@misc{KochStraubMangoldetal., author = {Koch, Roland and Straub, Andrea and Mangold, Sylke and Sch{\"o}pke, Ralph and Martienssen, Marion}, title = {Tausalz, Temperatur und chemische P-F{\"a}llung}, series = {Wasserwirtschaft, Wassertechnik : wwt}, journal = {Wasserwirtschaft, Wassertechnik : wwt}, number = {11/12}, issn = {1438-5716}, pages = {41 -- 46}, language = {de} } @misc{LeschikMusolffMartienssenetal., author = {Leschik, Sebastian and Musolff, Andreas and Martienssen, Marion and Krieg, Ronald and Bayer-Raich, Marti and Reinstorf, Frido and Strauch, Gerhard and Schirmer, Mario}, title = {Investigation of sewer exfiltration using integral pumping tests and wastewater indicators}, doi = {10.1016/j.jconhyd.2009.10.001}, language = {de} } @misc{LeschikMusolffKriegetal., author = {Leschik, Sebastian and Musolff, Andreas and Krieg, Ronald and Martienssen, Marion and Bayer-Raich, Marti and Strauch, Gerhard and Schirmer, Mario and Reinstorf, Frido}, title = {Application of integral pumping tests to investigate the influence of a losing stream on groundwater quality}, doi = {10.5194/hess-13-1765-2009}, language = {de} } @misc{SchmidtKalbusMartienssenetal., author = {Schmidt, C. and Kalbus, Edda and Martienssen, Marion and Schirmer, M.}, title = {The influence of heterogeneous groundwater discharge an the timescales of contaminat mass flux from streambed sediments field evidence and long-term predictions}, series = {Hydrology and Earth Systems Sciences Discussion}, volume = {5}, journal = {Hydrology and Earth Systems Sciences Discussion}, number = {2}, pages = {971 -- 1001}, language = {en} } @misc{MartienssenKuklaBalckeetal., author = {Martienssen, Marion and Kukla, S. and Balcke, G. U. and Hasselwander, E. and Schirmer, M.}, title = {Evidence of natural occurring MTBE biodegradation by analysing metabolites and biodegradation products}, language = {en} } @misc{BeckmannGerhardtZittwitzetal., author = {Beckmann, Annika and Gerhardt, Matthias and Zittwitz, Mark and Martienssen, Marion and Krieg, Ronald and Geistlinger, Helmut and Schirmer, Mario}, title = {Das Oxywall-Projekt: Anwendung eines Verfahrens zur Direktgasinjektion von Sauerstoff zur in situ Sanierung von organisch kontaminierten Grundw{\"a}ssern}, series = {Altlastenspektrum}, volume = {16}, journal = {Altlastenspektrum}, number = {8}, issn = {0942-3818}, pages = {153 -- 159}, language = {de} } @misc{MartienssenReichelSchirmer, author = {Martienssen, Marion and Reichel, O. and Schirmer, M.}, title = {Use of surfactants to improve the biological degradability of petroleum hydrocarbons}, language = {en} } @misc{MartienssenBudahnGruendl, author = {Martienssen, Marion and Budahn, G. and Gr{\"u}ndl, Marlies}, title = {Mikroorganismen im Waschprozeß}, series = {UmweltMagazin}, volume = {27}, journal = {UmweltMagazin}, number = {9}, issn = {0173-363X}, pages = {42 -- 43}, language = {de} } @misc{MartienssenSchulzeSimon, author = {Martienssen, Marion and Schulze, R. and Simon, J.}, title = {Capacities and limits of three different technologies for the biological treatment of leachate from solid waste landfill sites}, language = {en} } @misc{MartienssenSimon, author = {Martienssen, Marion and Simon, J.}, title = {Effect of activated carbon on the biological treatment of oil-water emulsions}, language = {en} } @misc{MartienssenSchoeps, author = {Martienssen, Marion and Sch{\"o}ps, R.}, title = {Biological treatment of leachate from solid waste landfill sites - Alterations in the bacterial community during the denitrification process}, language = {en} } @misc{BeckerMartienssenFuhrmannetal., author = {Becker, M. and Martienssen, Marion and Fuhrmann, Bernd and Spohn, U.}, title = {Analysing nitrogen and sulphide elimination from leachate by coulometric continuous flow titration}, language = {en} } @misc{JankKoeserLueckingetal., author = {Jank, M. and K{\"o}ser, H. and L{\"u}cking, F. and Martienssen, Marion and Wittich, S.}, title = {Decolorization and degradation of Erioglaucine (Acid Blue 9) dye in wastewater}, language = {en} } @misc{MartienssenGruendl, author = {Martienssen, Marion and Gr{\"u}ndl, Marlies}, title = {Einsatz tr{\"a}gerfixierter Mikroorganismen zur Eliminierung von PAH und Mineral{\"o}lkohlenwasserstoffen aus kontaminierten B{\"o}den}, series = {Chemie-Ingenieur-Technik}, volume = {71}, journal = {Chemie-Ingenieur-Technik}, number = {6}, issn = {0009-286X}, pages = {624 -- 628}, language = {de} } @misc{Martienssen, author = {Martienssen, Marion}, title = {Simultaneous catalytic detoxification and biodegradation of organic peroxides during the biofilm process}, language = {en} } @misc{MartienssenGruendl, author = {Martienssen, Marion and Gr{\"u}ndl, Marlies}, title = {Immobilized microorganisms for the degradation of PAH and hydrocarbons from contaminated soil}, language = {en} } @misc{RathsackBoellmannMartienssen, author = {Rathsack, Kristina and B{\"o}llmann, J{\"o}rg and Martienssen, Marion}, title = {Comparative Study of Different Methods for Analyzing Denitrifying Bacteria in Fresh Water Ecosystems}, series = {Journal of Water Resource and Protection}, volume = {6}, journal = {Journal of Water Resource and Protection}, number = {6}, issn = {1945-3094}, pages = {609 -- 617}, abstract = {Bacteria capable of denitrification play a significant role in the nitrogen cycle of freshwater ecosystems. By metabolizing nitrogen compounds they e.g. counteract the eutrophication of natural waters. To get detailed insights into the in situ turnover rates of nitrogen a reliable tool of quantification for active microorganisms is essential. In the present investigation, quantification capabilities of a molecular tool (Polymerase Chain Reaction—PCR) and a cultivation based tool (Most probable number—MPN) were investigated and compared. The total bacterial concentration yielded by the molecular PCR approach was up to 6-fold higher compared to the results of the MPN approach. However, the portion of culturable denitrifying bacteria compared to the number of specific gene copies (nirS) was much lower. Depending on the environmental conditions, the difference between the PCR and the MPN approach was up to three orders of magnitude. From lab scale experiments with a pure P. aeroginosa strain it can be concludes, that these differences are not the result of inappropriate culture conditions but rather reflect the portion of so called viable but not culturable bacteria (VBNC). Low nitrate concentrations as found in many fresh water ecosystems induced a significant increase in the portion of non culturable denitrifying bacteria. Referred to the investigation of dynamic populations, the number of metabolic active bacteria is represented by the MPN rather than by the PCR approach.