@misc{BrauneKruegerGengeKammereretal., author = {Braune, Steffen and Kr{\"u}ger-Genge, Anne and Kammerer, Sarah and Jung, Friedrich and K{\"u}pper, Jan-Heiner}, title = {Phycocyanin from Arthrospira platensis as Potential Anti-Cancer Drug: Review of In Vitro and In Vivo Studies}, series = {Life}, volume = {11}, journal = {Life}, number = {2}, doi = {10.3390/life11020091}, pages = {91}, language = {en} } @misc{KruegerGengeJungBrauneetal., author = {Kr{\"u}ger-Genge, Anne and Jung, Conrad H. G. and Braune, Steffen and Harb, K. and Westphal, S. and Kl{\"o}pzig, Stefanie and K{\"u}pper, Jan-Heiner and Jung, Friedrich}, title = {Effect of Arthrospira powders from different producers on the formation of endothelial cell monolayers}, series = {Clinical Hemorheology and Microcirculation}, volume = {79}, journal = {Clinical Hemorheology and Microcirculation}, number = {1}, issn = {1875-8622}, doi = {10.3233/CH-219200}, pages = {193 -- 203}, abstract = {Arthrospira platensis (AP) and some of its derived products have well-established biological activities as antioxidants or as agents to reduce cardiovascular disease risk factors. Furthermore, AP products have gained increasing importance as potential anti-cancer agents. However, the ingredients of the available products vary greatly with the origin, the type of production and processing, which could have significant consequences for their biological effects. Therefore, the composition and biological influence of five distinct AP powders, which were acquired commercially or produced at a public biotechnology institute, were investigated in regard to their endothelialization capacity using a cell impedance- (CI) based measurement method. The study revealed that the AP composition and especially the influence on HUVEC proliferation differed significantly between the five AP powders up to 109\%. Thus, it could be shown that the method used allows the reliable detection of quantitative differences in biological effects of different AP preparations.}, language = {en} } @misc{KuepperKruegerGengeJung, author = {K{\"u}pper, Jan-Heiner and Kr{\"u}ger-Genge, Anne and Jung, Friedrich}, title = {40th conference of the German society for clinical microcirculation and hemorheology (DGKMH)}, series = {Clinical Hemorheology and Microcirculation}, volume = {79}, journal = {Clinical Hemorheology and Microcirculation}, number = {1}, issn = {1875-8622}, doi = {10.3233/ch-219100}, pages = {1 -- 2}, language = {en} } @misc{JungMoranEngeletal., author = {Jung, Ernst Michael and Moran, Valentina Oca{\~n}aa and Engel, Martin and Kr{\"u}ger-Genge, Anne and Stroszczynski, Christian and Jung, Friedrich}, title = {Modified contrast-enhanced ultrasonography with the new high-resolution examination technique of high frame rate contrast-enhanced ultrasound (HiFR-CEUS) for characterization of liver lesions: First results}, series = {Clinical Hemorheology and Microcirculation}, journal = {Clinical Hemorheology and Microcirculation}, issn = {1875-8622}, doi = {10.3233/CH-221449}, pages = {1 -- 16}, language = {en} } @misc{JungBrauneJungetal., author = {Jung, Friedrich and Braune, Steffen and Jung, Conrad H. G. and Kr{\"u}ger-Genge, Anne and Waldeck, Peter and Petrick, Ingolf and K{\"u}pper, Jan-Heiner}, title = {Lipophilic and Hydrophilic Compounds from Arthrospira platensis and Its Effects on Tissue and Blood Cells—An Overview}, series = {Life}, volume = {12}, journal = {Life}, number = {10}, issn = {2075-1729}, doi = {10.3390/life12101497}, language = {en} } @misc{BrauneKruegerGengeKoehleretal., author = {Braune, Steffen and Kr{\"u}ger-Genge, Anne and K{\"o}hler, S. and K{\"u}pper, Jan-Heiner and Jung, Friedrich}, title = {Effects of Arthrospira platensis-derived substances on blood cells}, series = {Clinical Hemorheology and Microcirculation}, volume = {85(2023)}, journal = {Clinical Hemorheology and Microcirculation}, number = {3}, issn = {1875-8622}, doi = {10.3233/CH-229103}, pages = {315 -- 321}, language = {en} } @misc{JungLambyPrantletal., author = {Jung, Friedrich and Lamby, Philipp and Prantl, Lukas and Wiggermann, P. and Jung, Ernst Michael and Kr{\"u}ger-Genge, Anne and Franke, Ralf-Peter}, title = {Post-mortem distribution of Iodinated Contrast Media (ICM) (iodixanol versus iopromide) in the porcine kidney after multiple bolus injections in vivo into the supra-renal aorta}, series = {Clinical Hemorheology and Microcirculation}, volume = {85(2023)}, journal = {Clinical Hemorheology and Microcirculation}, number = {3}, issn = {1875-8622}, doi = {10.3233/CH-229102}, pages = {289 -- 295}, language = {en} } @misc{KruegerGengeKoehlerLaubeetal., author = {Kr{\"u}ger-Genge, Anne and K{\"o}hler, Susanne and Laube, Markus and Haileka, Vanessa and Lemm, Sandy and Majchrzak, Karolina and Kammerer, Sarah and Schulz, Christian and Storsberg, Joachim and Pietzsch, Jens and K{\"u}pper, Jan-Heiner and Jung, Friedrich}, title = {Anti-Cancer Prodrug Cyclophosphamide Exerts Thrombogenic Effects on Human Venous Endothelial Cells Independent of CYP450 Activation—Relevance to Thrombosis}, series = {Cells}, volume = {12}, journal = {Cells}, number = {15}, issn = {2073-4409}, doi = {10.3390/cells12151965}, abstract = {Cancer patients are at a very high risk of serious thrombotic events, often fatal. The causes discussed include the detachment of thrombogenic particles from tumor cells or the adverse effects of chemotherapeutic agents. Cytostatic agents can either act directly on their targets or, in the case of a prodrug approach, require metabolization for their action. Cyclophosphamide (CPA) is a widely used cytostatic drug that requires prodrug activation by cytochrome P450 enzymes (CYP) in the liver. We hypothesize that CPA could induce thrombosis in one of the following ways: (1) damage to endothelial cells (EC) after intra-endothelial metabolization; or (2) direct damage to EC without prior metabolization. In order to investigate this hypothesis, endothelial cells (HUVEC) were treated with CPA in clinically relevant concentrations for up to 8 days. HUVECs were chosen as a model representing the first place of action after intravenous CPA administration. No expression of CYP2B6, CYP3A4, CYP2C9 and CYP2C19 was found in HUVEC, but a weak expression of CYP2C18 was observed. CPA treatment of HUVEC induced DNA damage and a reduced formation of an EC monolayer and caused an increased release of prostacyclin (PGI2) and thromboxane (TXA) associated with a shift of the PGI2/TXA balance to a prothrombotic state. In an in vivo scenario, such processes would promote the risk of thrombus formation.}, language = {en} } @misc{KruegerGengeHarbBrauneetal., author = {Kr{\"u}ger-Genge, Anne and Harb, Kudor and Braune, Steffen and Jung, Conrad H. G. and Westphal, Sophia and B{\"a}r, Stefanie and Mauger, Olivia and K{\"u}pper, Jan-Heiner and Jung, Friedrich}, title = {Effects of arthrospira platensis on human Uumbilical vein endothelial cells}, series = {Life}, volume = {14}, journal = {Life}, number = {10}, editor = {Georgianos, Panagiotis}, publisher = {MDPI}, issn = {2075-1729}, doi = {10.3390/life14101253}, pages = {19}, abstract = {Atherosclerosis is initiated by injury or damage to the vascular endothelial cell monolayer. Therefore, the early repair of the damaged vascular endothelium by a proliferation of neighbouring endothelial cells is important to prevent atherosclerosis and thrombotic events. Arthrospira platensis (AP) has been used as a dietary supplement, mainly due to its high content of vitamins, minerals, amino acids, and pigments such as chlorophylls, carotenoids, and phycocyanin, ingredients with antioxidant, anti-inflammatory, and anti-thrombotic properties. Therefore, in this prospective, placebo-controlled, data-driven, sample-size-estimated in vitro study, we tested whether an aqueous extract of AP at different concentrations (50, 100, and 200 µg/mL) had an effect on the different cellular parameters of human umbilical vein endothelial cells. Therefore, cell impedance measurement and cell proliferation were measured to investigate the monolayer formation. In addition, cell viability, integrity, and metabolism were analysed to evaluate singular cellular functions, especially the antithrombotic state. Furthermore, cell-cell and cell-substrate interactions were observed. The highest proliferation was achieved after the addition of 100 µg/mL. This was consistently confirmed by two independent optical experiments in cell cultures 48 h and 85 h after seeding and additionally by an indirect test. At this concentration, the activation or dysfunction of HUVECs was completely prevented, as confirmed by prostacyclin and interleukin-6 levels. In conclusion, in this study, AP induced a significant increase in HUVEC proliferation without inducing an inflammatory response but altered the hemostasiological balance in favour of prostacyclin over thromboxane, thereby creating an antithrombotic state. Thus, APE could be applied in the future as an accelerator of endothelial cell proliferation after, e.g., stent placement or atherosclerosis.}, language = {en} }