kp isolates often cause difficult-to-treat diseases due to limited therapeutic options, hvKp is a pathotype that can infect healthy individuals often leading to recurrent infection. Here, we investigated the clinical K. pneumoniae isolate PBIO3459 obtained from a blood sample, which showed an unusual colony morphology. By combining whole-genome and RNA sequencing with multiple in vitro and in vivo virulence-associated assays, we aimed to define the respective Klebsiella subtype and explore the unusual phenotypic appearance. We demonstrate that PBIO3459 belongs to sequence type (ST)20 and carries no acquired resistance genes, consistent with phenotypic susceptibility tests. In addition, the isolate showed low-level virulence, both at genetic and phenotypic levels. We thus suggest that PBIO3459 is an opportunistic (commensal) K. pneumoniae isolate. Genomic comparison of PBIO3459 with closely related ABR-Kp ST20 isolates revealed that they differed only in resistance genes. Finally, the unusual colony morphology was mainly associated with carbohydrate and amino acid transport and metabolism. In conclusion, our study reveals the characteristics of a Klebsiella sepsis isolate and suggests that opportunistic representatives likely acquire and accumulate antibiotic resistances that subsequently enable their emergence as ABR-Kp pathogens.}, language = {en} } @misc{KhanSidorczukBeckeretal., author = {Khan, Muhammad Moman and Sidorczuk, Katarzyna and Becker, Juliane and Aleksandrowicz, Adrianna and Baraniewicz, Karolina and Ludwig, Christina and Ali, Aamir and Kingsley, Robert A. and Schierack, Peter and Kolenda, Rafał}, title = {Characterization of clumpy adhesion of Escherichia coli to human cells and associated factors influencing antibiotic sensitivity}, series = {Microbiology Spectrum}, journal = {Microbiology Spectrum}, issn = {2165-0497}, doi = {10.1128/spectrum.02606-23}, abstract = {Escherichia coli intestinal infection pathotypes are characterized by distinct adhesion patterns, including the recently described clumpy adhesion phenotype. Here, we identify and characterize the genetic factors contributing to the clumpy adhesion of E. coli strain 4972. In this strain, the transcriptome and proteome of adhered bacteria were found to be distinct from planktonic bacteria in the supernatant. A total of 622 genes in the transcriptome were differentially expressed in bacteria present in clumps relative to the planktonic bacteria. Seven genes targeted for disruption had variable distribution in different pathotypes and nonpathogenic E. coli, with the pilV and spnT genes being the least frequent or absent from most groups. Deletion (Δ) of five differentially expressed genes, flgH, ffp, pilV, spnT, and yggT, affected motility, adhesion, or antibiotic stress. ΔflgH exhibited 80\% decrease and ΔyggT depicted 184\% increase in adhesion, and upon complementation, adhesion was significantly reduced to 13\%. ΔflgH lost motility and was regenerated when complemented, whereas Δffp had significantly increased motility, and reintroduction of the same gene reduced it to the wild-type level. The clumps produced by Δffp and ΔspnT were more resistant and protected the bacteria, with ΔspnT showing the best clump formation in terms of ampicillin stress protection. ΔyggT had the lowest tolerance to gentamicin, where the antibiotic stress completely eliminated the bacteria. Overall, we were able to investigate the influence of clump formation on cell surface adhesion and antimicrobial tolerance, with the contribution of several factors crucial to clump formation on susceptibility to the selected antibiotics.}, language = {en} } @misc{AzamRoesslingGeitheetal., author = {Azam, Hafiz Muhammad Husnain and R{\"o}ßling, Rosa Ilse and Geithe, Christiane and Khan, Muhammad Moman and Dinter, Franziska and Hanack, Katja and Pr{\"u}ß, Harald and Husse, Britta and Roggenbuck, Dirk and Schierack, Peter and R{\"o}diger, Stefan}, title = {MicroRNA biomarkers as next-generation diagnostic tools for neurodegenerative diseases: a comprehensive review}, series = {Frontiers in Molecular Neuroscience}, volume = {17}, journal = {Frontiers in Molecular Neuroscience}, publisher = {Frontiers Media S.A.}, issn = {1662-5099}, doi = {10.3389/fnmol.2024.1386735}, pages = {35}, abstract = {Neurodegenerative diseases (NDs) are characterized by abnormalities within neurons of the brain or spinal cord that gradually lose function, eventually leading to cell death. Upon examination of affected tissue, pathological changes reveal a loss of synapses, misfolded proteins, and activation of immune cells—all indicative of disease progression—before severe clinical symptoms become apparent. Early detection of NDs is crucial for potentially administering targeted medications that may delay disease advancement. Given their complex pathophysiological features and diverse clinical symptoms, there is a pressing need for sensitive and effective diagnostic methods