@misc{RehacekNovotnyTvarozeketal., author = {Rehacek, Vlastimil and Novotny, Ivan and Tvarozek, Vladimir and Riepl, Michael and Hirsch, T. and Mass, Markus and Schweiss, R. and Mirsky, Vladimir M. and Wolfbeis, Otto S.}, title = {Capacitive detection in thin film chemical sensors and biosensors}, series = {Advanced Semiconductor Devices and Microsystems}, journal = {Advanced Semiconductor Devices and Microsystems}, editor = {Breza, Jura and Donoval, Daniel}, publisher = {IEEE}, address = {Piscataway, NJ}, isbn = {0-7803-4909-1}, pages = {255 -- 258}, abstract = {Thin film electrode chips on silicon substrate have been realized and utilized in development of different chemo- and biosensors. These affinity sensors are based on capacitive transducing and their general structure is thin film Au/alkanethiol/receptor. Several applications of thin film gold electrodes in capacitive sensors are presented.}, language = {en} } @misc{MirskyRieplKrauseetal., author = {Mirsky, Vladimir M. and Riepl, Michael and Krause, Christian and Novotny, Ivan and Splonskowski, Markus and Rehacek, Vlastimil and Tvarozek, Vladimir and Hummel, Helmut and Wolfbeis, Otto S.}, title = {Thin film electrodes for capacitive chemo- and biosensors: an optimization of the electrodes geometry}, series = {Materials Science Forum}, volume = {287-288}, journal = {Materials Science Forum}, issn = {1662-9752}, doi = {10.4028/www.scientific.net/MSF.287-288.423}, pages = {423 -- 426}, language = {en} } @misc{MirskyVasjariNovotnyetal., author = {Mirsky, Vladimir M. and Vasjari, Majlinda and Novotny, Ivan and Rehacek, Vlastimil and Tvarozek, Vladimir and Wolfbeis, Otto S.}, title = {Self-assembled monolayers as selective filters for chemical sensors}, series = {Nanotechnology}, volume = {13}, journal = {Nanotechnology}, number = {2}, issn = {0957-4484}, pages = {175 -- 178}, abstract = {The lateral electrical conductivity of gold layers with thickness in the nanometre range was found to decrease due to adsorption of mercury, water, iodine and sulphuric compounds onto the gold surface. A self-assembled monolayer of hexadecanethiol, deposited on the gold surface, blocked the effects of water and volatile sulphuric compounds on the lateral conductivity of the gold layers, but does not block the effects of iodine and mercury. The results indicate a selective permeability of hexadecanethiol monolayers for mercury vapour, thus providing a method for development of ultrathin filters for chemical sensors.}, language = {en} } @misc{ChernyStozhkovaMirskyetal., author = {Cherny, Vladimir V. and Stozhkova, I. N. and Mirsky, Vladimir M. and Yastrebova, T. N. and Sokolov, Valeri S.}, title = {Modification of lipid bilayer properties due to sodium hypochlorite. II. Effect on model lipid systems}, series = {Biological membranes}, volume = {9}, journal = {Biological membranes}, number = {1}, issn = {0233-4755}, pages = {66 -- 73}, language = {en} } @misc{MirskyChernySokolovetal., author = {Mirsky, Vladimir M. and Cherny, Vladimir V. and Sokolov, Valeri S. and Markin, Vladislav S.}, title = {Electrostatic assay of phospholipase A activity: an application of the second harmonic method of monitoring membrane boundary potentials}, series = {Journal of Biochemical and Biophysical Methods}, volume = {21}, journal = {Journal of Biochemical and Biophysical Methods}, number = {4}, issn = {0165-022X}, pages = {277 -- 284}, abstract = {To evaluate phospholipase A activity a new assay is suggested. This assay is based on the recording of boundary potential changes of the planar bilayer lipid membrane during enzymatic hydrolysis of lipids. To register these changes, a second harmonic method is used. Sensitivity of the assay is about 0.0002 units/ml regardless of the impurities that may be present in the samples. One analysis takes about 5 min.}, language = {en} } @misc{ChernyMirskySokolovetal., author = {Cherny, Vladimir V. and Mirsky, Vladimir M. and Sokolov, Valeri S. and Markin, Vladislav S.