@misc{KhanSidorczukBeckeretal., author = {Khan, Muhammad Moman and Sidorczuk, Katarzyna and Becker, Juliane and Aleksandrowicz, Adrianna and Baraniewicz, Karolina and Ludwig, Christina and Ali, Aamir and Kingsley, Robert A. and Schierack, Peter and Kolenda, Rafał}, title = {Characterization of clumpy adhesion of Escherichia coli to human cells and associated factors influencing antibiotic sensitivity}, series = {Microbiology Spectrum}, journal = {Microbiology Spectrum}, issn = {2165-0497}, doi = {10.1128/spectrum.02606-23}, abstract = {Escherichia coli intestinal infection pathotypes are characterized by distinct adhesion patterns, including the recently described clumpy adhesion phenotype. Here, we identify and characterize the genetic factors contributing to the clumpy adhesion of E. coli strain 4972. In this strain, the transcriptome and proteome of adhered bacteria were found to be distinct from planktonic bacteria in the supernatant. A total of 622 genes in the transcriptome were differentially expressed in bacteria present in clumps relative to the planktonic bacteria. Seven genes targeted for disruption had variable distribution in different pathotypes and nonpathogenic E. coli, with the pilV and spnT genes being the least frequent or absent from most groups. Deletion (Δ) of five differentially expressed genes, flgH, ffp, pilV, spnT, and yggT, affected motility, adhesion, or antibiotic stress. ΔflgH exhibited 80\% decrease and ΔyggT depicted 184\% increase in adhesion, and upon complementation, adhesion was significantly reduced to 13\%. ΔflgH lost motility and was regenerated when complemented, whereas Δffp had significantly increased motility, and reintroduction of the same gene reduced it to the wild-type level. The clumps produced by Δffp and ΔspnT were more resistant and protected the bacteria, with ΔspnT showing the best clump formation in terms of ampicillin stress protection. ΔyggT had the lowest tolerance to gentamicin, where the antibiotic stress completely eliminated the bacteria. Overall, we were able to investigate the influence of clump formation on cell surface adhesion and antimicrobial tolerance, with the contribution of several factors crucial to clump formation on susceptibility to the selected antibiotics.}, language = {en} } @misc{KhanKolendaSchieracketal., author = {Khan, Muhammad Moman and Kolenda, Rafał and Schierack, Peter and Weinreich, J{\"o}rg and R{\"o}diger, Stefan and Schierack, Jakob and Stubbe, Michael and Lkhagvasuren, Davaa and Guenther, Sebastian and Schaufler, Katharina}, title = {Investigation of Commensal Escherichia coli Populations of Cormorant Hatchlings in the Absence of Anthropogenic Impacts in Remote Areas of West Mongolia}, series = {Microorganisms}, volume = {9}, journal = {Microorganisms}, number = {2}, issn = {2076-2607}, doi = {10.3390/microorganisms9020372}, language = {en} } @misc{SchmidtKammelTanneretal., author = {Schmidt, Carsten and Kammel, Anne and Tanner, Julian A. and Kinghorn, Andrew B. and Khan, Muhammad Moman and Lehmann, Werner and Menger, Marcus and Schedler, Uwe and Schierack, Peter and R{\"o}diger, Stefan}, title = {A Multiparametic Fluorescence Assay for Screening Aptamer-Protein Interactions Based on Microbeads}, series = {Scientific Reports}, volume = {12}, journal = {Scientific Reports}, issn = {2045-2322}, doi = {10.1038/s41598-022-06817-0}, pages = {10}, language = {en} } @misc{KhanAliKolendaetal., author = {Khan, Muhammad Moman and Ali, Aamir and Kolenda, Rafał and Olowe, Olugbenga Adekunle and Weinreich, J{\"o}rg and Li, Ganwu and Schierack, Peter}, title = {The role of AJB35136 and fdtA genes in biofilm formation by avian pathogenic Escherichia coli}, series = {BMC Veterinary Research}, volume = {19}, journal = {BMC Veterinary Research}, issn = {1746-6148}, doi = {10.1186/s12917-023-03672-7}, language = {en} } @misc{AliKolendaKhanetal., author = {Ali, Aamir and Kolenda, Rafał and Khan, Muhammad Moman and Weinreich, J{\"o}rg and Li, Ganwu and Wieler, Lothar H. and Tedin, Karsten and Roggenbuck, Dirk and Schierack, Peter}, title = {Novel Avian Pathogenic Escherichia coli Genes Responsible for Adhesion to Chicken and Human Cell Lines}, series = {Applied and Environmental Microbiology}, volume = {86}, journal = {Applied and Environmental Microbiology}, number = {20}, issn = {1098-5336}, doi = {10.1128/AEM.01068-20}, language = {en} } @misc{SchierackHeidenKhanetal., author = {Schierack, Peter and Heiden, Stefan E. and Khan, Muhammad Moman and Nikolaus, Lena and Kolenda, Rafał and Stubbe, Michael and Lkhagvasuren, Davaa and R{\"o}diger, Stefan and Guenther, Sebastian and Schaufler, Katharina}, title = {Genomic and Phenotypic Analysis of an ESBL-Producing E. coli ST1159 Clonal Lineage From Wild Birds in Mongolia}, series = {Frontiers in Microbiology}, volume = {11}, journal = {Frontiers in Microbiology}, issn = {1664-302X}, doi = {10.3389/fmicb.2020.01699}, pages = {7}, language = {en} }