}, language = {en} } @misc{MartienssenReichelKohlweyer, author = {Martienssen, Marion and Reichel, O. and Kohlweyer, U.}, title = {Surface properties of bacteria from different waste water treatment plants}, series = {Acta Biotechnologica}, volume = {21}, journal = {Acta Biotechnologica}, number = {3}, pages = {207 -- 225}, language = {de} } @misc{SchmidtKalbusKriegetal., author = {Schmidt, Christian and Kalbus, Edda and Krieg, Ronald and Bayer-Raich, Marti and Leschik, Sebastian and Reinstorf, Frido and Martienssen, Marion and Schirmer, Mario}, title = {Contaminant mass flow rates between groundwater, strembed sediments and surface water at the regionally contaminated site Bitterfeld}, series = {Grundwasser}, volume = {13}, journal = {Grundwasser}, number = {3}, issn = {1430-483X}, pages = {133 -- 146}, language = {en} } @misc{SchmidtKalbusMartienssenetal., author = {Schmidt, C. and Kalbus, Edda and Martienssen, Marion and Schirmer, Mario}, title = {The influence of heterogeneous groundwater discharge on the timescales of contaminant mass flux from streambed sediments field evidence and long-term predictions}, series = {Hydrology and Earth System Sciences Discussions}, volume = {5}, journal = {Hydrology and Earth System Sciences Discussions}, number = {2}, issn = {1812-2116}, pages = {971 -- 1001}, language = {en} } @misc{MuhondwaMartienssenBurkhardt, author = {Muhondwa, Jacob Paul and Martienssen, Marion and Burkhardt, Marko}, title = {Effect of high biowaste moisturie content on phatogens hygienization potential of in-vessel composting technology during compost production}, series = {International Journal of Current Research}, volume = {7}, journal = {International Journal of Current Research}, number = {4}, issn = {0975-833X}, pages = {15042 -- 15048}, language = {en} } @misc{CobanKuschkKappelmeyeretal., author = {Coban, Oksana and Kuschk, Peter and Kappelmeyer, Uwe and Spott, Oliver and Martienssen, Marion and Jetten, Mike S. M. and Kn{\"o}ller, Kay}, title = {Nitrogen transforming community in a horizontal subsurface-flow constructed wetland}, series = {Water research}, journal = {Water research}, number = {74}, issn = {1879-2448}, doi = {0.1016/j.watres.2015.02.018}, pages = {203 -- 212}, language = {en} } @misc{MuhondwaMartienssenBurkhardt, author = {Muhondwa, Jacob Paul and Martienssen, Marion and Burkhardt, Marko}, title = {Feasibility of Anaerobic Digestion of Potato peels for Biogas as Mitigation of Greenhouse gases Emission Potential}, series = {International journal of environmental research}, volume = {9}, journal = {International journal of environmental research}, number = {2}, issn = {1735-6865}, pages = {481 -- 488}, language = {en} } @misc{BoellmannRathsackMartienssen, author = {B{\"o}llmann, J{\"o}rg and Rathsack, Kristina and Martienssen, Marion}, title = {The precision of bacterial quantification techniques on different kinds of environmental samples and the effect of ultrasonic treatment}, series = {Journal of Microbiological Methods}, volume = {126}, journal = {Journal of Microbiological Methods}, issn = {0167-7012}, doi = {10.1016/j.mimet.2016.05.006}, pages = {42 -- 47}, abstract = {The precision of cell number quantification in environmental samples depends on the complexity of the sample and on the applied technique. We compared fluorescence microscopy after filtration, quantification of gene copies and the cultivation based most probable number technique for their precision. We further analyzed the effect of increasing complexity of the sample material on the precision of the different methods by using pure cultures of Pseudomonas aeruginosa, fresh water samples and sediment slurries with and without ultrasonic treatment for analyses. Microscopy reached the highest precision, which was similar between pure cultures and water samples, but lower for sediment samples due to a higher percentage of cells in clusters and flocks. The PCR based quantification was most precise for pure cultures. Water and sediment samples were similar but less precise, which might be caused by the applied DNA extraction techniques. MPN measurements were equally precise for pure cultures and water samples. For sediment slurries the precision was slightly lower. The applied ultrasonic treatment of the slurries dispersed the cell clusters and flocks, increased the precision of microscopical and MPN measurements and also increased the number of potential colony forming units. However, the culturable cell number decreased by half. For MPN quantification of viable cells in samples with a high proportion of clustered cells we therefore recommend an optimization of ultrasonic treatment and a confirmation by microscopy and cultivation to reach highest possible dispersion of the cells with a minimum of inactivation. As a result of these observations we suggest a correction factor for MPN measurements to consider the effect of sonication on complex samples. The results are most likely applicable to other complex samples such as soil or biofilms.}, language = {en} } @misc{BryantMartienssenBurkhardt, author = {Bryant, Isaac Mbir and Martienssen, Marion and Burkhardt, Marko}, title = {Millennium development goal on sustainable sanitation in Ghana: A reality or a mirage?