}, title = {Determination of the phospholipase A activity}, series = {Biochemistry}, volume = {55}, journal = {Biochemistry}, number = {3}, pages = {445 -- 450}, language = {en} } @misc{MirskyChernySokolovetal., author = {Mirsky, Vladimir M. and Cherny, Vladimir V. and Sokolov, Valeri S. and Markin, Vladislav S.}, title = {Electrode for determination of the phospholipase activity based on the lipid membrane}, series = {Soviet electrochemistry}, volume = {25}, journal = {Soviet electrochemistry}, pages = {1336 -- 1340}, language = {en} } @misc{ChernyMirskySokolovetal., author = {Cherny, Vladimir V. and Mirsky, Vladimir M. and Sokolov, Valeri S. and Markin, Vladislav S.}, title = {BLM as an enzyme-sensitive electrode: determination of the phospholipase activity}, series = {Bioelectrochemistry and Bioenergetics}, volume = {21}, journal = {Bioelectrochemistry and Bioenergetics}, number = {3}, issn = {0302-4598}, pages = {373 -- 378}, language = {en} } @misc{ShishkanovaHavlikKraletal., author = {Shishkanova, Tatiana V. and Havlik, Martin and Kr{\´a}l, Vladim{\´i}r and Kopeck{\´y}, Dušan and Matějka, Pavel and Dendisov{\´a}, Marcela and Mirsky, Vladimir M.}, title = {Amino-substituted Tr{\"o}ger's base: electrochemical polymerization and characterization of the polymer film}, series = {Electrochimica Acta}, volume = {224}, journal = {Electrochimica Acta}, issn = {0013-4686}, doi = {10.1016/j.electacta.2016.12.061}, pages = {439 -- 445}, abstract = {The amino-substituted coumarin derivative of Tr{\"o}ger's base (CTB) was electrochemically polymerized on the surface of gold electrodes. The obtained polymer films were characterized by SEM, Raman spectroscopy, cyclic voltammetry and in-situ conductometry. Spectroscopic analysis indicates that the polymerization of CTB takes place via oxidation of the aniline fragment of CTB molecule, similar to the polymerization of aniline. The similarity of the polymer backbone to that of the polyaniline is also confirmed by the influence of potential on the film's conductivity, however the conductivity of the new material is in principle lower than that of polyaniline. SEM shows that the polymer forms a thin layer with a thickness of ~ 100 nm. Possible applications of the new material as an artificial receptor for aromatic analytes containing carboxy-group are proposed.}, language = {en} } @misc{VasjariShirshovSamoylovetal., author = {Vasjari, Majlinda and Shirshov, Yuri M. and Samoylov, Anton V. and Mirsky, Vladimir M.}, title = {SPR investigation of mercury reduction and oxidation on thin gold electrodes}, series = {Journal of Electroanalytical Chemistry}, volume = {605}, journal = {Journal of Electroanalytical Chemistry}, number = {1}, issn = {1572-6657}, doi = {10.1016/j.jelechem.2007.03.019}, pages = {73 -- 76}, abstract = {Mercury reduction and oxidation on ultrathin gold electrodes was investigated by simultaneous voltammetry and surface plasmon resonance (SPR). The angle dependencies of SPR signal were analyzed quantitatively. The results indicate partially reversible formation of amalgam with optical properties in between of optical properties of mercury and gold. Up to 12\% content of mercury in the gold layer is suggested.}, language = {en} } @misc{SamoylovMirskyHaoetal., author = {Samoylov, Anton V. and Mirsky, Vladimir M. and Hao, Qingli and Swart, Claudia and Shirshov, Yuri M. and Wolfbeis, Otto S.}, title = {Nanometer-thick SPR sensor for gaseous HCl}, series = {Sensors and Actuators B: Chemical}, volume = {106}, journal = {Sensors and Actuators B: Chemical}, number = {1}, issn = {0925-4005}, doi = {10.1016/j.snb.2004.08.029}, pages = {369 -- 372}, abstract = {The optical properties of electrochemically polymerized N-methylaniline are changed in the presence of gaseous hydrogen chloride. This effect was used for preparation of chemical sensors with transduction based on surface plasmon resonance. The interaction of hydrogen chloride with a two nm layer of poly-(N-methylaniline) on gold leads to the shift of the surface plasmon resonance. The analysis of the resonance spectra demonstrates that the effect is caused by an increase of the imaginary component of the refractive index; a minor contribution is also provided by film condensation leading to an increase of the real component of the refractive index and a decrease in the thickness. The effect is selective and quasi-reversible. The concentration dependence of the gas effect obeys Langmuir's adsorption isotherm with a reciprocal value of the binding constant of 850 ± 160 ppm.