@misc{ChernySihuralidzeMirskyetal., author = {Cherny, Vladimir V. and Sihuralidze, M. G. and Mirsky, Vladimir M. and Sokolov, Valeri S.}, title = {An effect of the phospholipase A2 on the electrical potential distribution through lipid bilayer}, series = {Biological membranes}, volume = {7}, journal = {Biological membranes}, pages = {733 -- 740}, language = {en} } @misc{ChernyDonathMirskyetal., author = {Cherny, Vladimir V. and Donath, E. and Mirsky, Vladimir M. and Paulitchke, M. and Sokolov, Valeri S.}, title = {Modification of lipid bilayer properties due to sodium hypochlorite. I. Effect on red blood cell membranes}, series = {Biological membranes}, volume = {9}, journal = {Biological membranes}, number = {1}, pages = {60 -- 65}, language = {en} } @misc{MirskyMirsky, author = {Mirsky, Vladimir M. and Mirsky, Alex}, title = {Distributed reference spots in chemical sensing}, language = {en} } @misc{LyutovIvanovMirskyetal., author = {Lyutov, Vladimir V. and Ivanov, Svetlozar D. and Mirsky, Vladimir M. and Tsakova, Vessela T.}, title = {Polyaniline doped with poly(acrylamidomethylpropanesulphonic acid): electrochemical behaviour and conductive properties in neutral solutions}, series = {Chemical Papers}, volume = {67}, journal = {Chemical Papers}, number = {8}, issn = {1336-9075}, doi = {10.2478/s11696-013-0341-9}, pages = {1002 -- 1011}, abstract = {Poly(2-acrylamido-2-methyl-1-propanesulphonic acid) (PAMPSA)-doped polyaniline (PANI) layers are synthesised in the presence of sulphuric and perchloric acids. The effects of the inorganic acid as well as of the electrochemical synthetic procedure (potentiostatic and potentiodynamic deposition) and thickness of the polymer layers are studied. The focus is directed towards the pH dependence of the electrochemical redox activity and conductivity of the PAMPSA-doped PANI layers obtained under different conditions. Ascorbic acid oxidation is used as a test reaction to study the electrocatalytic behaviour of various PAMPSA-doped PANI layers in neutral solution. It is found that the type of inorganic component present in the polymerisation solution has a marked effect on the extent of doping in acidic solutions as well as on the redox electroactivity in neutral solutions. A comparison between potentiostatically and potentiodynamically synthesised layers at pH 7 shows a markedly lower conductance and lower extent of redox charge preservation in the case of potentiodynamic synthesis. The PANI electrocatalytic activity for ascorbic acid oxidation is also dependent on the polymer electrodeposition procedure, with potentiostatically synthesised layers exhibiting better electrocatalytic performance.}, language = {en} } @misc{TsakovaIvanovLangeetal., author = {Tsakova, Vessela T. and Ivanov, Svetlozar D. and Lange, Ulrich and Stoyanova, Aneliya and Lyutov, Vladimir V. and Mirsky, Vladimir M.}, title = {Electroanalytical applications of nanocomposites from conducting polymers and metallic nanoparticles prepared by layer-by-layer deposition}, series = {Pure and Applied Chemistry}, volume = {83}, journal = {Pure and Applied Chemistry}, number = {2}, issn = {1365-3075}, doi = {10.1351/PAC-CON-10-08-01}, pages = {345 -- 358}, abstract = {Layer-by-layer (LbL) deposition is a convenient technique for the formation of ultra-thin nanocomposite layers containing metallic nanoparticles (NPs) and conducting polymers (CPs). The advantages of this approach for producing composite layers suitable for electroanalytical applications are discussed. Examples of electroanalytical applications of LbL-deposited composites are presented. Composite layers consisting of polyaniline (PANI) and Pd NPs are used for hydrazine oxidation. The PANI-Au NPs system is applied for dopamine (DA) and uric acid (UA) oxidation.}, language = {en} } @misc{BroncovaAnikinLangeetal., author = {Broncov{\´a}, Gabriela and Anikin, Sergey and Lange, Ulrich and Matějka, Pavel and Krondak, Martin and Kr{\´a}l, Vladim{\´i}r and Mirsky, Vladimir M.}, title = {Electrochemical and spectroscopic properties of poly-4,4′-dialkoxy-2,2′-bipyrroles}, series = {Journal of Solid State Electrochemistry}, volume = {14}, journal = {Journal of Solid State Electrochemistry}, number = {6}, issn = {1432-8488}, doi = {10.1007/s10008-009-0911-5}, pages = {1035 -- 1044}, abstract = {The structure and the electrochemical and spectral properties of two conductive electrochemically polymerized substituted bipyrroles 4,4′-methoxy-2,2′-bipyrrole and 4,4′-buthoxy-2,2′-bipyrrole were studied and compared. The polymers were characterized by cyclic voltammetry, FT-Raman spectroscopy, scanning electron microscopy, and in situ conductivity measurements at different pH and redox state.