@misc{KolendaBurdukiewiczWimonćetal., author = {Kolenda, Rafał and Burdukiewicz, Michał and Wimonć, Marcjanna and Aleksandrowicz, Adrianna and Ali, Aamir and Szabo, Istvan and Tedin, Karsten and Bartholdson, Scott J. and Pickhard, Derek and Schierack, Peter}, title = {Identification of Natural Mutations Responsible for Altered Infection Phenotypes of Salmonella enterica Clinical Isolates by Using Cell Line Infection Screens}, series = {Applied and Environmental Microbiology}, volume = {87}, journal = {Applied and Environmental Microbiology}, number = {2}, issn = {1098-5336}, doi = {10.1128/AEM.02177-20}, language = {en} } @inproceedings{KolendaBurdukiewiczSzaboetal., author = {Kolenda, Rafał and Burdukiewicz, Michał and Szabo, M. and Orlowska, Aleksandra and Nitschke, J{\"o}rg and B{\"o}hm, Alexander and Schierack, Peter}, title = {Influence of Salmonella FimH sequence variation on binding to pancreatic secretory granule membrane major glycoprotein GP2 of human and porcine origin}, series = {5th ASM Conference on Salmonella, Potsdam, 29.08-01.09.2016}, booktitle = {5th ASM Conference on Salmonella, Potsdam, 29.08-01.09.2016}, publisher = {American Society for Microbiology}, address = {Washington, DC}, pages = {S. 125}, language = {en} } @misc{KhanSidorczukBeckeretal., author = {Khan, Muhammad Moman and Sidorczuk, Katarzyna and Becker, Juliane and Aleksandrowicz, Adrianna and Baraniewicz, Karolina and Ludwig, Christina and Ali, Aamir and Kingsley, Robert A. and Schierack, Peter and Kolenda, Rafał}, title = {Characterization of clumpy adhesion of Escherichia coli to human cells and associated factors influencing antibiotic sensitivity}, series = {Microbiology Spectrum}, journal = {Microbiology Spectrum}, issn = {2165-0497}, doi = {10.1128/spectrum.02606-23}, abstract = {Escherichia coli intestinal infection pathotypes are characterized by distinct adhesion patterns, including the recently described clumpy adhesion phenotype. Here, we identify and characterize the genetic factors contributing to the clumpy adhesion of E. coli strain 4972. In this strain, the transcriptome and proteome of adhered bacteria were found to be distinct from planktonic bacteria in the supernatant. A total of 622 genes in the transcriptome were differentially expressed in bacteria present in clumps relative to the planktonic bacteria. Seven genes targeted for disruption had variable distribution in different pathotypes and nonpathogenic E. coli, with the pilV and spnT genes being the least frequent or absent from most groups. Deletion (Δ) of five differentially expressed genes, flgH, ffp, pilV, spnT, and yggT, affected motility, adhesion, or antibiotic stress. ΔflgH exhibited 80\% decrease and ΔyggT depicted 184\% increase in adhesion, and upon complementation, adhesion was significantly reduced to 13\%. ΔflgH lost motility and was regenerated when complemented, whereas Δffp had significantly increased motility, and reintroduction of the same gene reduced it to the wild-type level. The clumps produced by Δffp and ΔspnT were more resistant and protected the bacteria, with ΔspnT showing the best clump formation in terms of ampicillin stress protection. ΔyggT had the lowest tolerance to gentamicin, where the antibiotic stress completely eliminated the bacteria. Overall, we were able to investigate the influence of clump formation on cell surface adhesion and antimicrobial tolerance, with the contribution of several factors crucial to clump formation on susceptibility to the selected antibiotics.