}, series = {African Journal of science and research}, volume = {5}, journal = {African Journal of science and research}, number = {3}, issn = {2306-5877}, pages = {01 -- 06}, language = {en} } @misc{EngelbrechtFondengcapMartienssenetal., author = {Engelbrecht, Steffen and Fondengcap, Mbengamina Terence and Martienssen, Marion and Rathsack, Kristina}, title = {Highly efficient long-term storage of carrier-bound anammox biomass}, series = {Water Science \& Technology}, volume = {74}, journal = {Water Science \& Technology}, number = {8}, issn = {0273-1223}, doi = {10.2166/wst.2016.364}, pages = {1911 -- 1918}, abstract = {The anammox process is a potential alternative to the conventional nitrogen removal from wastewater. However, due to large generation times of anammox bacteria, the start-up of treatment reactors may be impeded. An efficient storage technique can handle this drawback and may be also suitable for seasonally operated treatment plants like in touristic areas. In the current study, several storage techniques were investigated with respect to its suitability for the preservation of the specific anammox activity after long-term storage. Storing conditions differed in terms of temperature, redox buffer and nutrient supplementation. The specific activity of immobilized anammox bacteria (Candidatus Kuenenia stuttgartiensis) was determined three times during a long-term preservation of 78 days and 106 days, respectively. The highest activity was ensured at a storing temperature of 4 WC, providing nitrate as redox buffer and a nutrient supplement every 23 days. Thus, 91.4\% of the initial anammox activity could be preserved after a storage of 106 days. Superiority of the presented treatment condition was confirmed by a calculated nitrate-ammonium consumption rate close to the optimal ratio of 1.32. This technique provided an economical and simple method suitable for long-term storage of immobilized anammox biomass.}, language = {en} } @misc{KuhnTothGeppertetal., author = {Kuhn, Ramona and T{\´o}th, Elisabeth and Geppert, Helmut and Fischer, Thomas and Martienssen, Marion}, title = {Identification of the Complete Photodegradation Pathway of Ethylenediaminetetra(methylenephosphonic acid) in Aqueous Solution}, series = {Clean - soil, air, water : a journal of sustainability and environmental safety}, volume = {45}, journal = {Clean - soil, air, water : a journal of sustainability and environmental safety}, number = {5}, issn = {1863-0669}, doi = {10.1002/clen.201500774}, pages = {1 -- 8}, abstract = {This study aims at investigating the abiotic degradation pathway of ethylenediaminetetra(methylenephosphonic acid) (EDTMP) simulated applying UV irradiation. The degradation of EDTMP and formation of degradation products was determined using LC-MS and ³¹P-NMR. In the laboratory scale experiments, EDTMP was degraded within 30 min and the degradation products, iminodi(methylenephosphonic acid) (IDMP), ethylaminobis(methylenephosphonic acid) (EABMP), and amino- (methylenephosphonic acid) (AMPA), were simultaneously released. IDMP was the main degradation product of EDTMP. Therefore, we conclude that the initial cleavage of EDTMP is a heterolytically driven process, which starts the degradation process at the intramolecular C-N bond. In contrast, the main product of a possible homolytic C-C cleavage of methylaminobis(methylenephosphonic acid) could not be confirmed with either LC-MS or ³¹P-NMR. Additionally, there was no evidence for a primary attack on the C-P bond. All identified degradation products of EDTMP have been mineralized to carbon dioxide (CO₂). Three additional degradation products (M1, M2, and M3) have been found using the ³¹P-NMR analysis but have not yet been quantified using LC-MS. We assume that the unidentified degradation product M1 is related to m/z 312, M2 to m/z 341, and M3 to m/z 409. Thus we concluded that EDTMP undergoes photochemical conversion to IDMP, the main degradation product. EABMP and AMPA also accumulate, but in smaller amounts. All intermediates are further mineralized to CO₂.}, language = {en} } @misc{KuhnBoellmannKrahletal., author = {Kuhn, Ramona and B{\"o}llmann, J{\"o}rg and Krahl, Kathrin and Bryant, Isaac Mbir and Martienssen, Marion}, title = {Comparison of ten different DNA extraction procedures with respect to their suitability for environmental samples}, series = {Journal of Microbiological Methods}, volume = {143}, journal = {Journal of Microbiological Methods}, issn = {0167-7012}, doi = {10.1016/j.mimet.2017.10.007}, pages = {78 -- 86}, abstract = {DNA extraction for molecular biological applications usually requires target optimized extraction procedures depending on the origin of the samples. For environmental samples, a range of different procedures has been developed. We compared the applicability and efficiency of ten selected DNA extraction methods published in recent literature using four different environmental samples namely: activated sludge from a domestic wastewater treatment plant, river sediment, anaerobic digestion sludge and nitrifying enrichment culture. We assessed the suitability of the extraction procedures based on both DNA yield and quality. DNA quantification was performed by both ultra violet (UV) spectrophotometry and fluorescence spectrophotometry after staining with PicoGreen. In our study, DNA yields based on UV measurement were overestimated in most cases while DNA yields from fluorescence measurements correlated well with the sample load on agarose gels of crude DNA. The quality of the DNA extracts was determined by gel electrophoresis of crude DNA and PCR products from 16S rDNA with the universal primer set 27f/1525r. It was observed that gel electrophoresis of crude DNA was not always suitable to evaluate DNA integrity and purity since interfering background substances (e.g. humic substances) were not visible. Therefore, we strongly recommend examining the DNA quality of both crude DNA and 16S rDNA PCR products by gel electrophoresis when a new extraction method is established. Summarizing, we found four out of ten extraction procedures being applicable to all tested samples without noticeable restrictions. The procedure G (according to the standard method 432_10401 of the Lower Saxony State Office for Consumer Protection and Food Safety) had the broadest application range over procedure J (published by Wilson, 2001). These were followed by procedures F (Singka et al., 2012) and A (Bourrain et al., 1999). All four extraction procedures delivered reliable and reproducible crude DNA and PCR products. From an economical point of view, all procedures tested during this study were cheaper compared to commercial DNA extraction kits.