@misc{SowaMurugaiyanConradetal., author = {Sowa, Mandy and Murugaiyan, Jayaseelan and Conrad, Karsten and Laass, Martin W. and Bogdanos, Dimitrios Petrou and Papp, Maria and R{\"o}diger, Stefan and Roggenbuck, Dirk and Schierack, Peter}, title = {A novel neutrophil autoantigenic target in inflammatory bowel disease}, language = {en} } @misc{GrossmannRoeberHiemannetal., author = {Großmann, Kai and R{\"o}ber, Nadja and Hiemann, Rico and R{\"o}diger, Stefan and Schierack, Peter and Reinhold, Dirk and Laass, Martin W. and Conrad, Karsten and Roggenbuck, Dirk}, title = {Simultaneous detection of celiac disease-specific IgA antibodies and total IgA}, series = {Autoimmunity Highlights}, volume = {7}, journal = {Autoimmunity Highlights}, number = {2}, issn = {2038-0305}, doi = {10.1007/s13317-016-0073-2}, pages = {10}, language = {en} } @misc{DeutschmannSowaMurugaiyanetal., author = {Deutschmann, Claudia and Sowa, Mandy and Murugaiyan, Jayaseelan and Roessler, Uwe and R{\"o}ber, Nadja and Conrad, Karsten and Laass, Martin W. and Bogdanos, Dimitrios Petrou and Sipeki, Nora and Papp, Maria and R{\"o}diger, Stefan and Roggenbuck, Dirk and Schierack, Peter}, title = {Identification of Chitinase-3-Like Protein 1 as a Novel Neutrophil Antigenic Target in Crohn's Disease}, series = {Journal of Crohn's and Colitis}, volume = {13}, journal = {Journal of Crohn's and Colitis}, number = {7}, issn = {1876-4479}, doi = {10.1093/ecco-jcc/jjz012}, pages = {894 -- 904}, abstract = {Background and Aims There is an increasing incidence of inflammatory bowel disease [IBD]. Autoimmune responses are involved in the pathophysiology of IBD, but their underlying pathways and target antigens have not yet been fully elucidated. Methods Autoantigenic targets in IBD were identified after separation of whole cell proteins isolated from neutrophils using two-dimensional electrophoresis and matrix assisted laser desorption ionization - time of flight mass spectrometry-based protein identification of the spots that displayed Western blotting signals with anti-neutrophil cytoplasmic antibody-positive sera. The prevalence of IgG, IgA and secretory IgA [sIgA] to chitinase 3-like protein 1 [CHI3L1] was analysed by enzyme-linked immunosorbent assays using recombinant CHI3L1 in 110 patients with Crohn's disease [CD], 95 with ulcerative colitis [UC], 126 with coeliac disease [CeD] and 86 healthy controls [HCs]. Results The 18-glycosylhydrolase family member CHI3L1 was identified as a neutrophil autoantigenic target. CD patients displayed significantly higher levels of IgG to CHI3L1 than patients with UC and CeD (p < 0.0001, respectively). IgA and sIgA to CHI3L1 was significantly higher in CD than in UC, CeD and HCs [p < 0.0001, respectively]. IgA and sIgA to CHI3L1 demonstrated the highest prevalence in CD [25.5\%, 28/110; and 41.8\%\%, 46/110] compared to HCs [2.3\%, 2/86; and 4.7\%\%, 4/86; p = 0.0015 and p < 0.0001] and are associated with a more complicated progression of CD. Conclusion CHI3L1 is a novel neutrophil autoantigenic target in CD. IgA and sIgA to CHI3L1 may serve as novel markers for CD and may facilitate the serological diagnosis of IBD.