}, language = {en} } @misc{ZynioSamoylovSurovtsevaetal., author = {Zynio, Stepan A. and Samoylov, Anton V. and Surovtseva, Elena R. and Mirsky, Vladimir M. and Shirshov, Yuri M.}, title = {Bimetallic layers increase sensitivity of affinity sensors based on surface plasmon resonance}, series = {Sensors}, volume = {2}, journal = {Sensors}, number = {2}, issn = {1424-8220}, doi = {10.3390/s20200062}, pages = {62 -- 70}, abstract = {Two metals are used in resonant layers for chemical sensors based on surface plasmon resonance (SPR) - gold and silver. Gold displays higher shift of the resonance angle to changes of ambient refraction index and is chemically stable. Silver posses narrower resonance curve thus providing a higher signal/noise ratio of SPR chemical sensors, but has a poor chemical stability. A new structure of resonant metallic film based on bimetallic silver/gold layers (gold as an outer layer) is suggested. It combines advantages of both gold and silver resonant layers. Bimetallic resonant films display so high shift of resonance angle on changes of ambient refraction index as gold films, but show narrower resonance curve, thus providing a higher signal / noise ratio. Additionally, the outer gold layer protects silver against oxidation.}, language = {en} } @misc{BajariLindstedtRiepletal., author = {Bajari, Tarek M. and Lindstedt, Ken A. and Riepl, Michael and Mirsky, Vladimir M. and Nimpf, Johannes and Wolfbeis, Otto S. and Dresel, Hans A. and Bautz, Ekkehard K. F. and Schneider, Wolfgang Johann}, title = {A minimal binding domain of the low density lipoprotein receptor gene family}, series = {Biological Chemistry}, volume = {379}, journal = {Biological Chemistry}, number = {8-9}, issn = {1437-4315}, doi = {10.1515/bchm.1998.379.8-9.1053}, pages = {1053 -- 1062}, language = {en} } @misc{ScherbahnNizamovMirsky, author = {Scherbahn, Vitali and Nizamov, Shavkat and Mirsky, Vladimir M.}, title = {Plasmonic detection and visualization of directed adsorption of charged single nanoparticles to patterned surfaces}, series = {Microchimica Acta}, volume = {183}, journal = {Microchimica Acta}, number = {11}, issn = {1436-5073}, doi = {10.1007/s00604-016-1956-7}, pages = {2837 -- 2845}, abstract = {It has recently been shown that surface plasmon microscopy (SPM) allows single nanoparticles (NPs) on sensor surfaces to be detected and analyzed. The authors have applied this technique to study the adsorption of single metallic and plastic NPs. Binding of gold NPs (40, 60 and 100 nm in size) and of 100 nm polystyrene NPs to gold surfaces modified by differently ω-functionalized alkyl thiols was studied first. Self-assembled monolayers (SAM) with varying terminal functions including amino, carboxy, oligo(ethylene glycol), methyl, or trimethylammonium groups were deposited on gold films to form surfaces possessing different charge and hydrophobicity. The affinity of NPs to these surfaces depends strongly on the type of coating. SAMs terminated with trimethylammonium groups and carboxy group display highly different affinity and therefore were preferred when creating patterned charged surfaces. Citrate-stabilized gold NPs and sulfate-terminated polystyrene NPs were used as negatively charged NPs, while branched polyethylenimine-coated silver NPs were used as positively charged NPs. It is shown that the charged patterned areas on the gold films are capable of selectively adsorbing oppositely charged NPs that can be detected and analyzed with an ~1 ng⋅mL-1 detection limit.}, language = {en} } @misc{PaštiJanoševićLežaićĆirićMarjanovićetal., author = {Pašti, Igor A. and Janošević Ležaić, Aleksandra and Ćirić-Marjanović, Gordana and Mirsky, Vladimir M.