@misc{MohsinRazaSchaufleretal., author = {Mohsin, Mashkoor and Raza, Shahbaz and Schaufler, Katharina and Roschanski, Nicole and Sarwar, Fatima and Semmler, Torsten and Schierack, Peter and Guenther, Sebastian}, title = {High Prevalence of CTX-M-15-Type ESBL-Producing E. coli from Migratory Avian Species in Pakistan}, series = {Frontiers in microbiology}, volume = {8}, journal = {Frontiers in microbiology}, number = {2476}, issn = {1664-302X}, doi = {10.3389/fmicb.2017.02476}, pages = {9}, language = {en} } @misc{MohsinGuentherSchieracketal., author = {Mohsin, Mashkoor and Guenther, Sebastian and Schierack, Peter and Tedin, Karsten and Wieler, Lothar H.}, title = {Probiotic Escherichia coli Nissle 1917 reduces growth, Shiga toxin expression, release and thus cytotoxicity of enterohemorrhagic Escherichia coli}, series = {International Journal of Medical Microbiology}, volume = {305}, journal = {International Journal of Medical Microbiology}, number = {1}, issn = {1618-0607}, doi = {10.1016/j.ijmm.2014.10.003}, pages = {20 -- 26}, language = {en} } @misc{MohsinRazaRoschanskietal., author = {Mohsin, Mashkoor and Raza, Shahbaz and Roschanski, Nicole and Guenther, Sebastian and Ali, Aamir and Schierack, Peter}, title = {Description of the First Escherichia coli Clinical Isolate Harboring the Colistin Resistance Gene mcr-1 from the Indian Subcontinent}, series = {Antimicrobial Agents and Chemotherapy}, volume = {61}, journal = {Antimicrobial Agents and Chemotherapy}, number = {1}, issn = {1098-6596}, doi = {10.1128/AAC.01945-16}, pages = {2}, language = {en} } @misc{UmairMohsinAlietal., author = {Umair, Muhammad and Mohsin, Mashkoor and Ali, Qasim and Qamar, Muhammad U. and Raza, Shahbaz and Ali, Aamir and Guenther, Sebastian and Schierack, Peter}, title = {Prevalence and Genetic Relatedness of Extended Spectrum-ß-Lactamase-Producing Escherichia coli Among Humans, Cattle, and Poultry in Pakistan}, series = {Microbial drug resistance}, volume = {25}, journal = {Microbial drug resistance}, number = {9}, issn = {1931-8448}, doi = {10.1089/mdr.2018.0450}, pages = {1374 -- 1381}, language = {en} } @misc{KhanMushtaqAbbasetal., author = {Khan, Muhammad Moman and Mushtaq, Muhammad Ahmed and Abbas, Nayyar and Fatima, Fariha and Gibbon, Marjorie J. and Schierack, Peter and Mohsin, Mashkoor}, title = {Occurrence, antimicrobial resistance and genomic features of Klebsiella pneumoniae from broiler chicken in Faisalabad, Pakistan}, series = {Frontiers in Veterinary Science}, volume = {11}, journal = {Frontiers in Veterinary Science}, publisher = {Frontiers Media S.A.}, issn = {2297-1769}, doi = {10.3389/fvets.2024.1433124}, pages = {7}, abstract = {IntroductionThe dissemination of antimicrobial resistance (AMR) in critical priority pathogens is a significant threat. Non-clinical reservoirs of AMR, such as agriculture and food production facilities, may contribute to the transmission of clinically relevant pathogens such as multidrug-resistant (MDR) Klebsiella pneumoniae. There is currently very limited knowledge regarding the population structure and genomic diversity of K. pneumoniae in poultry production in Pakistan.MethodsWe explored healthy broilers in a commercial farm from Faisalabad, Pakistan, and identified six K. pneumoniae strains from 100 broiler birds. We characterized the strains, determining clonality, virulence and antimicrobial resistance genes using next generation sequencing.ResultsThe evaluation of antimicrobial susceptibility revealed that all the strains were MDR. Genomic analysis showed that 3/6 strains belonged to ST152, harbouring acquired resistance aminoglycosides [aadA2, aph(4′)-Ia], β-lactams (blaSHV-187, blaLAP2), fosfomycin (fosA6), tetracycline (tetA), trimethoprim (dfrA12), quinolone (qnrS1), sulphonamides (sul2) and phenicol (floR). All the strains harboured the efflux pump genes oqxA, oqxB, emrR, kpnG, kpnH, kpnF, baeR, mtdB and mtdC. All six strains encoded identical virulence profiles possessing six genes, i.e., ureA, iutA, entB, allS, fimH and mrkD. Phylogenomic analysis of the dominant sequence type (ST152) present in our dataset with publicly available genomes showed that the isolates clustered to strains mainly from human sources and could pose a potential threat to food safety and public health.DiscussionThe combination of these findings with antimicrobial use data would allow a better understanding of the selective pressures that may be driving the spread of AMR. This is the first report of MDR K. pneumoniae isolated from broiler hens in Pakistan, and the finding suggests that routine surveillance of WHO critical priority pathogens in such settings would be beneficial to the development of effective control strategies to reduce AMR.}, language = {en} }