}, language = {en} } @misc{SchierackRoedigerKolendaetal., author = {Schierack, Peter and R{\"o}diger, Stefan and Kolenda, Rafał and Hiemann, Rico and Berger, Enrico and Grzymajlo, Krzysztof and Swidsinski, Alexander and Juretzek, Thomas and Meissner, Dirk and Mydlak, Karsten and Reinhold, Dirk and Nolan, Lisa K. and Roggenbuck, Dirk}, title = {Species-specific and pathotype-specific binding of bacteria to zymogen granule membrane glycoprotein 2 (GP2)}, series = {Gut}, volume = {64}, journal = {Gut}, number = {3}, issn = {1468-3288}, doi = {10.1136/gutjnl-2014-307854}, pages = {517 -- 519}, language = {en} } @misc{KhanKolendaSchieracketal., author = {Khan, Muhammad Moman and Kolenda, Rafał and Schierack, Peter and Weinreich, J{\"o}rg and R{\"o}diger, Stefan and Schierack, Jakob and Stubbe, Michael and Lkhagvasuren, Davaa and Guenther, Sebastian and Schaufler, Katharina}, title = {Investigation of Commensal Escherichia coli Populations of Cormorant Hatchlings in the Absence of Anthropogenic Impacts in Remote Areas of West Mongolia}, series = {Microorganisms}, volume = {9}, journal = {Microorganisms}, number = {2}, issn = {2076-2607}, doi = {10.3390/microorganisms9020372}, language = {en} } @inproceedings{KolendaBurdukiewiczSchiebeletal., author = {Kolenda, Rafał and Burdukiewicz, Michał and Schiebel, Juliane and Szabo, Istvan and Orlowska, Aleksandra and Nitschke, J{\"o}rg and B{\"o}hm, Alexander and Schierack, Peter}, title = {Adhesion of Salmonella to pancreatic secretory granule membrane major glycoprotein GP2 depends on FimH sequence variation}, series = {Abstractbook, Microbiology and Infection, 5th Joint Conference of the DGHM \& VAAM, VAAM Annual Meeting 2017, 69th Annual Meeting of the DGHM}, booktitle = {Abstractbook, Microbiology and Infection, 5th Joint Conference of the DGHM \& VAAM, VAAM Annual Meeting 2017, 69th Annual Meeting of the DGHM}, publisher = {Spektrumverlag}, address = {Heidelberg}, pages = {S. 118}, language = {en} } @misc{GrzymajloUgorskiSuchanskietal., author = {Grzymajlo, Krzysztof and Ugorski, Maciej and Suchanski, Jaroslaw and Kedzierska, Anna E. and Kolenda, Rafał and Jarzab, Anna and Biernatowska, Agnieszka and Schierack, Peter}, title = {The Novel Type 1 Fimbriae FimH Receptor Calreticulin Plays a Role in Salmonella Host Specificity}, series = {Frontiers in Cellular and Infection Microbiology}, volume = {7}, journal = {Frontiers in Cellular and Infection Microbiology}, issn = {2235-2988}, doi = {10.3389/fcimb.2017.00326}, pages = {15}, language = {en} } @misc{KolendaBurdukiewiczSchierack, author = {Kolenda, Rafał and Burdukiewicz, Michał and Schierack, Peter}, title = {A systematic review and meta-analysis of the epidemiology of pathogenic Escherichia coli of calves and the role of calves as reservoirs for human pathogenic E. coli}, series = {Frontiers in Cellular and Infection Microbiology}, volume = {5}, journal = {Frontiers in Cellular and Infection Microbiology}, issn = {2235-2988}, doi = {10.3389/fcimb.2015.00023}, pages = {12}, language = {en} } @misc{KolendaBurdukiewiczSchiebeletal., author = {Kolenda, Rafał and Burdukiewicz, Michał and Schiebel, Juliane and R{\"o}diger, Stefan and Sauer, Lysann and Szabo, Istvan and Orlowska, Aleksandra and Weinreich, J{\"o}rg and Nitschke, J{\"o}rg and B{\"o}hm, Alexander and Gerber, Ulrike and Roggenbuck, Dirk and Schierack, Peter}, title = {Adhesion of Salmonella to Pancreatic Secretory Granule Membrane Major Glycoprotein GP2 of Human and Porcine Origin Depends on FimH Sequence Variation}, series = {Frontiers in Microbiology}, volume = {9}, journal = {Frontiers in Microbiology}, number = {1905}, issn = {1664-302X}, doi = {10.3389/fmicb.2018.01905}, pages = {15}, language = {en} } @misc{KhanAliKolendaetal., author = {Khan, Muhammad Moman and Ali, Aamir and Kolenda, Rafał and Olowe, Olugbenga Adekunle and Weinreich, J{\"o}rg and Li, Ganwu and Schierack, Peter}, title = {The role of AJB35136 and fdtA genes in biofilm formation by avian pathogenic Escherichia coli}, series = {BMC Veterinary Research}, volume = {19}, journal = {BMC Veterinary Research}, issn = {1746-6148}, doi = {10.1186/s12917-023-03672-7}, language = {en} }