@misc{LyutovIvanovMirskyetal., author = {Lyutov, Vladimir V. and Ivanov, Svetlozar D. and Mirsky, Vladimir M. and Tsakova, Vessela T.}, title = {Polyaniline doped with poly(acrylamidomethylpropanesulphonic acid): electrochemical behaviour and conductive properties in neutral solutions}, series = {Chemical Papers}, volume = {67}, journal = {Chemical Papers}, number = {8}, issn = {1336-9075}, doi = {10.2478/s11696-013-0341-9}, pages = {1002 -- 1011}, abstract = {Poly(2-acrylamido-2-methyl-1-propanesulphonic acid) (PAMPSA)-doped polyaniline (PANI) layers are synthesised in the presence of sulphuric and perchloric acids. The effects of the inorganic acid as well as of the electrochemical synthetic procedure (potentiostatic and potentiodynamic deposition) and thickness of the polymer layers are studied. The focus is directed towards the pH dependence of the electrochemical redox activity and conductivity of the PAMPSA-doped PANI layers obtained under different conditions. Ascorbic acid oxidation is used as a test reaction to study the electrocatalytic behaviour of various PAMPSA-doped PANI layers in neutral solution. It is found that the type of inorganic component present in the polymerisation solution has a marked effect on the extent of doping in acidic solutions as well as on the redox electroactivity in neutral solutions. A comparison between potentiostatically and potentiodynamically synthesised layers at pH 7 shows a markedly lower conductance and lower extent of redox charge preservation in the case of potentiodynamic synthesis. The PANI electrocatalytic activity for ascorbic acid oxidation is also dependent on the polymer electrodeposition procedure, with potentiostatically synthesised layers exhibiting better electrocatalytic performance.}, language = {en} } @misc{TsakovaIvanovLangeetal., author = {Tsakova, Vessela T. and Ivanov, Svetlozar D. and Lange, Ulrich and Stoyanova, Aneliya and Lyutov, Vladimir V. and Mirsky, Vladimir M.}, title = {Electroanalytical applications of nanocomposites from conducting polymers and metallic nanoparticles prepared by layer-by-layer deposition}, series = {Pure and Applied Chemistry}, volume = {83}, journal = {Pure and Applied Chemistry}, number = {2}, issn = {1365-3075}, doi = {10.1351/PAC-CON-10-08-01}, pages = {345 -- 358}, abstract = {Layer-by-layer (LbL) deposition is a convenient technique for the formation of ultra-thin nanocomposite layers containing metallic nanoparticles (NPs) and conducting polymers (CPs). The advantages of this approach for producing composite layers suitable for electroanalytical applications are discussed. Examples of electroanalytical applications of LbL-deposited composites are presented. Composite layers consisting of polyaniline (PANI) and Pd NPs are used for hydrazine oxidation. The PANI-Au NPs system is applied for dopamine (DA) and uric acid (UA) oxidation.}, language = {en} } @misc{BroncovaAnikinLangeetal., author = {Broncov{\´a}, Gabriela and Anikin, Sergey and Lange, Ulrich and Matějka, Pavel and Krondak, Martin and Kr{\´a}l, Vladim{\´i}r and Mirsky, Vladimir M.}, title = {Electrochemical and spectroscopic properties of poly-4,4′-dialkoxy-2,2′-bipyrroles}, series = {Journal of Solid State Electrochemistry}, volume = {14}, journal = {Journal of Solid State Electrochemistry}, number = {6}, issn = {1432-8488}, doi = {10.1007/s10008-009-0911-5}, pages = {1035 -- 1044}, abstract = {The structure and the electrochemical and spectral properties of two conductive electrochemically polymerized substituted bipyrroles 4,4′-methoxy-2,2′-bipyrrole and 4,4′-buthoxy-2,2′-bipyrrole were studied and compared. The polymers were characterized by cyclic voltammetry, FT-Raman spectroscopy, scanning electron microscopy, and in situ conductivity measurements at different pH and redox state.}, language = {en} } @misc{LangeIvanovLyutovetal., author = {Lange, Ulrich and Ivanov, Svetlozar D. and Lyutov, Vladimir V. and Tsakova, Vessela T. and Mirsky, Vladimir M.}, title = {Voltammetric and conductometric behaviour of self assembled multilayer nanocomposites of polyaniline and gold nanoparticles}, series = {Journal of Solid State Electrochemistry}, volume = {14}, journal = {Journal of Solid State Electrochemistry}, number = {7}, issn = {1432-8488}, doi = {10.1007/s10008-009-0922-2}, pages = {1261 -- 1268}, abstract = {Multilayer nanocomposites from polyaniline (PANI) and gold nanoparticles (AuNPs) were formed by layer-by-layer deposition. The formation of PANI-AuNPs multilayer structures was monitored by UV-vis absorption spectroscopy and cyclic voltammetry. Each deposited bilayer of PANI-AuNPs led to a monotonous and almost linear increase in both optical absorbance and the first current peak of PANI oxidation. The prepared multilayer nanocomposites were characterized by in situ conductivity measurements at different pH and potential and by transmission electron microscopy. Finally, chemosensitive properties of the new material based on the intrinsic affinity of gold nanoparticles were studied. Changes in the film resistance on exposure to vapors of mercury and sulfur-containing compounds were observed.}, language = {en} } @misc{CiglerKralKožišeketal., author = {C{\´i}gler, Petr and Kr{\´a}l, Vladim{\´i}r and Kož{\´i}šek, Milan and Konvalinka, Jan and Mirsky, Vladimir M.}, title = {Anomalous adsorptive properties of HIV protease: Indication of two-dimensional crystallization?}, series = {Colloids and Surfaces B: Biointerfaces}, volume = {64}, journal = {Colloids and Surfaces B: Biointerfaces}, number = {1}, issn = {0927-7765}, doi = {10.1016/j.colsurfb.2008.01.011}, pages = {145 -- 149}, abstract = {Adsorption of HIV protease onto surfaces that are usually considered to be protein-resistant was studied quantitatively using surface plasmon resonance. Adsorption onto gold surfaces functionalized by OH-terminated alkyl chains was much stronger than onto oligo(ethylene glycol)-terminated surfaces. Equilibrium and kinetic adsorption constants were determined. An anomalous mutual attraction between adsorbate molecules was observed, indicating the possibility of two-dimensional crystallization of HIV protease. These results are applicable for the design of sensors/biosensors for HIV protease resistance detection and for proper manipulation of this enzyme in laboratory devices.}, language = {en} } @misc{RieplMirskyNovotnyetal., author = {Riepl, Michael and Mirsky, Vladimir M. and Novotny, Ivan and Tvarozek, Vladimir and Rehacek, Vlastimil and Wolfbeis, Otto S.