}, language = {en} } @misc{KuhnBoellmannKrahletal., author = {Kuhn, Ramona and B{\"o}llmann, J{\"o}rg and Krahl, Kathrin and Bryant, Isaac Mbir and Martienssen, Marion}, title = {Data on DNA gel sample load, gel electrophoresis, PCR and cost analysis}, series = {Data in Brief}, volume = {Vol. 16}, journal = {Data in Brief}, issn = {2352-3409}, doi = {10.1016/j.dib.2017.11.082}, pages = {732 -- 751}, abstract = {The data presented in this article provide supporting information to the related research article "Comparison of ten different DNA extraction procedures with respect to their suitability for environmental samples" (revised manuscript submitted to J. Microbiol. Methods). In that article, we compared the suitability of ten selected DNA extraction methods based on DNA quality, purity, quantity and applicability to universal PCR. Here we provide the data on the specific DNA gel sample load, all unreported gel images of crude DNA and PCR results, and the complete cost analysis for all tested extraction procedures and in addition two commercial DNA extraction kits for soil and water.}, language = {en} } @misc{KuhnJenschBryantetal., author = {Kuhn, Ramona and Jensch, Robert and Bryant, Isaac Mbir and Fischer, Thomas and Liebsch, Stephan and Martienssen, Marion}, title = {The influence of selected bivalent metal ions on the photolysis of diethylenetriamine penta(methylenephosphonic acid)}, series = {Chemosphere}, journal = {Chemosphere}, number = {210}, issn = {0045-6535}, doi = {10.1016/j.chemosphere.2018.07.033}, pages = {726 -- 733}, abstract = {DTPMP is predominantly utilized as scale inhibitor. We investigated the reaction rates and degradation mechanism of DTPMP with and without addition of Fe²⁺, Mg²⁺ and Ca²⁺ by performing LC/MS and ³¹P-NMR analyses. DTPMP undergoes conversion with and without addition of bivalent metal ions. The initial cleavage of DTPMP is initiated at the C-N bond leading to release of IDMP as its major breakdown product. The release of smaller quantities of EABMP and AMPA confirmed the nucleophilic attack on the DTPMP amines. Oxidation of Fe²⁺ to Fe³⁺ during the initial 30 min indicated an intramolecular electron transfer changing the electron density distribution at the nitrogen centre, which increased the radical attack during UV irradiation. Independent of the fact that Fe acted as catalyst and Mg²⁺ and Ca²⁺ acted as reactants, we found no significant differences in their degradation mechanisms. However, the reaction rates were strongly affected by the addition of the bivalent metal ions as Fe²⁺ accelerated most DTPMP degradation followed by Mg²⁺ and Ca²⁺. The UV treatment without metal ion addition was four times slower compared with Fe²⁺ addition. We conclude that in environments rich in ferrous iron and/or at reduced redox potential, photolysis of DTPMP will be catalysed by iron and will lead to accumulation of IDMP, EABMP and AMPA and several other none-quantifiable breakdown products.}, language = {en} } @misc{AdonadagaMartienssen, author = {Adonadaga, Melvin-Guy and Martienssen, Marion}, title = {In situ identification of filamentous beacteria from activated studge wastewater treatment plant in Ghana}, series = {Journal of Applied \& Environmental Microbiology}, volume = {3}, journal = {Journal of Applied \& Environmental Microbiology}, number = {3}, issn = {2373-6712}, pages = {75 -- 81}, language = {en} } @misc{AdonadagaMartienssen, author = {Adonadaga, Melvin-Guy and Martienssen, Marion}, title = {Reliability of morphological approach for bulking filamentous bacteria identification in activated sludge}, series = {International Journal of Applied Science and Technology}, volume = {6}, journal = {International Journal of Applied Science and Technology}, number = {2}, issn = {2221-0997}, pages = {30 -- 37}, language = {en} } @misc{AdonadagaAmpaduMartienssen, author = {Adonadaga, Melvin-Guy and Ampadu, Boateng and Martienssen, Marion}, title = {Effect of sludge loading and dissolved oxygen concentration on proliferation of Thiotrix and Eikelboom Types 1851 and 0041 in activated sludge wasterwater treatment plants}, series = {International Journal of Applied Science and Technology}, volume = {6}, journal = {International Journal of Applied Science and Technology}, number = {4}, issn = {2373-6712}, pages = {08 -- 13}, language = {en} } @misc{EngelbrechtMozooniRathsacketal., author = {Engelbrecht, Steffen and Mozooni, Mohammad and Rathsack, Kristina and B{\"o}llmann, J{\"o}rg and Martienssen, Marion}, title = {Effect of increasing salinity to adapted and non-adapted Anammox biofilms}, series = {Environmental Technology}, volume = {40}, journal = {Environmental Technology}, number = {22}, issn = {0959-3330}, doi = {10.1080/09593330.2018.1455748}, pages = {2880 -- 2888}, abstract = {The Anammox process is an efficient low energy alternative for the elimination of nitrogen from wastewater. The process is already in use for side stream applications. However, some industrial wastewaters, e.g. from textile industry are highly saline. This may be a limit for the application of the Anammox process. The aim of this study was to evaluate the effects of different NaCl concentrations on the efficiency of adapted and non-adapted Anammox biofilms. The tested NaCl concentrations ranged from 0 to 50 g NaCl*L⁻¹. Concentrations below 30 g NaCl*L⁻¹did not significantly result in different nitrogen removal rates between adapted and non-adapted bacteria. However, adapted bacteria were significantly more resilient to salt at higher concentrations (40 and 50g NaCl*L⁻¹). The IC50 for adapted and non-adapted Anammox bacteria were 19.99 and 20.30 g NaCl*L⁻¹, respectively. Whereas adapted biomass depletes the nitrogen in ratios of NO-₂/NH+₄ around 1.20 indicating a mainly Anammox-driven consumption of the nitrogen, the ratio increases to 2.21 at 40 g NaCl*L⁻¹ for non-adapted biomass. This indicates an increase of other processes like denitrification. At lower NaCL concentrations up to 10 g NaCl*L⁻¹, a stimulating effect of NaCl to the Anammox process has been observed.