}, language = {en} } @misc{LangeIvanovLyutovetal., author = {Lange, Ulrich and Ivanov, Svetlozar D. and Lyutov, Vladimir V. and Tsakova, Vessela T. and Mirsky, Vladimir M.}, title = {Voltammetric and conductometric behaviour of self assembled multilayer nanocomposites of polyaniline and gold nanoparticles}, series = {Journal of Solid State Electrochemistry}, volume = {14}, journal = {Journal of Solid State Electrochemistry}, number = {7}, issn = {1432-8488}, doi = {10.1007/s10008-009-0922-2}, pages = {1261 -- 1268}, abstract = {Multilayer nanocomposites from polyaniline (PANI) and gold nanoparticles (AuNPs) were formed by layer-by-layer deposition. The formation of PANI-AuNPs multilayer structures was monitored by UV-vis absorption spectroscopy and cyclic voltammetry. Each deposited bilayer of PANI-AuNPs led to a monotonous and almost linear increase in both optical absorbance and the first current peak of PANI oxidation. The prepared multilayer nanocomposites were characterized by in situ conductivity measurements at different pH and potential and by transmission electron microscopy. Finally, chemosensitive properties of the new material based on the intrinsic affinity of gold nanoparticles were studied. Changes in the film resistance on exposure to vapors of mercury and sulfur-containing compounds were observed.}, language = {en} } @misc{CiglerKralKožišeketal., author = {C{\´i}gler, Petr and Kr{\´a}l, Vladim{\´i}r and Kož{\´i}šek, Milan and Konvalinka, Jan and Mirsky, Vladimir M.}, title = {Anomalous adsorptive properties of HIV protease: Indication of two-dimensional crystallization?}, series = {Colloids and Surfaces B: Biointerfaces}, volume = {64}, journal = {Colloids and Surfaces B: Biointerfaces}, number = {1}, issn = {0927-7765}, doi = {10.1016/j.colsurfb.2008.01.011}, pages = {145 -- 149}, abstract = {Adsorption of HIV protease onto surfaces that are usually considered to be protein-resistant was studied quantitatively using surface plasmon resonance. Adsorption onto gold surfaces functionalized by OH-terminated alkyl chains was much stronger than onto oligo(ethylene glycol)-terminated surfaces. Equilibrium and kinetic adsorption constants were determined. An anomalous mutual attraction between adsorbate molecules was observed, indicating the possibility of two-dimensional crystallization of HIV protease. These results are applicable for the design of sensors/biosensors for HIV protease resistance detection and for proper manipulation of this enzyme in laboratory devices.}, language = {en} } @misc{RieplMirskyNovotnyetal., author = {Riepl, Michael and Mirsky, Vladimir M. and Novotny, Ivan and Tvarozek, Vladimir and Rehacek, Vlastimil and Wolfbeis, Otto S.}, title = {Optimization of capacitive affinity sensors: drift suppression and signal amplification}, series = {Analytica Chimica Acta}, volume = {392}, journal = {Analytica Chimica Acta}, number = {1}, issn = {0003-2670}, doi = {10.1016/S0003-2670(99)00195-6}, pages = {77 -- 84}, abstract = {The detection limit of capacitive affinity sensors based on the gold-alkanethiol system can be improved by optimization of sensor preparation and by signal amplification. The dissociation of the gold-sulfur binding is often a critical point leading to operative errors of such sensors. The stability of self-assembled monolayers prepared with different thiols on gold electrodes in aqueous and organic solvents was studied by the capacitive technique. The results show that monolayers made of 16-mercaptohexadecanoic acid are stable in aqueous solution and can be hardly extracted from a gold surface by ethanol, methanol, or dioxane, while a considerable damage of self-assembled monolayers was observed due to incubation in chloroform or dimethylformamide. In contrast, self-assembled monolayers made from short-chain disulfides or thiols (such as 3,3′-dithio-bis(propionic acid N-hydroxysuccinimide ester) or 11-mercaptoundecanoic acid) displayed a poor stability in aqueous phase. Capacitive affinity sensors based on these short-chain thiols showed a considerable drift of the signal. The use of long-chain thiols resulted in a stable signal; it was applied to compare capacitive effects due to immobilization of different biological molecules and for preparation of different biosensors. The response of capacitive biosensors can be amplified by formation of a sandwich structure. This principle was illustrated by subsequent adsorption of polyclonal anti-HSA after binding of HSA with a sensor for HSA based on monoclonal antibodies.