}, title = {Optimization of capacitive affinity sensors: drift suppression and signal amplification}, series = {Analytica Chimica Acta}, volume = {392}, journal = {Analytica Chimica Acta}, number = {1}, issn = {0003-2670}, doi = {10.1016/S0003-2670(99)00195-6}, pages = {77 -- 84}, abstract = {The detection limit of capacitive affinity sensors based on the gold-alkanethiol system can be improved by optimization of sensor preparation and by signal amplification. The dissociation of the gold-sulfur binding is often a critical point leading to operative errors of such sensors. The stability of self-assembled monolayers prepared with different thiols on gold electrodes in aqueous and organic solvents was studied by the capacitive technique. The results show that monolayers made of 16-mercaptohexadecanoic acid are stable in aqueous solution and can be hardly extracted from a gold surface by ethanol, methanol, or dioxane, while a considerable damage of self-assembled monolayers was observed due to incubation in chloroform or dimethylformamide. In contrast, self-assembled monolayers made from short-chain disulfides or thiols (such as 3,3′-dithio-bis(propionic acid N-hydroxysuccinimide ester) or 11-mercaptoundecanoic acid) displayed a poor stability in aqueous phase. Capacitive affinity sensors based on these short-chain thiols showed a considerable drift of the signal. The use of long-chain thiols resulted in a stable signal; it was applied to compare capacitive effects due to immobilization of different biological molecules and for preparation of different biosensors. The response of capacitive biosensors can be amplified by formation of a sandwich structure. This principle was illustrated by subsequent adsorption of polyclonal anti-HSA after binding of HSA with a sensor for HSA based on monoclonal antibodies.}, language = {en} } @misc{RehacekNovotnyTvarozeketal., author = {Rehacek, Vlastimil and Novotny, Ivan and Tvarozek, Vladimir and Riepl, Michael and Hirsch, T. and Mass, Markus and Schweiss, R. and Mirsky, Vladimir M. and Wolfbeis, Otto S.}, title = {Capacitive detection in thin film chemical sensors and biosensors}, series = {Advanced Semiconductor Devices and Microsystems}, journal = {Advanced Semiconductor Devices and Microsystems}, editor = {Breza, Jura and Donoval, Daniel}, publisher = {IEEE}, address = {Piscataway, NJ}, isbn = {0-7803-4909-1}, pages = {255 -- 258}, abstract = {Thin film electrode chips on silicon substrate have been realized and utilized in development of different chemo- and biosensors. These affinity sensors are based on capacitive transducing and their general structure is thin film Au/alkanethiol/receptor. Several applications of thin film gold electrodes in capacitive sensors are presented.}, language = {en} } @misc{MirskyRieplKrauseetal., author = {Mirsky, Vladimir M. and Riepl, Michael and Krause, Christian and Novotny, Ivan and Splonskowski, Markus and Rehacek, Vlastimil and Tvarozek, Vladimir and Hummel, Helmut and Wolfbeis, Otto S.}, title = {Thin film electrodes for capacitive chemo- and biosensors: an optimization of the electrodes geometry}, series = {Materials Science Forum}, volume = {287-288}, journal = {Materials Science Forum}, issn = {1662-9752}, doi = {10.4028/www.scientific.net/MSF.287-288.423}, pages = {423 -- 426}, language = {en} } @misc{MirskyVasjariNovotnyetal., author = {Mirsky, Vladimir M. and Vasjari, Majlinda and Novotny, Ivan and Rehacek, Vlastimil and Tvarozek, Vladimir and Wolfbeis, Otto S.}, title = {Self-assembled monolayers as selective filters for chemical sensors}, series = {Nanotechnology}, volume = {13}, journal = {Nanotechnology}, number = {2}, issn = {0957-4484}, pages = {175 -- 178}, abstract = {The lateral electrical conductivity of gold layers with thickness in the nanometre range was found to decrease due to adsorption of mercury, water, iodine and sulphuric compounds onto the gold surface. A self-assembled monolayer of hexadecanethiol, deposited on the gold surface, blocked the effects of water and volatile sulphuric compounds on the lateral conductivity of the gold layers, but does not block the effects of iodine and mercury. The results indicate a selective permeability of hexadecanethiol monolayers for mercury vapour, thus providing a method for development of ultrathin filters for chemical sensors.}, language = {en} } @misc{ChernyStozhkovaMirskyetal., author = {Cherny, Vladimir V. and Stozhkova, I. N. and Mirsky, Vladimir M. and Yastrebova, T. N. and Sokolov, Valeri S.}, title = {Modification of lipid bilayer properties due to sodium hypochlorite. II. Effect on model lipid systems}, series = {Biological membranes}, volume = {9}, journal = {Biological membranes}, number = {1}, issn = {0233-4755}, pages = {66 -- 73}, language = {en} } @misc{MirskyChernySokolovetal., author = {Mirsky, Vladimir M. and Cherny, Vladimir V. and Sokolov, Valeri S. and Markin, Vladislav S.}, title = {Electrostatic assay of phospholipase A activity: an application of the second harmonic method of monitoring membrane boundary potentials}, series = {Journal of Biochemical and Biophysical Methods}, volume = {21}, journal = {Journal of Biochemical and Biophysical Methods}, number = {4}, issn = {0165-022X}, pages = {277 -- 284}, abstract = {To evaluate phospholipase A activity a new assay is suggested. This assay is based on the recording of boundary potential changes of the planar bilayer lipid membrane during enzymatic hydrolysis of lipids. To register these changes, a second harmonic method is used. Sensitivity of the assay is about 0.0002 units/ml regardless of the impurities that may be present in the samples. One analysis takes about 5 min.}, language = {en} } @misc{ChernyMirskySokolovetal., author = {Cherny, Vladimir V. and Mirsky, Vladimir M. and Sokolov, Valeri S. and Markin, Vladislav S.}, title = {Determination of the phospholipase A activity}, series = {Biochemistry}, volume = {55}, journal = {Biochemistry}, number = {3}, pages = {445 -- 450}, language = {en} } @misc{MirskyChernySokolovetal., author = {Mirsky, Vladimir M. and Cherny, Vladimir V. and Sokolov, Valeri S. and Markin, Vladislav S.}, title = {Electrode for determination of the phospholipase activity based on the lipid membrane}, series = {Soviet electrochemistry}, volume = {25}, journal = {Soviet electrochemistry}, pages = {1336 -- 1340}, language = {en} } @misc{ChernyMirskySokolovetal., author = {Cherny, Vladimir V. and Mirsky, Vladimir M. and Sokolov, Valeri S. and Markin, Vladislav S.}, title = {BLM as an enzyme-sensitive electrode: determination of the phospholipase activity}, series = {Bioelectrochemistry and Bioenergetics}, volume = {21}, journal = {Bioelectrochemistry and Bioenergetics}, number = {3}, issn = {0302-4598}, pages = {373 -- 378}, language = {en} } @misc{ShishkanovaHavlikKraletal., author = {Shishkanova, Tatiana V. and Havlik, Martin and Kr{\´a}l, Vladim{\´i}r and Kopeck{\´y}, Dušan and Matějka, Pavel and Dendisov{\´a}, Marcela and Mirsky, Vladimir M.