@inproceedings{BergDueckershoffLehmannetal., author = {Berg, Heinz Peter and D{\"u}ckershoff, Roland and Lehmann, Mario and Prechavut, Nontavut}, title = {Micro Turbo-Fuel-Cell-Technology : Hybrid compact turbo machinery technology and thermodynamic aspects regarding design parameters of a high efficient MGT-SOFC-system}, series = {12th European Conference on Turbomachinery Fluid dynamics \& Thermodynamics}, booktitle = {12th European Conference on Turbomachinery Fluid dynamics \& Thermodynamics}, doi = {10.29008/ETC2017-266}, pages = {1 -- 12}, abstract = {The improvement of decentralised energy supply systems has received an important role in the recent years. One option is the combination of SOFC with recuperated MGT. Here, a new strategy for selecting the system parameters, such as the operating temperature of the SOFC, turbine inlet temperature and the pressure ratio of the hybrid system, is presented. By introducing the heat ratio between the heat required to increase the process gas to the SOFC operating temperature and the heat required to raise the SOCF exhaust gas to the TIT, it was found that high efficiency (approx. 68\%) is realisable in a compact hybrid system today. Analysis also shows that the HEX-technology is needed to improve the system effectiveness. Also, high recirculation rates with lower TIT can be considered as the future development direction. In addition, a sample configuration of an oil-free MGT rotor unit with high speed foil bearings is presented.}, language = {en} } @misc{FroemmelLehmannRoedigeretal., author = {Fr{\"o}mmel, Ulrike and Lehmann, Werner and R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Weinreich, J{\"o}rg and Groß, Julia and Roggenbuck, Dirk and Zinke, Olaf and Ansorge, Hermann and Vogel, Steffen and Klemm, Per and Wex, Thomas and Schr{\"o}der, Christian and Wieler, Lothar H. and Schierack, Peter}, title = {Adhesion of human and animal Escherichia coli strains in association with their virulence-associated genes and phylogenetic origins}, series = {Applied and environmental microbiology}, volume = {79}, journal = {Applied and environmental microbiology}, number = {19}, issn = {1098-5336}, doi = {10.1128/AEM.01384-13}, pages = {5814 -- 5829}, abstract = {Intestinal colonization is influenced by the ability of the bacterium to inhabit a niche, which is based on the expression of colonization factors. Escherichia coli carries a broad range of virulence-associated genes (VAGs) which contribute to intestinal (inVAGs) and extraintestinal (exVAGs) infection. Moreover, initial evidence indicates that inVAGs and exVAGs support intestinal colonization. We developed new screening tools to genotypically and phenotypically characterize E. coli isolates originating in humans, domestic pigs, and 17 wild mammal and avian species. We analyzed 317 isolates for the occurrence of 44 VAGs using a novel multiplex PCR microbead assay (MPMA) and for adhesion to four epithelial cell lines using a new adhesion assay. We correlated data for the definition of new adhesion genes. inVAGs were identified only sporadically, particularly in roe deer (Capreolus capreolus) and the European hedgehog ( Erinaceus europaeus). The prevalence of exVAGs depended on isolation from a specific host. Human uropathogenic E. coli isolates carried exVAGs with the highest prevalence, followed by badger (Meles meles) and roe deer isolates. Adhesion was found to be very diverse. Adhesion was specific to cells, host, and tissue, though it was also unspecific. Occurrence of the following VAGs was associated with a higher rate of adhesion to one or more cell lines: afa-dra, daaD, tsh, vat, ibeA, fyuA, mat, sfa-foc, malX, pic, irp2, and papC. In summary, we established new screening methods which enabled us to characterize large numbers of E. coli isolates. We defined reservoirs for potential pathogenic E. coli. We also identified a very broad range of colonization strategies and defined potential new adhesion genes.}, language = {en} } @incollection{RoedigerSchierackBoehmetal., author = {R{\"o}diger, Stefan and Schierack, Peter and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Berger, Ingo and Fr{\"o}mmel, Ulrike and Schmidt, Carsten and Ruhland, Mirko and Schimke, Ingolf and Roggenbuck, Dirk and Lehmann, Werner and Schr{\"o}der, Christian}, title = {A highly versatile microscope imaging technology platform for the multiplex real-time detection of biomolecules and autoimmune antibodies}, series = {Molecular diagnostics}, booktitle = {Molecular diagnostics}, editor = {Seitz, Harald and Berger, Ingo}, publisher = {Springer}, address = {Berlin [u.a.]