}, language = {en} } @misc{RehacekNovotnyTvarozeketal., author = {Rehacek, Vlastimil and Novotny, Ivan and Tvarozek, Vladimir and Riepl, Michael and Hirsch, T. and Mass, Markus and Schweiss, R. and Mirsky, Vladimir M. and Wolfbeis, Otto S.}, title = {Capacitive detection in thin film chemical sensors and biosensors}, series = {Advanced Semiconductor Devices and Microsystems}, journal = {Advanced Semiconductor Devices and Microsystems}, editor = {Breza, Jura and Donoval, Daniel}, publisher = {IEEE}, address = {Piscataway, NJ}, isbn = {0-7803-4909-1}, pages = {255 -- 258}, abstract = {Thin film electrode chips on silicon substrate have been realized and utilized in development of different chemo- and biosensors. These affinity sensors are based on capacitive transducing and their general structure is thin film Au/alkanethiol/receptor. Several applications of thin film gold electrodes in capacitive sensors are presented.}, language = {en} } @misc{MirskyRieplKrauseetal., author = {Mirsky, Vladimir M. and Riepl, Michael and Krause, Christian and Novotny, Ivan and Splonskowski, Markus and Rehacek, Vlastimil and Tvarozek, Vladimir and Hummel, Helmut and Wolfbeis, Otto S.}, title = {Thin film electrodes for capacitive chemo- and biosensors: an optimization of the electrodes geometry}, series = {Materials Science Forum}, volume = {287-288}, journal = {Materials Science Forum}, issn = {1662-9752}, doi = {10.4028/www.scientific.net/MSF.287-288.423}, pages = {423 -- 426}, language = {en} } @misc{MirskyVasjariNovotnyetal., author = {Mirsky, Vladimir M. and Vasjari, Majlinda and Novotny, Ivan and Rehacek, Vlastimil and Tvarozek, Vladimir and Wolfbeis, Otto S.}, title = {Self-assembled monolayers as selective filters for chemical sensors}, series = {Nanotechnology}, volume = {13}, journal = {Nanotechnology}, number = {2}, issn = {0957-4484}, pages = {175 -- 178}, abstract = {The lateral electrical conductivity of gold layers with thickness in the nanometre range was found to decrease due to adsorption of mercury, water, iodine and sulphuric compounds onto the gold surface. A self-assembled monolayer of hexadecanethiol, deposited on the gold surface, blocked the effects of water and volatile sulphuric compounds on the lateral conductivity of the gold layers, but does not block the effects of iodine and mercury. The results indicate a selective permeability of hexadecanethiol monolayers for mercury vapour, thus providing a method for development of ultrathin filters for chemical sensors.}, language = {en} } @misc{ChernyStozhkovaMirskyetal., author = {Cherny, Vladimir V. and Stozhkova, I. N. and Mirsky, Vladimir M. and Yastrebova, T. N. and Sokolov, Valeri S.}, title = {Modification of lipid bilayer properties due to sodium hypochlorite. II. Effect on model lipid systems}, series = {Biological membranes}, volume = {9}, journal = {Biological membranes}, number = {1}, issn = {0233-4755}, pages = {66 -- 73}, language = {en} } @misc{MirskyChernySokolovetal., author = {Mirsky, Vladimir M. and Cherny, Vladimir V. and Sokolov, Valeri S. and Markin, Vladislav S.}, title = {Electrostatic assay of phospholipase A activity: an application of the second harmonic method of monitoring membrane boundary potentials}, series = {Journal of Biochemical and Biophysical Methods}, volume = {21}, journal = {Journal of Biochemical and Biophysical Methods}, number = {4}, issn = {0165-022X}, pages = {277 -- 284}, abstract = {To evaluate phospholipase A activity a new assay is suggested. This assay is based on the recording of boundary potential changes of the planar bilayer lipid membrane during enzymatic hydrolysis of lipids. To register these changes, a second harmonic method is used. Sensitivity of the assay is about 0.0002 units/ml regardless of the impurities that may be present in the samples. One analysis takes about 5 min.}, language = {en} } @misc{ChernyMirskySokolovetal., author = {Cherny, Vladimir V. and Mirsky, Vladimir M. and Sokolov, Valeri S. and Markin, Vladislav S.