}, title = {Amino-substituted Tr{\"o}ger's base: electrochemical polymerization and characterization of the polymer film}, series = {Electrochimica Acta}, volume = {224}, journal = {Electrochimica Acta}, issn = {0013-4686}, doi = {10.1016/j.electacta.2016.12.061}, pages = {439 -- 445}, abstract = {The amino-substituted coumarin derivative of Tr{\"o}ger's base (CTB) was electrochemically polymerized on the surface of gold electrodes. The obtained polymer films were characterized by SEM, Raman spectroscopy, cyclic voltammetry and in-situ conductometry. Spectroscopic analysis indicates that the polymerization of CTB takes place via oxidation of the aniline fragment of CTB molecule, similar to the polymerization of aniline. The similarity of the polymer backbone to that of the polyaniline is also confirmed by the influence of potential on the film's conductivity, however the conductivity of the new material is in principle lower than that of polyaniline. SEM shows that the polymer forms a thin layer with a thickness of ~ 100 nm. Possible applications of the new material as an artificial receptor for aromatic analytes containing carboxy-group are proposed.}, language = {en} } @misc{VasjariShirshovSamoylovetal., author = {Vasjari, Majlinda and Shirshov, Yuri M. and Samoylov, Anton V. and Mirsky, Vladimir M.}, title = {SPR investigation of mercury reduction and oxidation on thin gold electrodes}, series = {Journal of Electroanalytical Chemistry}, volume = {605}, journal = {Journal of Electroanalytical Chemistry}, number = {1}, issn = {1572-6657}, doi = {10.1016/j.jelechem.2007.03.019}, pages = {73 -- 76}, abstract = {Mercury reduction and oxidation on ultrathin gold electrodes was investigated by simultaneous voltammetry and surface plasmon resonance (SPR). The angle dependencies of SPR signal were analyzed quantitatively. The results indicate partially reversible formation of amalgam with optical properties in between of optical properties of mercury and gold. Up to 12\% content of mercury in the gold layer is suggested.}, language = {en} } @misc{SamoylovMirskyHaoetal., author = {Samoylov, Anton V. and Mirsky, Vladimir M. and Hao, Qingli and Swart, Claudia and Shirshov, Yuri M. and Wolfbeis, Otto S.}, title = {Nanometer-thick SPR sensor for gaseous HCl}, series = {Sensors and Actuators B: Chemical}, volume = {106}, journal = {Sensors and Actuators B: Chemical}, number = {1}, issn = {0925-4005}, doi = {10.1016/j.snb.2004.08.029}, pages = {369 -- 372}, abstract = {The optical properties of electrochemically polymerized N-methylaniline are changed in the presence of gaseous hydrogen chloride. This effect was used for preparation of chemical sensors with transduction based on surface plasmon resonance. The interaction of hydrogen chloride with a two nm layer of poly-(N-methylaniline) on gold leads to the shift of the surface plasmon resonance. The analysis of the resonance spectra demonstrates that the effect is caused by an increase of the imaginary component of the refractive index; a minor contribution is also provided by film condensation leading to an increase of the real component of the refractive index and a decrease in the thickness. The effect is selective and quasi-reversible. The concentration dependence of the gas effect obeys Langmuir's adsorption isotherm with a reciprocal value of the binding constant of 850 ± 160 ppm.}, language = {en} } @misc{ZynioSamoylovSurovtsevaetal., author = {Zynio, Stepan A. and Samoylov, Anton V. and Surovtseva, Elena R. and Mirsky, Vladimir M. and Shirshov, Yuri M.}, title = {Bimetallic layers increase sensitivity of affinity sensors based on surface plasmon resonance}, series = {Sensors}, volume = {2}, journal = {Sensors}, number = {2}, issn = {1424-8220}, doi = {10.3390/s20200062}, pages = {62 -- 70}, abstract = {Two metals are used in resonant layers for chemical sensors based on surface plasmon resonance (SPR) - gold and silver. Gold displays higher shift of the resonance angle to changes of ambient refraction index and is chemically stable. Silver posses narrower resonance curve thus providing a higher signal/noise ratio of SPR chemical sensors, but has a poor chemical stability. A new structure of resonant metallic film based on bimetallic silver/gold layers (gold as an outer layer) is suggested. It combines advantages of both gold and silver resonant layers. Bimetallic resonant films display so high shift of resonance angle on changes of ambient refraction index as gold films, but show narrower resonance curve, thus providing a higher signal / noise ratio. Additionally, the outer gold layer protects silver against oxidation.}, language = {en} } @misc{BajariLindstedtRiepletal., author = {Bajari, Tarek M. and Lindstedt, Ken A. and Riepl, Michael and Mirsky, Vladimir M. and Nimpf, Johannes and Wolfbeis, Otto S. and Dresel, Hans A. and Bautz, Ekkehard K. F. and Schneider, Wolfgang Johann}, title = {A minimal binding domain of the low density lipoprotein receptor gene family}, series = {Biological Chemistry}, volume = {379}, journal = {Biological Chemistry}, number = {8-9}, issn = {1437-4315}, doi = {10.1515/bchm.1998.379.8-9.1053}, pages = {1053 -- 1062}, language = {en} } @misc{ScherbahnNizamovMirsky, author = {Scherbahn, Vitali and Nizamov, Shavkat and Mirsky, Vladimir M.}, title = {Plasmonic detection and visualization of directed adsorption of charged single nanoparticles to patterned surfaces}, series = {Microchimica Acta}, volume = {183}, journal = {Microchimica Acta}, number = {11}, issn = {1436-5073}, doi = {10.1007/s00604-016-1956-7}, pages = {2837 -- 2845}, abstract = {It has recently been shown that surface plasmon microscopy (SPM) allows single nanoparticles (NPs) on sensor surfaces to be detected and analyzed. The authors have applied this technique to study the adsorption of single metallic and plastic NPs. Binding of gold NPs (40, 60 and 100 nm in size) and of 100 nm polystyrene NPs to gold surfaces modified by differently ω-functionalized alkyl thiols was studied first. Self-assembled monolayers (SAM) with varying terminal functions including amino, carboxy, oligo(ethylene glycol), methyl, or trimethylammonium groups were deposited on gold films to form surfaces possessing different charge and hydrophobicity. The affinity of NPs to these surfaces depends strongly on the type of coating. SAMs terminated with trimethylammonium groups and carboxy group display highly different affinity and therefore were preferred when creating patterned charged surfaces. Citrate-stabilized gold NPs and sulfate-terminated polystyrene NPs were used as negatively charged NPs, while branched polyethylenimine-coated silver NPs were used as positively charged NPs. It is shown that the charged patterned areas on the gold films are capable of selectively adsorbing oppositely charged NPs that can be detected and analyzed with an ~1 ng⋅mL-1 detection limit.}, language = {en} } @misc{PaštiJanoševićLežaićĆirićMarjanovićetal., author = {Pašti, Igor A. and Janošević Ležaić, Aleksandra and Ćirić-Marjanović, Gordana and Mirsky, Vladimir M.