}, title = {Resistive gas sensors based on the composites of nanostructured carbonized polyaniline and Nafion}, series = {Journal of Solid State Electrochemistry}, volume = {20}, journal = {Journal of Solid State Electrochemistry}, number = {11}, issn = {1433-0768}, doi = {10.1007/s10008-016-3344-y}, pages = {3061 -- 3069}, abstract = {Due to constant necessity to have reliable and sensitive gas sensors in many contemporary technologies, there is a permanent need for development of new sensing platforms with good sensing properties. Here, we demonstrate a novel type of resistive gas sensors based on carbonized polyaniline/Nafion composites. The sensing mechanism of such sensors is based on the sorption of gases by the composites which induce Nafion swelling and decreasing of conductivity. Chemosensitive properties can be tuned by the (i) choice of carbon materials with different conductivities, (ii) Nafion content in the composite, and (iii) thickness of the composite layer. We have shown that the sensors respond to water, acetone, ethanol, and ethanol vapors. For the last two cases, we have achieved high sensitivity, fast response, wide concentration range, and good recovery. The use of simultaneous two- and four-point techniques for these sensors provides an internal control of the sensor integrity.}, language = {en} } @misc{EfremenkoMirsky, author = {Efremenko, Yulia and Mirsky, Vladimir M.}, title = {Electrically controlled variation of receptor affinity}, series = {Analytical and Bioanalytical Chemistry}, volume = {408}, journal = {Analytical and Bioanalytical Chemistry}, number = {26}, issn = {1618-2650}, doi = {10.1007/s00216-016-9751-1}, pages = {7287}, abstract = {A concept of virtual sensor array based on electrically controlled variation of affinity properties of the receptor layer is described. It was realized on the base of integrated electrochemical chemotransistor containing polyaniline as the receptor layer. Electrical control of the redox state of polyaniline was performed in five-electrode configuration containing four electrodes for conductivity measurements and one Ag/AgCl reference electrode. All the electrodes were integrated on the same glass chip. A room-temperature ionic liquid was used for the electrical connection between the reference electrode and chemosensitive material. Conductivity measurements demonstrated effective potential-controlled electrochemical conversions of the receptor material between different redox states. Binding of trimethylamine at three different potentials, corresponding to the different states of the receptor material, was studied. Concentration dependencies and binding kinetics were analyzed. The results demonstrated that the kinetic as well as the equilibrium binding properties of the receptor layer can be controlled by electrical potential, thus providing a possibility to form a virtual sensor array using only a single sensing element.}, language = {en} } @misc{NizamovKasianMirsky, author = {Nizamov, Shavkat and Kasian, Olga and Mirsky, Vladimir M.}, title = {Individual detection and electrochemically assisted identification of adsorbed nanoparticles using surface plasmon resonance microscopy}, series = {Angewandte Chemie - International Edition}, volume = {55}, journal = {Angewandte Chemie - International Edition}, number = {25}, issn = {1521-3773}, doi = {10.1002/anie.201600853}, pages = {7247 -- 7251}, abstract = {The increasing production and application of nanoparticles necessitates a highly sensitive analytical method for the quantification and identification of these potentially hazardous materials. We describe here an application of surface plasmon microscopy for the individual detection of each adsorbed nanoparticle and for visualization of its electrochemical conversion. Whereas the adsorption rate characterizes the number concentration of nanoparticles, the potential at which the adsorbed nanoparticles disappear during an anodic potential sweep characterizes the type of material. All the adsorbed nanoparticles are subjected to the potential sweep simultaneously; nevertheless, each of the up to a million adsorbed nanoparticles is identified individually by its electrochemical dissolution potential. The technique has been tested with silver and copper nanoparticles, but can be extended to many other electrochemically active nanomaterials.