for NDs. Biomarkers such as microRNAs (miRNAs) have been identified as potential tools for detecting these diseases. We explore the pivotal role of miRNAs in the context of NDs, focusing on Alzheimer's disease, Parkinson's disease, Multiple sclerosis, Huntington's disease, and Amyotrophic Lateral Sclerosis. The review delves into the intricate relationship between aging and NDs, highlighting structural and functional alterations in the aging brain and their implications for disease development. It elucidates how miRNAs and RNA-binding proteins are implicated in the pathogenesis of NDs and underscores the importance of investigating their expression and function in aging. Significantly, miRNAs exert substantial influence on post-translational modifications (PTMs), impacting not just the nervous system but a wide array of tissues and cell types as well. Specific miRNAs have been found to target proteins involved in ubiquitination or de-ubiquitination processes, which play a significant role in regulating protein function and stability. We discuss the link between miRNA, PTM, and NDs. Additionally, the review discusses the significance of miRNAs as biomarkers for early disease detection, offering insights into diagnostic strategies.}, language = {en} } @misc{FrostWeissHerteletal., author = {Frost, Fabian and Weiss, Stefan and Hertel, Johannes and R{\"u}hlemann, Malte and Bang, Corinna and Franke, Andre and Nauck, Matthias and D{\"o}rr, Marcus and V{\"o}lzke, Henry and Roggenbuck, Dirk and Schierack, Peter and V{\"o}lker, Uwe and Homuth, Georg and Aghdassi, Ali A. and Sendler, Matthias and Lerch, Markus M. and Weiss, Frank U.}, title = {Fecal glycoprotein 2 is a marker of gut microbiota dysbiosis and systemic inflammation}, series = {Gut Pathogens}, volume = {16}, journal = {Gut Pathogens}, number = {1}, publisher = {Springer Science and Business Media LLC}, issn = {1757-4749}, doi = {10.1186/s13099-024-00657-1}, pages = {1 -- 11}, abstract = {Background autoantigenic glycoprotein 2 (GP2) is an important component of the innate immune system which originates from the exocrine pancreas as well as from the small intestines. The relationship of GP2 with the intestinal microbiome as well as the systemic implications of increased fecal GP2 levels are, however, still unclear. Therefore, fecal samples from 2,812 individuals of the Study of Health in Pomerania (SHIP) were collected to determine GP2 levels (enzyme-linked immunosorbent assay) and gut microbiota profiles (16 S rRNA gene sequencing). These data were correlated and associated with highly standardised and comprehensive phenotypic data of the study participants. Results Fecal GP2 levels were increased in individuals with higher body mass index and smokers, whereas lower levels were found in case of preserved exocrine pancreatic function, female sex or a healthier diet. Moreover, higher GP2 levels were associated with increased serum levels of high-sensitivity C-reactive protein, loss of gut microbial diversity and an increase of potentially detrimental bacteria (Streptococcus, Haemophilus, Clostridium XIVa, or Collinsella). At the same time, predicted microbial pathways for the biosynthesis of beneficial short-chain fatty acids or lactic acid were depleted in individuals with high fecal GP2. Of note, GP2 exhibited a stronger association to overall microbiome variation than calprotectin. Conclusion Fecal GP2 is a biomarker of gut microbiota dysbiosis and associated with increased systemic inflammation. The intestines may be more important as origin for GP2 than pancreatic acinar cells. Future studies need to investigate the potential clinical value in disease specific patient cohorts.}, language = {en} } @misc{SidorczukBurdukiewiczCerketal., author = {Sidorczuk, Katarzyna and Burdukiewicz, Michał and Cerk, Klara and Fritscher, Joachim and Kingsley, Robert A. and Schierack, Peter and Hildebrand, Falk and Kolenda, Rafał}, title = {AdhesiomeR: a tool for Escherichia coli adhesin classification and analysis}, series = {BMC Genomics}, volume = {25}, journal = {BMC Genomics}, number = {1}, publisher = {Springer Science and Business Media LLC}, issn = {1471-2164}, doi = {10.1186/s12864-024-10525-6}, pages = {1 -- 10}, abstract = {AbstractAdhesins are crucial factors in the virulence of bacterial pathogens such as Escherichia coli. However, to date no resources have been dedicated to the detailed analysis of E. coli adhesins. Here, we provide adhesiomeR software that enables characterization of the complete adhesin repertoire, termed the adhesiome. AdhesiomeR incorporates the most comprehensive database of E. coli adhesins and facilitates an extensive analysis of adhesiome. We demonstrate that adhesiomeR achieves 98\% accuracy when compared with experimental analyses. Based on analysis of 15,000 E. coli genomes, we define novel adhesiome profiles and clusters, providing a nomenclature for a unified comparison of E. coli adhesiomes.}, language = {en} }