}, title = {Resistive gas sensors based on the composites of nanostructured carbonized polyaniline and Nafion}, series = {Journal of Solid State Electrochemistry}, volume = {20}, journal = {Journal of Solid State Electrochemistry}, number = {11}, issn = {1433-0768}, doi = {10.1007/s10008-016-3344-y}, pages = {3061 -- 3069}, abstract = {Due to constant necessity to have reliable and sensitive gas sensors in many contemporary technologies, there is a permanent need for development of new sensing platforms with good sensing properties. Here, we demonstrate a novel type of resistive gas sensors based on carbonized polyaniline/Nafion composites. The sensing mechanism of such sensors is based on the sorption of gases by the composites which induce Nafion swelling and decreasing of conductivity. Chemosensitive properties can be tuned by the (i) choice of carbon materials with different conductivities, (ii) Nafion content in the composite, and (iii) thickness of the composite layer. We have shown that the sensors respond to water, acetone, ethanol, and ethanol vapors. For the last two cases, we have achieved high sensitivity, fast response, wide concentration range, and good recovery. The use of simultaneous two- and four-point techniques for these sensors provides an internal control of the sensor integrity.}, language = {en} } @misc{EfremenkoMirsky, author = {Efremenko, Yulia and Mirsky, Vladimir M.}, title = {Electrically controlled variation of receptor affinity}, series = {Analytical and Bioanalytical Chemistry}, volume = {408}, journal = {Analytical and Bioanalytical Chemistry}, number = {26}, issn = {1618-2650}, doi = {10.1007/s00216-016-9751-1}, pages = {7287}, abstract = {A concept of virtual sensor array based on electrically controlled variation of affinity properties of the receptor layer is described. It was realized on the base of integrated electrochemical chemotransistor containing polyaniline as the receptor layer. Electrical control of the redox state of polyaniline was performed in five-electrode configuration containing four electrodes for conductivity measurements and one Ag/AgCl reference electrode. All the electrodes were integrated on the same glass chip. A room-temperature ionic liquid was used for the electrical connection between the reference electrode and chemosensitive material. Conductivity measurements demonstrated effective potential-controlled electrochemical conversions of the receptor material between different redox states. Binding of trimethylamine at three different potentials, corresponding to the different states of the receptor material, was studied. Concentration dependencies and binding kinetics were analyzed. The results demonstrated that the kinetic as well as the equilibrium binding properties of the receptor layer can be controlled by electrical potential, thus providing a possibility to form a virtual sensor array using only a single sensing element.}, language = {en} } @misc{NizamovKasianMirsky, author = {Nizamov, Shavkat and Kasian, Olga and Mirsky, Vladimir M.}, title = {Individual detection and electrochemically assisted identification of adsorbed nanoparticles using surface plasmon resonance microscopy}, series = {Angewandte Chemie - International Edition}, volume = {55}, journal = {Angewandte Chemie - International Edition}, number = {25}, issn = {1521-3773}, doi = {10.1002/anie.201600853}, pages = {7247 -- 7251}, abstract = {The increasing production and application of nanoparticles necessitates a highly sensitive analytical method for the quantification and identification of these potentially hazardous materials. We describe here an application of surface plasmon microscopy for the individual detection of each adsorbed nanoparticle and for visualization of its electrochemical conversion. Whereas the adsorption rate characterizes the number concentration of nanoparticles, the potential at which the adsorbed nanoparticles disappear during an anodic potential sweep characterizes the type of material. All the adsorbed nanoparticles are subjected to the potential sweep simultaneously; nevertheless, each of the up to a million adsorbed nanoparticles is identified individually by its electrochemical dissolution potential. The technique has been tested with silver and copper nanoparticles, but can be extended to many other electrochemically active nanomaterials.}, language = {en} } @misc{InelCioateşMirskyetal., author = {Inel, Georgiana Anca and Cioate{\c{s}}, Cătălina and Mirsky, Vladimir M. and Birzan, Liviu and Ungureanu, Eleonora-Mihaela}, title = {Films obtained by electrochemistry from (6-methylazulene-1-yl)selane}, series = {UPB Scientific Bulletin, Series C: Electrical Engineering}, volume = {77}, journal = {UPB Scientific Bulletin, Series C: Electrical Engineering}, number = {2}, issn = {1454-2331}, pages = {3 -- 18}, language = {en} } @misc{JanoševićLežaićBajukBogdanovićRadoičićetal., author = {Janošević Ležaić, Aleksandra and Bajuk-Bogdanović, Danica and Radoičić, Marija and Mirsky, Vladimir M. and Ćirić-Marjanović, Gordana}, title = {Influence of synthetic conditions on the structure and electrical properties of nanofibrous polyanilines and their nanofibrous carbonized forms}, series = {Synthetic Metals}, volume = {214}, journal = {Synthetic Metals}, issn = {0379-6779}, doi = {10.1016/j.synthmet.2016.01.015}, pages = {35 -- 44}, abstract = {Nanofibrous polyanilines (PANIs) were synthesized by several oxidative polymerization pathways that have in common the presence of excess oxidant(s) (ammonium peroxydisulfate and its mixture with hydrogen peroxide), the absence of added acid, and the absence of external template (self-assembly process). Conducting forms of the synthesized PANI nanofibers (re)doped with various acids were further used as precursors for carbonization process to obtain nanofibrous carbonaceous materials (Carb-PANIs). Morphology, molecular structure, surface properties and electrical characteristics of PANI nanofibrous precursors and their carbonized counterparts were studied by scanning electron microscopy, Raman spectroscopy, by measurements of ζ-potential and determination of isoelectric points, as well as by measurements of electrical conductivity.}, language = {en} } @misc{SidorenkoNizamovHergenroederetal., author = {Sidorenko, Irina and Nizamov, Shavkat and Hergenr{\"o}der, Roland and Zybin, Alexander and Kuzmichev, Alexei and Kiwull, Bettina and Niessner, Reinhard and Mirsky, Vladimir M.}, title = {Computer assisted detection and quantification of single adsorbing nanoparticles by differential surface plasmon microscopy}, series = {Microchimica Acta}, volume = {183}, journal = {Microchimica Acta}, number = {1}, issn = {1436-5073}, doi = {10.1007/s00604-015-1599-0}, pages = {101 -- 109}, abstract = {Sensitive detection of engineered nanoparticles (NPs) in air and in liquid samples is an important task and still a major challenge in analytical chemistry. Recent work demonstrated that it can be performed using surface plasmon microscopy (SPM) where binding of single NPs to a surface leads to the formation of characteristic patterns in differential SPM images. However, these patterns have to be discriminated from a noisy background. Computer-assisted recognition of nanoparticles offers a solution but requires the development of respective tools for data analysis. Hereby a numerical method for automated detection and characterization of images of single adsorbing NPs in SPM image sequences is presented. The detection accuracy of the method was validated using computer generated images and manual counting. The method was applied for detecting and imaging of gold and silver NPs adsorbing from aqueous dispersions and for soot and NaCl NPs adsorbing from aerosols. The determined adsorption rate was in range 0.1-40 NPs per (s mm2) and linearly dependent on the concentration of nanoparticles. Depending on the type of NPs and signal to noise ratio, a probability of recognition of 90-95 \% can be achieved.