}, language = {en} } @misc{SowaKolendaBaumgartetal., author = {Sowa, Mandy and Kolenda, Rafał and Baumgart, Daniel C. and Pratschke, Johann and Papp, Maria and Tornai, Tamas and Suchanski, Jaroslaw and Bogdanos, Dimitrios Petrou and Mytilinaiou, Maria G. and Hammermann, Jutta and Laass, Martin W. and Conrad, Karsten and Schramm, Christoph and Franke, Andre and Roggenbuck, Dirk and Schierack, Peter}, title = {Mucosal Autoimmunity to Cell-Bound GP2 Isoforms Is a Sensitive Marker in PSC and Associated With the Clinical Phenotype}, series = {Frontiers in Immunology}, volume = {9}, journal = {Frontiers in Immunology}, number = {1959}, issn = {1664-3224}, doi = {10.3389/fimmu.2018.01959}, language = {en} } @misc{RoggenbuckReinholdSchieracketal., author = {Roggenbuck, Dirk and Reinhold, Dirk and Schierack, Peter and Bogdanos, Dimitrios Petrou and Conrad, Karsten and Laass, Martin W.}, title = {Crohn's disease specific pancreatic antibodies: clinical and pathophysiological challenges}, series = {Clinical Chemistry and Laboratory Medicine}, volume = {52}, journal = {Clinical Chemistry and Laboratory Medicine}, number = {4}, issn = {1437-4331}, doi = {10.1515/cclm-2013-0801}, pages = {494}, language = {en} } @misc{RoggenbuckReinholdBaumgartetal., author = {Roggenbuck, Dirk and Reinhold, Dirk and Baumgart, Daniel C. and Schierack, Peter and Conrad, Karsten and Laass, Martin W.}, title = {Autoimmunity in Crohn's Disease-A Putative Stratification Factor of the Clinical Phenotype}, series = {Advances in Clinical Chemistry}, volume = {77}, journal = {Advances in Clinical Chemistry}, issn = {0065-2423}, doi = {10.1016/bs.acc.2016.06.002}, pages = {77 -- 101}, language = {en} } @misc{LopensWunschMilkiewiczetal., author = {Lopens, Steffi and Wunsch, Ewa and Milkiewicz, Malgorzata and R{\"o}ber, Nadja and Zarske, Grit and Nasser, Abdullah and Conrad, Karsten and Laass, Martin W. and R{\"o}diger, Stefan and Krawczyk, Marcin and Roggenbuck, Dirk and Milkiewicz, Piotr}, title = {PR3-ANCAs Detected by Third-Generation ELISA Predicts Severe Disease and Poor Survival in Primary Sclerosing Cholangitis}, series = {Diagnostics}, volume = {12}, journal = {Diagnostics}, number = {11}, issn = {2075-4418}, doi = {10.3390/diagnostics12112682}, abstract = {A highly sensitive detection of anti-neutrophil cytoplasmic antibodies to serine proteinase-3 (PR3-ANCAs) aids in the serological diagnosis of autoimmune liver disorders and the prediction of severity in primary sclerosing cholangitis (PSC). Here, we evaluate a novel third-generation ELISA for the detection of PR3-ANCAs. In total, 309 patients with PSC, 51 with primary biliary cholangitis (PBC), and 120 healthy blood donors (BD) were analyzed. For the survival analysis in PSC, the outcome was defined as liver-transplantation-free survival during the follow-up. Positive PR3-ANCA levels were found in 74/309 (24.0\%) of patients with PSC. No BDs and one patient with PBC demonstrated PR3-ANCA positivity. PR3-ANCAs were revealed as independent predictors for a poor PSC outcome (study endpoint: liver transplantation/death, log-rank test, p = 0.02). PR3-ANCA positivity, lower albumin levels, and higher bilirubin concentrations were independent risks of a poor survival (Cox proportional-hazards regression analysis, p < 0.05). The Mayo risk score for PSC was associated with PR3-ANCA positivity (p = 0.01) and the disease severity assessed with a model of end-stage liver disease (MELD) and extended MELD-Na (p < 0.05). PR3-ANCAs detected by a third-generation ELISA are diagnostic and prognostic markers for PSC. Their wider use could help to identify patients who are at-risk of a more severe disease.}, language = {en} }