@misc{RehacekNovotnyTvarozeketal., author = {Rehacek, Vlastimil and Novotny, Ivan and Tvarozek, Vladimir and Riepl, Michael and Hirsch, T. and Mass, Markus and Schweiss, R. and Mirsky, Vladimir M. and Wolfbeis, Otto S.}, title = {Capacitive detection in thin film chemical sensors and biosensors}, series = {Advanced Semiconductor Devices and Microsystems}, journal = {Advanced Semiconductor Devices and Microsystems}, editor = {Breza, Jura and Donoval, Daniel}, publisher = {IEEE}, address = {Piscataway, NJ}, isbn = {0-7803-4909-1}, pages = {255 -- 258}, abstract = {Thin film electrode chips on silicon substrate have been realized and utilized in development of different chemo- and biosensors. These affinity sensors are based on capacitive transducing and their general structure is thin film Au/alkanethiol/receptor. Several applications of thin film gold electrodes in capacitive sensors are presented.}, language = {en} } @inproceedings{MirskyRieplMassetal., author = {Mirsky, Vladimir M. and Riepl, Michael and Mass, Markus and Hirsch, T. and Schweiss, R. and Wolfbeis, Otto S.}, title = {Capacitive detection of analyte binding in thin film chemo- and biosensors}, series = {Solid State Chemical and Biochemical Sensors}, booktitle = {Solid State Chemical and Biochemical Sensors}, editor = {Vincenzini, Pietro and Dori, L.}, publisher = {Techna}, address = {Faenza}, isbn = {88-86538-27-8}, pages = {441 -- 448}, language = {en} } @misc{MirskyMassKrauseetal., author = {Mirsky, Vladimir M. and Mass, Markus and Krause, Christian and Wolfbeis, Otto S.}, title = {Capacitive approach to determine phospholipase A2 activity towards artificial and natural substrates}, series = {Analytical Chemistry}, volume = {70}, journal = {Analytical Chemistry}, number = {17}, issn = {0003-2700}, doi = {10.1021/ac980102w}, pages = {3674 -- 3678}, abstract = {A capacitive approach has been employed to develop a novel method to determine phospholipase activity. The sensing electrodes have a structure like Au/S(CH₂)₁₇CH₃/substrate/electrolyte. Hydrolysis of the substrate, mediated by phospholipase A₂, leads to the formation of water-soluble products from the insoluble substrate. This results in desorption of these products into aqueous phase and corresponding increase of the electrode capacitance. The requirement of high water solubility of the reaction products can be achieved in two ways. In the first, short-chain phospholipids are used as the substrate, in which case, water-soluble products are formed and no additional reagents are required to promote desorption of these products. The sensors prepared by this strategy provide sensitive qualitative detection of phospholipases. The second way is based on the use of a water-soluble acceptor (for example, β-cyclodextrin) to solubilize the products of hydrolysis. It allows semiquantitative detection of phospholipase activity toward long-chain natural substrates. The reaction kinetics for this case was found to be monoexponential and linearly dependent on the phospholipase concentration. The detection limit of this method, as tested with phospholipase A₂ from bee venom and soy bean lecithin as the substrate, is ∼0.5 ng/mL (500 μunits/mL).}, language = {en} }