}, language = {en} } @misc{NizamovScherbahnMirsky, author = {Nizamov, Shavkat and Scherbahn, Vitali and Mirsky, Vladimir M.}, title = {Self-referencing SPR-sensor based on integral measurements of light intensity reflected by arbitrarily distributed sensing and referencing spots}, series = {Sensors and Actuators B: Chemical}, volume = {207}, journal = {Sensors and Actuators B: Chemical}, number = {A}, issn = {0925-4005}, doi = {10.1016/j.snb.2014.10.022}, pages = {740 -- 747}, abstract = {A new approach for self-referencing in SPR biosensors is reported. The method is technologically simple and applicable for a wide range of existing SPR instrumentation with Kretschmann configuration. It is based on the micropatterning of the sensor area with sensing and referencing areas whose shape and distribution can be chosen arbitrarily and their characteristic sizes are larger than the plasmons propagation length. Provided that roughly a half of the area is used as the sensing area while its optical thickness is different from that of the referencing area, an integral measurement of the intensity of the reflected light over such a patterned surface near the summary resonance conditions exhibits self-referencing properties. An over ten-fold suppression of the effect caused by the variation of the bulk refractive index was observed.}, language = {en} } @misc{KlemmJohnsonMirsky, author = {Klemm, Franziska and Johnson, Richard and Mirsky, Vladimir M.}, title = {Binding of protein nanoparticles to immobilized receptors}, series = {Sensors and Actuators B: Chemical}, volume = {208}, journal = {Sensors and Actuators B: Chemical}, issn = {0925-4005}, doi = {10.1016/j.snb.2014.10.135}, pages = {616 -- 621}, abstract = {The interaction of nanoparticles manufactured from human serum albumin (HSA) with different types of receptor surfaces was studied. Four different immobilization techniques were used to chemically link the receptors to the detector surfaces, namely: (i) a surface coated by monoclonal antibodies by a single step immobilization technique using carbodiimide, (ii) a surface coated by the same technique but using polyclonal antibodies, (iii) a surface coated by monoclonal antibodies immobilized through the sugar groups of antibodies and (iv) a surface coated by polyclonal antibodies immobilized by the same technique as in (iii). The results showed that there was almost no difference in the affinity of HSA in the molecular form to all of these surfaces. However, the binding behaviour of nanoparticles made from the same protein was quite different as successful nanoparticle binding was only observed when the surfaces were produced using method (ii).}, language = {en} } @misc{WolfbeisMirskyRiepl, author = {Wolfbeis, Otto S. and Mirsky, Vladimir M. and Riepl, Michael}, title = {Method for producing laterally organized structures on supporting surfaces}, language = {en} } @misc{AndoWolfbeisMirsky, author = {Ando, Masanori and Wolfbeis, Otto S. and Mirsky, Vladimir M.}, title = {Ozone detecting material and method for detecting ozone}, language = {en} } @misc{MirskyLange, author = {Mirsky, Vladimir M. and Lange, Ulrich}, title = {Multielectrode chemoresistor}, language = {en} } @misc{DrexlerShishkanovaLangeetal., author = {Drexler, Christoph and Shishkanova, Tatiana V. and Lange, Christoph and Danilov, Sergey N. and Weiss, Dieter and Ganichev, Sergey D. and Mirsky, Vladimir M.}, title = {Terahertz split-ring metamaterials as transducers for chemical sensors based on conducting polymers: a feasibility study with sensing of acidic and basic gases using polyaniline chemosensitive layer}, series = {Microchimica Acta}, volume = {181}, journal = {Microchimica Acta}, issn = {1436-5073}, doi = {10.5283/epub.31493}, pages = {1857 -- 1862}, abstract = {We report on the first application of terahertz metamaterials acting as transducers for chemical sensors based on conducting polymers. In our feasibility study aimed at sensing of gaseous hydrochloric and ammonia, a two-dimensional sensor metamaterial consisting of an array of split-ring resonators on the surface of undoped silicon wafer was prepared. The surface of the resonator was coated with a 150-μm layer of polyaniline. Binding of hydrogen chloride to polyaniline leads to distinct changes in the resonance frequency of the metamaterial. Measurements can be performed both in the reflection and transmission mode. A numerical simulation of the response revealed an increase of both the real and the imaginary components of the dielectric function of the polyaniline film. These changes are attributed to the transition from emaraldine base to emeraldine salt. The results demonstrate a new approach for formation of highly sensitive transducers for chemical sensors.}, language = {en} } @misc{BarigSchiemannMirskyetal., author = {Barig, Susann and Schiemann, Manja and Mirsky, Vladimir M. and Stahmann, Klaus-Peter}, title = {Quantitative turbidity assay for lipolytic enzymes in microtiter plates}, series = {Analytical and Bioanalytical Chemistry}, volume = {405}, journal = {Analytical and Bioanalytical Chemistry}, number = {26}, issn = {1618-2650}, doi = {10.1007/s00216-013-7283-5}, pages = {8539 -- 8547}, abstract = {A clearing assay for lipolytic enzymes has been realized in 96-well microtiter plates. A thin layer containing emulsified tributyrin as turbidity-generating substrate was placed on a thicker supporting aqueous layer. Both layers were stabilized by a gel-forming agent. Enzyme addition leads to clearing of the emulsion detected with a standard microtiter plate reader as a decrease of extinction. Dependencies of the signal kinetics on the substrate and enzyme concentrations were studied. For 0.5-1 \% tributyrin content the reaction rate is not substrate-limited. An initial slope of the signal kinetics is proportional to the lipase activity. A detailed characterization of the assay was performed. Lipolysis of tributyrin was confirmed by glycerol detection. Various gel-forming agents were compared and diffusion conditions in these gels were analyzed. Agar and agarose were found to be the most suitable gel-forming agents, which do not affect enzyme diffusion whereas polyacrylamide gels block lipase diffusion and therefore are not suitable for the assay. The optimized assay prepared from 1 \% tributyrin emulsion in 2 \% agar gel was tested with six microbial lipases and porcine pancreatic lipase. The detection limit is 20-60 ng/well which is equivalent to 30 μU/well for T. lanuginosus lipase.