}, language = {en} } @misc{BoellmannEngelbrechtMartienssen, author = {B{\"o}llmann, J{\"o}rg and Engelbrecht, Steffen and Martienssen, Marion}, title = {Autofluorescent characteristics of Candidatus Brocadia fulgida and the consequences for FISH and microscopic detection}, series = {Systematic and Applied Microbiology}, volume = {42}, journal = {Systematic and Applied Microbiology}, number = {2}, issn = {1618-0984}, doi = {10.1016/j.syapm.2018.09.002}, pages = {135 -- 144}, abstract = {An enrichment culture of Candidatus Brocadia fulgida was identified by three independent methods: analysis of autofluorescence using different microscope filter blocks and a fluorescence spectrometer, fluorescence in situ hybridization (FISH) with anammox-specific probes and partial sequencing of the 16S rDNA, hydrazine synthase hzsA and hydrazine oxidoreductase hzo. The filter block BV-2A (400-440, 470 LP, Nikon) was suitable for preliminary detection of Ca. B. fulgida. An excitation-emission matrix revealed three pairs of excitation-emission maxima: 288-330 nm, 288-478 nm and 417-478 nm. Several autofluorescent cell clusters could not be stained with DAPI or by FISH, suggesting empty but intact cells (ghost cells) or inhibited permeability. Successful staining of autofluorescent cells with the FISH probes Ban162 and Bfu613, even at higher formamide concentrations, suggested insufficient specificity of Ban162. Under certain conditions, Ca. B. fulgida lost its autofluorescence, which reduced the reliability of autofluorescence for identification and detection. Non-fluorescent Ca. Brocadia cells could not be stained with Ban162, but with Bfu613 at higher formamide concentrations, suggesting a dependency between both parameters. The phylogenetic analysis showed only good taxonomical clustering of the 16S rDNA and hzsA. In conclusion, careful consideration of autofluorescent characteristics is recommended when analysing and presenting FISH observations of Ca. B. fulgida to avoid misinterpretations and misidentifications.}, language = {en} } @misc{KuhnJenschBryantetal., author = {Kuhn, Ramona and Jensch, Robert and Bryant, Isaac Mbir and Fischer, Thomas and Liebsch, Stephan and Martienssen, Marion}, title = {Rapid sample clean-up procedure for aminophosphonate determination by LC/MS analysis}, series = {Talanta}, volume = {208}, journal = {Talanta}, issn = {0039-9140}, doi = {10.1016/j.talanta.2019.120454}, pages = {6}, abstract = {Aminophosphonates are commonly utilised for complexing bivalent ions such as calcium and magnesium. With regard to environmental samples, the analysis of these highly polar compounds is still challenging due to matrix effects and lacking analytical standard methods. We have recently developed a LC/MS method for common aminophosphonates without derivatisation. This LC/MS method delivers precise and accurate measurement for standard samples with very low concentration of cations disturbing the analysis. However, due to matrix effects this LC/MS method requires a sample clean-up being also applicable to natural water or wastewater samples. We developed and optimised a sample clean-up procedure applying strong cation exchange resin Dowex 50WX8. This clean-up allows a single LC/MS analysis of hydroxyethelidene(diphosphonic acid) (HEDP), aminotris(methylenephosphonic acid) (ATMP), ethylenediaminetetra(methyloenephosphonic acid) (EDTMP), diethylenetriaminepenta(methylenephosphonic acid) (DTPMP) and its major intermediates amino(methylphosphonic acid) (AMPA) and iminodi(methylenephosphonic acid) (IDMP). We compared different test conditions with six aminophosphonates in either ultra pure water or tap water. The latter was used to simulate cation concentrations typical for natural waters. To elute all aminophosphonate including AMPA, ammonium acetate addition was necessary. The addition of ammonium acetate was combinable with all tested aminophosphonates and provides high sample quality for LC/MS analysis. For acceptable recovery, the smallest aminophosphonate AMPA required the highest addition of ammonium acetate (1000 mg L-1) during the sample clean-up. Finally, the optimised clean-up procedure was successfully applied to identify and quantify phosphonates from an industrial wastewater sample. The sample clean-up procedure is simple, cheap, rapid and precise and can be further combined with solid phase extraction and more sensitive LC/MS methods.}, language = {en} } @misc{MartienssenBoellmannNixdorfetal., author = {Martienssen, Marion and B{\"o}llmann, J{\"o}rg and Nixdorf, Brigitte and Rathsack, Kristina}, title = {Calculation of hypolimnic denitrification in a dimictic freshwater lake during summer stratification}, series = {Aquatic Microbial Ecology}, volume = {83}, journal = {Aquatic Microbial Ecology}, number = {2}, issn = {0948-3055}, doi = {10.3354/ame01911}, pages = {189 -- 201}, language = {en} } @misc{BryantBurkhardtMartienssen, author = {Bryant, Isaac Mbir and Burkhardt, Marko and Martienssen, Marion}, title = {Comparison of performance of three diferent seeding sludge under three different hyper-thermophilic temperatures}, series = {International Journal of Environmental Engineering}, volume = {10}, journal = {International Journal of Environmental Engineering}, number = {1}, issn = {1756-8471}, doi = {10.1504/IJEE.2019.099979}, pages = {1 -- 16}, language = {en} } @misc{FurusatoBhuyanMartienssenetal., author = {Furusato, Eiichi and Bhuyan, Jayatu and Martienssen, Marion and Nixdorf, Brigitte and Imamoto, Hiroomi}, title = {Critical Depth Model (modified Sverdrup equation) - Design method for bubble circulation}, series = {Journal of JSCE}, volume = {7}, journal = {Journal of JSCE}, number = {1}, issn = {2187-5103}, doi = {10.2208/journalofjsce.7.1_185}, pages = {185 -- 192}, language = {en} } @misc{KuhnJenschBryantetal., author = {Kuhn, Ramona and Jensch, Robert and Bryant, Isaac Mbir and Fischer, Thomas and Liebsch, Stephan and Martienssen, Marion}, title = {Photodegradation of ethylenediaminetetra(methylenephosphonic acid) - The effect of the system configuration}, series = {Journal of Photochemistry \& Photobiology A: Chemistry}, volume = {388}, journal = {Journal of Photochemistry \& Photobiology A: Chemistry}, issn = {1010-6030}, doi = {10.1016/j.jphotochem.2019.112192}, pages = {9}, abstract = {Photodegradation of aminophosphonates such as ethylenediaminetetra(methylenephosphonic acid) (EDTMP) is recently assumed being the major degradation pathway in aquatic environments. Several photolysis studies were reported about EDTMP and possible breakdown products occurring in natural ecosystems. Reliable prediction of environmental photolysis of parent compounds and possible release of breakdown products requires different set-up conditions and varying the parameters influencing the photodegradation. We studied the influence of three different system configurations