@misc{ScholzGrossmannKnuetteretal., author = {Scholz, Juliane and Großmann, Kai and Kn{\"u}tter, Ilka and Hiemann, Rico and Sowa, Mandy and R{\"o}ber, Nadja and R{\"o}diger, Stefan and Schierack, Peter and Reinhold, Dirk and Bogdanos, Dimitrios Petrou and Meroni, Pier Luigi and Radice, Antonella and Conrad, Karsten and Roggenbuck, Dirk}, title = {Second generation analysis of antinuclear antibody (ANA) by combination of screening and confirmatory testing}, series = {Clinical Chemistry and Laboratory Medicine}, volume = {53}, journal = {Clinical Chemistry and Laboratory Medicine}, number = {12}, issn = {1437-4331}, doi = {10.1515/cclm-2015-0083}, pages = {1991 -- 2002}, language = {en} } @misc{SowaMurugaiyanConradetal., author = {Sowa, Mandy and Murugaiyan, Jayaseelan and Conrad, Karsten and Laass, Martin W. and Bogdanos, Dimitrios Petrou and Papp, Maria and R{\"o}diger, Stefan and Roggenbuck, Dirk and Schierack, Peter}, title = {A novel neutrophil autoantigenic target in inflammatory bowel disease}, language = {en} } @misc{SowaGrossmannKnuetteretal., author = {Sowa, Mandy and Großmann, Kai and Kn{\"u}tter, Ilka and Hiemann, Rico and R{\"o}ber, Nadja and Anderer, Ursula and Csernok, Elena and Bogdanos, Dimitrios Petrou and Borghi, Maria Orietta and Meroni, Pier Luigi and Schierack, Peter and Reinhold, Dirk and Conrad, Karsten and Roggenbuck, Dirk}, title = {Simultaneous automated screening and confirmatory testing for vasculitis-specific ANCA}, series = {PLoS ONE}, volume = {9}, journal = {PLoS ONE}, number = {9}, issn = {1932-6203}, doi = {10.1371/journal.pone.0107743}, pages = {11}, language = {en} } @misc{SchloerHolzloehnerListeketal., author = {Schl{\"o}r, Anja and Holzl{\"o}hner, Pamela and Listek, Martin and Grieß, Cindy and Butze, Monique and Micheel, Burkhard and Hentschel, Christian and Sowa, Mandy and Roggenbuck, Dirk and Schierack, Peter and F{\"u}ner, Jonas and Schliebs, Erik and Goihl, Alexander and Reinhold, Dirk and Hanack, Katja}, title = {Generation and validation of murine monoclonal and camelid recombinant single domain antibodies specific for human pancreatic glycoprotein 2}, series = {New Biotechnology}, volume = {45}, journal = {New Biotechnology}, issn = {1876-4347}, doi = {10.1016/j.nbt.2018.03.006}, pages = {60 -- 68}, language = {en} } @misc{DeutschmannSowaMurugaiyanetal., author = {Deutschmann, Claudia and Sowa, Mandy and Murugaiyan, Jayaseelan and Roessler, Uwe and R{\"o}ber, Nadja and Conrad, Karsten and Laass, Martin W. and Bogdanos, Dimitrios Petrou and Sipeki, Nora and Papp, Maria and R{\"o}diger, Stefan and Roggenbuck, Dirk and Schierack, Peter}, title = {Identification of Chitinase-3-Like Protein 1 as a Novel Neutrophil Antigenic Target in Crohn's Disease}, series = {Journal of Crohn's and Colitis}, volume = {13}, journal = {Journal of Crohn's and Colitis}, number = {7}, issn = {1876-4479}, doi = {10.1093/ecco-jcc/jjz012}, pages = {894 -- 904}, abstract = {Background and Aims There is an increasing incidence of inflammatory bowel disease [IBD]. Autoimmune responses are involved in the pathophysiology of IBD, but their underlying pathways and target antigens have not yet been fully elucidated. Methods Autoantigenic targets in IBD were identified after separation of whole cell proteins isolated from neutrophils using two-dimensional electrophoresis and matrix assisted laser desorption ionization - time of flight mass spectrometry-based protein identification of the spots that displayed Western blotting signals with anti-neutrophil cytoplasmic antibody-positive sera. The prevalence of IgG, IgA and secretory IgA [sIgA] to chitinase 3-like protein 1 [CHI3L1] was analysed by enzyme-linked immunosorbent assays using recombinant CHI3L1 in 110 patients with Crohn's disease [CD], 95 with ulcerative colitis [UC], 126 with coeliac disease [CeD] and 86 healthy controls [HCs]. Results The 18-glycosylhydrolase family member CHI3L1 was identified as a neutrophil autoantigenic target. CD patients displayed significantly higher levels of IgG to CHI3L1 than patients with UC and CeD (p < 0.0001, respectively). IgA and sIgA to CHI3L1 was significantly higher in CD than in UC, CeD and HCs [p < 0.0001, respectively]. IgA and sIgA to CHI3L1 demonstrated the highest prevalence in CD [25.5\%, 28/110; and 41.8\%\%, 46/110] compared to HCs [2.3\%, 2/86; and 4.7\%\%, 4/86; p = 0.0015 and p < 0.0001] and are associated with a more complicated progression of CD. Conclusion CHI3L1 is a novel neutrophil autoantigenic target in CD. IgA and sIgA to CHI3L1 may serve as novel markers for CD and may facilitate the serological diagnosis of IBD.