}, language = {en} } @misc{LangeMirsky, author = {Lange, Ulrich and Mirsky, Vladimir M.}, title = {Electroanalytical measurements without electrolytes: Conducting polymers as probes for redox titration in non-conductive organic media}, series = {Analytica Chimica Acta}, volume = {744}, journal = {Analytica Chimica Acta}, issn = {0003-2670}, doi = {10.1016/j.aca.2012.07.024}, pages = {29 -- 32}, abstract = {Electroanalytical methods have been applied only in conducting media. An application of conducting polymers allows to overcome this limitation. If such material is in electrochemical equilibrium with dissolved redox active species, its electrical conductivity depends on the redox potential of these species. Therefore, conductometric measurements with conducting polymers can provide about the same information as classical redox electrodes. The approach was applied for redox titration. Equivalent points obtained by this titration in aqueous and organic electrolytes were identical. Then the approach was applied for determination of bromine number by redox titration in non-conducting organic phase.}, language = {en} } @misc{LangeMirsky, author = {Lange, Ulrich and Mirsky, Vladimir M.}, title = {Polythiophene films on gold electrodes: A comparison of bulk and contact resistances in aqueous and organic media}, series = {Journal of Solid State Electrochemistry}, volume = {15}, journal = {Journal of Solid State Electrochemistry}, number = {11}, issn = {1433-0768}, doi = {10.1007/s10008-011-1450-4}, pages = {2377 -- 2382}, abstract = {Recently, developed technique for separated analysis of bulk and contact resistance was applied for the investigation of polythiophene films electropolymerized in boron trifluoride diethylether. Kinetics of polymer resistance and for the first time of the contact resistance during polymer oxidation and reduction were characterized. Influence of electrochemically controlled oxidation state on the polymer bulk and the polymer/metal contact resistance was measured in aqueous and organic environment. Variation of the electrical potential from -0.2 to 1.1 V vs. Ag/AgCl (sat) leads to an increase of the polymer conductivity for about three orders of magnitude and to a decrease of the contact resistance for about three orders of magnitude. The potential dependence of the two resistances was different, especially at high anodic potentials. In organic solution, the change of both resistances was more than six orders of magnitude. The results were compared with electrochemical and spectroelectrochemical data, a difference in the material behavior depending on the electrolyte solvent was observed. The influence of electrical potential on polymer resistance in aqueous solution was explained quantitatively by a three-state model with the values of oxidation potential +0.3 and +1.2 V.}, language = {en} } @misc{NizamovMirsky, author = {Nizamov, Shavkat and Mirsky, Vladimir M.}, title = {Self-referencing SPR-biosensors based on penetration difference of evanescent waves}, series = {Biosensors and Bioelectronics}, volume = {28}, journal = {Biosensors and Bioelectronics}, number = {1}, issn = {0956-5663}, doi = {10.1016/j.bios.2011.07.030}, pages = {263 -- 269}, abstract = {SPR based biosensors register binding of analytes to the surface with immobilized receptors by measuring changes of the refractive index near this surface. An important task in the improvement of this measurement technology is a separation of signals, corresponding to the changes in the chemosensitive layer, from undesired contributions of bulk phase, for example, due to fluctuations of temperature, concentrations of solutes, pressure. The wavelength of the incident light influences strongly the penetration depth of the corresponding evanescent wave. This dependence was exploited here for compensation of the contribution of the bulk refractive index. It was performed using differential SPR measurements at two wavelengths with differing penetration depths. Theoretical analysis and numerical optimization of the suggested approach, named a Penetration Difference Self-Referencing SPR (PDSR-SPR), were performed. Experimental test was performed using 658 and 980 nm laser diodes. Over 20 times suppression of variations of bulk refractive index with magnitude up to 1000 μRIU was observed. Finally, PDSR-SPR approach was applied for monitoring of antibodies binding to the immobilized antigens.}, language = {en} } @misc{LangeMirsky, author = {Lange, Ulrich and Mirsky, Vladimir M.}, title = {Integrated electrochemical transistor as a fast recoverable gas sensor}, series = {Analytica Chimica Acta}, volume = {687}, journal = {Analytica Chimica Acta}, number = {1}, issn = {0003-2670}, doi = {10.1016/j.aca.2010.12.008}, pages = {7 -- 11}, abstract = {A new design of conductometric chemical sensors based on conducting polymers as chemosensitive elements was suggested. The sensor includes six electrodes. Four inner electrodes coated by chemosensitive polymer are used for simultaneous two- and four-point resistance measurements thus providing information on the bulk polymer resistance and on the resistance of the polymer/electrode contacts. Two outer electrodes wired to inner electrodes by polymeric electrolyte are used for electrical control of redox state of the chemosensitive polymer. The outer electrodes are connected to potentiostat as reference and counter electrodes. It allows us to control redox state of the inner (working) electrodes. This new measurement configuration, resembling chemosensitive electrochemical transistors, provides an internal test of the sensor integrity and an electrically driven sensor regeneration. It was tested as a sensor for the detection of nitrogen dioxide. Polythiophene or polyaniline was used as receptors. Cyclic voltammograms of these polymers on the sensor surface measured in air atmosphere were very similar to that measured in aqueous electrolyte. A control of conductivity of these chemosensitive polymers by electrical potential applied vs. incorporated reference electrode was demonstrated. This effect was used for the regeneration of the chemosensitive material after exposure to nitrogen dioxide: in comparison to usual chemiresistors displaying an irreversible behavior in such test even in the time scale of hours, a completely reversible sensor regeneration within few minutes was observed.}, language = {en} } @misc{LangeMirsky, author = {Lange, Ulrich and Mirsky, Vladimir M.