during UV degradation of EDTMP. These three configurations differed either in geometry and/or treated sample volumes. System 1 was equipped with a direct cooling jacket at the UV lamp. System 2 had the geometry of system 1 but there was no usage of a direct cooling jacket. System 3 was a gas-tight system with a larger sample volume. Using the chemical actinometer potassium ferrioxalate, we determined the highest photon flux for system 3 followed by system 2 and 1. In addition, we performed scavenger experiments with methanol and ascorbic acid in order to prove the dominating radical species. In system 1, the addition of methanol showed almost no effect while the ascorbic acid resulted in a reduction of 57.1\% orthophosphate released. Therefore we conclude that in system 1 the radical-drive degradation of EDTMP is mainly based on superoxide radicals. In system 2 and 3 both radical species, i.e., hydroxyl radicals and superoxide radicals, contribute to the photodegradation of EDTMP. We determined different half-lives for EDTMP for the three different systems configurations. For system 1, the estimated half-life achieved was 14.09 ± 0.15 min. For system 2 and 3, the half-lives were almost similar and averaged 4.75 ± 0.05 min and 5.02 ± 0.20 min, respectively. Contrary to our assumption to also find the highest degradation rate for system 3, we found the highest degradation rate for system configuration 2 as a result of the differences in the construction and geometry of the three systems. Our findings lead us to recommend the three system configuration for different research purposes. Thus, we recommend system 1 for detailed studies on the degradation pathway of the parent compound and their breakdown products. System 2 is recommended as a suitable configuration for kinetic studies of the parent compound. And finally, we recommend the system configuration 3 for complete mass balances. The gas-tight system allows determining all soluble and gaseous compounds.}, language = {en} } @misc{KuhnBryantJenschetal., author = {Kuhn, Ramona and Bryant, Isaac Mbir and Jensch, Robert and Liebsch, Stephan and Martienssen, Marion}, title = {Photolysis of hexamethylenediaminetetra(methylenephosphonic acid) (HDTMP) using manganese and hydrogen peroxide}, series = {Emerging Contaminants}, volume = {2020}, journal = {Emerging Contaminants}, number = {6}, doi = {10.1016/j.emcon.2019.11.003}, pages = {10 -- 19}, abstract = {Aminophosphonates such as hexamethylenediaminetreta(methylene phosphonic acid) (HDTMP) are categorised as persistent substances. They are commonly used as scale inhibitors in cooling water systems and desalination processes. After utilisation, they are often discharged into aquatic environment without pre-treatment. Advanced oxidation processes (AOP) are promising pre-treatments for industrial wastewater treatments. We investigated the photodegradation of HDTMP with or without addition of manganese (Mn2þ) and/or H2O2. Similar to results of our former photodegradation studies, we found that HDTMP also undergoes conversion with or without additives during the ultra violet (UV) irradiation. The reaction rate was most affected by the addition of H2O2, i.e. the HDTMP degradation was accelerated by a factor 3.85 compared with UV treatment without additives. The addition of Mn2þ accelerated the degradation of HDTMP only by a factor 1.53 compared with the UV treatment without additives. The combined addition of Mn2þ and H2O2 accelerated the HDTMP degradation by a factor 2.81. Interestingly, the initial cleavage is not initiated as expected at the CeN bond but at the CeP bond of the methyl carbon and the phosphorus of the methylenephosphonic acid group of HDTMP. This initial cleavage was independent whether the UV treatment was performed with or without additives. Therefore, we conclude that the degradation mechanism is similar independent of the four tested treatment conditions. We identified amino(methylenephosphonic acid) AMPA, dimethylamino(methylenephosphonic acid) DAMP and iminodi(methylenephosphonic acid) IDMP as the major breakdown products by performing LC/MS analyses. The major mineralisation products were ortho-phosphate, ammonium and carbon dioxide. The mass balances of unknown breakdown products}, language = {en} } @misc{KuhnBryantMartienssen, author = {Kuhn, Ramona and Bryant, Isaac Mbir and Martienssen, Marion}, title = {Supplementary data on rapid sample clean-up procedure for aminophosphonate determination by LC/MS analysis}, series = {MethodsX}, volume = {07}, journal = {MethodsX}, issn = {2215-0161}, doi = {10.1016/j.mex.2020.100933}, pages = {1 -- 11}, abstract = {Minimising matrix effects through high sample purity is of major importance for LC/MS analysis. Here we provide supplementary data and protocols related to the article "Rapid sample clean-up procedure of aminophosphonates for LC/MS analysis"(revised article submitted to Talanta) [1] . It is demonstrated that the tested phosphonates iminodi(methylenephosphonic acid) (IDMP), hydroxyethelidene(diphosphonic acid) (HEDP), aminotris(methylenephosphonic acid) (ATMP), ethylenediaminetetra(methyloenephosphonic acid) (EDTMP) and diethylenetriaminepenta(methylenephosphonic acid) (DTPMP) dissolved in tap water are not detectable by LC/MS without sample clean-up. Only the smallest aminophosphonate amino(methylenephosphonic acid) (AMPA) was detectable but the recovery is decreased drastically. The optimised sample clean-up with cation exchange resin (CER) Dowex 50WX8 is described in detail and illustrated. The protocol is provided. The influence of the incubation time, addition of different ammonium acetate concentrations, different samples pHs and different water qualities is demonstrated and preferred clean- up conditions are recommended. Calibration results of all tested aminophosphonates are validated regarding limit of detection, limit of quantification, lower limit of quantification, absolute and relative process standard deviation. A final recommendation for the best clean-up condition for all six tested aminophosphonates is provided.