@misc{WhaleSpiegelaereTrypsteenetal., author = {Whale, Alexandra S. and Spiegelaere, Ward De and Trypsteen, W. and Nour, Afif Abdel and Bae, Young-Kyung and Benes, Vladimir and Cleveland, Megan and Devonshire, Alison S. and Dong, Lianhua and Drandi, Daniela and Foy, Carole A. and Garson, Jeremy A. and He, Hua-Jun and Hellemans, Jan and Kubista, Mikael and Lievens, Antoon and Makrigiogos, Mike G. and M{\"u}ller, Reinhold D. and Nolan, Tania and O'Sullivan, Denise M. and Pfaffl, Michael W. and R{\"o}diger, Stefan and Romsos, Erica L. and Shipley, Gregory L. and Taly, Valerie and Untergasser, Andreas and Wittwer, Carl T. and Bustin, Stephen A. and Vandesompele, Jo and Huggett, Jim F.}, title = {The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020}, series = {Clinical Chemistry}, volume = {66}, journal = {Clinical Chemistry}, number = {8}, issn = {0009-9147}, doi = {10.1093/clinchem/hvaa125}, pages = {1012 -- 1029}, language = {en} } @misc{BustinRuijtervandenHoffetal., author = {Bustin, Stephen A and Ruijter, Jan M and van den Hoff, Maurice J B and Kubista, Mikael and Pfaffl, Michael W and Shipley, Gregory L and Tran, Nham and R{\"o}diger, Stefan and Untergasser, Andreas and Mueller, Reinhold and Nolan, Tania and Milavec, Mojca and Burns, Malcolm J and Huggett, Jim F and Vandesompele, Jo and Wittwer, Carl T}, title = {MIQE 2.0 : revision of the minimum information for publication of quantitative real-time PCR experiments guidelines}, series = {Clinical chemistry}, volume = {71}, journal = {Clinical chemistry}, number = {6}, publisher = {Oxford University Press (OUP)}, address = {Washington, DC ; Oxford}, issn = {0009-9147}, doi = {10.1093/clinchem/hvaf043}, pages = {634 -- 651}, abstract = {Background In 2009, the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines established standards for the design, execution, and reporting of quantitative PCR (qPCR) in research. The expansion of qPCR into numerous new domains has driven the development of new reagents, methods, consumables, and instruments, requiring revisions to best practices that are tailored to the evolving complexities of contemporary qPCR applications. Content Transparent, clear, and comprehensive description and reporting of all experimental details are necessary to ensure the repeatability and reproducibility of qPCR results. These revised MIQE guidelines reflect recent advances in qPCR technology, offering clear recommendations for sample handling, assay design, and validation, along with guidance on qPCR data analysis. Instrument manufacturers are encouraged to enable the export of raw data to facilitate thorough analyses and re-evaluation by manuscript reviewers and interested researchers. The guidelines emphasize that quantification cycle (Cq) values should be converted into efficiency-corrected target quantities and reported with prediction intervals, along with detection limits and dynamic ranges for each target, based on the chosen quantification method. Additionally, best practices for normalization and quality control are outlined and reporting requirements have been clarified and streamlined. The aim is to encourage researchers to provide all necessary information without undue burden, thereby promoting more rigorous and reproducible qPCR research. Summary Building on the collaborative efforts of an international team of researchers, we present updates, simplifications, and new recommendations to the original MIQE guidelines, designed to maintain their relevance and applicability in the context of emerging technologies and evolving qPCR applications.}, language = {en} }