}, title = {Chemiresistors based on conducting polymers: A review on measurement techniques}, series = {Analytica Chimica Acta}, volume = {687}, journal = {Analytica Chimica Acta}, number = {2}, issn = {0003-2670}, doi = {10.1016/j.aca.2010.11.030}, pages = {105 -- 113}, abstract = {This review covers the development of measurement configurations for chemiresistors based on conducting polymers. The simplest chemiresistors are based on application of a two-electrode technique. Artifacts caused by contact resistance can be overcome by application of a four-electrode technique. Simultaneous application of the two- and four-electrode measurement configurations provides an internal control of sensor integrity. An incorporation of two additional electrodes controlling the redox state of chemosensitive polymers and connecting to the measurement electrodes through liquid or (quasi)solid electrolyte results in a six-electrode technique; an electrically driven regeneration of such sensors allows one to perform fast and completely reversible measurements.}, language = {en} } @misc{LangeHirschMirskyetal., author = {Lange, Ulrich and Hirsch, Thomas and Mirsky, Vladimir M. and Wolfbeis, Otto S.}, title = {Hydrogen sensor based on graphene - palladium nanocomposite}, series = {Electrochimica Acta}, volume = {56}, journal = {Electrochimica Acta}, number = {10}, issn = {0013-4686}, doi = {10.1016/j.electacta.2010.10.078}, pages = {3707 -- 3712}, abstract = {A composite material was prepared from graphene and palladium nanoparticles (PdNP) by layer-by-layer deposition on gold electrodes. The material was characterized by absorption spectroscopy, scanning electron microscopy, Raman spectroscopy and surface plasmon resonance. Cyclic voltammetry demonstrated the presence of electrocatalytic centers in the palladium decorated graphene. This material can serve as a sensor material for hydrogen at levels from 0.5 to 1\% in synthetic air. Pure graphene is poorly sensitive to hydrogen, but incorporation of PdNPs increases its sensitivity by more than an order of magnitude. The effects of hydrogen, nitrogen dioxide and humidity were studied. Sensor regeneration is accelerated in humid air. The sensitivity of the nanocomposite depends on the number of bilayers of graphene-PdNPs.}, language = {en} } @misc{LangeMirsky, author = {Lange, Ulrich and Mirsky, Vladimir M.}, title = {Chemosensitive nanocomposite for conductometric detection of hydrazine and NADH}, series = {Electrochimica Acta}, volume = {56}, journal = {Electrochimica Acta}, number = {10}, issn = {0013-4686}, doi = {10.1016/j.electacta.2010.08.092}, pages = {3679 -- 3684}, abstract = {A new chemosensitive material based on palladium nanoparticles and PEDOT-PSS is described. The composite was characterized by transmission electron microscopy, cyclic voltammetry and in situ resistance measurements. The material was applied for conductometric detection of hydrazine and NADH. Upon exposure to these analytes PEDOT is reduced leading to an increase in its conductance. This process is catalyzed by palladium. A model for description of the potential dependence of polymer conductivity was suggested, tested and applied for the development of new calibration procedure of chemiresistors based on electroactive polymers.}, language = {en} } @misc{NizamovScherbahnMirsky, author = {Nizamov, Shavkat and Scherbahn, Vitali and Mirsky, Vladimir M.}, title = {Detection and quantification of single engineered nanoparticles in complex samples using template matching in wide-field surface plasmon microscopy}, series = {Analytical Chemistry}, volume = {88}, journal = {Analytical Chemistry}, number = {20}, issn = {1520-6882}, doi = {10.1021/acs.analchem.6b02878}, pages = {10206 -- 10214}, abstract = {An ultrasensitive analytical method for direct detection of single nanoparticles in complex environment is described. The method relies on the wide-field surface plasmon microscopy (SPM). The suppression of matrix effects is achieved by image analysis based on the template matching. First, characteristic SPM images of nanoparticles are collected in aqueous suspensions. Then the detection of nanoparticles in complex environment is performed using template matching. Quantification and characterization of nanoparticles size was demonstrated at subppb level (∼ 100 pg/mL) in such complex media as wines, fruit juices, or cosmetic formulation. Visualization of the nanoparticles is performed in real time. The method does not require any sample pretreatment. If the minimally acceptable adsorption rate is defined as one nanoparticle to the whole sensor surface per few seconds, the working range of the method is ∼ 10 6 to 10 10 nanoparticles per mL.}, language = {en} } @incollection{YatsimirskyMirsky, author = {Yatsimirsky, Anatoly K. and Mirsky, Vladimir M.}, title = {Quantitative affinity data on selected artificial receptors}, series = {Articicial receptors for chemical sensors}, booktitle = {Articicial receptors for chemical sensors}, editor = {Mirsky, Vladimir M. and Yatsimirsky, Anatoly K.}, publisher = {Wiley VCH Verlag}, address = {Weinheim}, isbn = {978-3-527-32357-9}, doi = {10.1002/9783527632480}, pages = {441 -- 462}, language = {en} } @incollection{Mirsky, author = {Mirsky, Vladimir M.}, title = {Quantitative characterization of affinity properties of immobilized receptors}, series = {Artificial Receptors for Chemical Sensors}, booktitle = {Artificial Receptors for Chemical Sensors}, editor = {Mirsky, Vladimir M. and Yatsimirsky, Anatoly K.}, publisher = {Wiley-VCH Verlag}, address = {Weinheim}, isbn = {978-3-527-32357-9}, doi = {10.1002/9783527632480.ch1}, pages = {1 -- 15}, language = {en} } @incollection{LangeRoznyatovskayaHaoetal., author = {Lange, Ulrich and Roznyatovskaya, Nataliya V. and Hao, Qingli and Mirsky, Vladimir M.}, title = {Conducting polymers as artificial receptors in chemical sensors}, series = {Artificial receptors for chemical sensors}, booktitle = {Artificial receptors for chemical sensors}, editor = {Mirsky, Vladimir M. and Yatsimirsky, Anatoly K.}, publisher = {Wiley-VCH}, address = {Weinheim}, isbn = {978-3-527-32357-9}, doi = {10.1002/9783527632480.ch12}, pages = {363 -- 392}, language = {en} } @misc{IvanovLangeTsakovaetal., author = {Ivanov, Svetlozar D. and Lange, Ulrich and Tsakova, Vessela T. and Mirsky, Vladimir M.}, title = {Electrocatalytically active nanocomposite from palladium nanoparticles and polyaniline: Oxidation of hydrazine}, series = {Sensors and Actuators B: Chemical}, volume = {150}, journal = {Sensors and Actuators B: Chemical}, number = {1}, issn = {0925-4005}, doi = {10.1016/j.snb.2010.07.004}, pages = {271 -- 278}, abstract = {The layer by layer (LbL) adsorption technique was used to deposit a new electrocatalytic material consisting of palladium nanoparticles (Pd NPs) and polyaniline (PANI). As far as PANI adsorption did not affect the reactivity of the Pd NPs attached in the former adsorption step, the LbL technique offered the way of increasing the reactive Pd surface within a three-dimensional nanocomposite structure. In situ conductance measurements have shown that depending on the concentration of the PANI solution, used for the LbL adsorption, composites with either PANI-like (dependent on potential and pH) or metal-like (non-dependent on potential and pH) conductive behaviour can be obtained. Metal-like Pd NPs-PANI nanocomposites were studied as electrocatalytic materials for hydrazine oxidation. A linear concentration dependence of the voltammetric peak currents was observed in the 40-800 μM hydrazine concentration range, the sensitivity increasing with the amount of adsorbed Pd NPs. Amperometric measurements showed linear response in the 10-300 μM range with sensitivity 0.5 μA/μmol cm-2 and a theoretical detection limit estimated to be 0.06 μM.