}, language = {en} } @misc{KuhnVornholtPreussetal., author = {Kuhn, Ramona and Vornholt, Carsten and Preuß, Volker and Bryant, Isaac Mbir and Martienssen, Marion}, title = {Aminophosphonates in Nanofiltration and Reverse Osmosis Permeates}, series = {Membranes}, volume = {11}, journal = {Membranes}, number = {6}, issn = {2077-0375}, doi = {10.3390/membranes11060446}, pages = {1 -- 16}, abstract = {Aminophosphonates such as aminotris(methylenephosphonic acid) (ATMP) are common constituents of antiscalants. In nanofiltration (NF) and reverse osmosis (RO) processes, ATMP prevents inorganic scaling leading to more stable membrane performance. So far, little attention has been paid to the possible permeation of aminophosphonates through NF and RO membranes. We have investigated the permeability of these membrane types for ATMP and its potential metabolites iminodi(methylenephosphonic acid) (IDMP) and amino(methylenephosphonic acid) (AMPA) with two different NF membranes (TS40 and TS80) and one RO membrane (ACM2) and three different water compositions (ultra-pure water, synthetic tap water and local tap water). We found traces of phosphonates in all investigated permeates. The highest phosphonate rejection occurred with local tap water for all three membranes investigated. Filtration experiments with a technical antiscalant formulation containing ATMP indicated similar trends of phosphonate permeability through all three membranes. We assume that the separation mechanisms of the membranes are the results of a very complex relationship between physico-chemical properties such as Donnan exclusion, feed pH, feed ionic strength and feed concentration, as well as solute-solute interactions.}, language = {en} } @misc{BoellmannMartienssen, author = {B{\"o}llmann, J{\"o}rg and Martienssen, Marion}, title = {Comparison of different media for the detection of denitrifying and nitrate reducing bacteria in mesotrophic aquatic environments by the most probable number method}, series = {Journal of Microbiological Methods}, volume = {168}, journal = {Journal of Microbiological Methods}, issn = {1872-8359}, doi = {10.1016/j.mimet.2019.105808}, pages = {10}, abstract = {The cultivation based characterization of microbial communities and the quantification of certain functional bacterial groups is still an essential part of microbiology and microbial ecology. For plate count methods meanwhile low strength media are recommended, since they cover a broader range of different species and result in higher counts compared to established high strength media. For liquid media, as they are used for most probable number (MPN) quantifications, comparisons between high and low strength media are rare. In this study we compare the performance of different high and low strength media for the MPN quantification of nitrate reducing and denitrifying bacteria in two different fresh water environments. We also calculated the cell specific turnover rates of several denitrifying cultures previously enriched in high and low strength media from three different fresh water environments and a waste water treatment plant. For fresh water samples, our results indicate that high strength media detect higher MPN of denitrifying bacteria and in equal MPN of nitrate reducing bacteria compared to low strength media, which is in contrary to plate count techniques. For sediment samples, high and low strength media performed equal. The cell specific turnover rate was independent from the enrichment media and the media of the performance test. The cause of the lower denitrifyer MPN in low strength media remains, however, unclear. The results are important for further MPN quantifications of bacteria in nutrient poor environments and for calculations of nitrogen turnover rates by kinetical models using the number of metabolic active cells as one parameter.}, language = {en} } @misc{BoellmannMartienssen, author = {B{\"o}llmann, J{\"o}rg and Martienssen, Marion}, title = {Impact of pH conditions and the characteristics of two electrodialysis membranes on biofilm development under semi-realistic conditions}, series = {Biofouling}, volume = {37}, journal = {Biofouling}, number = {9-10}, issn = {1029-2454}, doi = {10.1080/08927014.2021.1999424}, pages = {998 -- 1005}, abstract = {The reuse of treated wastewater for irrigation is of increasing importance. The Ecosave farming project developed a new photocatalytic electrodialysis process for desalination and hygienization. However, membrane scaling significantly reduces filtration efficiency. This study investigated biofilm development on anion and cation exchange membranes at a wide pH range in pre-treated wastewater. Epifluorescence microscopic quantification of the biofilm by cell counts and surface coverage together with 16S rDNA gene copy numbers showed stronger biofilm development on the anion exchange membrane (AEM) compared with the cation exchange membrane (CEM) with up to 105 cells mm-2 and 20\% surface coverage after three weeks. As the AEM biofilm developed best in neutral and a slightly alkaline pH, the CEM was colonized preferably at alkaline conditions. Extreme pH conditions strongly inhibited biofilm growth, which might help to minimize the maintenance effort by creating those conditions during the operation of the dialysis cell itself.}, language = {en} } @misc{HertelGibhardtMartienssenetal., author = {Hertel, Robert and Gibhardt, Johannes and Martienssen, Marion and Kuhn, Ramona and Commichau, Fabian M.}, title = {Molecular mechanisms underlying glyphosate resistance in bacteria}, series = {Environmental Microbiology}, volume = {23}, journal = {Environmental Microbiology}, number = {6}, issn = {1462-2920}, doi = {10.1111/1462-2920.15534}, pages = {2891 -- 2905}, abstract = {Glyphosate is a nonselective herbicide that kills weeds and other plants competing with crops. Glyphosate specifically inhibits the 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase, thereby depleting the cell of EPSP serving as a precursor for biosynthesis of aromatic amino acids. Glyphosate is considered to be toxicologically safe for animals and humans. Therefore, it became the most-important herbicide in agriculture. However, its intensive application in agriculture is a serious environmental issue because it may negatively affect the biodiversity. A few years after the discovery of the mode of action of glyphosate, it has been observed that bacteria evolve glyphosate resistance by acquiring mutations in the EPSP synthase gene, rendering the encoded enzyme less sensitive to the herbicide. The identification of glyphosate-resistant EPSP synthase variants paved the way for engineering crops tolerating increased amounts of the herbicide. This review intends to summarize the molecular mechanisms underlying glyphosate resistance in bacteria. Bacteria can evolve glyphosate resistance by (i) reducing glyphosate sensitivity or elevating production of the EPSP synthase, by (ii) degrading or (iii) detoxifying glyphosate and by (iv) decreasing the uptake or increasing the export of the herbicide. The variety of glyphosate resistance mechanisms illustrates the adaptability of bacteria to anthropogenic substances due to genomic alterations.