@misc{WrobelDeiningerHegemannetal., author = {Wrobel, Nadia and Deininger, Werner and Hegemann, Peter and Mirsky, Vladimir M.}, title = {Covalent immobilization of oligonucleotides on electrodes}, series = {Colloids and Surfaces B: Biointerfaces}, volume = {32}, journal = {Colloids and Surfaces B: Biointerfaces}, number = {2}, issn = {0927-7765}, doi = {10.1016/S0927-7765(03)00155-3}, pages = {157 -- 162}, abstract = {32P-labeled DNA oligomers were immobilized onto self-assembled monolayers formed by carboxy-modified alkylthiols on gold electrodes. The optimal conditions were evaluated by comparing of surface density of oligonucleotides measured for different immobilization conditions before and after desorption of physically adsorbed nucleotides. The results demonstrate that the physical adsorption depends strongly on immobilization conditions being typically 10 times higher than the chemical immobilization. Under optimal immobilization conditions, the surface density of oligonucleotides was more than 20\% of the value calculated for a densely packed monolayer.}, language = {en} } @misc{MirskyWrobelRiepletal., author = {Mirsky, Vladimir M. and Wrobel, Nadia and Riepl, Michael and Hirsch, Thomas and Wolfbeis, Otto S.}, title = {Intelligente Nanostrukturen f{\"u}r Biosensoren und Sensor-Arrays}, series = {BioTec: Zeitschrift f{\"u}r Biotechnologie}, volume = {10}, journal = {BioTec: Zeitschrift f{\"u}r Biotechnologie}, issn = {0937-2725}, pages = {28 -- 32}, language = {de} } @inproceedings{WrobelRieplSchinkingeretal., author = {Wrobel, Nadia and Riepl, Michael and Schinkinger, Manfred and Mirsky, Vladimir M. and Wolfbeis, Otto S.}, title = {Capacitive thin film biosensors for bacteriophages and nucleic acids}, series = {Proceedings, EMBEC '99, November 4 - 7, 1999, Vienna, Austria}, booktitle = {Proceedings, EMBEC '99, November 4 - 7, 1999, Vienna, Austria}, publisher = {IFMBE}, address = {Stevenage}, pages = {362 -- 363}, language = {en} } @misc{WrobelSchinkingerMirsky, author = {Wrobel, Nadia and Schinkinger, Manfred and Mirsky, Vladimir M.}, title = {A novel ultraviolet assay for testing side reactions of carbodiimides}, series = {Analytical Biochemistry}, volume = {305}, journal = {Analytical Biochemistry}, number = {2}, issn = {0003-2697}, doi = {10.1006/abio.2002.5646}, pages = {135 -- 138}, abstract = {Carbodiimides possess considerable absorbance in the ultraviolet region; the extinction coefficient of 1-ethyl-(3-dimethylaminopropyl)carbodiimide (EDC) in water is ϵ214 = 6.3 · 103 L mol-1 cm-1. It provides a very simple method for testing possible side reactions of carbodiimides. This technique was used to study effects of pH, different buffers, and other components typically present in biological samples on EDC stability. It was shown that the hydrolysis rate in pure water increased from 1.5 · 10-5 to 5 · 10-4 s-1 by pH decreasing from 7 to 4. A strong increase of the rate of EDC loss was observed in the presence of different components. This effect can be described by the following row of hydrolysis rates: citrate ⪢ acetate ≈ phosphate ⪢ SDS. The results can be used to optimize carbodiimide-mediated reactions of peptide bond formation in organic chemistry or peptide synthesis or during immobilization or cross-linking of biological molecules.}, language = {en} }