}, title = {Determination of the phospholipase A activity}, series = {Biochemistry}, volume = {55}, journal = {Biochemistry}, number = {3}, pages = {445 -- 450}, language = {en} } @misc{MirskyChernySokolovetal., author = {Mirsky, Vladimir M. and Cherny, Vladimir V. and Sokolov, Valeri S. and Markin, Vladislav S.}, title = {Electrode for determination of the phospholipase activity based on the lipid membrane}, series = {Soviet electrochemistry}, volume = {25}, journal = {Soviet electrochemistry}, pages = {1336 -- 1340}, language = {en} } @misc{ChernyMirskySokolovetal., author = {Cherny, Vladimir V. and Mirsky, Vladimir M. and Sokolov, Valeri S. and Markin, Vladislav S.}, title = {BLM as an enzyme-sensitive electrode: determination of the phospholipase activity}, series = {Bioelectrochemistry and Bioenergetics}, volume = {21}, journal = {Bioelectrochemistry and Bioenergetics}, number = {3}, issn = {0302-4598}, pages = {373 -- 378}, language = {en} } @misc{ShishkanovaHavlikKraletal., author = {Shishkanova, Tatiana V. and Havlik, Martin and Kr{\´a}l, Vladim{\´i}r and Kopeck{\´y}, Dušan and Matějka, Pavel and Dendisov{\´a}, Marcela and Mirsky, Vladimir M.}, title = {Amino-substituted Tr{\"o}ger's base: electrochemical polymerization and characterization of the polymer film}, series = {Electrochimica Acta}, volume = {224}, journal = {Electrochimica Acta}, issn = {0013-4686}, doi = {10.1016/j.electacta.2016.12.061}, pages = {439 -- 445}, abstract = {The amino-substituted coumarin derivative of Tr{\"o}ger's base (CTB) was electrochemically polymerized on the surface of gold electrodes. The obtained polymer films were characterized by SEM, Raman spectroscopy, cyclic voltammetry and in-situ conductometry. Spectroscopic analysis indicates that the polymerization of CTB takes place via oxidation of the aniline fragment of CTB molecule, similar to the polymerization of aniline. The similarity of the polymer backbone to that of the polyaniline is also confirmed by the influence of potential on the film's conductivity, however the conductivity of the new material is in principle lower than that of polyaniline. SEM shows that the polymer forms a thin layer with a thickness of ~ 100 nm. Possible applications of the new material as an artificial receptor for aromatic analytes containing carboxy-group are proposed.}, language = {en} } @misc{VasjariShirshovSamoylovetal., author = {Vasjari, Majlinda and Shirshov, Yuri M. and Samoylov, Anton V. and Mirsky, Vladimir M.}, title = {SPR investigation of mercury reduction and oxidation on thin gold electrodes}, series = {Journal of Electroanalytical Chemistry}, volume = {605}, journal = {Journal of Electroanalytical Chemistry}, number = {1}, issn = {1572-6657}, doi = {10.1016/j.jelechem.2007.03.019}, pages = {73 -- 76}, abstract = {Mercury reduction and oxidation on ultrathin gold electrodes was investigated by simultaneous voltammetry and surface plasmon resonance (SPR). The angle dependencies of SPR signal were analyzed quantitatively. The results indicate partially reversible formation of amalgam with optical properties in between of optical properties of mercury and gold. Up to 12\% content of mercury in the gold layer is suggested.}, language = {en} } @misc{SamoylovMirskyHaoetal., author = {Samoylov, Anton V. and Mirsky, Vladimir M. and Hao, Qingli and Swart, Claudia and Shirshov, Yuri M. and Wolfbeis, Otto S.}, title = {Nanometer-thick SPR sensor for gaseous HCl}, series = {Sensors and Actuators B: Chemical}, volume = {106}, journal = {Sensors and Actuators B: Chemical}, number = {1}, issn = {0925-4005}, doi = {10.1016/j.snb.2004.08.029}, pages = {369 -- 372}, abstract = {The optical properties of electrochemically polymerized N-methylaniline are changed in the presence of gaseous hydrogen chloride. This effect was used for preparation of chemical sensors with transduction based on surface plasmon resonance. The interaction of hydrogen chloride with a two nm layer of poly-(N-methylaniline) on gold leads to the shift of the surface plasmon resonance. The analysis of the resonance spectra demonstrates that the effect is caused by an increase of the imaginary component of the refractive index; a minor contribution is also provided by film condensation leading to an increase of the real component of the refractive index and a decrease in the thickness. The effect is selective and quasi-reversible. The concentration dependence of the gas effect obeys Langmuir's adsorption isotherm with a reciprocal value of the binding constant of 850 ± 160 ppm.}, language = {en} }