}, language = {en} } @misc{FassoldFalkAndersetal., author = {Fassold, Alexander and Falk, Werner and Anders, Sven and Hirsch, Thomas and Mirsky, Vladimir M. and Straub, Rainer H.}, title = {Soluble neuropilin-2, a nerve repellent receptor, is increased in rheumatoid arthritis synovium and aggravates sympathetic fiber repulsion and arthritis}, series = {Arthritis and Rheumatology}, volume = {60}, journal = {Arthritis and Rheumatology}, number = {10}, issn = {2326-5205}, doi = {10.1002/art.24860}, pages = {2892 -- 2901}, abstract = {In inflammatory lesions, sympathetic nerve fibers disappear soon after the start of inflammation. We identified sympathetic nerve repellents as possible causal agents in rheumatoid arthritis (RA). On nerve terminals, repellent factors bind to neuropilin-2 and its coreceptor. The aim of this study was to investigate the role of neuropilin-2 in the synovial tissue of patients with RA and patients with osteoarthritis (OA) and in experimental arthritis. The density of neuropilin-2-positive fibers and cells positive for semaphorin 3F (a sympathetic repellent) was investigated using immunofluorescence staining. Enzyme-linked immunosorbent assay was used to detect soluble neuropilin-2 in body fluids from patients with RA and patients with OA. An axon outgrowth assay and a neuropilin-2 Fc fusion construct (neuropilin-2Fc) were used to investigate semaphorin 3F-induced sympathetic nerve repulsion. In an animal model of type II collagen-induced arthritis, soluble neuropilin-2Fc was studied in vivo. The synovial density of neuropilin-2-positive sympathetic nerve fibers was lower in RA than in OA, but the density of cells positive for semaphorin 3F was similar. In synovial fluid, the level of soluble neuropilin-2 was markedly higher in RA compared with OA. Mouse sympathetic ganglia served as an excellent model with which to study semaphorin 3F-induced nerve fiber repulsion. Neuropilin-2 and its coreceptor were present on sympathetic neurons, and semaphorin 3F bound to neuropilin-2Fc (binding constant 96 nmoles/liter). Semaphorin 3F dose-dependently increased sympathetic nerve fiber repulsion (at a 50\% maximum response concentration of 160-210 nmoles/liter). In contrast to our expectations, soluble neuropilin-2Fc did not inhibit repulsion but increased the repellent effect of semaphorin 3F. In experimental arthritis, therapy with neuropilin-2Fc aggravated arthritis. Soluble neuropilin-2 has no antirepellent activity but aggravates sympathetic nerve fiber repulsion and arthritis. Increased shedding of neuropilin-2 is probably an unfavorable sign in RA.}, language = {en} } @misc{ShishkovBogachevaDolgovetal., author = {Shishkov, Alexander V. and Bogacheva, Elena Nikolaevna and Dolgov, Alexey A. and Chulikov, Alexey L. and Knyazev, Denis G. and Fedorova, Natalia V. and Ksenofontov, Alexander L. and Kordyukova, Larisa V. and Lukashina, Elena V. and Mirsky, Vladimir M. and Baratova, Lyudmila A.}, title = {The in situ structural characterization of the influenza A virus matrix M1 protein within a virion}, series = {Protein and Peptide Letters}, volume = {16}, journal = {Protein and Peptide Letters}, number = {11}, issn = {0929-8665}, doi = {10.2174/092986609789353682}, pages = {1407 -- 1413}, abstract = {The first attempt has been made to suggest a model of influenza A virus matrix M1 protein spatial structure and molecule orientation within a virion on the basis of tritium planigraphy data and theoretical prediction results. Limited in situ proteolysis of the intact virions with bromelain and surface plasmon resonance spectroscopy study of the M1 protein interaction with lipid coated surfaces were used for independent confirmation of the proposed model.}, language = {en} } @incollection{LangeMirsky, author = {Lange, Ulrich and Mirsky, Vladimir M.}, title = {Potential and pH-dependencies of bulk and contact resistances of conducting polymers}, series = {Electroactive polymers: Materials and devices, Vol. 3}, booktitle = {Electroactive polymers: Materials and devices, Vol. 3}, editor = {Hashmi, S. A. and Chandra, A.}, publisher = {Macmillan Publisher}, address = {New Delhi}, pages = {37 -- 46}, language = {en} } @misc{WolpertWolfbeisMirsky, author = {Wolpert, Benjamin and Wolfbeis, Otto S. and Mirsky, Vladimir M.}, title = {Gas sensing properties of electrically conductive Cu(I) compounds at elevated temperatures}, series = {Sensors and Actuators B: Chemical}, volume = {142}, journal = {Sensors and Actuators B: Chemical}, number = {2}, issn = {0925-4005}, doi = {10.1016/j.snb.2009.04.042}, pages = {446 -- 450}, abstract = {Changes in the electrical conductance at 240 °C of a number of copper(I) compounds including CuI, CuBr, Cu2Te, Cu12Sb4S13, (CuI)2P14, and of NiO were measured following the exposure to the gases ammonia, nitrogen dioxide, nitrous oxide, carbon monoxide, carbon dioxide, and propane. Analytical sensitivity and kinetic parameters were compared quantitatively. The high diversity of sensitivity patterns makes these compounds promising candidates for use in sensor arrays. Principal component analysis of the data obtained with a virtual sensor array enabled highly selective sensing of five of the six analytes studied using only two principle components. Activation energies of the charge transport in the materials were determined.}, language = {en} } @misc{IvanovKurniawanTsakovaetal., author = {Ivanov, Svetlozar D. and Kurniawan, Fredy and Tsakova, Vessela T. and Mirsky, Vladimir M.}, title = {Automated layer-by-layer deposition of polyelectrolytes in flow mode}, series = {Macromolecular Materials and Engineering}, volume = {294}, journal = {Macromolecular Materials and Engineering}, number = {6-7}, issn = {1439-2054}, doi = {10.1002/mame.200800376}, pages = {441 -- 444}, abstract = {Multilayer structures of conducting polymers were fabricated by a simply automated approach in flow mode. Polyaniline and poly(styrene sulfonate) were used as a model system, allowing a fast electrochemical and spectroscopic determination of the amount of deposited material. The technology was applied for layer-by-layer deposition of up to 100 bilayers. The results demonstrate a well reproducible and almost constant amount of the adsorbed polymer at each deposition cycle. The method can be applied for deposition of other conducting or non-conducting polymers, biological macromolecules and composites of polyelectrolytes and nanoparticles.}, language = {en} }