@misc{LutterLiedtkeEckeetal., author = {Lutter, Anne-Helen and Liedtke, Victoria and Ecke, Annemarie and Scholka, Jenny and Muschter, Antje and Hentschel, Erik and Becker, Roland and Anderer, Ursula}, title = {Direct co-culture of human chondrogenic microtissues with osteoblast-like Saos-2 cells positively influence the differentiation potential of cartilage cells in 3D culture}, doi = {10.1007\%2Fs10354-018-0664-9.pdf}, pages = {S. 51}, language = {en} } @misc{LutterMuschterAnderer, author = {Lutter, Anne-Helen and Muschter, Antje and Anderer, Ursula}, title = {Diagnostic Test System to detect resorption activity of osteoclasts in vitro just using small blood samples}, series = {Osteologie}, volume = {28}, journal = {Osteologie}, number = {4}, publisher = {Georg Thieme Verlag KG}, address = {Stuttgart ; New York}, issn = {2567-5818}, doi = {10.1055/s-0039-1680038}, pages = {72}, language = {en} } @inproceedings{LehmannScholkaWittenburgetal., author = {Lehmann, Mario and Scholka, Jenny and Wittenburg, Gretel and Petto, Carola and Geppert, Philipp and Muschter, Antje and Anderer, Ursula}, title = {An easily accessible adult stem cell pool may be suitable for tissue engineering-based regeneration of articular cartilage defects}, series = {eCM Conferences Open Access online periodical}, booktitle = {eCM Conferences Open Access online periodical}, isbn = {2522-235X}, pages = {P190}, language = {en} } @misc{LutterLiedtkeScholkaetal., author = {Lutter, Anne-Helen and Liedtke, Victoria and Scholka, Jenny and Muschter, Antje and Becker, Roland and Anderer, Ursula}, title = {Co-culture of human chondrogenic microtissues with osteoblast-like Saos-2 cells or HFF-1 fibroblasts influence the differentiation potential of spheroids}, series = {Journal of Cellular Biotechnology}, volume = {4}, journal = {Journal of Cellular Biotechnology}, number = {1/2}, issn = {2352-3697}, doi = {10.3233/JCB-189005}, pages = {31 -- 42}, abstract = {BACKGROUND: Tissue engineering has become a major field of research in biotechnology and biomedicine. As a consequence, cell-based therapeutic approaches are entering the hospitals, especially for skeletal regeneration. Traumatic injuries of cartilage are treated with autologous cell suspensions or in vitro generated cartilage tissues, but there is actually no therapy available for degenerative cartilage defects. However, Osteoarthritis (OA) is a major public health problem in the world affecting 240 million people globally. OBJECTIVE: To develop suitable in vitro tissues, the properties of chondrogenic spheroids should be optimized via coculture with cells naturally occurring as joint neighbours. METHODS: Human chondrocytes were isolated from condyles and propagated in monolayer culture. Scaffold-free spheroids were generated and co-cultured with joint-specific partner cells (osteoblast-like osteosarcoma cells, fibroblasts). Morphology and differentiation was analyzed using histochemistry (Alcian blue, Safranin O) and immunohistochemistry for cartilage markers (collagen type II, Sox9, proteoglycan), proliferation-associated protein (Ki67) and markers of connective tissue (collagen type I and actin). RESULTS: The provision of a more natural microenvironment in vitro via co-culture of chondrocyte-based aggregates with osteoblast-like Saos-2 cells enhanced the differentiation potential of chondrogenic spheroids towards hyaline cartilage. CONCLUSIONS: The study showed the positive influence of Saos-2 cells on the differentiation potential of human chondrocytes in co-culture.}, language = {en} }