}, language = {en} } @misc{HertelSchoeneMittelstaedtetal., author = {Hertel, Robert and Sch{\"o}ne, Kerstin and Mittelst{\"a}dt, Carolin and Meißner, Janek and Zschoche, Nick and Collignon, Madeline and Kohler, Christian and Friedrich, Ines and Schneider, Dominik and Hoppert, Michael and Kuhn, Ramona and Schwedt, Inge and Scholz, Patricia and Poehlein, Anja and Martienssen, Marion and Ischebeck, Till and Daniel, Rolf and Commichau, Fabian M.}, title = {Characterization of glyphosate-resistant Burkholderia anthina and Burkholderia cenocepacia isolates from a commercial Roundup® solution}, series = {Environmental Microbiology Reports}, volume = {14}, journal = {Environmental Microbiology Reports}, number = {1}, issn = {1758-2229}, doi = {10.1111/1758-2229.13022}, pages = {70 -- 84}, abstract = {Roundup® is the brand name for herbicide solutions containing glyphosate, which specifically inhibits the 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase of the shikimate pathway. The inhibition of the EPSP synthase causes plant death because EPSP is required for biosynthesis of aromatic amino acids. Glyphosate also inhibits the growth of archaea, bacteria, Apicomplexa, algae and fungi possessing an EPSP synthase. Here, we have characterized two glyphosate-resistant bacteria from a Roundup solution. Taxonomic classification revealed that the isolates 1CH1 and 2CH1 are Burkholderia anthina and Burkholderia cenocepacia strains respectively. Both isolates cannot utilize glyphosate as a source of phosphorus and synthesize glyphosate-sensitive EPSP synthase variants. Burkholderia. anthina 1CH1 and B. cenocepacia 2CH1 tolerate high levels of glyphosate because the herbicide is not taken up by the bacteria. Previously, it has been observed that the exposure of soil bacteria to herbicides like glyphosate promotes the development of antibiotic resistances. Antibiotic sensitivity testing revealed that the only the B. cenocepacia 2CH1 isolate showed increased resistance to a variety of antibiotics. Thus, the adaptation of B. anthina 1CH1 and B. cenocepacia 2CH1 to glyphosate did not generally increase the antibiotic resistance of both bacteria. However, our study confirms the genomic adaptability of bacteria belonging to the genus Burkholderia.}, language = {en} } @misc{KuhnJenschFischeretal., author = {Kuhn, Ramona and Jensch, Robert and Fischer, Thomas and Keuler, Klaus and Bryant, Isaac Mbir and Martienssen, Marion}, title = {Sunlight Degradation of the Aminophosphonate Diethylenetriamine Penta-(Methylenephosphonic Acid)}, series = {Solar}, volume = {2}, journal = {Solar}, number = {2}, issn = {2673-9941}, doi = {10.3390/solar2020009}, pages = {141 -- 157}, abstract = {Aminophosphonate diethylenetriamine penta(methylenephosphonic acid) (DTPMP) is a scale inhibitor commonly used in several industries. DTPMP is suspected to cause anthropogenic pollution through discharge into the aquatic environment. DTPMP is assumed to be degraded by sunlight radiation. We recently predicted a preliminary degradation pathway of DTPMP applyingUV treatment. Currently, we have not yet evidenced that DTPMP shows the same degradation pattern with natural sunlight. One major reason leads to the fact that the light spectrum emitted by UV lamps does not completely represent the natural sunlight spectrum, and the emitted UVB and UVA irradiation flux is much higher than for solar light. For that reason, the degradation pattern and kinetics might be different between artificial UV treatment and natural sunlight treatment. Here, we investigated whether DTPMP is degradable under natural sunlight radiation, and whether the degradation mechanisms determined through UV treatment are transferable to sunlight. We investigated five different treatment conditions, i.e., DTPMP degradation in direct or diffuse sunlight, in diffuse sunlight with addition of Ca2+ or Mg2+, and in diffuse sunlight with local TW. Our experiment was carried out from March 2021 to October 2021. We performed LC/MS analyses and measured the release of o-PO4 3 . DTPMP was degraded with all five treatment conditions. The fastest DTPMP degradation occurred in direct and diffuse sunlight without addition of bivalent cations. The addition of Ca2+ and Mg2+ resulted in inhibited degradation. Similar effects occurred for sunlight treatment with local TW. We evidenced different degradation mechanisms for DTPMP depending on the presence of alkaline earth metals as we previously proposed for UV-treated DTPMP. However, both degradation mechanisms of DTPMP belong to the same degradation pathway determined with UV treatment. Therefore, we conclude that DTPMP undergoes a similar degradation pathway in sunlight as compared to UV light.}, language = {en} } @misc{SarpongAmankwaaMartienssenetal., author = {Sarpong, Daniella and Amankwaa, Gordon and Martienssen, Marion and Burkhardt, Marko}, title = {A novel biomethane (BMP) and somposting (CMP) potential framework for determining biogas and composting potential of urban organic waste}, series = {Environmental Technology}, volume = {45(2024)}, journal = {Environmental Technology}, number = {8}, issn = {1479-487X}, doi = {10.1080/09593330.2022.2145241}, pages = {1471 -- 1482}, language = {en} } @misc{OkofoMartienssen, author = {Okofo, Louis Boansi and Martienssen, Marion}, title = {A three-dimensional numerical groundwater flow model to assess the feasibility of managed aquifer recharge in the Tamne River basin of Ghana}, series = {Hydrogeology Journal}, volume = {30}, journal = {Hydrogeology Journal}, number = {4}, issn = {1431-2174}, doi = {10.1007/s10040-022-02492-7}, pages = {1071 -- 1090}, language = {en} }