}, isbn = {978-3-642-37690-0}, pages = {35 -- 74}, language = {en} } @misc{RoedigerLiebschSchmidtetal., author = {R{\"o}diger, Stefan and Liebsch, Claudia and Schmidt, Carsten and Lehmann, Werner and Resch-Genger, U. and Schedler, Uwe and Schierack, Peter}, title = {Nucleic acid detection based on the use of microbeads: a review}, series = {Microchimica Acta}, volume = {181}, journal = {Microchimica Acta}, number = {11-12}, issn = {0026-3672}, doi = {10.1007/s00604-014-1243-4}, pages = {1151 -- 1168}, language = {en} } @misc{RoedigerLehmannFroemmeletal., author = {R{\"o}diger, Stefan and Lehmann, Werner and Fr{\"o}mmel, Ulrike and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Burdukiewicz, Michał and Schr{\"o}der, Christian and Dangla, R{\´e}mi and Droniou, Magali and Schierack, Peter}, title = {The Modi Operandi of the VideoScan Platform for the Detection and Analysis of Nucleic Acids. Life Science Day 2013 \& 17. Leibniz Conference of Advanced Science, Freie Universit{\"a}t Berlin, 24.10.2013}, pages = {1}, language = {en} } @inproceedings{RoedigerHanschmannSteidleetal., author = {R{\"o}diger, Stefan and Hanschmann, Henning and Steidle, Michael and Schr{\"o}der, Christian and Schierack, Peter and Lehmann, Werner}, title = {Detection of Borrelia, Bordetella and HPV with the LoopTag Real-Time PCR Probe System}, series = {International journal of medical microbiology}, volume = {303}, booktitle = {International journal of medical microbiology}, number = {Supplement 1}, issn = {1618-0607}, pages = {12 -- 13}, language = {en} } @misc{LiebschRoedigerBoehmetal., author = {Liebsch, Claudia and R{\"o}diger, Stefan and B{\"o}hm, Alexander and Nitschke, J{\"o}rg and Weinreich, J{\"o}rg and Fruth, Angelika and Roggenbuck, Dirk and Lehmann, Werner and Schedler, Uwe and Juretzek, Thomas and Schierack, Peter}, title = {Solid-phase microbead array for multiplex O-serotyping of Escherichia coli}, series = {Microchimica Acta}, volume = {184}, journal = {Microchimica Acta}, number = {5}, issn = {0026-3672}, doi = {10.1007/s00604-017-2088-4}, pages = {1405 -- 1415}, language = {en} } @misc{BurdukiewiczSpiessBlagodatskikhetal., author = {Burdukiewicz, Michał and Spiess, Andrej-Nikolai and Blagodatskikh, Konstantin A. and Lehmann, Werner and Schierack, Peter and R{\"o}diger, Stefan}, title = {Algorithms for automated detection of hook effect-bearing amplification curves}, series = {Biomolecular Detection and Quantification}, volume = {16}, journal = {Biomolecular Detection and Quantification}, issn = {2214-7535}, doi = {10.1016/j.bdq.2018.08.001}, pages = {1 -- 4}, language = {en} } @misc{KruegerGengeJungKuepperetal., author = {Kr{\"u}ger-Genge, Anne and Jung, Friedrich and K{\"u}pper, Jan-Heiner and Lehmann, Christian and Franke, Ralf-Peter}, title = {Actin type and distribution in erythrocytes}, series = {Journal of Cellular Biotechnology}, volume = {3}, journal = {Journal of Cellular Biotechnology}, number = {2}, issn = {2352-3697}, doi = {10.3233/JCB-179014}, pages = {81 -- 83}, abstract = {Erythrocytes transport oxygen from the lungs to the tissues. The excess surface area together with the elasticity of the erythrocyte cell membrane provides the flexibility needed to pass through the microvasculature where the oxygen exchange occurs. Although the architecture of the red cell and its membrane-associated cytoskeletal network is known in general, the factors that control the characteristic shape change during echinocyte formation are poorly understood. In this short report we show that in echinocytes a completely reorganized membrane cytoskeleton with a box-like structure of actin filaments prevailed indicating the importance of the actin cytoskeleton during echinocyte formation.}, language = {en} } @misc{DinterBurdukiewiczSchieracketal., author = {Dinter, Franziska and Burdukiewicz, Michał and Schierack, Peter and Lehmann, Werner and Nestler, J{\"o}rg and Dame, Gregory and R{\"o}diger, Stefan}, title = {Simultaneous detection and quantification of DNA and protein biomarkers in spectrum of cardiovascular diseases in a microfluidic microbead chip}, series = {Analytical and Bioanalytical Chemistry}, volume = {411}, journal = {Analytical and Bioanalytical Chemistry}, number = {29}, issn = {1618-2650}, doi = {10.1007/s00216-019-02199-x}, pages = {7725 -- 7735}, language = {en} }