}, language = {en} } @misc{SowaKolendaBaumgartetal., author = {Sowa, Mandy and Kolenda, Rafał and Baumgart, Daniel C. and Pratschke, Johann and Papp, Maria and Tornai, Tamas and Suchanski, Jaroslaw and Bogdanos, Dimitrios Petrou and Mytilinaiou, Maria G. and Hammermann, Jutta and Laass, Martin W. and Conrad, Karsten and Schramm, Christoph and Franke, Andre and Roggenbuck, Dirk and Schierack, Peter}, title = {Mucosal Autoimmunity to Cell-Bound GP2 Isoforms Is a Sensitive Marker in PSC and Associated With the Clinical Phenotype}, series = {Frontiers in Immunology}, volume = {9}, journal = {Frontiers in Immunology}, number = {1959}, issn = {1664-3224}, doi = {10.3389/fimmu.2018.01959}, language = {en} } @misc{SowaGrossmannScholzetal., author = {Sowa, Mandy and Großmann, Kai and Scholz, Juliane and R{\"o}ber, Nadja and R{\"o}diger, Stefan and Schierack, Peter and Conrad, Karsten and Roggenbuck, Dirk and Hiemann, Rico}, title = {Der CytoBead-Assay - Eine neue M{\"o}glichkeit der multiparametrischen Autoantik{\"o}rperanalytik bei systemischen Autoimmunerkrankungen}, series = {Journal of Laboratory Medicine}, volume = {38}, journal = {Journal of Laboratory Medicine}, number = {6}, doi = {10.1515/labmed-2014-0041}, pages = {309 -- 317}, language = {de} } @misc{SowaTrezziHiemannetal., author = {Sowa, Mandy and Trezzi, Barbara and Hiemann, Rico and Schierack, Peter and Großmann, Kai and Scholz, Juliane and Somma, Valentina and Sinico, Renato Alberto and Roggenbuck, Dirk and Radice, Antonella}, title = {Simultaneous comprehensive multiplex autoantibody analysis for rapidly progressive glomerulonephritis}, series = {Medicine}, volume = {95}, journal = {Medicine}, number = {44}, issn = {0025-7974}, doi = {10.1097/MD.0000000000005225}, pages = {5225}, language = {en} } @misc{SowaHiemannSchieracketal., author = {Sowa, Mandy and Hiemann, Rico and Schierack, Peter and Reinhold, Dirk and Conrad, Karsten and Roggenbuck, Dirk}, title = {Next-Generation Autoantibody Testing by Combination of Screening and Confirmation-the CytoBead® Technology}, series = {Clinical Reviews in Allergy \& Immunology}, volume = {53}, journal = {Clinical Reviews in Allergy \& Immunology}, number = {1}, issn = {1559-0267}, doi = {10.1007/s12016-016-8574-3}, pages = {87 -- 104}, language = {en} } @misc{SowaReddigSchieracketal., author = {Sowa, Mandy and Reddig, Annika and Schierack, Peter and Reinhold, Dirk and Roggenbuck, Dirk}, title = {Phosphorylated histone 2AX foci determination in capillary blood mononuclear cells}, series = {Journal of Laboratory and Precision Medicine}, volume = {3}, journal = {Journal of Laboratory and Precision Medicine}, issn = {2519-9005}, doi = {10.21037/jlpm.2018.04.02}, pages = {6}, language = {en} } @misc{LiedtkeRoseHiemannetal., author = {Liedtke, Victoria and Rose, Laura and Hiemann, Rico and Nasser, Abdullah and R{\"o}diger, Stefan and Bonaventura, Alena and Winkler, Laura and Sowa, Mandy and St{\"o}ckle, Michael and Schierack, Peter and Junker, Kerstin and Roggenbuck, Dirk}, title = {Over-Expression of LEDGF/p75 in HEp-2 Cells Enhances Autoimmune IgG Response in Patients with Benign Prostatic Hyperplasia—A Novel Diagnostic Approach with Therapeutic Consequence?}, series = {International Journal of Molecular Sciences}, volume = {24}, journal = {International Journal of Molecular Sciences}, number = {7}, issn = {1422-0067}, doi = {10.3390/ijms24076166}, abstract = {Lens epithelium-derived growth factor splice variant of 75 kDa (LEDGF/p75) is an autoantigen over-expressed in solid tumors and acts as a stress-related transcriptional co-activator. Participation of autoimmune responses in the pathophysiology of benign prostatic hyperplasia (PBH) and a corresponding immunosuppressive therapy by TNFalpha antagonists has been recently suggested. Thus, autoAb testing could aid in the diagnosis of BPH patients profiting from such therapy. We generated CRISPR/Cas9 modified HEp-2 LEDGF knock-out (KO) and HEp-2 LEDGF/p75 over-expressing (OE) cells and examined IgG autoantibody reactivity to LEDGF/p75 in patients with prostate cancer (PCa, n = 89), bladder cancer (BCa, n = 116), benign prostatic hyperplasia (BPH, n = 103), and blood donors (BD, n = 60) by indirect immunofluorescence assay (IFA). Surprisingly, we could not detect elevated binding of autoAbs against LEDGF/p75 in cancer patients, but autoAb reactivity to LEDGF/p75 OE cells in about 50\% of patients with BPH was unexpectedly significantly increased. Furthermore, a line immunoassay enabling the detection of 18 different autoAbs revealed a significantly increased occurrence of anti-dsDNA autoAbs in 34\% of BPH patients in contrast to tumor patients and BD. This finding was confirmed by anti-mitochondrial (mDNA) autoAb detection with the Crithidia luciliae immunofluorescence test, which also showed a significantly higher prevalence (34\%) of anti-mDNA autoAbs in BPH. In summary, our study provided further evidence for the occurrence of autoimmune responses in BPH. Furthermore, LEDGF/p75 over-expression renders HEp-2 cells more autoantigenic and an ideal target for autoAb analysis in BPH with a potential therapy consequence.}, language = {en} }