}, language = {en} } @misc{InelCioateşMirskyetal., author = {Inel, Georgiana Anca and Cioate{\c{s}}, Cătălina and Mirsky, Vladimir M. and Birzan, Liviu and Ungureanu, Eleonora-Mihaela}, title = {Films obtained by electrochemistry from (6-methylazulene-1-yl)selane}, series = {UPB Scientific Bulletin, Series C: Electrical Engineering}, volume = {77}, journal = {UPB Scientific Bulletin, Series C: Electrical Engineering}, number = {2}, issn = {1454-2331}, pages = {3 -- 18}, language = {en} } @misc{JanoševićLežaićBajukBogdanovićRadoičićetal., author = {Janošević Ležaić, Aleksandra and Bajuk-Bogdanović, Danica and Radoičić, Marija and Mirsky, Vladimir M. and Ćirić-Marjanović, Gordana}, title = {Influence of synthetic conditions on the structure and electrical properties of nanofibrous polyanilines and their nanofibrous carbonized forms}, series = {Synthetic Metals}, volume = {214}, journal = {Synthetic Metals}, issn = {0379-6779}, doi = {10.1016/j.synthmet.2016.01.015}, pages = {35 -- 44}, abstract = {Nanofibrous polyanilines (PANIs) were synthesized by several oxidative polymerization pathways that have in common the presence of excess oxidant(s) (ammonium peroxydisulfate and its mixture with hydrogen peroxide), the absence of added acid, and the absence of external template (self-assembly process). Conducting forms of the synthesized PANI nanofibers (re)doped with various acids were further used as precursors for carbonization process to obtain nanofibrous carbonaceous materials (Carb-PANIs). Morphology, molecular structure, surface properties and electrical characteristics of PANI nanofibrous precursors and their carbonized counterparts were studied by scanning electron microscopy, Raman spectroscopy, by measurements of ζ-potential and determination of isoelectric points, as well as by measurements of electrical conductivity.}, language = {en} } @misc{SidorenkoNizamovHergenroederetal., author = {Sidorenko, Irina and Nizamov, Shavkat and Hergenr{\"o}der, Roland and Zybin, Alexander and Kuzmichev, Alexei and Kiwull, Bettina and Niessner, Reinhard and Mirsky, Vladimir M.}, title = {Computer assisted detection and quantification of single adsorbing nanoparticles by differential surface plasmon microscopy}, series = {Microchimica Acta}, volume = {183}, journal = {Microchimica Acta}, number = {1}, issn = {1436-5073}, doi = {10.1007/s00604-015-1599-0}, pages = {101 -- 109}, abstract = {Sensitive detection of engineered nanoparticles (NPs) in air and in liquid samples is an important task and still a major challenge in analytical chemistry. Recent work demonstrated that it can be performed using surface plasmon microscopy (SPM) where binding of single NPs to a surface leads to the formation of characteristic patterns in differential SPM images. However, these patterns have to be discriminated from a noisy background. Computer-assisted recognition of nanoparticles offers a solution but requires the development of respective tools for data analysis. Hereby a numerical method for automated detection and characterization of images of single adsorbing NPs in SPM image sequences is presented. The detection accuracy of the method was validated using computer generated images and manual counting. The method was applied for detecting and imaging of gold and silver NPs adsorbing from aqueous dispersions and for soot and NaCl NPs adsorbing from aerosols. The determined adsorption rate was in range 0.1-40 NPs per (s mm2) and linearly dependent on the concentration of nanoparticles. Depending on the type of NPs and signal to noise ratio, a probability of recognition of 90-95 \% can be achieved.}, language = {en} }